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Germination and initial development of forest species under the action of catechin, presents in seeds of Sesbania virgata (Cav.) Pers. (Fabaceae)1 1 Part of the Master’s Dissertation of the first Author

Germinação e desenvolvimento inicial de espécies florestais sob a ação da catequina, presente nas sementes de Sesbania virgata (Cav.) Pers. (Fabaceae)

ABSTRACT

Sesbania virgata is a shrub that occurs in riparian forests, especially in Cerrado and Atlantic Forest. It is considered superdominant due to its rapid growth and high soil cover. Its seeds release allelochemicals during imbibition, affecting the germination and initial growth of other species. The aim of this study was to evaluate the phytotoxic effect of compounds found in S. virgata seeds on co-occurrent species from different successional stages. The species were co-germinated with S. virgata seeds and irrigated with S. virgata integument extracts, in laboratory, greenhouse, and field. The germination rate, germination speed, germination speed index, and seedling growth of the species were evaluated. Phytochemicals released by S. virgata seeds were not able to inhibit the germination of the co-occurrent forest species in field, but significantly reduced their initial growth. The results suggest that resistance to allelochemicals is not linked to the successional stage of a species but is determined by specific characteristics that guarantee the ability to tolerate the phytotoxins released by the seeds of S. virgata. In addition, the results also suggest that catechin and other compounds found in seeds may be responsible for the inhibitory potential of S. virgata plants.

Keywords:
allelopathy; plant establishment; successional stages; superdominat species

RESUMO

Sesbania virgata é um arbusto que ocorre em matas ciliares, principalmente no Cerrado e Mata Atlântica. É considerada superdominante devido ao rapido crescimento e alta cobertura do solo. Suas sementes liberam aleloquímicos durante a embebição, afetando germinação e crescimento inicial de outras espécies. O objetivo desse estudo foi avaliar o efeito fitotóxico de compostos encontrados em sementes de S. virgata, em espécies co-ocorrentes de diferentes estágios sucessionais. As espécies foram co-germinadas com sementes de S. virgata e irrigadas com extratos de tegumento de S. virgata, em laboratório, casa de vegetação e campo. Foram avaliados a taxa de germinação, a velocidade de germinação, o índice de velocidade de germinação e o desenvolvimento inicial das espécies. Fitoquímicos liberados pelas sementes de S. virgata não inibiram a germinação das espécies co-ocorrentes no campo, mas reduziram significativamente o desenvolvimento inicial destas. Os resultados sugerem que a resistência a aleloquímicos não está ligada ao estágio sucessional de uma espécie, mas é determinada por características específicas que garantem a capacidade de tolerar as fitotoxinas liberadas pelas sementes de S. virgata. Além disso, os resultados também sugerem que catequina e outros compostos encontrados nas sementes podem garantir o potencial inibitório de S. virgata.

Plavras-chave:
alelopatia; estabelecimento vegetal; estagios sucessionais; espécie superdominante

Introduction

Plant populations with high propagation rates and rapid growth create serious ecological problems in natural environments because they form massive monocultures. Successful plant growth in a new habitat depends on individual characteristics of each species, including high photosynthetic efficiency, high growth rate, tolerance to defoliation and herbivory, high capacity for regeneration and reproduction, fast reproductive cycle, high seed yield, and high germination capacity (Barbosa et al. 2008Barbosa, E.G., Pivello, V.R. & Meirelles, S.T. 2008. Allelopathic evidence in Brachiaria decumbens and its potential to invade the Brazilian cerrados. Brazilian archives of biology and technology 51(4): 625-631.). Allelopathy has been recognized as a key biological mechanism in the success of plant species in new environments, providing adaptive advantages for some species and facilitating their establishment and survival (Lorenzo et al. 2013Lorenzo, P., Hussain, M.I. & González, L. 2013. Role of allelopathy during invasion process by alien invasive plants in terrestrial ecosystems. In: Cheema Z., Farooq M., Wahid A. (eds) Allelopathy. Springer, Berlin, Heidelberg, pp. 3-21., Trezzi et al. 2016Trezzi, M.M., Vidal, R.A., Balbinot Junior, A.A., von Hertwig Bittencourt, H. & Silva Souza Filho, A.P. 2016. Allelopathy: driving mechanisms governing its activity in agriculture. Journal of Plant Interactions 11(1): 53-60.). Allelopathic mechanisms can influence the competitive ability and dominance of individual plants in plant communities, affecting the recruitment and establishment of native species during succession in reforestation projects (Abhilasha et al. 2008Abhilasha, D., Quintana, N., Vivanco, J.M. & Joshi, J. 2008. Do allelopathic compounds in invasive Solidago canadensis s.l. restrain the native European flora? Journal of Ecology 96(5): 993-1001., Alford et al. 2009Alford, É.R., Vivanco, J.M. & Paschke, M.W. 2009. The effects of flavonoid allelochemicals from knapweeds on legume-rhizobia candidates for restoration. Restoration Ecology 17(4): 506-514., Lorenzo et al. 2013Lorenzo, P., Hussain, M.I. & González, L. 2013. Role of allelopathy during invasion process by alien invasive plants in terrestrial ecosystems. In: Cheema Z., Farooq M., Wahid A. (eds) Allelopathy. Springer, Berlin, Heidelberg, pp. 3-21.).

Both intra- and interspecific interference between plants may be caused by allelochemicals (Reigosa et al. 1999Reigosa, M.J., Sánchez-Moreiras, A., González, L. 1999. Ecophysiological approach in allelopathy. Critical Reviews in Plant Sciences 18(5): 577-608., Callaway et al. 2005Callaway, R.M., Ridenour, W.M., Laboski, T., Weir, T. & Vivanco, J.M. 2005. Natural selection for resistance to the allelopathic effects of invasive plants. Journal of Ecology 93(3): 576-583.), which are secondary metabolites (Weir et al. 2004Weir, T.L., Park, S-W. & Vivanco, J.M. 2004. Biochemical and physiological mechanisms mediated by allelochemicals. Current Opinion in Plant Biology 7: 472-479., Reigosa & Gonzales 2006Reigosa, M.J. & González, L. 2006. Forest ecosystems and allelopathy. In: Reigosa, M., Pedrol, N., González L. (eds) Allelopathy. Springer, Dordrecht, pp. 451-463.; Tharayil et al. 2009Tharayil, N., Bhowmik, P., Alpert, P., Walker, E., Amarasiriwardena, D. & Xing, B. 2009. Dual purpose secondary compounds: phytotoxin of Centaurea diffusa also facilitates nutrient uptake. New Phytologist 181(2): 424-434.). These substances are found in all plant organs (Anaya 1999Anaya, A.L. 1999. Allelopathy as a tool in the management of biotic resources in agroecosystems. Critical reviews in plant sciences 18 (6): 697-739.) including seeds, which release allelochemicals after the onset of imbibition and can contribute to the invasive behavior of some species (Ens et al. 2009Ens, E.J., French, K. & Bremner, J.B. 2009. Evidence for allelopathy as a mechanism of community composition change by an invasive exotic shrub, Chrysanthemoides monilifera spp. rotundata. Plant and Soil 316(1-2): 125-137., Holzmueller & Jose 2011Holzmueller, E.J. & Jose, S. 2011. Invasion success of cogongrass, an alien C4 perennial grass, in the southeastern United States: exploration of the ecological basis. Biological invasions 13(2): 435-442., Iqbal & Fry 2012Iqbal, A. & Fry, S. 2012. Potent endogenous allelopathic compounds in Lepidium sativum seed exudate: effects on epidermal cell growth in Amaranthus caudatus seedlings. Journal of Experimental Botany 63: 2595-2604.).

The hypothesis that allelochemicals produced by invasive species provide a competitive advantage over native species proposes that plants co-occurring with species that exude phytotoxins develop resistance mechanisms to these allelochemicals (Inderjit et al. 2008Inderjit, Pollock, J.L., Callaway, R.M. & Holben, W. 2008. Phytotoxic effects of (±)- catechin in vitro, in soil, and in the field. Plos One 3: 1-11., He et al. 2009He, W-M., Feng, Y., Ridenour, W.M., Thelen, G.C., Pollock, J.L., Diaconu, A. & Callaway, R.M. 2009. Novel weapons and invasion: biogeographic differences in the competitive effects of Centaurea maculosa and its root exudates (±)-catechin. Oecologia 159: 803-815. , Thorpe et al. 2009Thorpe, A.S., Thelen, G.C., Diaconu, A. & Callaway, R.M. 2009. Root exudate is allelopathic in invaded community but not in native community: field evidence for the novel weapons hypothesis. Journal of Ecology 97: 641-645., Weidenhamer & Callaway 2010Weidenhamer, J.D. & Callaway, R.M. 2010. Direct and indirect effects of invasive plants on soil chemistry and ecosystem function. Journal of Chemical Ecology 36: 59-69.). However, field studies showing that plant toxic substances accumulate in the environment to levels capable of inhibiting the growth of other plants are lacking (Weidenhamer 2005Weidenhamer, J.D. 2005. Biomimetic measurement of allelochemical dynamics in the rhizosphere. Journal of Chemical Ecology 3: 221-236.). The allelopathic effects observed in bioassays performed under controlled conditions are difficult to be extrapolated to natural conditions, since in a controlled condition factors that may interfere in the results are not considered, such as soil and climate , genetic changes and biotic interactions (Inderjit & Weston 2000Inderjit & Weston, L.A. 2000. Are laboratory bioassays for allelopathy suitable for prediction of field responses? Journal of Chemical Ecology 26(9): 2111-2118., Weir et al. 2004Weir, T.L., Park, S-W. & Vivanco, J.M. 2004. Biochemical and physiological mechanisms mediated by allelochemicals. Current Opinion in Plant Biology 7: 472-479., Trezzi et al. 2016Trezzi, M.M., Vidal, R.A., Balbinot Junior, A.A., von Hertwig Bittencourt, H. & Silva Souza Filho, A.P. 2016. Allelopathy: driving mechanisms governing its activity in agriculture. Journal of Plant Interactions 11(1): 53-60.).

Some secondary metabolites with allelopathic potential such as phenolic compounds are found in the seed tegument of plants in the genus Sesbania (Fabaceae) and may be quickly released during the imbibition process. In Sesbania drummondii (Rydb) Cory, the allelochemical (+)-catechin was found in greater amounts compared to (−)-catechin, whereas the opposite was observed in Sesbania vesicaria (Jacq.) Ell. in which (−)-catechin predominates (Ceballos et al. 1998Ceballos, L., Hossaert-Mckey, M., Mckey, D. & Andary, C. 1998. Rapid deployment of allelochemicals in exudates of germinating seeds of Sesbania (Fabaceae): roles of seed anatomy and histolocalization of polyphenolic compounds in anti-pathogen defense of seedlings. Chemoecology 8: 141-151.).

The application of commercial (+)-catechin to seeds of native and crops species were shown to affect root growth in all tested species (Simões et al. 2008Simões, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.R., Vivanco, J.M. & Braga, M.R. 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology 34: 681-687., Mignoni et al. 2017Mignoni, D.S.B., Simões, K. & Braga, M.R. 2017. Potential allelopathic effects of the tropical legume Sesbania virgata on the alien Leucaena leucocephala related to seed carbohydrate metabolism. Biological Invasions: 1-16.). The flavonoid (+)-catechin is the main allelochemical released by Sesbania virgata (Cav.) Pers. seeds (Simões et al. 2008Simões, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.R., Vivanco, J.M. & Braga, M.R. 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology 34: 681-687.). The substance is produced in the seed coat and released in high concentrations (235 mg/seed) after 24 hours of imbibition. In the same study, S. virgata was shown to inhibit seedling growth in Arabidopsis thaliana (L.) Heynh. and Oryza sativa L.

The wand riverhemp Sesbania virgata (Cav.) Pers. (Fabaceae-Faboideae) is a fast-growing pioneer shrub native to South America (Molle & Tiné 2009Molle, F.R.D. & Tiné, M.A.S. 2009. Catabolismo de sacarose durante a mobilização do galactomanano e sua importância na estratégia de sobrevivência de plântulas de Sesbania virgate (Cav.) Pers. Hoehnea 36(2): 259-268.), and found in south, southeast, and central-west Brazil, mainly in the Atlantic Forest and Cerrado biomes (Florentino et al. 2009Florentino, L.A., Guimarães, A.P., Rufini, M., da Silva, K. & Moreira, F.M.S. 2009. Sesbania virgata stimulates the occurrence of its microsymbiont in soils but does not inhibit microsymbionts of other species. Scientia Agricola 66: 667-676.). The species has fast germination rates, high development index, and high ground cover potential (Coutinho et al. 2005Coutinho, M.P., Carneiro, J.G.A., Barroso, D.G., Rodrigues, L.A., Figueiredo, F.A.M.M.A., Mendonça, A.V.R. & Novaes, A.B. 2005. Crescimento de mudas de Sesbania virgata (Cav.) Pers. plantadas em uma área degradada por extração de argila. Floresta 35: 231-239., Zanandrea et al. 2009Zanandrea, I., Alves, J.D., Deuner, S., Goulart, P.F.P., Henrique, P.C. & Silveira, N.M. 2009. Tolerance of Sesbania virgata plants to flooding. Australian Jornal of Botany 57: 661-669.). Sesbania virgata has been used in the restoration of degraded areas and revegetation of gallery forests (Coelho et al. 2019Coelho, L.C.S., Mignoni, D.S.B, Silva, F.S.B. & Braga, M.R. 2019. Seed exudates of Sesbania virgate (Cav.) Pers. stimulate the asynbiotic phase of the arbuscular mycorrizal fungus Gigaspora albina Becker & Hall. Hoehnea 46(1): e272018. Epub 04 de abril de 2019.https://doi.org/10.1590/2236-8906-27/2018.
https://doi.org/10.1590/2236-8906-27/201...
) due to its hardiness, tolerance to low fertility soils, and competitive ability against other plant species (Moreira et al. 2006Moreira, F.M.S., Cruz, L., Faria, S.M., Marsh, T., Martínez-Romero, E., Oliveira Pedrosa, F., Pitard, R.M., Young, J.P.W. 2006. Azorhizobium doebereinerae sp. nov. microsymbiont of Sesbania virgata (Cav.) Pers. Systematic and applied microbiology 29(3): 197-206., Florentino et al. 2009Florentino, L.A., Guimarães, A.P., Rufini, M., da Silva, K. & Moreira, F.M.S. 2009. Sesbania virgata stimulates the occurrence of its microsymbiont in soils but does not inhibit microsymbionts of other species. Scientia Agricola 66: 667-676., Branzini et al. 2012Branzini, A., González, R.S. & Zubillaga, M. 2012. Absorption and translocation of copper, zinc and chromium by Sesbania virgata. Journal of environmental management 102: 50-54.). Due to its aggressiveness in natural habitats, S. virgata is considered a superdominant plant with invasive behavior (Matos & Pivello 2009Matos, D.M.S. & Pivello, V.R. 2009. O impacto das plantas invasoras nos recursos naturais de ambientes terrestres- Alguns casos brasileiros. Ciência e Cultura 61 (1): 27-30., Dalmagro et al. 2016Dalmagro, H.J., Lathuillière, M.J., Vourlitis, G.L., Campos, R.C., Pinto, O.B., Johnson, M.S., Ortíz, C.E., Lobo, F.D.A. & Couto, E.G. 2016. Physiological responses to extreme hydrological events in the Pantanal wetland: heterogeneity of a plant community containing super‐dominant species. Journal of Vegetation Science 27(3): 568-577.).

El Id et al. (2015)El Id, V.L., Costa, B.V., Mignoni, D.S.B., Veronesi, M.B., Simões, K., Braga, M.R. & Santos Junior, N.A. 2015. Phytotoxic effect of Sesbania virgata (Cav.) Pers. on seeds of agronomic and forestry species. Journal of Forestry Research 26: 339-346. showed that the germination and initial development of agronomic species were significantly affected by S. virgata seed exudates. In contrast, the germination and development of non-pioneer native species, which may co-occur with S. virgata in its natural habitat, were only moderately affected. In addition, tests performed with leaf extracts of S. virgata showed that the germination rate and initial development of forest and agronomic species were not reduced. These results indicate that seeds rather than leaves and branches are the major source of allelochemicals in S. virgata.

Few studies have investigated the effect of S. virgata allelochemicals on the seed physiology of other co-occurrent plant species (El Id et al. 2015El Id, V.L., Costa, B.V., Mignoni, D.S.B., Veronesi, M.B., Simões, K., Braga, M.R. & Santos Junior, N.A. 2015. Phytotoxic effect of Sesbania virgata (Cav.) Pers. on seeds of agronomic and forestry species. Journal of Forestry Research 26: 339-346.), especially under field conditions. From the hypothesis that invasive species producing allelochemicals would have greater competitive advantages under native species, we hypothesized that native and co-occurring species with native species producing allelochemicals would have, over time, acquired mechanisms capable of tolerating such phytotoxicity, due to the common occurrence history. Thus, to test this hypothesis we investigated, in the laboratory, greenhouse and field, the inhibitory effect of S. virgata seeds on the germination and initial growth of species that co-occur with S. virgata in its natural habitat, which is a native species with allelopathic potential.

Material and Methods

Biological material - Seeds of S. virgata were harvested from three different natural populations located in Lavras, Minas Gerais, Brazil (45°00’25’’W 21°13’35’’S, 45°00’24’’W 21°13’30’’S, 45°00’39’’ 21°13’11’’S and altitude of 830m above sea level). The characteristic forest formation of region can be considered as a transition between Montana and Cerrado Seasonal Semideciduous Forest, widely fragmented and in several seral stages (Pereira et al. 2010Pereira, I.M., van den Berg, E., Pinto, L.V.D.A., Higuchi, P., & Carvalho, D.A.D. 2010. Avaliação e proposta de conectividade dos fragmentos remanescentes no campus da Universidade Federal de Lavras, Minas Gerais. Cerne 16(3): 305-321.). The collected seeds were stored in a refrigerator for 30 days, until the beginning of experiments.

To test the potential inhibition of S. virgata seeds, three co-occurrent species from different successional stages were selected: Mimosa bimucronata (DC.) Kuntze (pioneer), Peltophorum dubium (Spreng.) Taub. (light-demanding climax), and Copaifera langsdorffii Desf. (shade-tolerant climax). These species were selected based on a floristic survey that identified all species with plant height > 1.2 m located within a 10-m radius of S. virgata individuals. Seeds of M. bimucronata and P. dubium were obtained from stored seed lots of the seed bank at the Seed Research Center, Institute of Botany of São Paulo, and C. langsdorffii seeds were collected from trees grown in the Botanical Garden of São Paulo, São Paulo, Brazil.

Co-germination assays with S. virgata seeds - To all seeds to germinate homogeneously and thus, for the tests species to come into contact with substances from S. virgata, the seeds with coat dormancy were scarified. To overcome seed coat dormancy, S. virgata, P. dubium, and C. langsdorffii seeds (Cruz & Carvalho 2006Cruz, E.D. & Carvalho, J.U.D. 2006. Methods of overcoming dormancy in Schizolobium amazonicum Huber ex Ducke (Leguminosae-Caesalpinioideae) seeds. Revista Brasileira de Sementes 28(3): 108-115., Pereira et al. 2014Pereira, W.V.S., Faria, J.M.R., Tonetti, O.A.O. & Silva, E.A.A.D. 2014. Loss of desiccation tolerance in Copaifera langsdorffii Desf. seeds during germination. Brazilian Journal of Biology 74(2): 501-508., Shreelalitha et al. 2015Shreelalitha, S.J., Sridhar, K.R. & Sukesh, S. 2015. Seed dormancy and germination in two wild genotypes of Sesbania of the southwest mangroves in India. Journal of Agricultural Technology 11(4): 895-902.) were hand-scarified with sandpaper (P60). Mimosa bimucronata seeds were not scarified due to the absence of an impermeable tegument. Next, M. bimucronata, P. dubium, and C. langsdorffii seeds were disinfected with sodium hypochlorite (10%) for 10 min and washed thoroughly with distilled water. Sesbania virgata seeds were not disinfected to not affect their exudates. The S. virgata seeds were selected from the mixture of seeds from three matrices of S. virgata, which produce catechin.

In the laboratory assay, one seed of M. bimucronata was co-germinated with 0 (control), 5, or 10 S. virgata seeds in 9-cm Petri dishes containing filter paper moistened with 5 mL of distilled water (El Id et al. 2015El Id, V.L., Costa, B.V., Mignoni, D.S.B., Veronesi, M.B., Simões, K., Braga, M.R. & Santos Junior, N.A. 2015. Phytotoxic effect of Sesbania virgata (Cav.) Pers. on seeds of agronomic and forestry species. Journal of Forestry Research 26: 339-346.). The control treatment consisted of four replicates, with five Petri dishes per replicate, each plate containing one seed of the tested species, totaling five seeds of each species per replicate, that is, 20 seeds per treatment. This experimental design was applied in co-germination treatments with 5 and 10 S. virgata seeds.

The same treatments and conditions were applied for P. dubium and C. langsdorffii. Plates were maintained in a germination chamber at 25 °C with continuous light (515.87 lux), irrigated with 3‒5 mL of distilled water every 48 h, and evaluated daily for 15 days, from the germination of each species. At the end of the experiment, germination rate (G%), germination speed (GS) (Borghetti and Ferreira 2004Borghetti, F., & Ferreira, A. G. 2004. Interpretação de resultados de germinação. Germinação: do básico ao aplicado. Porto Alegre: Artmed: 209-222.) and germination speed index (GSI) (Maguire 1962Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science 2: 176-177.), and root length were evaluated. The same germination treatments described above were used in greenhouse and field conditions, replacing Petri dishes by pots and pits, respectively. In the greenhouse, seeds were sown in pots (415 mL) containing the soil from the place where the field experiments were conducted. The pots were watered daily with 15 mL of distilled water and, after seven days, G%, GS and GSI were determined. The experiment was maintained for 90 days to evaluate plant development, measured as plant height, shoot diameter, and shoot dry weight. Under field conditions, the bioassays were conducted in an area near to Atlantic forest fragments, located at Institue of Botany in São Paulo, SP. Seeds were sown in 15-cm diameter pits and irrigated daily with 15 mL of water. The experiment was evaluated daily and G%, GS and GSI were calculated after seven days, from the germination of each species The field assays were maintained for 90 days for determination of plant height, shoot diameter, and shoot dry weight. The same experimental design used in the laboratory was applied in greenhouse and field treatments

Sesbania virgata seed coat extracts - To produce the aqueous extracts used for irrigation, 1,000 S. virgata seeds were scarified as described above and imbibed in 300 mL of distilled water in dark polystyrene plastic boxes (11 × 11 × 3.5 cm) for 8 h at 25 °C. After drying, seed coats were removed from seeds, frozen, freeze-dried, and powdered in a bead mill. The powdered material was suspended in distilled water (1 mg mL−1), shaken on a plate shaker at maximum speed (1500 RPM) for 45 min, filtered, diluted at 0.1, 0.5, and 1.0% (w/v), and used for irrigation. These concentrations were obtained from previous experiments (Simões 2008Simões, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.R., Vivanco, J.M. & Braga, M.R. 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology 34: 681-687., El Id et al. 2015El Id, V.L., Costa, B.V., Mignoni, D.S.B., Veronesi, M.B., Simões, K., Braga, M.R. & Santos Junior, N.A. 2015. Phytotoxic effect of Sesbania virgata (Cav.) Pers. on seeds of agronomic and forestry species. Journal of Forestry Research 26: 339-346.), and the extracts were produced from the seeds from the matrix that presented higher amount of catechin. In addition to distilled water, an aqueous solution of 1 mg mL−1 (+)-Catechin (Sigma-Aldrich) was used as positive control.

Catechin quantification - For the quantification of catechin from seeds, S. virgata seed exudates were collected at six-hour intervals for 48 h (6, 12, 18, 24, 30, 36, 42, and 48 h) as three replicates from 50 seeds per replicate at each time interval. The seeds were scarified as described above, immersed in 250 mL of distilled water in dark polystyrene plastic boxes (11 × 11 × 3.5 cm), and placed into climate chamber at 25 °C. Seed exudates were collected every 6 h and stored in a freezer until analysis. The presence of catechin was confirmed after solvent partition of the extracts with ethyl acetate (Simões et al. 2008Simões, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.R., Vivanco, J.M. & Braga, M.R. 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology 34: 681-687.) and subsequent analysis by HPLC. Aliquots of the extracts (20 µL) were analyzed using a C18 column on an Agilent 1220 Infinity LC DAD high performance liquid chromatographer with a linear gradient of water and methanol acidified with 1% acetic acid (20‒100% methanol) at a flow rate of 0.8 mL min−1. (+)-Catechin was used as standard at a concentration of 0.0625, 0.125, 0.025, 0.5, and 1 mg mL−1 to obtain a standard curve for comparison with seed extracts (equation: y = 2676x − 903.3; R2: 0.982).

Irrigation assay with S. virgata seed coat extracts - Irrigation assays were performed as described above for the co-germination experiments, and seeds of the three tests species were germinated under the same experimental conditions (laboratory, greenhouse, and field), with the same experimental design. Sesbania virgata seeds were replaced by aqueous seed coat extracts at 0, 0.1, 0.5, and 1.0%, and a positive control solution of commercial 1 mg mL−1 (+)-catechin (1%). In laboratory assays, the seeds were irrigated every 48 h, during the test period, with 3‒5 mL of distilled water (control), concentrated seed coat extracts, or catechin, totaling seven irrigation treatments per species. G%, GS and GSI were determined after seven days and root length was measured at the end of the experiment (15 days). In the greenhouse and field assays, seeds and after seedlings were watered daily with 15 mL of distilled water (control), concentrated seed coat extracts, or catechin until the end of the experiment (90 days). After seven days, G%, GS and GSI of the assayed species were calculated and plant height, shoot diameter, and shoot dry weight were recorded at the end of the bioassay. Each of the three experimental conditions included four replicates per treatment with five seeds per replicate.

Experimental design and statistical analysis - Each assay was analyzed as a randomized complete block design using SISVAR 5.1 (Ferreira 2011Ferreira, D.F. 2011. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia (UFLA) 35(6): 1039-1042.). Data were analyzed using analysis of variance (ANOVA) and significant differences between treatment means were identified by the Tukey’s test (p < 0.05).

Results

Germination process - The G% of the species tested was not significantly affected by the number of S. virgata seeds in laboratory, greenhouse, or field assays (table 1). However, the GSI of M. bimucronata seeds grown in the laboratory with 10 S. virgata seeds was significantly lower (0.31) compared to controls (0.45), the opposite being observed in the data referring to the GS, where the highest values were registered with the largest number of seeds. Similarly, the GSI of P. dubium seeds grown in the greenhouse with 10 S. virgata seeds was significantly lower (0.11) compared to controls (0.15). Regarding the GS data obtained for M. bimucronata and P. dubium in greenhouse, it was observed that the greater number of seeds caused an increase in the germination speed values.

Table 1
Germination rate (G%), germination speed index (GSI) and germination speed (GS) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seeds grown with Sesbania virgata (Cav.) Pers. seeds (0, 5, and 10 seeds).

In the greenhouse irrigation assay, the G% was significantly lower in M. bimucronata seeds treated with 0.5 or 1.0% S. virgata extracts (table 2), whereas the GSI of M. bimucronata seeds treated with S. virgata extracts at all concentrations was significantly lower than controls in laboratory and greenhouse assays. In addition, the GSI of M. bimucronata seeds treated with catechin extract in greenhouse assays was similar to that of seeds treated with 0.5 or 1.0% S. virgata extracts. A significant increase in the GS variable was also observed for individuals of M. bimucronata in laboratory and greenhouse conditions, when in contact with a greater number of S. virgata seeds.

Table 2
Germination rate (G%), germination speed index (GSI) and germination speed (GS) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seeds after treatment with Sesbania virgata (Cav.) Pers. seed coat extracts (0.1, 0.5, and 1.0% w/v) or catechin (C) solution. Control (0) corresponds to treatment with distilled water.

Initial growth - Root length of M. bimucronata, P. dubium, and C. langsdorffii seedlings decreased significantly with increasing number of S. virgata seeds in laboratory germination assays (figure 1 a). The shortest root length for M. bimucronata and C. langsdorffii seedlings grown with five S. virgata seeds was 2.55 and 1.85 cm, respectively, and 2.04 cm for M. bimucronata seedlings grown with 10 S. virgata seeds. In irrigation assays, mean root lengths of M. bimucronata and P. dubium seedlings were shorter than those of control seedlings (figure 1 b). In addition, mean root length was shorter in P. dubium seedlings treated with catechin extract (2.34 cm) compared to controls (4.88 cm).

Figure 1
Mean root length (cm) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings in laboratory assays. a. co-germination assays with 0, 5 and 10 seeds. b. irrigation assays with 0.0, 0.1, 0.5, 1.0% extracts, and catechin (C). Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments are not significantly different by the Tukey’s test at p < 0.05.

Figure 1. Mean root length (cm) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaiferalangsdorffii Desf. seedlings in laboratory assays. a. co-germination assays with 0, 5 and 10 seeds. b. irrigation assays with 0.0, 0.1, 0.5, 1.0% extracts, and catechin (C). Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments are not significantly different by the Tukey's test at p < 0.05.

In greenhouse assays, plant height was significantly shorter in M. bimucronata seedlings grown with S. virgata seeds, regardless of the number of seeds (figure 2 a). The presence of S. virgata seeds negatively affected shoot diameter in M. bimucronata and C. langsdorffii seedlings (figure 2 b). Mean shoot dry weight of M. bimucronata seedlings was negatively and significantly affected by the presence of S. virgata seeds in greenhouse germination assays, whereas no effect was detected in P. dubium and C. langsdorffii seedlings (figure 2 c).

Figure 2
Mean plant height (cm), shoot diameter (mm) and shoot dry weight (g) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings grown with Sesbania virgata (Cav.) Pers. seeds in greenhouse co-germination assays. a. plant height. b. shoot diameter. c. shoot dry weight. Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments (0, 5, and 10 seeds) are not significantly different by the Tukey’ test at p < 0.05.

Figure 2. Mean plant height (cm), shoot diameter (mm) and shoot dry weight (g) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings grown with Sesbania virgata (Cav.) Pers. seeds in greenhouse co-germination assays. a. plant height. b. shoot diameter. c. shoot dry weight. Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments (0, 5, and 10 seeds) are not significantly different by the Tukey' test at p < 0.05.

In the field assay, mean plant height of C. langsdorffii seedlings grown with five and 10 S. virgata seeds was significantly smaller (3.65 and 3.3 cm, respectively) (figure 3 a). Mean shoot diameter of seedlings grown in the field was not significantly affected by with the presence of S. virgata seeds (figure 3 b). The more gradual reduction in shoot dry weight was observed in P. dubium and C. langsdorffii seedlings grown with five and 10 S. virgata seeds (figure 3 c)

Figure 3
Mean plant height (cm), shoot diameter (mm) and shoot dry weight (g) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings grown with Sesbania virgata (Cav.) Pers. seeds in field co-germination assays. a. plant height. b. shoot diameter. c. shoot dry weight. Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments (0, 5, and 10 seeds) are not significantly different by the Tukey’s test at p < 0.05.

Figure 3. Mean plant height (cm), shoot diameter (mm) and shoot dry weight (g) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings grown with Sesbania virgata (Cav.) Pers. seeds in field co-germination assays. a. plant height. b. shoot diameter. c. shoot dry weight. Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments (0, 5, and 10 seeds) are not significantly different by the Tukey's test at p < 0.05.

Plant height of M. bimucronata, P. dubium, and C. langsdorffii seedlings was significantly affected in greenhouse irrigation experiments (figure 4 a). Plant height of M. bimucronata and P. dubium seedlings treated with 0.5% S. virgata extract was significantly smaller compared to control seedlings, whereas 0.1 and 1.0% extracts had the strongest negative effect on plant height in C. langsdorffii seedlings (7.23 and 6.78 cm, respectively). In addition, mean shoot diameter of P. dubium and C. langsdorffii species in the greenhouse assay was significantly smaller in plants irrigated with either S. virgata seed extracts or catechin (figure 4 b). For M. bimucronata, only 0.1% extracts did not induce reductive effects in relation to the control treatment. Mean shoot dry weight was significantly smaller in P. dubium and C. langsdorffii seedlings treated with S. virgata seed extracts, and the 0.5% extract had the strongest effect on P. dubium (figure 4 c).

Figure 4
Mean plant height (cm), shoot diameter (mm) and shoot dry weight (g) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings treated with Sesbania virgata (Cav.) Pers. seed extracts and catechin in greenhouse irrigation assays. a. plant height. b. shoot diameter. c. shoot dry weight. Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments (0.0, 0.1, 0.5, 1.0% extracts, and catechin [C]) are not significantly different by the Tukey’s test at p < 0.05.

Figure 4. Mean plant height (cm), shoot diameter (mm) and shoot dry weight (g) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings treated with Sesbania virgata (Cav.) Pers. seed extracts and catechin in greenhouse irrigation assays. a. plant height. b. shoot diameter. c. shoot dry weight. Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments (0.0, 0.1, 0.5, 1.0% extracts, and catechin [C]) are not significantly different by the Tukey's test at p < 0.05.

In field assays, mean plant height and shoot diameter of plants treated with S. virgata seed extracts were significantly smaller only in C. langsdorffii plants and the 1.0% extract caused the most significant effect both for plant height (3.57 cm, figure 5 a) and shoot diameter (1.01 mm, figure 5 b). Mean shoot dry weight was significantly smaller only in C. langsdorffii with the greatest reductions observed in plants treated with 0.5 and 1.0% S. virgata seed extracts (0.11 and 0.09 g, respectively, figure 5 c).

Figure 5
Mean (a) plant height (cm), (b) shoot diameter (mm) and (c) shoot dry weight (g) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings treated with Sesbania virgata (Cav.) Pers. seed extracts and catechin in field irrigation assays. a. plant height. b. shoot diameter. c. shoot dry weight. Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments (0.0, 0.1, 0.5, 1.0% extracts, and catechin [C]) are not significantly different by the Tukey’s test at p < 0.05.

Figure 5. Mean (a) plant height (cm), (b) shoot diameter (mm) and (c) shoot dry weight (g) of Mimosa bimucronata (DC.) Kuntze, Peltophorum dubium (Spreng.) Taub. and Copaifera langsdorffii Desf. seedlings treated with Sesbania virgata (Cav.) Pers. seed extracts and catechin in field irrigation assays. a. plant height. b. shoot diameter. c. shoot dry weight. Bars are means (±SD) of four replicates. Means followed by the same letter for each species and between treatments (0.0, 0.1, 0.5, 1.0% extracts, and catechin [C]) are not significantly different by the Tukey's test at p < 0.05.

Catechin quantification - The exudation profile of S. virgata seeds by HPLC during a 48 h imbibition period showed that peak catechin release was achieved at 18 h, followed by a gradual decrease in catechin concentration (figure 6).

Figure 6
Catechin quantification (λ = 280 nm) in Sesbania virgata (Cav.) Pers. seed exudates by HPLC. Seeds were imbibed in distilled water and collected at six-hour intervals. Vertical bars represent the standard deviation of the mean (n = 3).

Figure 6. Catechin quantification (λ = 280 nm) in Sesbania virgata (Cav.) Pers. seed exudates by HPLC. Seeds were imbibed in distilled water and collected at six-hour intervals. Vertical bars represent the standard deviation of the mean (n = 3).

Discussion

Seeds germination - Sesbania virgata has been widely employed in restoration programs (Zanandrea et al. 2009Zanandrea, I., Alves, J.D., Deuner, S., Goulart, P.F.P., Henrique, P.C. & Silveira, N.M. 2009. Tolerance of Sesbania virgata plants to flooding. Australian Jornal of Botany 57: 661-669., Florentino et al. 2009Florentino, L.A., Guimarães, A.P., Rufini, M., da Silva, K. & Moreira, F.M.S. 2009. Sesbania virgata stimulates the occurrence of its microsymbiont in soils but does not inhibit microsymbionts of other species. Scientia Agricola 66: 667-676., Branzini et al. 2012Branzini, A., González, R.S. & Zubillaga, M. 2012. Absorption and translocation of copper, zinc and chromium by Sesbania virgata. Journal of environmental management 102: 50-54.). However, S. virgata is considered a superdominant species, which is able to outcompete other plant species by inhibiting their germination or development (Mignoni et al. 2017Mignoni, D.S.B., Simões, K. & Braga, M.R. 2017. Potential allelopathic effects of the tropical legume Sesbania virgata on the alien Leucaena leucocephala related to seed carbohydrate metabolism. Biological Invasions: 1-16., El Id et al. 2015El Id, V.L., Costa, B.V., Mignoni, D.S.B., Veronesi, M.B., Simões, K., Braga, M.R. & Santos Junior, N.A. 2015. Phytotoxic effect of Sesbania virgata (Cav.) Pers. on seeds of agronomic and forestry species. Journal of Forestry Research 26: 339-346.).

During seed imbibition, allelopathic compounds capable of inhibiting or retarding cell multiplication or growth may penetrate the seeds and delay germination (Bhadoria 2011Bhadoria, P.B.S. 2011. Allelopathy: a natural way towards weed management. American Journal of Experimental Agriculture 1(1): 7-20. ). For instance, the presence of S. virgata seeds was shown to inhibit the germination of agronomic species (El Id et al. 2015El Id, V.L., Costa, B.V., Mignoni, D.S.B., Veronesi, M.B., Simões, K., Braga, M.R. & Santos Junior, N.A. 2015. Phytotoxic effect of Sesbania virgata (Cav.) Pers. on seeds of agronomic and forestry species. Journal of Forestry Research 26: 339-346.). Similarly, Mignoni et al. (2017)Mignoni, D.S.B., Simões, K. & Braga, M.R. 2017. Potential allelopathic effects of the tropical legume Sesbania virgata on the alien Leucaena leucocephala related to seed carbohydrate metabolism. Biological Invasions: 1-16. found that substances exuded by the seeds of S. virgata negatively affected the G% and GSI of Leucaena leucocephala (Lam.) de Wit. in germination experiments.

Even though M. bimucronata and P. dubium are pioneer species and may coexist with S. virgata, the lower GSI of M. bimucronata compared to that of P. dubium indicates that the former is more sensitive than the latter and the species studied by El Id et al. (2015)El Id, V.L., Costa, B.V., Mignoni, D.S.B., Veronesi, M.B., Simões, K., Braga, M.R. & Santos Junior, N.A. 2015. Phytotoxic effect of Sesbania virgata (Cav.) Pers. on seeds of agronomic and forestry species. Journal of Forestry Research 26: 339-346. and Mignoni et al. (2017)Mignoni, D.S.B., Simões, K. & Braga, M.R. 2017. Potential allelopathic effects of the tropical legume Sesbania virgata on the alien Leucaena leucocephala related to seed carbohydrate metabolism. Biological Invasions: 1-16. to the phytochemicals released by S. virgata. A similar effect was observed in irrigation assays, in which S. virgata seed extracts were particularly effective against M. bimucronata, as indicated by the reduction in G% in laboratory test, by the increase in GS data in laboratory and greenhouse and GSI, in laboratory and greenhouse tests. The more significant effect of S. virgata on the GS and GSI of M. bimucronata and the lack of significant changes in the germination process of P. dubium and C. langsdorffii indicate that one or more stages of the germination process or mechanisms other than germination can be affected by compounds released from S. virgata seeds.

The lack of a significant effect on the GS and GSI of M. bimucronata, P. dubium, and C. langsdorffii under field conditions can be explained by the interference of environmental factors such as rainfall or microbial degradation in the soil on the action of allelopathic compounds (Trezzi et al. 2016Trezzi, M.M., Vidal, R.A., Balbinot Junior, A.A., von Hertwig Bittencourt, H. & Silva Souza Filho, A.P. 2016. Allelopathy: driving mechanisms governing its activity in agriculture. Journal of Plant Interactions 11(1): 53-60.). Moreover, forest species are not always sensitive to these substances. It should also be noted that plant resistance or tolerance to secondary metabolites is believed to be an intrinsic trait of the species mediated by environmental conditions (Inderjit & Duke 2003Inderjit & Duke, S.O. 2003. Ecophysiological aspects of allelopathy. Planta 217(4): 529-539., Inderjit et al. 2011Inderjit, Wardle, D.A., Karban, R. & Callaway, R.M. 2011. The ecosystem and evolutionary contexts of allelopathy. Trends in ecology & evolution 26(12): 655-662.), which may explain the lack of negative effects on the germination of the species examined in this study.

Our results indicate that the phytochemicals released by S. virgata do not target seed germination in the species tested and provide the basis for future investigation of other species that use allelochemicals as part of their ecology. Future studies should target the mechanisms of action of the allelochemicals produced by S. virgata seeds, which provide the species with a competitive advantage in natural habitats.

Initial growth - Seedling growth rather than germination may be more sensitive to allelochemicals. According to Rice (1979)Rice EL (1979). Allelopathy. An update. Botanical Review 45: 15-109., when seeds of sensitive species are exposed to allelochemicals, germination may be inhibited and their seedlings may also be subsequently affected. For instance, Mignoni et al. (2017)Mignoni, D.S.B., Simões, K. & Braga, M.R. 2017. Potential allelopathic effects of the tropical legume Sesbania virgata on the alien Leucaena leucocephala related to seed carbohydrate metabolism. Biological Invasions: 1-16. showed that L. leucocephala seedlings developed a shortened, darkened hypocotyl in seedling roots when grown with S. virgata seeds, indicating that compounds released by S. virgata seeds can inhibit the growth of different organs in some plant species. A similar effect was observed in the current study, as indicated by the reduction in root length, seedling height, shoot diameter, and shoot dry weight of plants grown with S. virgata seeds or irrigated with its extract.

Data for (+)-catechin release by S. virgata seeds are shown in figure 8. Peak (+)-catechin release was achieved up to 18 h after the onset of imbibition, confirming the findings of Simões et al. (2008)Simões, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.R., Vivanco, J.M. & Braga, M.R. 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology 34: 681-687.. Interestingly, radicle protrusion in M. bimucronata occurs up to 24 h after the start of imbibition (data not shown). Because this species was one of the most affected in both laboratory and greenhouse assays, it is likely to have come into greater contact with compounds exuded by S. virgata compared to P. dubium and C. langsdorffii.

The initial development of P. dubium was less affected by compounds released by S. virgata seeds compared to that of M. bimucronata and C. langsdorffii in germination tests in the greenhouse and field. The weak effect of S. virgata compounds on P. dubium seeds may be related to the presence of galactomannan in the latter endosperm cell walls, which acts as a buffering physical barrier that controls the entry of water and other substances into the seed (Tonini et al. 2006Tonini, P.P., Lisboa, C.G.S., Freschi, L., Mercier, H., Mazzoni-Viveiros, S.C. & Buckeridge, M.S. 2006. Effect of abscisic acid on galactomannan degradation and endo-β-mannanase activity in seeds of Sesbania virgata (Cav.) Pers. (Leguminosae). Trees 20(6): 669-678., Buckeridge 2010Buckeridge, M.S. 2010. Seed cell wall storage polysaccharides: models to understand cell wall biosynthesis and degradation. Plant Physiology 154: 1017-1023.). Thus, the galactomannan stored in the endosperm could also regulate the entry of allelochemicals released by S. virgata, preventing the initial contact of the P. dubium embryo with these substances in the first 24 h of imbibition.

After the degradation of galactomannan, the P. dubium embryo may have come into contact with the substances exuded by S. virgata seeds, possibly causing the adverse effects observed on root length and shoot dry weight. Thus, plant development was negatively affected in P. dubium seeds irrigated with S. virgata seed extracts, resulting in reduced seedling root length, height, shoot diameter, and shoot dry weight. In addition, this finding suggests that compounds other than catechin with allelopathic potential in the tegument of S. virgata seeds are not released during the first stages of imbibition, because plant development was more affected by application of seed extracts than germination with S. virgata seeds.

These allelochemicals may have been released later and been able to inhibit the development of M. bimucronata and C. langsdorffii. In fact, the alkaloid ‘sesbanimida A’ has high phytotoxic potential, is capable of inhibiting the growth of other plant species, and has been detected in S. virgata seeds (van Staden & Grobbelaar 1995van Staden, J. & Grobbelaar, N. 1995. The effect of sesbanimide and Sesbania seed extracts on germination and seddling growth of a number of plant species. Environmental and Experimental Botany 35: 321-329., Simões et al. 2008Simões, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.R., Vivanco, J.M. & Braga, M.R. 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology 34: 681-687.). Additionally, Simões et al. (2008)Simões, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.R., Vivanco, J.M. & Braga, M.R. 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology 34: 681-687. reported the presence of the flavonoid ‘quercetin’ in the tegument of S. virgata. These compounds are toxic and may have adversely affected some parameters related to the development of M. bimucronata and C. langsdorffii seedlings.

The initial growth of C. langsdorffii seedlings was significantly reduced in nearly all treatments under nearly all conditions. The onset of C. langsdorffii germination occurred between days 18 and 21 in the laboratory and 26 and 30 in the greenhouse and field. In the field, this species was sensitive to commercial catechin, aqueous S. virgata extracts, and S. virgata seeds. This result suggests S. virgata seeds have compounds with inhibitory potential, which may have persisted in the environment during the bioassay period. According to Del Fabbro et al. (2014)Del Fabbro, C., Güsewell, S. & Prati, D. 2014. Allelopathic effects of three plant invaders on germination of native species: a field study. Biological invasions 16(5): 1035-1042., allelopathic compounds can persist in soils. Phytochemicals can combine in the soil in various ways and may strongly affect the metabolism of other plants (Inderjit et al. 2011Inderjit, Wardle, D.A., Karban, R. & Callaway, R.M. 2011. The ecosystem and evolutionary contexts of allelopathy. Trends in ecology & evolution 26(12): 655-662.).

Catechin has allelopathic properties and is found in S. virgata seeds (Simões et al. 2008Simões, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.R., Vivanco, J.M. & Braga, M.R. 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology 34: 681-687.). The catechin molecule contains reactive hydroxyl groups, which can be oxidized by free radicals. Thus, flavonoids may act directly sequestering free radicals, resulting in a more stable chemical structure (Nijveldt et al. 2001Nijveldt, R.J., van Nood, E., van Hoorn, D.E.C., Boelens, P.G., van Norren, K., van Leeuwen, P.A.M. 2001. Flavonoids: a review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition 74: 418-425.). When catechins occur in a chemically uncontrolled condition, they may undergo changes that result in the loss of their inhibitory potential. Moreover, it has been argued that this molecule has a short half-life in the soil, rapidly degrading and losing its phytotoxicity (Inderjit et al. 2008Inderjit, Pollock, J.L., Callaway, R.M. & Holben, W. 2008. Phytotoxic effects of (±)- catechin in vitro, in soil, and in the field. Plos One 3: 1-11., Duke et al. 2009Duke, S.O., Dayan, F.E., Bajsa, J., Meepagala, K.M., Hufbauer, R.A. & Blair, A.C. 2009. The case against (-) -catechin involvement in allelopathy of Centaurea stoebe (spotted knapweed). Plant signaling & behavior 4(5): 422-424.). Thus, the intensity of catechin effects not only varies among species, but also depends on the experimental or other prevailing conditions (Buta & Lusby 1986Buta, J.G. & Lusby, W.R. 1986. Catechins as germination and growth inhibitors in Lespedeza seeds. Phytochemistry 25: 93-95., Weir et al. 2006Weir, T.L., Bais, H.P., Stull, V.J., Callaway, R.M., Thelen, G.C., Ridenour, W.M., Bhamidi, S., Stermitz, F.R. & Vivanco, J.M. 2006. Oxalate contributes to the resistance of Gaillardia grandflora and Lupinus sericeus to a phytotoxin produced by Centaurea maculosa. Planta 223, 785-795.). In the current study, the intensity of catechin effects was different in the field and the laboratory, because laboratory conditions are usually more stringently controlled and it is possible to eliminate external factors that may affect the experiment results.

The catechin molecule is known to be more unstable in soils with alkaline pH (Blair et al. 2005Blair, A.C., Hanson, B.D., Brunk, G.R., Marrs, R.A., Westra, P., Nissen, S.J. & Hufbauer, R.A. 2005. New techniques and findings in the study of a candidate allelochemical implicated in invasion success. Ecology Letters 8(10): 1039-1047., Furubayashi et al. 2007Furubayashi, A., Hiradate, S., Fujii, Y. 2007. Role of catechol structure in the adsorption and transformation reactions of l-D opa in soils. Journal of chemical ecology 33(2): 239-250. ) and to have greater toxicity in soils with higher organic matter content (Inderjit et al. 2008Inderjit, Pollock, J.L., Callaway, R.M. & Holben, W. 2008. Phytotoxic effects of (±)- catechin in vitro, in soil, and in the field. Plos One 3: 1-11.). Brazilian soils are mostly acidic and field trials in the current study were conducted near a forest fragment in an area with a large supply of organic matter. Thus, despite its short half-life in the soil, catechin may have had a negative effect on plant development, as shown by the significant reduction in growth parameters for plants irrigated with catechin extracts, especially in field assays. At the same concentration (1 mg mL−1), the effect of irrigation with catechin was weaker compared to aqueous extracts from the tegument of S. virgata seeds. This result also suggests that, in addition to catechin, S. virgata seed extracts contain other compounds with inhibitory potential.

In conclusion, the lack of effect of seed extracts on the germination of native plant species suggests that the allelochemicals produced by S. virgata seeds act mainly during the post-germination stage rather than during seed germination. Seedling development may have been inhibited by the presence of catechin or other compounds in the tegument. It is possible that these compounds remain in the environment for some time, affecting the development of the species tested. In addition, M. bimucronata and C. langsdorffii seedlings were more sensitive to the allelochemicals released by S. virgata during their initial development than P. dubium. This result suggests that resistance to allelochemicals is not linked to the successional stage of a species but is determined by intrinsic characteristics that provide the capacity to tolerate the phytotoxins released by S. virgata seeds. The high competitive ability of S. virgata across habitat types against several plant species can be explained by the production of these phytotoxins, together with its high capacity for soil cover, seed bank formation, and affinity for modified soils.

  • 1
    Part of the Master’s Dissertation of the first Author

Acknowledgements

This work is part of the Part of the Master’s Dissertation of V.L. El Id at Instituto de Botânica, SP (Brazil) and was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Thanks are due to CNPq, to Research Center in Ornamental Plants, for the infrastructure and HPLC and to Dr. Rodrigo Sant’Ana Cabral, for the help with catechin quantification.

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Publication Dates

  • Publication in this collection
    20 Nov 2020
  • Date of issue
    2020

History

  • Received
    08 May 2020
  • Accepted
    01 Sept 2020
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