LASKOSKI, Luciane Maria et al. Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment. Cienc. Rural [online]. 2013, vol.43, n.1, pp. 66-72. ISSN 0103-8478. http://dx.doi.org/10.1590/S0103-84782013000100012.
Research investigates physiopathogeny of laminitis in horses
Researchers from Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP - Jaboticabal) and from Universidade de Campinas (UNICAMP), with collaboration of researchers of other universities, demonstrated that intestinal chirurgical proceedings and troubles obstructers in horses can cause laminitis. The study was published in Ciência Rural journal, v.43, n.1, of January 2013.
The researchers used as experiment twenty horses to compose the control group (Cg), instrumented group (without intestinal obstruction), treated group (submitted to intestinal obstruction and treatment) and non-treated group (submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae.
Results indicated an increasing of MMP-2 and MMP-9 activities in laminae tissue after the intestinal chirurgical intervention and, also, after intestinal obstruction. The researcher Luciane Laskoski explains that metalloproteinases are enzymes released by neutrophils and macrophages that serve as indicators of inflammatory processes in tissue. They are associated to the development of laminitis.
The importance of the research is to study laminitis, a disease that affects horses and that does not have its physiopathogeny known. In this sense, Luciane Laskoski mentions that the contribution of the study is to provide information to the understanding of the disease mechanism. "Only after knowing the physiopathology is that you can find effective methods of treatment for laminitis," says the researcher.
The study innovates in its methodology, because it sought to study laminitis by inducing gastrointestinal condition, since the condition often occurs secondary to gastrointestinal disorders. The researchers used as base a survey conducted in 2004, which followed in the same direction for the evaluation of gastrointestinal disorders to evaluate the laminar tissue of the hoof. Aside from these two studies, according to Luciane, most of the studies seek to identify the physiopathogeny of laminitis by inducing the disease, by administration of corn starch.