Abstracts
Callogenesis was achieved using growth regulators at different concentrations to obtain the best characteristics regarding the calluses texture aspect with the main objective to produce friable calluses from stem explants of Eucalyptus grandis. Stem segments of approximately 1 cm were excised from 30-day seedlings. The treatments were prepared based on the MS medium added with TDZ alone or combined with AIA; 2,4-D; ANA. Each treatment consisted of five repetitions with five explants per plate. After 210 days of in vitro culture, the calluses were evaluated visually and by manipulation for texture. The explants that did not produce calluses were those exposed to treatments with 2,4-D 50.0 µM; TDZ 3.0 µM and in absence of a growth regulator (control).The best protocols for maintenance of friable calluses of stem explants of Eucalyptus grandis under the conditions performed in this work were those using only TDZ.
stem explants; protocols; friable calluses
A calogênese foi efetuada utilizando-se reguladores de crescimento em diferentes concentrações para obtenção das melhores características em relação ao aspecto da textura dos calos, com o objetivo principal de produzir calos friáveis de explantes caulinares de Eucalyptus grandis. Segmentos caulinares de aproximadamente um centímetro foram excisados de mudas de 30 dias. Os tratamentos foram elaborados tendo como base o meio MS adicionado de TDZ isolado ou combinado com AIA, 2,4-D, ANA. Cada tratamento constou de cinco repetições, com cinco explantes por placa. Os calos foram avaliados visualmente e por manipulação quanto à textura, após 210 dias de cultivo in vitro. Os explantes que não produziram calos foram aqueles expostos a tratamentos com 50,0 µM de 2,4-D e 3,0 µM de TDZ, e em ausência de regulador de crescimento (controle). Os melhores protocolos para manutenção de calos friáveis de explantes caulinares de Eucalyptus grandis, nas condições realizadas neste trabalho, foram os que utilizaram apenas TDZ.
explantes caulinares; protocolos; calos friáveis
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Publication Dates
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Publication in this collection
06 Aug 2013 -
Date of issue
Sept 2013
History
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Received
27 Oct 2011 -
Accepted
05 Mar 2013
