Activity of antioxidant enzymes and proline accumulation in Erythrina velutina Willd. seeds subjected to abiotic stresses during germination

Atividade de enzimas antioxidantes e acúmulo de prolina em sementes de Erythrina velutina Willd. submetidas a estresses abióticos durante a germinação

Abstracts

The aim of this study was to evaluate the effect of different abiotic stresses on the activity of antioxidant enzymes and on accumulation of proline in Erythrina velutina Willd. seeds during germination. Mulungu seeds were scarified and placed to germinate at constant temperatures of 15, 25, and 35 ºC, moistened with distilled water, and exposed to 12 h of light. Other seeds were exposed to solutions of NaCl (EC of 0, 4, and 8 dS.m-1) and polyethylene glycol (osmotic potentials of 0.0, -0.2, and - 0.6 MPa) and maintained in a germination chamber set at 25 ºC and 12 h photoperiod for seven days. At the end of each period of imbibition, the embryonic axis and cotyledons of the seedlings were collected separately and used to quantify proline content and the activity of antioxidant enzymes. These were detected in both the cotyledons and embryonic axis of the mulungu seeds. Antioxidant activity varied depending upon the type and degree of stress applied. It was concluded that under the aspect of the detoxification process, the mechanism found in mulungu seeds is more efficient when subjected to different temperatures followed by salt stress and water stress.

antioxidative metabolism; water stress; salt stress; heat stress; mulungu

Objetivou-se com este trabalho avaliar o efeito de diferentes estresses abióticos na atividade das enzimas antioxidantes e no acúmulo da prolina em sementes de Erythrina velutina Willd. durante a germinação. Sementes de mulungu foram escarificadas e colocadas para germinarem nas temperaturas constantes de 15, 25 e 35 ºC, umedecidas com água destilada e com 12 h de luz. Outras sementes foram expostas a soluções teste de NaCl (condutividade elétrica 0, 4 e 8 dS.m-1) e soluções de polietilenoglicol (potencias osmóticos 0,0; -0,2 e -0,6 MPa) e mantidas em câmaras de germinação ajustadas a 25 ºC, fotoperíodo de 12 h durante sete dias. Os cotilédones e eixo embrionário de plântulas foram coletados e utilizados para quantificar o teor de prolina e a atividade de enzimas antioxidantes. Estas foram detectadas nos cotilédones e eixo embrionário de sementes de mulungu. A atividade antioxidante foi variável dependendo do tipo e nível de estresse aplicado. Os resultados encontrados permitem concluir que sob o aspecto do processo de desintoxicação, o mecanismo encontrado nas sementes de mulungu é mais eficiente quando submetidas a diferentes temperaturas, seguido pelo estresse salino e estresse hídrico.

metabolismo antioxidativo; estresse hídrico; estresse salino; estresse térmico; mulungu

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Publication Dates

• Publication in this collection
  24 June 2014
• Date of issue
  June 2014

History

• Accepted
  19 Mar 2014
• Received
  22 Jan 2014