Damage Caused by Seed-Feeding Insects in Senna corymbosa: A New Host Plant for the Subfamily Bruchinae

Abstract

In the larval stage, bruchines can cause several damages to the seeds of their host plants due to the consumption of the embryo. The aim of this study was to identify the species of seed-feeding insects in Senna corymbosa (Fabaceae) and quantify the damage caused to seeds. For this purpose, ripe fruits of S. corymbosa were collected monthly from May to August 2014. The fruits were stored in containers to obtain the adult insects and quantify the damage to the seeds. A total of 3,548 seed beetles emerged from the fruits, around 89% belonging to Sennius lateapicalis. Insects consume up to 43.2% of the internal seed content. Moreover, the seed beetles Hymenoptera parasitoids emerged. In this study, seed-feeding insects are recorded for the first time in S. corymbosa. In addition, it contributes to describing fruits and seeds, as well as associated bruchine species and the damage they cause to seeds.

Keywords:
forest entomology; parasitoids; seed predation; seed beetles; Sennius

1. INTRODUCTION AND OBJECTIVES

Several species of Senna have been the focus of studies on seed-feeding insects that cause damage to seeds (Ribeiro-Costa, 1998; Linzmeier et al., 2004; Sari & Ribeiro-Costa, 2005; Sari et al., 2005; Wolowski & Freitas, 2011; Modena et al., 2012; Viana & Ribeiro-Costa, 2013; Viana & Ribeiro-Costa, 2014). Among the granivorous insects, Bruchinae (Coleoptera: Chrysomelidae) can act as agent of natural selection, influencing the population size and the spatial distribution of the plants, due to the partial or total consumption of the seeds, which may harm the reproductive potential of the species (Ribeiro-Costa & Almeida, 2012). As forest seeds have high nutrient content, they favor the development of larvae from several seed-feeding insect species in their interior (Costa et al., 2018), such as legume seeds belonging to Senna corymbosa (Lam.) H. S. Irwin & Barneby (Fabaceae) that are consumed by different species of Amblycerus (Ribeiro-Costa et al., 2018).

The species S. corymbosa is an easily cultivated ornamental plant and has long lasting flowering and abundant fruiting. It is used in urban greening due to its size, which can reach up to three meters in height (Miotto et al., 2008). In Brazil, its flowering occurs in the months of March and April, with fruiting in May (Flora, 2020). This plant species is native from South America, occurring in Argentina, Paraguay, Uruguay and Brazil. However, it is also cultivated in the United States of America, in several countries of Europe, and in China (Irwin & Barneby, 1982; Fortunato, 1999; Lin & Li, 2011). In Brazil, S. corymbosa is found in the Central-west (Mato Grosso do Sul), Southeast (Minas Gerais, Rio de Janeiro and São Paulo) and South (Rio Grande do Sul and Santa Catarina) regions (Reflora, 2020). In Rio Grande do Sul, this plant species is widely found growing in sandy or humid fields, in secondary vegetation, and in forest edges (Rodrigues et al., 2005).

The floral biology of the species is mainly associated with floral visitors belonging to Bombus atratus (Franklin, 1913) (Laporta, 2005). In China, the occurrence of species of the orders Hymenoptera, Lepidoptera, Mantodea, Diptera, and Coleoptera was reported visiting the flowers. Among the Coleoptera, the Chrysomelidae family occasionally visits the flowers; however, they are not considered effective pollinators (Lin & Li, 2011). Their fruits are cylindrical, glabrous, indehiscent, pendulous legumes, 8-10 cm long with brownish seeds (Rodrigues et al., 2005).

Senna corymbosa is related to environmental and ecosystem services being used by farmers as green manure and to improve soil quality. In addition, it has apicultural importance in the production of honey by Apidae Meliponini (Gomes et al., 2019), since the flowers are yellow-gold colored, which attract pollinating insects (Rodrigues et al., 2005). This species also presents medicinal properties among its characteristics (Simões et al., 1995).

Nonetheless, in addition to the apicultural and medicinal importance of this plant species, no studies were found in the literature regarding the interaction of this plant with seed-feeding insects that cause damage to seeds in Rio Grande do Sul, Brazil. This knowledge related to seed-feeding insects is extremely important, since these insects interfere in the dynamics, distribution, life cycle, and evolution of plants due to their consumption and consequent damage to seeds, thus limiting their supply to the environment (Ribeiro-Costa & Almeida, 2009). However, the degree of seed predation attributed to bruchines is quite variable in space and time, and is strictly dependent on the host plant (Klips et al., 2005). Studies on damage caused by granivorous insects in host plants are crucial for the ecological management of economically important plants, such as S. corymbosa. Thus, the aim of this study is to report the occurrence of species of seed-feeding insects in S. corymbosa and quantify the damages caused in their seeds.

2. MATERIALS AND METHODS

An experiment was conducted in a rural property in the municipality of São Sepé, Rio Grande do Sul, Brazil (30°12’24”S; 53°34’30”W). The climate in the region is characterized as humid subtropical (Cfa), according to Köppen classification (Alvares et al., 2013), and the soil belongs to the Vacacaí Mapping Unit (Streck et al., 2008). The municipality is located in the state’s Central Depression, in the phytoecological zone defined as Ecological Tension Area between Seasonal Deciduous Forest and Steppe. According to the Santa Maria/Rio Grande do Sul Automatic Weather Station, located approximately 60 kilometers from the collection site, the temperature and relative humidity averages were 15.1 °C and 86%, respectively, with an accumulated precipitation of 883 mm during the collection period.

To assess the damage caused to seeds by seed-feeding insects, five plants of S. corymbosa of similar size were randomly chosen, all located at the border of a forest remnant of secondary vegetation, 30 meters apart from each other. One sample per month was performed in May, June, July and August 2014. In each sample, around 50 ripe fruits (per tree) were removed from the middle third of the tree crown, individually packaged in clear bags and taken to laboratory for analysis. In all collections performed, the different stages of fruit maturation in green, yellow, and brown colorations were mixed together, forming the lot / tree.

In the laboratory, fruits were sorted according to two methodologies. The first consisted of packaging the fruits in glass containers sealed with voil to obtain adult insect for subsequent identification of fungi growth in the fruits. For this purpose, 10 ripe fruits from each tree in each collection date were stored in the same container, being monitored for three months. Therefore, 200 fruits of S. corymbosa (5 plants x 10 fruits x 4 months) were stored. After adult emergence, 10 out of the 40 stored fruits from each tree were dissected, totalling 50 dissected fruits throughout the study period. They were selected at random, regardless of whether or not insects had emerged. Prior to the dissection procedure, fruits and seeds were measured for length, thickness, and width with an analog caliper. With the dissection, the total number of seeds per S. corymbosa tree (N) was obtained and the percentage (%) of healthy seeds (hs), seeds damaged by insects (sd), and empty and/or without embryo (se) (aborted seeds) were calculated. This classification of the fruit dissection was analyzed without distinguishing the emerged species of granivorous insect, being considered only with or without damage.

Consumed seed content from the fruits stored in glass containers was obtained by the difference in average weight (grams) between healthy and damaged seeds. For this purpose, 10 repetitions of 100 healthy and damaged seeds were weighed (Barreto et al., 2017) in an electronic precision balance (MarK, model 3500). The analysis of variance was performed, followed by the Bonferroni test for averages $(\mathrm{p}\le 0.05)$ with the Sisvar software version 5.3 (Ferreira, 2014).

The second methodology adopted aimed to quantify the number of insects emerged from 136 fruits. The fruits were accommodated in isolation in clear plastic hoses, 15 cm long and 1.5 cm wide, sealed with cotton at the ends to allow air flow and prevent insects from escaping. Based on the number of insects emerged per fruit, the confidence interval of the mean (IC (µ, 0.95)) was determined, considering three classes: Upper: number of individuals greater than the upper limit of the IC at 5%; Middle: number of individuals located within the IC at 5%; and Lower: number of individuals lower than the inferior limit of the IC at 5%. It was determined with the Student’s t distribution.

Thus, 336 mature fruits were stored throughout the assessment period; 200 fruits stored in glass containers and 136 in plastic hoses. The containers and the hoses were maintained in acclimatized culture room during three months with controlled temperature at 25±1ºC and relative humidity 70±10%. After emerging, adults were accommodated in Eppendorf microtubes with alcohol 70% for subsequent assembly, labelling, and identification.

Insects of the order Coleoptera, family Chrysomelidae, subfamily Bruchinae, were deposited in the Departamento de Zoologia, Universidade Federal do Paraná (Identification n. 79). The Coleoptera of the families Mycetophagidae and Latridiidae were deposited in the Departamento de Zoologia, Universidade Federal do Paraná (Registration number: 0113/2014-RN). Hymenopteran parasitoids were deposited in the Instituto Nacional de Pesquisas da Amazônia (INPA).

3. RESULTS AND DISCUSSION

Altogether, 3,548 seed-feeding insects emerged from the 336 ripe fruits of S. corymbosa, being distributed among Sennius lateapicalis (Pic, 1927) (Chrysomelidae) $(\mathrm{n}=3,165;89.2\%)$, Acanthoscelides multimaculatusViana & Ribeiro-Costa, 2013 (Chrysomelidae) $(\mathrm{n}=376;10.6\%)$ and Sennius bondari (Pic, 1929) (Chrysomelidae) $(\mathrm{n}=7;0.2\%)$. In addition, 102 mycophagous beetles belonging to two different species emerged: Lytargus tetraspilotus Le Conte, 1856 (Mycetophagidae) with 84 individuals (82.4%) and Corticaria sp. (Latridiidae) with 18 individuals (17.6%).

In Brazil, 68 species and one subspecies of the genus Acanthoscelides, and 30 species and 2 subspecies of Sennius (Sekerka et al., 2020) were recorded feeding on several species of plants. There are records in the literature that Amblycerus nigromarginatus (Motschulsky, 1874), Amblycerus hoffmanseggi (Gyllenhal, 1833), and Amblycerus submaculatus (Pic, 1927) cause damage to the seeds in S. corymbosa (Ribeiro-Costa et al., 2018).

The species Sennius bondari is registered for the following host plants: Senna splendida (Vog.) Irwin and Barneby, S. multijuga (Rich.) Irwin and Barneby, S. occidentalis (L.) Link, Cassia speciosa Schrad., S. surattensis (L. L. Burman) Irwin and Barneby, S. bicapsularis (L.) Roxb., S. pendula (Willd.) var. advena (Vog.) Irwin and Barneby, S. pistaciifolia (H.B.K.) Irwin and Barneby, S. alata (L.) Roxburgh, S. neglecta var. oligophylla, S. neglecta var. neglecta, S. appendiculata (Vogel) Wiersema and S. macranthera (DC. ex Collad.) H.S.Irwin & Barneby (Bondar, 1937; Silva et al., 1968; Johnson, 1984; Macêdo et al., 1992, Ribeiro-Costa, 1998, Ribeiro-Costa & Reynaud, 1998, Linzmeier et al., 2004, Viana & Ribeiro-Costa, 2013). This species is distributed in Colombia (Valle del Cauca), Venezuela (Carabobo), Bolivia (Santa Cruz), and Brazil (Bahia, Rio de Janeiro, Minas Gerais, São Paulo, Mato Grosso, Mato Grosso do Sul, Paraná, Rio Grande do Norte, Piauí, Distrito Federal and Espírito Santo) (Viana & Ribeiro-Costa, 2013). For the species S. lateapicalis, there are records in the host plants S. splendida and S. bicapsularis (Bondar, 1937; Bosq, 1943; Silva et al., 1968) distributed in Brazil (Rio de Janeiro and Santa Catarina) (Macêdo et al., 1992). Acanthoscelides multimaculatus with distribution published for Brazil (Rio de Janeiro, Mato Grosso do Sul and Paraná) has already been registered, causing damage to S. neglecta var. oligophylla, S. neglecta var. neglecta and S. occidentalis (Viana & Ribeiro-Costa, 2013). This data suggests our study was the first to record these insects associated to S. corymbosa in Rio Grande do Sul, Brazil.

Larvae of seed beetles feed on the cotyledon during their development, and in their last instar before pupating, they compact excrement and food remains to form a pupal chamber (Ribeiro-Costa, 1998) (Figure 1). This result is similar to the one found by Linzmeier et al. (2004) for S. bondari in seeds of S. macranthera.

Figure 1
(A) Host plant Senna corymbosa. The arrows indicates some fruits. (B) Detail of healthy seed of Senna corymbosa, (C) pupal chamber, (D) seed consumed with adult insect exit hole, (E) and totally consumed seed, (F) dorsal view of Sennius lateapicalis (Pic, 1927), (G) dorsal view of Sennius bondari (Pic, 1929), (H) dorsal view of Acanthoscelides multimaculatusViana & Ribeiro-Costa, 2013.

The flowering phenophase of the host plants provides food for adults, and the fruiting phenophase provides substrate for oviposition and larval development. Thus, adult bruchines consume pollen, while the larvae obtain nutrients for their complete development, consuming the internal seed content (Rossi et al., 2011). For the species found in this study, S. bondari consumes the internal content of a single seed (Ribeiro-Costa, 1998) in seeds of Senna alata (L.) Roxb. and S. macranthera (Linzmeier et al., 2004). However, there are species that need more than one seed to complete their development (Ribeiro-Costa & Almeida, 2012).

Bruchines emerged from the fruits collected during the four months of evaluation. It is worth mentioning that it was not verified whether the three species of bruchines occur in the same fruit. Moreover, the separation of the damaged fruits by each species of coleoptera suggests that future studies may fill this gap.

The average length of the fruits was of 8.34 cm (minimum 5 - maximum 14,5 cm), with 0.75 cm thickness (minimum 0.5, maximum 1 cm) and 0.72 cm width (minimum 0.5, maximum 0.9 cm). The seeds presented average length, thickness, and width of 0.57, 0.23 and 0.42 cm, respectively. The number of seeds ranged from 21 to 32 per fruit. 10 to 19 seeds per fruit were healthy without the presence of insects, while 1 to 13 were damaged, and 1 to 8 seeds per fruit were empty and/or without its embryo. This variation in the number of damaged seeds per fruit can compromise the reproduction of the host plant, as emphasized by Sousa-Lopes et al. (2019), who found, for example, that seeds of infested fruits have less resource allocation and, consequently, worse germination rate than healthy seeds of non-infested fruits. After the dissection of 50 fruits, it was found that each fruit had an average of 24.04 seeds (minimum 8, maximum 38), and the average of damaged seeds was 6.6 (minimum 0, maximum 26). Hence, seed beetles can consume around 27% of S. corymbosa seeds.

From the total number of seeds analyzed $(\mathrm{n}=1,238)$, 57% were healthy, 27% were damaged by seed beetles, and 16% were empty and/or without embryo (Table 1). The percentage of damaged seeds found in this study was high compared to the result obtained by Sari and Ribeiro-Costa (2005), who found 12.5% of S. multijuga seeds damaged by Sennius crudelis Ribeiro Costa & Reynaud, 1998, Sennius puncticollis (Fåhraeus, 1839) and Sennius nappiRibeiro-Costa & Reynaud, 1998. Nevertheless, the percentage of seeds damaged by bruchines may vary considerably between species, as demonstrated in the study by Rodrigues et al. (2012), who found a predation rate which varied from 0.62% in Senna hirsuta (L.) H.S.Irwin & Barneby (Fabaceae) by Acanthoscelides sp. to 42.08% in Leucaena leucocephala (Lam.) of Wit (Fabaceae) predated by Acanthoscelides macrophthalmus (Schaeffer, 1907). By studying the species L. leucocephala, Raghu et al. (2005) found that the percentage in seeds pre-dispersed by A. macrophthalmus varied from 10.75% to 53.54% of seeds damaged in pods that remained on the tree for one and four months, respectively.

Table 1
Total number of seeds (ts), healthy seeds (hs), seeds damaged (sd), seeds empty and/or without embryo (se) and the respective percentages per tree of Senna corymbosa in the municipality of São Sepé, RS. ($\mathrm{N}=10$ per tree).

Tree	ts	hs	sd	se
A	260	119 (46%)	115 (44%)	26 (10%)
B	315	103 (33%)	133 (42%)	79 (25%)
C	208	117 (56%)	43 (21%)	48 (23%)
D	253	193 (76%)	32 (13%)	28 (11%)
E	202	178 (88%)	9 (4%)	15 (7%)
Total	1,238	710 (57%)	332 (27%)	196 (16%)

When calculating the percentage of seeds that were empty and/or without embryo, and damaged seeds, the latter with a high probability of not germinating, the value is around 43% of seed loss. When considering that several other factors can occasionally decrease the germinative potential such as pathogens and unfavourable environmental conditions, this percentage may become significant (Sari & Ribeiro-Costa, 2005).

While analyzing the confidence interval for the number of emerged seed beetles, from 136 fruits, up to eight bruchines emerged in 118 fruits, up to sixteen adults emerged in 15 fruits, and more than seventeen bruchines emerged in three fruits (Table 2).

Table 2
Confidence interval for the number of insects emerged per fruit of Senna corymbosa, in the municipality of São Sepé, RS.

Confidence Intervals (IC (µ, 0.95))	Number of fruits
Lower (0 - 8 insects emerged)	118
Middle (9 - 16 insects emerged)	15
Upper (17 - 24 insects emerged)	3

The average weight of 100 seeds differed statistically between healthy and damaged seeds $({\mathrm{F}}_{1,18}=532.188,\mathrm{p}<0.0001)$. The average weight of healthy and damaged seeds was 2.59g and 1.47g, respectively. Thus, an average of 1.12g (43.2%) of the internal seed content is consumed by the insects.

Oliveira & Costa (2009) found similar results, also with Fabaceae, for seeds of Acacia mearnsii De Wild. damaged by Stator limbatus (Horn, 1873) in which cotyledon consumption was 39.6%. Boscardin et al. (2012) found 41.3% of seed mass of Vachellia caven (Molina) Seigler & Ebinger consumed by Pseudopachymerina spinipes (Erichson, 1833).

In addition to seed beetles, Hymenoptera parasitoids emerged, being identified as Bracon sp. (Braconidae) with 40 individuals; Inostemma striaticornu Buhl, 2002 (Platygastridae) with six individuals; and Omeganastatus sp. (Eupelmidae) with 15 individuals.

4. CONCLUSIONS

This was the first occurrence of seed-feeding insects in S. corymbosa recorded in Rio Grande do Sul, Brazil.

The species of seed-feeding insects Sennius lateapicalis, Sennius bondari, and Acanthoscelides multimaculatus (Bruchinae: Chrysomelidae) occur in S. corymbosa consuming up to 43.2% of internal seed content.

Important Hymenoptera parasitoids Bracon sp. (Braconidae), Inostemma striaticornu (Platygastridae), and Omeganastatus sp. (Eupelmidae) were reported.

Further studies on the impact of insect species on germination and seedling quality of S. corymbosa are needed.

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Edited by

Associate editor: Natália Corniani https://orcid.org/0000-0001-7342-5171

Publication Dates

• Publication in this collection
  07 Aug 2020
• Date of issue
  2021

History

• Received
  03 Feb 2020
• Accepted
  10 July 2020