Etiology and pathogenicity of two different isolates of Colletotrichum spp. obtained from physic nut seeds

Santos, Gil Rodrigues dos; Tozze Júnior, Hugo José; Sá, Danila Alves Corrêa de; Furtado, Gleiber Quintão; Massola Júnior, Nelson Sidnei

Abstracts

The species known as physic nut (Jatropha curcas L.) has become important as one of main sources of feedstock for biodiesel production. The aims of this study were characterizing two different isolates of Colletotrichum spp. obtained from seeds of this species, through morphological, cultural, and molecular analyses; as well as assessing pathogenicity of both isolates on leaves and fruit of this plant species. For morphological analysis, length and width of 30 spores of each isolate, produced on medium Malt Extract-Agar (MEA), at 25 ± 1 ºC, and under constant light, were measured. Cultural analysis was performed by the fungus growth on PDA medium, through daily measurements of the mycelial growth, and the color of colonies after seven days incubation. DNA of the isolates was extracted and specific oligonucleotides primers (region ITS4) were identified by PCR and used to identify C. capsici (CcInt) and C. gloeosporioides (CgInt). Pathogenicity of isolates was assessed on plants aged 10, 20, 30, 40, 50, and 60 days after sowing and fruits at six maturation stages. Based on these analyses, isolates were identified as C. gloeosporioides and C. capsici. Young plants and the older fruits were more susceptible to infection by the two fungus species.

anthracnose; Jatropha curcas; C. capsici; C. gloeosporioides

A espécie pinhão manso (Jatropha curcas L.), vem se destacando como a principal matéria-prima para produção de biodiesel. O objetivo deste estudo foi caracterizar dois isolados de Colletotrichum spp., obtidos de sementes dessa espécie, através de análise morfológica, cultural e molecular, além de avaliar a patogenicidade desses isolados em folhas e frutos da planta. Para análise morfológica foram medidos o comprimento e a largura de 30 esporos de cada isolado, produzidos em meio de cultura Extrato de Malte-Agar, a 25±1 ºC, sob luz contínua. A análise cultural foi realizada pelo crescimento do fungo em meio de BDA, com avaliações diárias do crescimento do micélio e pela coloração da colônia, após sete dias de incubação. O DNA dos isolados foi extraído e oligonucleotídeos específicos (região ITS4) foram identificados pela técnica de PCR e utilizados para identificar C. capsici (CcInt) e C. gloeosporioides (CgInt). A patogenicidade dos isolados foi avaliada em plantas com diferentes idades (10, 20, 30, 40, 50 e 60 dias após semeadura) e em frutos em seis estádios de maturação. Com base nestas análises os isolados foram identificados como C. capsici e C. gloeosporioides. Plantas jovens e frutos desenvolvidos foram mais suscetíveis à infecção pelas duas espécies do fungo.

antracnose; Jatropha curcas; C. capsici; C. gloeosporioides

AVRDC. 2004. Evaluation of phenotypic and molecular criteria for the identification of Colletotrichum species causing pepper anthracnose in Taiwan. Progress Report, 2003. Shanhua: AVRDC. http://www.avrdc.org/pdf/pr2003.pdf. Accessed on: 01^st May 2011.
BAILEY, L.A; JEGGER, M.J. Colletotrichum: Biology, Pathology and control Ed. Wallingford: CAB International, 1992. 388p.
BINYAMINI, N.; SCHIFFMANN-NADEL, M. Latent infection in avocado fruit due to Colletotrichum gloeosporioides Phytopathology, v.62, p.592-594, 1972.
CAI, L.; HYDE, K.D.; TAYLOR, P.W.J.; WEIR, B.S.; WALLER, J.; ABANG, M.M.; ZHANG, J.Z.; YANG, Y.L.; PHOULIVONG, S.; LIU, Z.Y.; PRIHASTUTI, H.; SHIVAS, R.G.; MCKENZIE, E.H.C.; JOHNSTON, P.R. A polyphasic approach for studying Colletotrichum Fungal Diversity, v.39, p.183-204, 2009. http://www.fungaldiversity.org/fdp/sfdp/fd39-8.pdf
CHILLET, M.; HUBERT, O; DE BELLAIRE, L.L. Relationship between physiological age, ripening and susceptibility of banana to wound anthracnose. Crop Protection, v.26, n.7, p.1078-1082, 2007. http://www.sciencedirect.com/science/article/pii/s0261219406002894
DAMM, U.; BARONCELLI, R.; CAI, L.; KUBO, Y.; O'CONNELL, R.; WEIR, B.; YOSHINO, K.; CANNON, P. F. Colletotrichum: species, ecology and interactions. IMA FUNGUS, v.1, n.2, p. 161 - 165, 2010. http://www.imafungus.org/Issue/2/19.pdf
DELLAPORTA, S.L; WOOD, J.; HICKS, J.B. A plant DNA mini-preparation: version II. Plant Molecular Biology Reporter, v.1, n.4, p.19-21, 1983. http://www.springerlink.com/content/g028p2457t792qn4/
DICKINSON, C.H.; CRUTE, J.R. The influence of seedling age and development on the infection of lettuce by Bremia lactucae Annual Applied of Biology, v.76, n.1, p.49-61, 1974. HTTP://ONLINELIBRARY.WILEY.COM/DOI/10.1111/J.1744-7348.1974.TB01356.X/ABSTRACT
FERREIRA, D.F.; SISVAR: Sistema de análise de variância. Versão 4.6. Lavras: UFLA/DEX, 2003.
FRANCO, D.A.S.; GABRIEL, D. Aspectos fitossanitários na cultura do pinhão manso (Jatropha curcas L.) para produção de biodiesel. Biológico, v.70, n.2, p.63-64, 2008.
GOMEZ, K.A.; GOMEZ, A.A. Statistical procedures for agricultural research. 2ed. New York: John Wiley, 1984. 680P.
HONG, J.K.; LEE, Y.K.; JEUN, Y.C.; HWANG, B.K. Histological and ultrastructural study of susceptible and age-related resistance responses of pepper leaves to Colletotrichum coccodes infection. The Plant Pathology Journal, v.17, p.128-140, 2001.
HYDE, K.D.; CAI, L.; CANNON, P.F.; CROUCH, J.A.; CROUS, P.W.; DAMM, U.; GOODWIN, P.H.; CHEN, H.; JOHNSTON, P.R.; JONES, E.B.G.; LIU, Z.Y.; MCKENZIE, E.H.C.; MORIWAKI, J.; NOIREUNG, P.; PENNYCOOK, S.R.; PFENNING, L.H.; PRIHASTUTI, H.; SATO, T.; SHIVAS, R.G.; TAN, Y.P.; TAYLOS, P.W.J.; WEIR, B.S.; YANG, Y.L.; ZHANG, J.Z. Colletotrichum - names in current use. Fungal Diversity, v. 39, p. 147-182, 2009. http://www.fungaldiversity.org/fdp/sfdp/fd39-7.pdf
KUMAR, A.; SHARMA, S. An evaluation of multipurpose oil seed crop for industrial uses (Jatropha curcas L.): A review. Industrial Crops and Products, v.28, p.1-10, 2008. http://www.sciencedirect.com/science/article/pii/s0926669008000046
LOPEZ, A.M.Q. Taxonomia, patogênese e controle de espécies do gênero Colletotrichum Revisão anual de patologia de plantas, v.9, p.291-337, 2001.
MAUCH, F.; STAEHELIN, L.A. Functional implications of the subcellular localization of ethylene-induced chitinase and β-1,3-glucanase in bean leaves. The Plant Cell, v.1, p.447-457, 1989. http://www.ncbi.nlm.nih.gov/pmc/articles/pmc159776/pdf/010447.pdf
MILLS, P.R.; SREENIVASAPRASAD, S.; BROWN, A.E. Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR. FEMS Microbiology Letters, v.98, p.137-144, 1992.
MORAL, J.; BOUHMIDI, K.; TRAPERO, A. Influence of fruit maturity, cultivar susceptibility, and inoculation method on infection of olive fruit by Colletotrichum acutatum Plant Disease, v.92, n.10, p.1421-1426, 2008. http://apsjournals.apsnet.org/doi/pdfplus/10.1094/pdis-92-10-1421
OPENSHAW, K. A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass and Bioenergy, v.19, n.1, p.1-15, 2000. http://pdn.sciencedirect.com/science?_ob=miamiimageurl&_cid=271477&_user=687369&_pii= s0961953400000192&_ check=y&_origin=browse&_zone=rslt_list_item&_coverdate=2000-07-01&wchp=dglbvlk-zskwz&md5=f993cf141b7616fa70fb0988a361f432/1-s2.0-s0961953400000192-main
PEGG, K.G.; COATES, L.M.; KORSTEN, L.; HARDING, R.M. Foliar, fruit and soil diseases. In: WHILEY, A.W.; SCHAFFER, B.; WOLSTENHOLME, B.N. (Ed.). The avocado: botany, production and uses. Wallingford: CAB International Press, 2002. p.299-338.
PEREIRA, O.L.; DUTRA, D.C.; DIAS, L.A.S. Lasiodiplodia theobromae is the causal agent of a damaging root and collar rot disease on the biofuel plant Jatropha curcas in Brazil. Australasian Plant Disease Notes, v.4, n.1, p.120-123, 2009. http://www.publish.csiro.au/?act=view_file&file_id=dn09049.pdf
PRING, R.J.; NASH, C.; ZAKARIA, M.; BAILEY, J.A. Infection processes and host range of Colletotrichum capsici. Physiological and Molecular Plant Pathology, v.46, n.2, p.137-152, 1995. http://www.dfid.gov.uk/r4d/output/12324/default.aspx
PRUSKY, D. Pathogen quiescence in postharvest disease. Annual Review of Phytopathology, v.34, p.413-434, 1996. http://www.annualreviews.org/doi/pdf/10.1146/annurev.phyto.34.1.413
PRUSKY, D.; KEEN, N.T. Involvement of preformed antifungal compounds in the resistance of subtropical fruits to fungal decay. Plant Disease, v.77, n.1, p.114-117, 1993. http://www.apsnet.org/publications/plantdisease/backissues/documents/1993articles/ plantdisease77n02_114.pdf
RAJ, S.K.; SNEHI, S.K.; KUMAR, S.; KHAN, M.S.; PATHRE, U. First molecular identification of a begomovirus in India that is closely related to Cassava mosaic virus and causes mosaic and stunting of Jatropha curcas L. Australasian Plant Disease Notes, v.3, p.69-72, 2008. http://www.springerlink.com/content/u76424vwh6411050/fulltext.pdf
REUVENI, M.; TUZUNS, S.; COLE, J.S.; SIEGEL, M.R.; KUÉ, J. The effects of plant age position on the susceptibility of tobacco to blue mold caused by Peronospora tabacina Phytopathology, v.76, n.4, p.455-458, 1986. http://www.apsnet.org/publications/phytopathology/backissues/documents/1986articles/ phyto76n04_455.pdf
SÁ, D.A.C.; SANTOS, G.R.; FURTADO, G.Q.; LEMUS, E.A.; NASCIMENTO, I.R. Transporte, patogenicidade e transmissibilidade de fungos associados às sementes de pinhão manso. Revista Brasileira de Sementes, v.33, n.4, p.663-670, 2011. http://www.scielo.br/pdf/rbs/v33n4/08.pdf
SANTOS, G.R.; CASTRO NETO, M.D.; RAMOS, L.N.; CAFÉ-FILHO, A.C.; REIS, A.; MOMENTÉ, V.G., PELÚZIO, J.M.; IGNÁCIO, M. Reaction of melon genotypes to the gummy stem blight and the downy mildew. Horticultura Brasileira, v.27, n.2, p.160-165, 2009. http://www.scielo.br/pdf/hb/v27n2/v27n2a07.pdf
SMITH, B.; BLACK, L.L.; GALLETTA, G.J. Resistance to Colletotrichum fragariae strawberry affected by seedling age and inoculated method. Plant Disease, v.74, n.12, p.1016-1021, 1990. http://www.apsnet.org/publications/plantdisease/backissues/documents /1990articles/plantdisease74n12_1016.pdf
SUTTON, B.C. The genus Glomerella and its anamorph Colletotrichum In: Bailey, J.A.; & Jeger, M.J. (Eds.) Colletotrichum - Biology, Pathology and Control Wallingford: CAB International, 1992. p.1-26.
TANAKA, M.A.S.; PASSOS, F.A.; ITO, M.F. Influência da cultivar e do estádio fenológico do fruto de morangueiro sob o desenvolvimento de lesões causadas por Colletotrichum spp. Summa Phytopathologica, v.20, n.3, p.160-163, 1994.

Publication Dates

Publication in this collection
24 June 2013
Date of issue
2013

History

Received
16 Feb 2012
Accepted
23 Oct 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] AVRDC. 2004. Evaluation of phenotypic and molecular criteria for the identification of Colletotrichum species causing pepper anthracnose in Taiwan. Progress Report, 2003. Shanhua: AVRDC. http://www.avrdc.org/pdf/pr2003.pdf. Accessed on: 01^st May 2011.

[2] BAILEY, L.A; JEGGER, M.J. Colletotrichum: Biology, Pathology and control Ed. Wallingford: CAB International, 1992. 388p.

[3] BINYAMINI, N.; SCHIFFMANN-NADEL, M. Latent infection in avocado fruit due to Colletotrichum gloeosporioides Phytopathology, v.62, p.592-594, 1972.

[4] CAI, L.; HYDE, K.D.; TAYLOR, P.W.J.; WEIR, B.S.; WALLER, J.; ABANG, M.M.; ZHANG, J.Z.; YANG, Y.L.; PHOULIVONG, S.; LIU, Z.Y.; PRIHASTUTI, H.; SHIVAS, R.G.; MCKENZIE, E.H.C.; JOHNSTON, P.R. A polyphasic approach for studying Colletotrichum Fungal Diversity, v.39, p.183-204, 2009. http://www.fungaldiversity.org/fdp/sfdp/fd39-8.pdf

[5] CHILLET, M.; HUBERT, O; DE BELLAIRE, L.L. Relationship between physiological age, ripening and susceptibility of banana to wound anthracnose. Crop Protection, v.26, n.7, p.1078-1082, 2007. http://www.sciencedirect.com/science/article/pii/s0261219406002894

[6] DAMM, U.; BARONCELLI, R.; CAI, L.; KUBO, Y.; O'CONNELL, R.; WEIR, B.; YOSHINO, K.; CANNON, P. F. Colletotrichum: species, ecology and interactions. IMA FUNGUS, v.1, n.2, p. 161 - 165, 2010. http://www.imafungus.org/Issue/2/19.pdf

[7] DELLAPORTA, S.L; WOOD, J.; HICKS, J.B. A plant DNA mini-preparation: version II. Plant Molecular Biology Reporter, v.1, n.4, p.19-21, 1983. http://www.springerlink.com/content/g028p2457t792qn4/

[8] DICKINSON, C.H.; CRUTE, J.R. The influence of seedling age and development on the infection of lettuce by Bremia lactucae Annual Applied of Biology, v.76, n.1, p.49-61, 1974. HTTP://ONLINELIBRARY.WILEY.COM/DOI/10.1111/J.1744-7348.1974.TB01356.X/ABSTRACT

[9] FERREIRA, D.F.; SISVAR: Sistema de análise de variância. Versão 4.6. Lavras: UFLA/DEX, 2003.

[10] FRANCO, D.A.S.; GABRIEL, D. Aspectos fitossanitários na cultura do pinhão manso (Jatropha curcas L.) para produção de biodiesel. Biológico, v.70, n.2, p.63-64, 2008.

[11] GOMEZ, K.A.; GOMEZ, A.A. Statistical procedures for agricultural research. 2ed. New York: John Wiley, 1984. 680P.

[12] HONG, J.K.; LEE, Y.K.; JEUN, Y.C.; HWANG, B.K. Histological and ultrastructural study of susceptible and age-related resistance responses of pepper leaves to Colletotrichum coccodes infection. The Plant Pathology Journal, v.17, p.128-140, 2001.

[13] HYDE, K.D.; CAI, L.; CANNON, P.F.; CROUCH, J.A.; CROUS, P.W.; DAMM, U.; GOODWIN, P.H.; CHEN, H.; JOHNSTON, P.R.; JONES, E.B.G.; LIU, Z.Y.; MCKENZIE, E.H.C.; MORIWAKI, J.; NOIREUNG, P.; PENNYCOOK, S.R.; PFENNING, L.H.; PRIHASTUTI, H.; SATO, T.; SHIVAS, R.G.; TAN, Y.P.; TAYLOS, P.W.J.; WEIR, B.S.; YANG, Y.L.; ZHANG, J.Z. Colletotrichum - names in current use. Fungal Diversity, v. 39, p. 147-182, 2009. http://www.fungaldiversity.org/fdp/sfdp/fd39-7.pdf

[14] KUMAR, A.; SHARMA, S. An evaluation of multipurpose oil seed crop for industrial uses (Jatropha curcas L.): A review. Industrial Crops and Products, v.28, p.1-10, 2008. http://www.sciencedirect.com/science/article/pii/s0926669008000046

[15] LOPEZ, A.M.Q. Taxonomia, patogênese e controle de espécies do gênero Colletotrichum Revisão anual de patologia de plantas, v.9, p.291-337, 2001.

[16] MAUCH, F.; STAEHELIN, L.A. Functional implications of the subcellular localization of ethylene-induced chitinase and β-1,3-glucanase in bean leaves. The Plant Cell, v.1, p.447-457, 1989. http://www.ncbi.nlm.nih.gov/pmc/articles/pmc159776/pdf/010447.pdf

[17] MILLS, P.R.; SREENIVASAPRASAD, S.; BROWN, A.E. Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR. FEMS Microbiology Letters, v.98, p.137-144, 1992.

[18] MORAL, J.; BOUHMIDI, K.; TRAPERO, A. Influence of fruit maturity, cultivar susceptibility, and inoculation method on infection of olive fruit by Colletotrichum acutatum Plant Disease, v.92, n.10, p.1421-1426, 2008. http://apsjournals.apsnet.org/doi/pdfplus/10.1094/pdis-92-10-1421

[19] OPENSHAW, K. A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass and Bioenergy, v.19, n.1, p.1-15, 2000. http://pdn.sciencedirect.com/science?_ob=miamiimageurl&_cid=271477&_user=687369&_pii= s0961953400000192&_ check=y&_origin=browse&_zone=rslt_list_item&_coverdate=2000-07-01&wchp=dglbvlk-zskwz&md5=f993cf141b7616fa70fb0988a361f432/1-s2.0-s0961953400000192-main

[20] PEGG, K.G.; COATES, L.M.; KORSTEN, L.; HARDING, R.M. Foliar, fruit and soil diseases. In: WHILEY, A.W.; SCHAFFER, B.; WOLSTENHOLME, B.N. (Ed.). The avocado: botany, production and uses. Wallingford: CAB International Press, 2002. p.299-338.

[21] PEREIRA, O.L.; DUTRA, D.C.; DIAS, L.A.S. Lasiodiplodia theobromae is the causal agent of a damaging root and collar rot disease on the biofuel plant Jatropha curcas in Brazil. Australasian Plant Disease Notes, v.4, n.1, p.120-123, 2009. http://www.publish.csiro.au/?act=view_file&file_id=dn09049.pdf

[22] PRING, R.J.; NASH, C.; ZAKARIA, M.; BAILEY, J.A. Infection processes and host range of Colletotrichum capsici. Physiological and Molecular Plant Pathology, v.46, n.2, p.137-152, 1995. http://www.dfid.gov.uk/r4d/output/12324/default.aspx

[23] PRUSKY, D. Pathogen quiescence in postharvest disease. Annual Review of Phytopathology, v.34, p.413-434, 1996. http://www.annualreviews.org/doi/pdf/10.1146/annurev.phyto.34.1.413

[24] PRUSKY, D.; KEEN, N.T. Involvement of preformed antifungal compounds in the resistance of subtropical fruits to fungal decay. Plant Disease, v.77, n.1, p.114-117, 1993. http://www.apsnet.org/publications/plantdisease/backissues/documents/1993articles/ plantdisease77n02_114.pdf

[25] RAJ, S.K.; SNEHI, S.K.; KUMAR, S.; KHAN, M.S.; PATHRE, U. First molecular identification of a begomovirus in India that is closely related to Cassava mosaic virus and causes mosaic and stunting of Jatropha curcas L. Australasian Plant Disease Notes, v.3, p.69-72, 2008. http://www.springerlink.com/content/u76424vwh6411050/fulltext.pdf

[26] REUVENI, M.; TUZUNS, S.; COLE, J.S.; SIEGEL, M.R.; KUÉ, J. The effects of plant age position on the susceptibility of tobacco to blue mold caused by Peronospora tabacina Phytopathology, v.76, n.4, p.455-458, 1986. http://www.apsnet.org/publications/phytopathology/backissues/documents/1986articles/ phyto76n04_455.pdf

[27] SÁ, D.A.C.; SANTOS, G.R.; FURTADO, G.Q.; LEMUS, E.A.; NASCIMENTO, I.R. Transporte, patogenicidade e transmissibilidade de fungos associados às sementes de pinhão manso. Revista Brasileira de Sementes, v.33, n.4, p.663-670, 2011. http://www.scielo.br/pdf/rbs/v33n4/08.pdf

[28] SANTOS, G.R.; CASTRO NETO, M.D.; RAMOS, L.N.; CAFÉ-FILHO, A.C.; REIS, A.; MOMENTÉ, V.G., PELÚZIO, J.M.; IGNÁCIO, M. Reaction of melon genotypes to the gummy stem blight and the downy mildew. Horticultura Brasileira, v.27, n.2, p.160-165, 2009. http://www.scielo.br/pdf/hb/v27n2/v27n2a07.pdf

[29] SMITH, B.; BLACK, L.L.; GALLETTA, G.J. Resistance to Colletotrichum fragariae strawberry affected by seedling age and inoculated method. Plant Disease, v.74, n.12, p.1016-1021, 1990. http://www.apsnet.org/publications/plantdisease/backissues/documents /1990articles/plantdisease74n12_1016.pdf

[30] SUTTON, B.C. The genus Glomerella and its anamorph Colletotrichum In: Bailey, J.A.; & Jeger, M.J. (Eds.) Colletotrichum - Biology, Pathology and Control Wallingford: CAB International, 1992. p.1-26.

[31] TANAKA, M.A.S.; PASSOS, F.A.; ITO, M.F. Influência da cultivar e do estádio fenológico do fruto de morangueiro sob o desenvolvimento de lesões causadas por Colletotrichum spp. Summa Phytopathologica, v.20, n.3, p.160-163, 1994.

Brasil

Brasil

Etiology and pathogenicity of two different isolates of Colletotrichum spp. obtained from physic nut seeds

Etiologia e patogenicidade de dois diferentes isolados de Colletotrichum spp. obtidos de sementes de pinhão manso

Abstracts

Publication Dates

History