LEITAO, Cintia Camurça Fernandes et al. Goat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro. Cienc. Rural [online]. 2013, vol.43, n.1, pp.107-113.
Epub 22-Nov-2012. ISSN 1678-4596.
Research studies the process of oocyte maturation of ovarian follicles of goats
Researchers of Universidade Federal do Ceará, Sobral, in Brazil, showed that goats ovarian follicles express different levels of mRNA for the βA subunit of inhibin, and activin-A promotes the growth of secondary follicles in vitro. The study was published in the journal Ciência Rural, v.43, n.1, January 2013.
The researchers conducted an experiment with secondary follicles cultured for six days. During that period, they evaluated the effects of activin A (100 ng ml-1) and / or follicle stimulating hormone (FSH, 50ng ml-1) on the growth and the expression of mRNA for βA inhibin and FSH receptors (FSH-R). The results show that the expression of inhibin- βA is lower than secondary follicles and primary follicles is greater in large antral follicles compared to small antral follicles. The activin-A and / or FSH promoted the growth of secondary follicles in vitro. FSH increased levels of mRNA for inhibin- βA, while activin-A increased the levels of mRNA for FSH-R.
The study in ovarian physiology of mammalian and of oocyte maturation has great significance due to the low yield of the mammalian ovary that releases few viable oocytes for fertilization. Furthermore, the researcher José Roberto Viana Silva points out that evaluation of the levels of mRNA for important factors for oocyte maturation, such as inhibin-βA, helps to understand the processes of maturation and early embryonic development.
According to José Roberto Silva Viana, the study developed opens numerous possibilities for the field of animal reproduction. "With the research, we have obtained useful information for development of a culture system that allows for the in vitro maturation of oocytes of thousands present in mammalian ovaries. This can be used for future production of embryos from high-value livestock or endangered animals", he reports.