Resumo em Inglês:

Abstract The ecological and ethnological aspects of the harvesting of the cockle, Leukoma pectorina (Bivalvia: Veneridae), were investigated on Algodoal-Maiandeua Island, on the Amazon coast of Brazil. Ethnobiological data were collected through informal conversations, semi-structured interviews, and observations of the harvesting and processing of bivalves on the island. Following the ethnobiology study, the cockle beds were surveyed to evaluate the density of L. pectorina, body size and the meat yield of the cockles in the months of dry and rainy seasons. In the study area, cockling is a manual and artisanal activity, and L. pectorina is typically harvested by mothers with little formal education. The cocklers make their own tools, cockles are prepared in the family environment, and the majority of the catch is sold to commercial establishments on the island. Cockling is a sporadic activity used to complement the family income, and is more common during the dry season, when tourism increases on Algodoal-Maiandeua Island. The cockles are also larger and population density is higher during this season, and the cocklers themselves recognize this period as providing the most productive harvest. These findings reinforce the value of traditional knowledge for both scientific research and the planning of the management of coastal fishery resources.

Resumo em Inglês:

Abstract In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated. It was observed that Tween 40 and ethanol displayed a stimulatory effect on EPS secretion. The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively. Monosaccharide composition analysis indicated that EPS-T, EPS-E and EPS-C were mainly composed of mannose and glucose. Furthermore, EPS-E exhibited better OH• and DPPH scavenging activities than those of EPS-C and EPS-T, which might be associated with its molecular characterization.

Resumo em Inglês:

Abstract The size at the onset of sexual maturity (SOM) was determined in four hololimnetic populations of Macrobrachium amazonicum using morphometric, physiological and functional criteria. Male prawns from two populations analyzed had hypertrophied chelipeds and large body proportions, showing the presence of four morphotypes, i.e. large-size phenotype (LS). However, the other two populations showed smaller males without morphotypes, i.e. small-size phenotype (SS). The development of sexual weapons in males modulated the mating system and SOM in this species. It was verified that there is a synchrony between physiological and functional maturities in SS males. On the other hand, functional maturity in LS males occurred after physiological and morphological maturities. In females, we verified synchronization between morphological and functional maturities. The results obtained indicated that, in both sexes, physiological maturity anticipates the others criteria. There were no differences between the sequential scheme of sexual maturity for female phenotypes, being: physiological → functional morphological. However, the evaluation of sexual maturity in males must take into account the phenotype of population, since that was modulated by functional maturity. The sequential scheme for LS males is: physiological → morphological → functional, while for SS males it is: physiological = functional → morphological.

Resumo em Inglês:

Abstract The spore morphology and wall ultrastructure of Dryopteris filix-mas, D. patula and D. wallichiana from Argentina were studied using light microscope, scanning and transmission electron microscope. The study was carried out with herbarium material from Argentine institutions. Equatorial diameters, polar diameters and laesura length were measured. The spores are monoletes with a rugate ornamentation. The folds are short to long, inflate, irregular in shape and size, and varying from subglobose to elongate. The perispore surface is rugulate. The exospore of all the species analyzed is two-layered in section. Simple and branched channels are also present. The perispore is composed of two layers, the inner one forms the ornamentation and the outer covers all the outer and inner surfaces. Some abnormalities, such as globose, triangular or twisted spores were observed. The morphology and ultrastructure of the species are very similar. The differences observed are related to the length and thickness of the perispore folds. The characteristics of these spores would not provide relevant information to differentiate species or sections within the genus, but can provide information for phylogenetic studies as well as for alterations in the biological cycles.

Resumo em Inglês:

Abstract This study investigated the occurrence of individuals of stingless bees attracted by the trophic resources existing in four edge areas of forest fragments located in the Baturité Massif, State of Ceará. In this sense, the study evaluated the species richness in the areas; the absolute abundance of species; the similarity of species between edges; and the affinity of bees for the same plant species. The sampling of bees compiled a total of 20 species distributed in 12 genera. Seven species stood out for abundance and two (Trigona spinipes and Trigona fulviventris) showed dominance. The results also showed that the fragments present a similar stingless bee fauna and the affinity of the bee species for the same plant species revealed the formation of five distinct bee groups in foraging preferences involving 15 (75%) out of the 20 species of bees studied.

Resumo em Inglês:

Abstract An updated and annotated checklist of mammals occurring in Brazil is presented. A total of 751 native species, distributed in 249 genera, 51 families and 11 orders were recorded to the country. The Brazilian mammalian fauna shows an elevated rate of endemism (30%; 223 species). Among the species evaluated by IUCN (668 species; 90%), a total of 80 (10.6% of total mammalian fauna) are Threatened, 28 (3.9%) are considered as Near Threatened, two species (0.3%) are presumable Extinct, 96 (12.8%) are considered with Deficient Data for conservation and 462 (61.6%) are considered as Least Concern. Fifteen new species were described since the last national compilation (published in 2017), which associated to new records to the country and synonimizations resulted in an increment of 30 species. Eight non-native species were introduced to the country, including the recently established Asiatic cervids Rusa unicolor (sambar) and Axis axis (chital). Seven native species (five primates and two hystricomorph rodents) have been translocated from their areas of natural occurrence to other areas inside the country.

Resumo em Inglês:

Abstract Iron and zinc bioaccumulation in mycelial biomass of different medicinal basidiomycetes was evaluated in order to produce metal-enriched mycelial biomass as an alternative functional food from non-animal sources and based on biotechnology processes. Pleurotus ostreatus strain U2-9, U2-11, U6-8, and U6-9, Pleurotus eryngii strain U8-11, Schizophyllum commune strain U6-7, and Lentinula edodes strain U6-11 and U6-12 were grown in malt extract agar with or without addition of 50 mg/L iron or 7.5 mg/L zinc. The mycelial biomass was separated and iron and zinc concentrations were determined in a flame atomic absorption spectrophotometer. Basidiomycete strains presented different growth rates with the presence of iron and zinc; there was no dependence between the metal bioaccumulation and the fungal growth. The fungi presented greater capacity to bioaccumulate iron than zinc. P. ostreatus (U2-9) has greater iron bioaccumulation (3197.7 mg/kg) while P. ostreatus (U6-8) greater zinc bioaccumulation (440.4 mg/kg) in mycelial biomass. P. ostreatus (U2-9), P. ostreatus (U2-11), and S. commune (U6-7) had the highest metal translocation rates from the culture medium to mycelial biomass. The mycelial biomass enriched with iron or zinc is an alternative to a new functional food from non-animal sources.

Resumo em Inglês:

Abstract The restinga is a threatened Brazilian ecosystem and a highly heterogeneous environment. This work aimed to evaluate demographic and genetic aspects of Varronia curassavica and whether environmental heterogeneity can influence the studied population parameters. Three annual evaluations were carried out in an area of restinga in Florianópolis-SC, Brazil. Demographic data were analyzed using descriptive statistics, and the spatial distribution pattern was calculated by Ripley’s K-function and correlated with environmental characteristics. To characterize diversity and genetic structure, eight microsatellite markers were used. This work demonstrated that variations in the distribution of individuals and genotypes can be related to specific environments. Dry lowlands were environments favorable to population development, and flooded lowland and mobile dunes were unfavorable. The fixation indices were distinct between environments, evidencing a tendency toward preferential crosses in favor of heterozygotes. We found absence of spatial genetic structure, indicating that genotypes are randomly distributed and that gene flow may be related to such genetic factors as the presence of autoincompatibility mechanisms. This diversity of environments contributed to the aggregate distribution and is relevant for the maintenance of demographic and genetic processes of the species in restingas, and this aspect should be considered for in situ conservation.

Resumo em Inglês:

Abstract In 2012, a severe drought struck the southeastern of Brazil compromising the Paraná River Basin reservoirs. Here, we described how this climatic event promoted a massive mortality of the giant freshwater mussel Anodontites trapesialis in Furnas reservoir and reported the consequences of this phenomenon. In November 2012, three quarters of 100 m2 were sampled in this reservoir, where 812 dead shells of A. trapesialis were analyzed and measured (33 ˫ 133 mm). The species showed an aggregated distribution with high density (X¯: 1.0 - 5.5 ind/m2). Despite the massive mortality detected in field, it was possible to find living specimens in a small channel in the studied area, allowing the species to survive the water level fluctuations. Large adult individuals (100 ˫ 124 mm) were more affected by drought than juveniles, accounting for about 90% of the dead mussels analyzed. Two years after the massive mortality event, water level was not reestablished and a terrestrial succession (with elevations in the concentration of organic matter and calcium in sediment) was observed in the studied area. We verified that the damming associated with extreme climatic events affect negatively the populations of A. trapesialis and should be faced as a conservationist problem.

Resumo em Inglês:

Abstract Organic selenium, tellurium and sulfur compounds have been studied due to their pharmacological properties. For instance, the β-aryl-chalcogenium azide compounds have demonstrated antitumoral action in vitro. However, yet no pharmacological actions of this class of compounds were determined in vivo. Caenorhabditis elegans is a nematode that presents innumerable advantages in relation to mammalian models, such as having a small and transparent body, which allows the visualization of its internal anatomy, besides short life and low cost. Based on that, the aim of this work was to investigate the pharmacological and toxicological properties of β-aryl-chalcogenium azide compounds in C. elegans. As well, to evaluate the capacity of organochalcogenium compounds to repair oxidative damage induced by hydrogen peroxide and the possible mechanism of action of these compounds using CF1553 transgenic strain with superoxide dismutase (SOD-3) tagged with GFP. Our results showed that β-aryl-chalcogenium azide have low toxicity in wild-type worms and the pre-treatment protected against the damage induced by hydrogen peroxide at higher tested concentration. Associated with this, we observed that this protection is due in part to the increased expression of the antioxidant enzyme SOD-3. In conclusion, β-aryl-chalcogenium azide compounds caused low toxicity and induced stress-resistance by modulating SOD-3 expression in C. elegans.

Resumo em Inglês:

Abstract The species of the genus Arachis (Leguminosae) are ordered into nine sections. The assignment of genome types in this genus has been based on cross-compatibility analysis and molecular cytogenetic studies. The latter has also allowed karyotypically establishing well-defined genomes and reassigning the genome of several species. However, most of these studies have been focused mainly on the sections Arachis and Rhizomatosae. To increase the knowledge about the chromosome diversity of the whole genus, here we performed a detailed karyotype characterization of representative species of most of the sections and genomes of Arachis. This characterization included chromosome morphology, CMA/DAPI chromosome banding, and chromosome marker localization (rDNAloci and one satDNA sequence) by fluorescent in situ hybridization (FISH). Based on the data obtained and other previously published data, we established the karyotype similarities by cluster analysis and defined eleven karyotype groups. The grouping was partly coincident with the traditional genome assignment, except for some groups and some individual species. Karyotype similarities among some genomes were also found. The main characteristics of each karyotype group of Arachis were summarized. Together, our results provide information that may be beneficial for future cytogenetic and evolutionary studies, and also contribute to the identification of interspecific hybrids.

Resumo em Inglês:

Abstract Metacercariae of Diplostomidae are widely distributed in America and may cause diplostomiasis, an ocular disease in fishes. The aim of this study is to report the occurrence of metacercariae of Austrodiplostomum compactum in Plagioscion squamosissimus (non-native fish species) from Nova Avanhandava Reservoir, Tietê River, Brazil and an explanation for the high infection rates with this parasite in the Paraná River Basin is proposed. Eyes of 70 hosts were examined, the metacercariae were preserved and identified. The prevalence (P), mean intensity of infection (MII) ± standard deviation, mean abundance (MA) ± standard deviation, were calculated and a bibliographic review was performed. There was no difference in parasitism between male and female hosts. The values of P = 80%, MII = 21.55 ± 3.25 and MA = 17.24 ± 2.91 were high, as in most studies in areas where P. squamosissimus were introduced, while these values were low in areas of natural occurrence. This may be explained by the genetic susceptibility of the host to the parasite. The entire population of P. squamosissimus from the Upper Paraná has been founded by a few specimens, resulting in very low genetic variability. Consequently, the population may be highly susceptible to A. compactum.

Resumo em Inglês:

Abstract An extensive field survey was conducted to evaluate the spreading of the invasive estuarine bivalve Mytilopsis leucophaeata into the metropolitan region of Rio de Janeiro (Brazil), in which the single invaded site so far recorded is Rodrigo de Freitas Lagoon. A novel record is made to the Marapendi coastal lagoon, where M. leucophaeata mainly colonizes mangrove roots. A reef composed of druses above the unconsolidated substratum is recorded for the first time for this species. The fauna associated to agglomerates of M. leucophaeata was composed of eight taxa: polychaetes Alitta succinea and Polydora sp., amphipods Melita mangrovi and Elasmopus sp., barnacles Amphibalanus eburneus and A. improvisus, the crab Eurypanopeus cf. dissimilis and the gastropod Heleobia spp. Based on COI sequences, the haplotype diversity of M. leucophaeata invasion in Marapendi Lagoon is high, like that previously recorded in Rodrigo de Freitas Lagoon. The expansion of M. leucophaeata demands a continuous investigation, including the knowledge of its impacts on the biota.

Resumo em Inglês:

Abstract The aim of this study is to investigate the richness and composition of the bryophytes of two Municipal Natural Parks in Amapá, eastern Amazon. Bryophytes were collected in the Arivaldo Gomes Barreto Municipal Natural Park (AGBMNP), Macapá municipality, and Cancão Municipal Natural Park (CMNP), Serra do Navio municipality, in October 2010 and October 2012, respectively. Ten plots of 10 x 10 m were established in each park for sampling, and, in addition, random collections were made, in order to maximize the knowledge on the local bryophyte flora. In total, 53 species were identified in AGBMNP, and 110 species in CMNP. The results shown a lower richness in AGBMNP than in CMNP, which may be related to the own size of each park, the level of conservation, and its localization. In terms of abundance, the neotropical pattern and the species locally rare were most representative in both parks, however, the floristic composition diverged. The AGBMNP is composed mostly by generalist species, while the CMNP by both generalists and shade specialists. The present study recorded 54% of the known bryophyte flora of Amapá, 63 new records for the state and, in addition, three new records for the North region of Brazil.

Resumo em Inglês:

Abstract EHV-1 infection is responsible for huge economic losses in equines due to abortion and neonatal mortality. In this study, we describe 4 cases of abortion and neonatal deaths from pregnant mares and a she-donkey from different localities in Egypt during the period from May 2015 to October 2017. Attempts were made to isolate and identify EHV-1, in addition to compare the different pathological lesions in various tissues of the necropsied cases. EHV-1 was successfully isolated from two aborted fetuses and one dead neonatal foal from mares, beside one aborted fetus from a she-donkey. The positive cases showed cytopathic effect on embryonated chicken eggs scattered on chorioallantoic membrane. Moreover, PCR was applied for the pock lesions and revealed positive results for EHV-1. Interstitial pneumonia, bronchopneumonia and necrosis of hepatic, myocardial, microcotyledonary tissues besides disseminated thrombi were the main encountered lesions. Intranuclear inclusion bodies were demonstrated in brain, liver, placenta and pulmonary tissues. Here, we describe EHV-1 induced brain lesions represented by degenerated neurons, vascular endotheliosis with intranuclear inclusion bodies in the aborted she-donkey fetus. Lesions were more sever in the aborted fetuses from mares than the one from the she-donkey. EHV-1 antigen was detected by immunohistochemistry staining.

Resumo em Inglês:

Abstract We evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different configurations: static flat-bed, plugged-flow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defined as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 oC, in static flat-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-flow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterification and transesterification reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst.

Resumo em Inglês:

Abstract To study the ontogeny of the extrafloral nectaries present in the inflorescences of Vigna luteola (Jacq.) Benth (Leguminosae, Phaseolinae), the location, morphology, anatomy of the earliest stages, histology of the definitive structures and ultrastructure of the secretory stage were analyzed. The extrafloral nectaries at different developmental stages were examined with light microscopy and scanning electron microscopy. The secretory stage was also examined with transmission electron microscopy. The racemose inflorescence of V. luteola has six nodes. At each node, a short globose secondary axis bears two flowers and one to three extrafloral nectaries. Each extrafloral nectary originates from the abscission of a flower bud and is formed by two differentiated zones: a ring of epidermal cells surrounding a group of longitudinally enlarged papillose central cells, both with underlying secretory parenchyma. The primary secretory tissue consists of the central cells, while the ring contributes to secretion to a lesser degree. Secretion is granulocrine, by means of exocytotic vesicles and plasmalemma invaginations. Four developmental stages succeed; the third one being the secretory. The extrafloral nectaries activity period starts when the flowers of the same secondary axis open and ceases before fruit development.

Resumo em Inglês:

Abstract We analyzed reproductive biology of Erythrolamprus jaegeri coralliventris, a snake from the Brazilian Pampa. Females presented larger snout-vent length than males, while no significant differences were found in tail length/snout-vent length ratios between sexes. Females attain sexual maturity in larger sizes than males. The reproductive cycle of females presented a seasonal pattern, with advanced vitellogenesis occurring from middle winter to middle spring and oviductal eggs occurring from middle winter to middle summer. The real fecundity ranged from two to eigth eggs and the expected fecundity varied from one to 12 secondary follicles. No significant correlation was found between females body size and the following parameters: real fecundity, length of the largest egg and potential fecundity. Therefore, E. j. coralliventris presents a seasonal reproductive pattern, which seems to follow the rainfall profile observed for the studied region. This may represent a strategy of energy gain associated to the reproductive cycle, considering that the food resources most explored by this species are anurans that present higher activity during rainy periods.

Resumo em Inglês:

Abstract In Patagonia, knowledge about the interaction among tephritids and the native flora is very scarce. In this study we identified for the first time two tephritid species (Cecidochares sp. and Neosphaeniscus m-nigrum) associated with the capitula of Chuquiraga avellanedae. This is the first record of a host plant for the genus Neosphaeniscus. Cecidochares sp. was more abundant and had a shorter development time than N. m-nigrum. Also, two families of parasitoid wasps (Pteromalidae and Eurytomidae) were registered. Further studies are needed to understand the impact of these tephritids on C. avellanedae fitness and their potential to control its populations.

Resumo em Inglês:

Abstract The aims of this work were to improve cell tolerance towards high concentrations of furfural and 5-hydroxymethylfurfural (HMF) of an osmotolerant strain of Wickerhamomyces anomalus by means of evolutionary engineering, and to determine its ethanol production under stress conditions. Cells were grown in the presence of furfural, HMF, either isolated or in combination, and under high osmotic pressure conditions. The most toxic condition for the parental strain was the combination of both furans, under which it was unable to grow and to produce ethanol. However, the tolerant adapted strain achieved a yield of ethanol of 0.43 g g-1glucose in the presence of furfural and HMF, showing an alcohol dehydrogenase activity of 0.68 mU mg protein-1. For this strain, osmotic pressure, did not affect its growth rate. These results suggest that W. anomalus WA-HF5.5strain shows potential to be used in second-generation ethanol production systems.

Resumo em Inglês:

Abstract Erythrodiplax nataliae sp. nov. (5 males), collected in Vereda wetlands (a unique Neotropical savanna environment) in Mato Grosso, Brazil is described and illustrated. The new species fits in Borror’s Juliana Group, and can be distinguished from other species by the combination of the following traits: blue pruinosity on thorax (more dense dorsally); sides of the pterothorax yellowish, darkening dorsally; face ivory, dorsally black with a metallic blue reflection; wings hyaline with a small basal brown spot; vesica spermalis with long lateral lobes, enclosing the median process and median process elongated with a pair of conspicuous rectangular and elongated lateral lobes, with a middle dorso-ventral furrow.

Resumo em Inglês:

Abstract Knowing about the spatio-temporal patterns in the structure of ichthyoplankton assemblages allows inferring about the spawning behaviour of adult fishes, understanding the recruitment dynamics, and predicting the potential effects of mid- and long-term changes. Here, we studied the ichthyoplankton assemblages from the San José Gulf (Northern Patagonia, Argentina) and investigated their changes in space and time. To do that, we took monthly samples during two consecutive years, in spring and summer. A total of 2088 larvae were caught; they comprised 36 taxa, from which 14 were identified to species, two to genus, one to family and one to order. There were large differences in the structure of the assemblages between years, coincidently with marked changes in the surface water temperature. The structure of the ichthyoplankton assemblages also showed significant differences between the spring and summer: Helcogrammoides cunninghami, Dules auriga and larvae belonging to the family Engraulidae contributed most to these differences. The species diversity was higher in the colder year than in the warmer one. We discuss the potential role of environmental and oceanographic features on the interannual variability in the early stages of coastal fishes within a small gulf.

Resumo em Inglês:

Abstract English is the lingua franca for scientific communication, but some journals, especially in developing countries, still publish non-English studies. A shift towards publishing in English may promote internationalization and more visibility of scientific journals. Here we compared quality indexes between Brazilian journals that have always published in English and journals that have published in languages other than English. We also investigated whether a temporal shift towards publishing in English led to elevated quality measures. Our analyses covered 16 Brazilian biodiversity journals and accounted for 12640 papers published since 2007. The mean impact factor was on average 55% higher in journals that have published consistently in English, compared to the so-called multilanguage journals. The proportion of publications in English increased to nearly three times the original value in multilanguage journals between 2007 and 2016, and the impact factor tripled during this period. At the same time, the Qualis-Capes classifications (B1-B2-B3) tended to fall. Publishing in English can be a first step to increased visibility, and this is particularly important for biodiversity journals, since Brazilian ecosystems are considered of interest to the international scientific community and nature conservation.

Resumo em Inglês:

Abstract This study aimed to investigate antigenotoxicity and antioxidant potential of extract, fractions and vitexin from C. antisyphiliticus. Methanolic extract was fractionated through solvents of increasing polarity. The composition of extracts and fractions were evaluated through phytochemical screening. Micronucleus test was performed in mice to evaluate the antigenotoxicity. Antioxidant activity was measured using the assay 1,1-diphenyl-2-picrylhydrazyl (DPPH), iron ion chelating, thiobarbituric acid assay and nitric oxide scavenging. Treatment with extract, fractions and vitexin did not produce an increase in Micronucleus mean values. However, Micronucleus (MN) mean values decreased in relation to control. methanolic extract presented antioxidant potential for DPPH (81%), iron ion chelating (77.8%), Thiobarbituric Acid (TBARS) (32.49%) and Nitric Oxide (NO) (80.97%). Ethyl acetate fraction showed the highest antioxidant activity (65.46%). The vitexin showed a Inhibitory Concentration (IC50) of DPPH value smaller in relation to control. Vitexin flavonoid was detected by High Performance Liquid Chromatography (HPLC), infrared spectrometry and nuclear magnetic resonance. It can be inferred that methanolic extract, fraction ethyl acetate and vitexin isolated from C. antisyphiliticus is endowed with antigenotoxic and antioxidant potential.

Resumo em Inglês:

Abstract The present study describes Trypanosoma spp. infection and blood parameters in Pterygoplichthys pardalis from the Tapajós River basin in eastern Amazon (Brazil). Of 32 fish examined, 40.6% were infected by Trypanosoma spp., while a total of 112 trypomastigotes were found. Two Trypanosoma morphotypes were characterized and compared with species described in literature infecting other Loricariidae, and a similarity of 94% was found with one species described for another host. The plasma glucose and aspartate aminotransferase levels, hematocrit, number of total erythrocytes, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) in the infected and uninfected fish were similar, but hemoglobin was lower in fish infected with Trypanosoma spp. Hemoglobin levels declined with the abundance of the hemoparasites, but the condition factor was similar among fish infected and uninfected by Trypanosoma spp. This is the first study on the hemoparasitism by Trypanosoma spp. and blood parameters in P. pardalis.

Resumo em Inglês:

Abstract This study reports the occurrence of metacercariae of Austrodiplostomum compactum in Pimelodus platicirris from a Neotropical reservoir in the Grande River, SP, Brazil. A total of 164 fish were collected, of which 12.80% were infected with metacercariae in the eyes. The mean intensity of infection and mean abundance were 1.52±0.14 (1‒3) and 0.23±0.05 (0‒3), respectively. The presence of this parasite with a high intensity of infection can cause exophthalmos, retinal displacement, opacity of the lens, blindness or even death. This is the first record of ocular metacercariae for P. platicirris, thus increasing the number of hosts for A. compactum.

Resumo em Inglês:

Abstract Sea anemones are considered as polyphagous opportunistic predators and it has been suggested that their diet reflects the structure of the community they inhabit. The feeding ecology of intertidal species is an interesting topic to study due to their wide variety of strategies to obtain food. In this sense, we studied the diet of Bunodosoma zamponii, the most abundant actiniarian in the rocky intertidal of Punta Cantera (Mar del Plata, Argentina). The objectives were to describe it and compare its composition seasonally and between diurnal and nocturnal high tides. We examined the gastric cavity content of 154 specimens collected seasonally at both diurnal and nocturnal high tides, and 39 different prey items were identified, some of which are recorded here for the first time for this species. No variations on diet composition were found between seasons or between diurnal and nocturnal high tides, suggesting that the food available does not vary either. Bunodosoma zamponii ingests mostly items with low biomass, which contribute to the total biomass ingested in direct proportion to their frequency in the diet. The bivalve Brachidontes rodriguezii was the main prey for the anemone, followed to a lesser extent by certain amphipods, other mollusks and algae.

Resumo em Inglês:

Abstract Staphylococcus aureus (S. aureus) is a highly versatile Gram-positive bacterium that is carried asymptomatically by up to 30% of healthy people, while being a major cause of healthcare-associated infections, making it a worldwide problem in clinical medicine. The adaptive evolution of S. aureus strains is demonstrated by its remarkable capacity to promptly develop high resistance to multiple antibiotics, thus limiting treatment choice. Nowadays, there is a continuous demand for an alternative to the use of antibiotics for S. aureus infections and a strategy to control the spread or to kill phylogenetically related strains. In this scenario, bacteriocins fit as with a promising and interesting alternative. These molecules are produced by a range of bacteria, defined as ribosomally synthesized peptides with bacteriostatic or bactericidal activity against a wide range of pathogens. This work reviews ascertained the main antibiotic-resistance mechanisms of S. aureus strains and the current, informative content concerning the applicability of the use of bacteriocins overlapping the use of conventional antibiotics in the context of S. aureus infections. Besides, we highlight the possible application of these biomolecules on an industrial scale in future work.

Resumo em Inglês:

Abstract Although the effects of nickel chloride (NiCl2) on the immune system have long been recognized, little is known about the effects of nickel (II) on the cell cycle and related signaling events in immune organs, such as cecal tonsil, a key immune organ of chicken. In the present study, we investigated the effect of NiCl2 on the cell cycle of cecal tonsil. The cell cycle was detected by the methods of flow cytometry (FCM), quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). The results showed that dietary NiCl2 in excess of 300 mg/kg caused the G2/M cell cycle arrest and the reduction of cell proportion at S phase of the cecal tonsil. The G2/M cell cycle arrest was accompanied by the up-regulation of p53, p21 protein expression and mRNA expression, and down-regulation of cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and mRNA expression. The data suggested that the cells’ (mainly the T lymphocytes) proliferation in the cecal tonsil was inhibited by the high dietary NiCl2.

Resumo em Inglês:

Abstract During a parasitological survey of the olfactory sacs of 21 species of Rajiformes (Chondrichthyes) from the Southwestern Atlantic Ocean, copepods referable to Dendrapta Kabata (1964) (Siphonostomatoida: Lernaeopodidae) were found parasitizing the cuphead skate Bathyraja scaphiops (Rajiformes: Arhynchobatidae). Morphological analyses using both light and electron microscopy revealed that they belong to a new species. It can be easily distinguished from its congeners by the ratio between lengths of posterior process and trunk (1:0.8), the large to width ratio of trunk (1:0.7) and the armature of the antennule (1, 1, 5 + 1 aesthete). Dendrapta cameroni longiclavata is raised to full specific status, as Dendrapta longiclavata n. comb. Kabata & Gusev, 1966.

Resumo em Inglês:

Abstract Teredinidae (shipworms) is a family of marine wood-boring bivalves that has an important role in the degradation of wood through its symbiotic relationship with cellulolytic bacteria. To evaluate the rate of degradation of wood by teredinids in two sites with different oceanographic conditions in Rio de Janeiro State, Brazil, artificial structures composed of pine wood sheets were immersed in the ocean for three months at Arraial do Cabo in an area under the influence of upwelling, and at Ilha Grande Bay under tropical and oligotrophic influences. After the immersion period, teredinids were removed from the collectors, identified, and counted. Wood consumption by the teredinids was quantified by comparing the dry weights of the collectors before and after immersion. Associated bacteria were isolated and their cellulolytic activities evaluated at different temperatures and salinities. Two Teredinidae species were recorded: Bankia gouldi and Lyrodus floridanus. The highest wood degradation rate and enzymatic activities of the isolated bacterial strains were recorded at Arraial do Cabo, suggesting that upwelling influenced the activities of those species.

Resumo em Inglês:

Abstract The structural complexity of galls depends on species-specific interaction driven by the galling taxa. However, the host plant and environment stressors can impose limits on gall developmental patterns and impact the establishment of gall morphology. Herein, we employed qualitative and quantitative approaches in order to elucidate how cell divisions, elongation patterns, and tissue organization are determinant for the development of intralaminar gall morphology induced by Pseudophacopteron longicaudatum Malenovský, Burckhardt, Queiroz, Isaias & Oliveira (Hemiptera: Psylloidea: Phacopteronidae) on leaves of Aspidosperma tomentosum Mart. (Apocynaceae). In addition, we aimed to determine which anatomical process can discriminate the stages of gall development, plus, examine the histochemical and cytological profiles of the galls. The differentiated structures, mainly abaxial epidermis and spongy parenchyma, are associated with gall closure, with hyperplastic events concentrated in the young phase of the galls. Thus, epidermis and spongy parenchyma hypertrophy and are responsible for the determination of the nymphal chamber formation and gall shape. The mature galls do not differentiate into a typical nutritive cells and do not develop a histochemical gradient in their tissues. The cytological features of galls such as plastoglobules and multivesicular bodies are related to ROS scavenging mechanisms due the high oxidative stress.

Resumo em Inglês:

Abstract This study investigated the impact of supplementing normal and high-fat diets with graded levels of lycopene on the growth performance, cholesterol level of the muscle, and antioxidant markers in Japanese quail. A total of 192, 14 day-old unsexed Japanese quail were part of a 2 x 4 factorial arrangement consisting of a control group; birds that were fed a normal fat diet (NFD), another control group; birds that were fed a high-fat diet (HFD) with four levels of lycopene for NFD and HFD (0, 100, 200 and 300mg lycopene/kg diet). Lycopene level of 300mg/kg gave the greatest body weight, body weight gain, and relative growth rate when added to the NFD, but this level showed non-significant improvement in growth performance when supplemented to an HFD. Superoxide dismutase in the muscle and liver was noted to be high in NFD+ 300mgL, HFD+ 200mgL, and HFD+ 300mgL groups, while malondialdehyde level in the muscle and liver and cholesterol level in the muscle was found to be low in the same groups. Lycopene slightly improved growth performance, but significantly improved the antioxidant status and lowered cholesterol concentration in the muscle. A diet supplemented with 300 mg lycopene/kg could be recommended for Japanese quail.

Resumo em Inglês:

Abstract Tanniferous plants have been used for ruminants verminosis control and represent a possibility to minimize the pharmacological resistance against conventional antiparasitics. This study aimed to evaluate the antihelminthic activity of the hydroalcoholic extract of stem bark of guava tree (PgHA). It was performed the hatchability and larval migration inhibition assays to evaluate PgHA at the following concentrations 0.62, 1.25, 2.5 and 5.0 mg mL-1 and the control treatments. The total polyphenol, flavonoid and tannin contents were determined by phytochemical analysis, high performance liquid chromatography coupled to mass spectrometry. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl, ferric reducing antioxidant power and thiobarbituric acid reactive substances tests. It was also determinated total protein, intracellular H2O2 and antioxidant activity of enzimes: glutathione S-transferase and superoxide dismutase. PgHA was able to inhibit both hatchability and larval migration, but only hatchability inhibition presented dose-dependent pattern. The antioxidant activity was demonstrated by linear regression with IC50 corresponding to 534.02 μg mL-1. The antiparasitic mechanism occurred through pro-oxidative activity by the increase of total proteins, intracellular H2O2 and the lipid peroxidation products, as well as the increase of the enzymes above related. Thus, the PgHA showed antiparasitic activity in vitro.

Resumo em Inglês:

Abstract In order to characterize Patagonian (Argentina) ecoregions using non-marine ostracods, their associations in 69 environments were assessed. Twenty eight taxa were recorded, including 12 endemic of the Neotropical region. Our results indicate that Patagonian ostracods are mainly influenced by electrical conductivity (EC), altitude, pH, and temperature; and shows a correlation with Argentinian ecoregions. Assemblage I is composed of sites located at high altitude in the Andean Patagonian forest ecoregion. Host waters have low temperature, EC and pH, and support as representative species Cypris pubera, Eucypris virens, Bradleystrandesia fuscata, Tonacypris lutaria and Amphicypris nobilis. Assemblage II, related to mid-altitude environments in the Patagonian Steppe ecoregion, thrived in waters with moderate to high EC, and alkaline pH values. Dominant species includes Limnocythere rionegroensis, L. patagonica, E. virgata, Riocypris whatleyi, Riocypris sarsi, Newnhamia patagonica, Kapcypridopsis megapodus, Ilyocypris ramirezi and Penthesinelula incae. Assemblage III inhabited environments within Monte and Espinal ecoregions, situated in the eastern part of the study area at low altitude, EC moderate and temperate waters, supporting Heterocypris hyalinus, Amphicypris argentinensis, Sarscypridopsis aculeata, Cypridopsis vidua, Herpetocypris intermedia and Chlamidotheca incisa. Our results indicates that Argentinian Patagonia hosts a diverse ostracod fauna and highlights their capacity as proxies in ecological and palaeoenvironmental studies.

Resumo em Inglês:

Abstract This study aimed to describe the occurrence, morphology and prevalence of Spirocamallanus krameri, a parasite of Hoplerythrinus unitaeniatus collected in state of Pará, Brazil. The morphological and morphometric characteristics are consistent with the species S. krameri, by having a hexagonal mouth opening, 16 cephalic papillae and two lateral papillae on the amphids. The buccal capsule is internally different in both sexes, with (11–13) spiral ridges in males and (13–17) spiral ridges in females. The basal ring is well developed, and three teeth are present in males and absent in females. Ten sessile caudal papillae and a pair of phasmidial pores occur in males. The spicules are small, subequal, 81–101 µm in length and the tail is tapered. The vulva in females is postequatorial, the tail is conical with lateral phasmidial pores. Among the species of Spirocamallanus that have short spicules, the general morphology of S. krameri most resembles that of S. inopinatus, but differs from that species, which have two cephalic teeth visible (absent in S. krameri) and specimens of S. inopinatus do not have sexual dimorphism in the buccal capsule of present in S. krameri.

Resumo em Inglês:

Abstract The production model used in Brazilian pig farms promotes a concentration of animals in small territorial extensions, causing difficulty in disposing of the manure generated, since the economically viable alternative is to use this as an agricultural fertilizer. The objective was to evaluate the effect of swine manure doses on the biological and behavioral parameters in Folsomia candida by ecotoxicological tests. An Ultisol, Oxisol and Tropical Artificial Soil (TAS) were contaminated with 0, 25, 50, 75 and 100 m3 ha-1 of swine manure, to which springtails were exposed for evaluation of mortality, and the doses 0, 10, 15, 20 and 25 m3 ha-1 of swine manure to evaluated reproduction and avoidance. Lethality was observed in all doses as well as all soils, indicating toxicity of the manure. In the reproduction tests dose since 10 m3 ha-1 caused a reduction of juveniles in all soils. Avoidance behavior was observed in all doses of the Oxisol treatments and at 20 and 25 m3 ha-1 in Ultisol treatments. However, in TAS occurred not avoidance response of F. candida. We conclude that the swine manure was toxic to F. candida and the toxicity is dependent on the soil characteristics and the manure concentrations applied.

Resumo em Inglês:

Abstract The reproduction is one of the main events in the life of an organism, and anurans stand out among vertebrates because of the diversity of their reproductive strategies. We studied the reproduction of two syntopic species, Physalaemus aff. albonotatus and P. santafecinus, and comparatively described their reproductive activity pattern, advertisement calls, calling sites, daily calling activity, amplexus behavior, foam nests, and microhabitats in foam nests. In regards to the reproductive activity pattern, both species were defined as prolonged breeders. However, P. santafecinus exhibited a behavior like explosive breeders: it had a faster reproductive response against rains than P. aff. albonotatus. The calling activity was restricted exclusively to night hours in P. santafecinus, whereas P. aff. albonotatus called during both night and day. The advertisement calls of both species showed a rich harmonic structure, and were characterized by a bimodal harmonic dominance. The species differed significantly in microhabitat calling sites, foam nests, and microhabitats in foam nests. Namely, P. santafecinus frequently called and constructed its nests in sites more exposed than those of P. aff. albonotatus. The general differences in reproductive behaviors observed between the species principally agree with their different reproductive activity patterns.

Resumo em Inglês:

Abstract Leishmaniasis is a neglected disease caused by Leishmania. Chemotherapy remains the mainstay for leishmaniasis control; however, available drugs fail to provide a parasitological cure, and are associated with high toxicity. Natural products are promising leads for the development of novel chemotherapeutics against leishmaniasis. This work investigated the leishmanicidal properties of ethanolic extract of Croton blanchetianus (EECb) on Leishmania infantum and Leishmania amazonensis, and found that EECb, rich in terpenic compounds, was active against promastigote and amastigote forms of both Leishmania species. Leishmania infantum promastigotes and amastigotes presented IC50 values of 208.6 and 8.8 μg/mL, respectively, whereas Leishmania amazonensis promastigotes and amastigotes presented IC50 values of 73.6 and 3.1 μg/mL, respectively. Promastigotes exposed to EECb (100 µg/mL) had their body cellular volume reduced and altered to a round shape, and the flagellum was duplicated, suggesting that EECb may interfere with the process of cytokinesis, which could be the cause of the decline in the parasite multiplication rate. Regarding possible EECb targets, a marked depolarization of the mitochondrial membrane potential was observed. No cytotoxic effects of EECb were observed in murine macrophages at concentrations below 60 µg/mL, and the CC50 obtained was 83.8 µg/mL. Thus, the present results indicated that EECb had effective and selective effects against Leishmania infantum and Leishmania amazonensis, and that these effects appeared to be mediated by mitochondrial dysfunction.

Resumo em Inglês:

Abstract In this study, the Gasteruptiidae species found at the Estação Ecológica de Jataí (EEJ), in Luiz Antonio, São Paulo, Brazil, were documented, based on a survey carried out with Malaise, Moericke and light traps that lasted for three years, between January 2007 and December 2009. During the samplings at EEJ, 13 female specimens of Gasteruption Latreille, 1796 were captured: six of G. bispinosum Kieffer, 1904, six of G. brasiliense (Blanchard, 1840) and a single specimen of G. helenae Macedo, 2011 that is the first record for São Paulo state.

Resumo em Inglês:

Abstract Elevation creates a variety of physical conditions in a relatively short distance, which makes mountains suitable for studying the effects of climate change on biodiversity. We investigated the importance of climate and vegetation for the distribution of butterflies from 800 to 1400 m elevation. We sampled butterflies, and woody and rosette plants and measured air temperature and humidity, wind speed and gust, and solar radiation. We partitioned diversity to assess the processes underlying community shifts across altitudes – species loss versus replacement. We assessed the strength of the association among butterfly, vegetation, and climate. Butterfly richness and abundance decreased with altitude, and species composition changed along the elevation. Changes in butterfly composition with altitude were mainly through species replacement and by abundance increases in some species being compensated by decreases in others. Since the floristic diversity decreased with altitude due to soil conditions, and butterflies are closely related to their host plants, this could explain species replacement with altitude. Overall, we found a stronger association of butterfly community with vegetation than climate, but plant community and climate were also strongly associated between them. Butterfly richness was more strongly associated with plant richness than with temperature, while the reverse was true for butterfly abundance, which was more strongly associated with temperature than with plant richness. We must consider the complementary roles of resource and conditions in species distribution.

Resumo em Inglês:

Abstract This study aimed to examine spatiotemporal variations in chironomid assemblages and to detect how environmental variables affect their structure. We sampled seven streams at low and high altitudes in Northwest Argentina under contrasting climate conditions (Puna and Chaco Serrano) during high- and low-water periods. The environmental variables that affected Chironomidae community structure were water temperature, conductivity, hardness, current velocity and type of substrate. Fine substrates, gravel and low water temperature favoured cold stenothermal fauna, composed of Orthocladiinae, Diamesinae and Podonominae specimens in the high-altitude streams, whereas warm waters with low conductivity and higher velocity favoured increased species diversity in lowland streams, where there was greater abundance of Chironominae (which corresponds to warm eurythermal fauna). The studied environments belong to a transition zone that should be preserved where cold stenothermic and warm eurythermal Chironomidae overlap.

Resumo em Inglês:

Abstract In this study we analyzed data on fishing landings of Genidens barbus in the state of São Paulo, Brazil, from 2000 to 2014. An estimation of the total production was obtained through the analysis of 781,856 landings, among which 87% were categorized as artisanal and 13% as industrial. The abundance index showed some stability in the period. However, due to the high number of production units, the fishing effort need to be maintained, given that there is a risk that increased production might affect the abundance of G. barbus. Thus, as alternatives to maintaining marine catfish exploitation in southeastern Brazil under control, the following management actions can be suggested: i) prohibition of fishing activity by the industrial sector; ii) strengthening of inspection of the fleet that is not allowed to participate in the marine catfish fisheries, with emphasis on purse seiners; and iii) maintenance of a closed season for G. barbus, performing an adaptive management of fishing prohibition according to the reproductive biology of the species and with the support of artisanal fishers. These measures have the aim of promoting sustainable exploitation of G. barbus, especially through small-scale fisheries in southeastern Brazil.

Resumo em Inglês:

Abstract Small-scale fisheries is an important factor in the generation of income, jobs and food security. Understanding the characteristics of small-scale fisheries, and the volume of resources and food that they generate can help society recognize their value, lead to the establishment of public policies to improve working conditions and adapt the management of exploited resources for long-term conservation. The objective of this study was to estimate income, production capacity and importance of small-scale fisheries for fisher families of the municipality of Caraguatatuba, São Paulo, Brazil. Fishers were interviewed from May 2012 to June 2013 and categorized according to vessel size and the number of people on the boat and helpers. Monthly income ranged from US$ 566.78 to US$ 1,466.87. The importance of fishing to family income ranged from 50.60% (employee) to 78.25% (entrepreneur). Fishers usually dedicate themselves to this activity for 8.47 to 13.22 hours daily, with daily fish production volume ranging from 42.72 to 122.14 kg/day. Small-scale fisheries involve, either directly or indirectly, about 1,170 people in the municipality and provide a network of social protection due to their local and regional importance.

Resumo em Inglês:

Abstract To compare the effects of two-(2D, microplate) and three-dimensional (3D, alginate) culture systems on the in vitro growth of small antral follicles in cattle, individual follicles were separately cultured in the two culture systems for 8 days. Half of the culture medium was replaced by fresh medium every 2 days; the former medium was used to assess the amount of follicular hormone secretion using ELISA. Individual follicle morphology, diameter, and survival rate were recorded every alternate day. The results showed that in 4 days, there was no significant difference between the two systems, except that the growth rate of follicles in 2D system was relatively faster. After 4 days, estradiol concentration in 3D system was higher than that in 2D system. However, progesterone concentration was lower than that in the 2D system. The survival rate and oocyte quality of follicles in 2D system were significantly lower than those in 3D system on day 8. The follicle diameter slightly increased (30-60 μm) in the entire process. Taken together, for in vitro culture of follicles within 4 days, the 2D culture system is more suitable. However, when the culture duration is >4 days, the 3D culture system is more suitable.

Resumo em Inglês:

Abstract The sewage snail Physa acuta, native to North America, is an effective invasive species around the world. In Chile, it was first reported in 2014 in the north central area of the country. So far, the species has not been recorded in southern Chile. Sampling performed in 2015 in three localities from Llanquihue Lake, Chilean Patagonia, only provided native freshwater snails. However, new collections performed in February 2018 in the same three sites were successful for physid specimens suggesting a biological invasion entailing a large southward range expansion of these snails. Here we performed morphological, microstructural and phylogenetic analyses to investigate whether the new samples belong to Physa acuta. The shell morphology, male copulatory complex and radula microstructure of the new material agree with those of the sewage snail. The molecular phylogenetic analyses using the cytochrome c oxidase subunit 1 (COI) gene confirmed morphological identification. We suggest to take prompt measures to prevent the expansion of Physa acuta in Llanquihue Lake or nearby aquatic ecosystems.

Resumo em Inglês:

Abstract Spatial variation in the environment may be an important source of morphological variation in many organisms. Tadpoles are valuable model organisms for studies of morphological variation, and in particular, the evaluation of the relationship between environmental and morphological variables. In heterogeneous environments, such as the temporary ponds found in the Cerrado savanna of central Brazil, understanding how environmental variables influence the morphological variation found in tadpole populations can provide important insights into this phenomenon. The present study thus aimed to (i) describe the morphometric variation in tadpoles found in different populations of Dendropsophus nanus and Dendropsophus minutus in the Cerrado of southern Maranhão state (Brazil), and (ii) relate this variation in tadpole morphology to the characteristics of the local ponds. Tadpoles were collected from 11 pounds in southern Maranhão, and the morphological space among the different populations was compared using NPMANOVA, separately for each species. The degree of association between the environmental and morphological matrices was then tested using a Stepwise Multiple Linear Regression. The morphological (tadpole morphometry) and environmental (pond characteristics) data matrices were obtained by ordination techniques. Considerable morphometric differences were found among populations in both species. In D. nanus, the morphometric variation was correlated with that of the substrate, whereas in D. minutus, morphometry was associated with the vegetation found in the pond. Overall, then, the study demonstrated that distinct environmental variables influenced significantly the morphometry of the tadpoles of each frog species.

Resumo em Inglês:

Abstract A new coenagrionid genus, Negragrion, is described for N. sagma sp.n. found in Argentina and Brazil (Holotype and allotype, pair in tandem. Argentina: Corrientes: Santo Tomé, arroyo Ita Cuá sobre RP 94, 28°26’48.30”S 56°00’33.11”W, 24.ii.2003, Muzón & Pessacq coll., MLP). The new genus is characterized by the shape of male cerci (decumbent from base; saddle-shaped; in lateral view with an acute apophysis directed dorsally located at 0.3 from base). The presence of a vulvar spine on S8 of females places this genus within Ischnurinae. A cladistics analysis using morphological data was carried out to determine its phylogenetic position. Negragrion gen.n. is recovered within the clade 4 as the sister group of the clade (Acanthallagma Acanthagrion (Oxyagrion, Fluminagrion)).

Resumo em Inglês:

Abstract We describe a new dipsadine snake species, of the genus Oxyrhopus Wagler, 1830, from the highlands of Bolivia. Oxyrhopus emberti sp. n. is diagnosed from its congeners based on external and hemipenial morphology. The new species inhabits the humid forests of Yungas and Tucumano-Bolivian Forest highlands, between 1.200 – 1.800 meters above sea level, and is likely to be a Bolivian endemic. We also discuss the relationships of the new species with Andean congeners and provide a key to the identification of the Oxyrhopus species from the Central Andes of Bolivia and Peru.

Resumo em Inglês:

Abstract The aim of this paper is to investigate the ultrastructural events that occur during pollen grains development, with emphasis in pollen grain wall and tapetum ontogeny in Ziziphus jujuba, Z. mucronata, Paliurus spina-christi (Paliureae) and Gouania ulmifolia (Gouanieae). Anthers at different developmental stages were processed according to classic techniques for transmission electron microscopy. Differences in the number of endothecium layers and in the number of tapetal cell nuclei were found. Tapetal cells present an anastomosing tubular network and large vesicles with fibrillar content in the cytoplasm. Pollen grain development and ontogeny of pollen grain wall are similar in the four species. The number of endothecium layers, the number of nuclei of the tapetal cells and tapetal cells ultrastructure of the four species support the phylogenetic relationships previously published for the Rhamnaceae family. Tapetal vesicles with fibrillar or polysaccharide content seem to be an exclusive characteristic of the tribes Paliureae and Gouanieae. Some ultrastructural characters of the pollen grain wall development are common to other species of Rhamnaceae, such as the primexine matrix present at the microspore mother cell stage, the aperture entirely built up during the tetrad stage, the thick and fibrillar intine, and the granular infractectum.

Resumo em Inglês:

Abstract The aim of this study was to evaluate the effect of phosphorus (P) on the toxicity of zinc (Zn) for the alga Raphidocelis subcapitata. P was provided in three concentrations: 2.3 x 10-4 mol L-1, 2.3 x 10-6 mol L−1 and 1.0 x 10-6 mol L−1. Algal cells were acclimated to the specific P concentrations before the start of the experiment. The chemical equilibrium software MINEQL+ 4.61 was employed to calculate the Zn2+ concentration. After acclimated, the algal cells were inoculated into media containing different Zn concentrations (0.09 x 10-6 mol L-1 to 9.08 x 10-6 mol L-1). The study showed that besides the reduction in algal growth rates, phosphorus had an important influence on the toxicity of zinc for microalga. The inhibitory Zn2+ concentration values for R. subcapitata were 2.74 x 10-6 mol L-1, 0.58 x 10-6 mol L-1 and 0.24 x 10-6 mol L-1 for the microalgae acclimated at P concentrations of 2.3 x 10-4 mol L-1, 2.3 x 10-6 mol L-1 and 1.0 x 10-6 mol L-1, respectively. Ecotoxicological studies should consider the interaction between metal concentrations and varying P values to provide realistic data of what occurs in phytoplankton communities in environments.

Resumo em Inglês:

Abstract Differences in species composition between sites (β diversity) may be the result of spatial species replacement (turnover) or nestedness (subgroups of species from a more diverse site). In fragmented landscapes, the environmental factors that lead to these differences may be spatially structured. Herein, our objective is to determine if the β diversity of Chrysomelidae (Coleoptera) is due to turnover or nestedness and whether the observed pattern is due to loss of forest cover or spatial processes in forest remnants immersed in a matrix dominated by intense agricultural practice. We used an incidence matrix of 99 species sampled from 16 forest remnants and found that the difference in species composition among the fragments is mostly determined by turnover and that this variation is not explained by forest cover or spatial variables. In regions where high habitat loss has generated landscapes containing small and islated forest fragments, structural features, related both to habitat (area, isolation, shape, etc.) and landscape (land use, landscape heterogeneity, etc.) could predict diversity patterns.

Resumo em Inglês:

Abstract Breast and cervical cancer represent a major problem of women’s global public health. Here, we investigated the chemical composition of essential oils from leaves and flowers of T. ostenii and the antineoplastic potential in a cervical cancer and breast cancer cell line, SiHa and MCF-7; and non-tumoral cells, HaCat. The chemical analysis revealed a predominance of oxygenated monoterpenes in both essential oils. The IC50 after 24 h of treatment was 72 ng/mL for EO 1 and 83 ng/mL for EO 2 in SiHa cells. For MCF-7 the IC50 was 174.3 ng/mL for EO 1. For HaCat cells it was 54.45 ng/mL for EO 1 and 20.83 ng/mL for EO 2. A synergistic effect with both essential oils and cisplatin was also verified. SiHa cells had their wound healing capacity reduced after 48 h of treatment with EO 2 and both essential oils were able to significantly inhibit the adhesion process and clonogenic ability after 24 h of treatment. Our results suggest a long-lasting inhibitory activity in SiHa cells because of the low recovery capacity of cells after treatment withdrawal. Flow cytometer with annexin V/propidium iodide demonstrated a majority cell death through late apoptosis after 24, 48 and 72 hours of treatment.

Resumo em Inglês:

Abstract Ecological restoration is a traditional option for recovering biodiversity and ecosystem functions. Birds perform pollination, seed dispersal, and pest-control services, which catalyze increases in habitat structure. Habitat complexity changes bird composition, but there is little evidence of its effects on bird functional diversity in Neotropical restorations. We tested whether bird functional diversity and composition respond to increased habitat complexity. Point-counts were performed (January-December 2015) in an area undergoing restoration (536 ha) in the Atlantic Forest of southeastern Brazil, in restorations with less and more structured vegetation and pastures and forest-fragments. The functional bird traits considered were diet, habitat, biomass, environmental sensitivity, and foraging strata. Increased habitat complexity was evaluated using plant characteristics (exotic grass, canopy, herbaceous cover, and diameter at breast height). A total of 172 bird species (5% endemic; 12% migratory) were recorded. Increased vegetation structure in both restored sites and forest-fragments drove a reorganization and addition of functional bird traits, which positively influenced functional richness, dispersion, and evenness. Shifts in plant-characteristics rearranged bird functional traits (diet-forest-dependence and diet-strata-foraging). The rapid development of vegetation structure is a key factor for restoration because it provides additional habitat for semi-dependent forest birds and enhances resilience and sustainability in new man-made forests.

Resumo em Inglês:

Abstract The first description of the fourth instar larva and pupa of Palpomyia mapuche Spinelli, Grogan & Ronderos and the pupa of P. subfuscula Ingram & Macfie are provided, as well as the redescription of the pupa of P. subaspera (Coquillett). Studied specimens were collected in lotic environments of Argentinian Patagonia, in Neuquén and Chubut Provinces. The described stages were examined and illustrated with a phase-contrast microscope. The larva was examined using a scanning electron microscope. Data on the bionomics for P. mapuche and new records for the three species are provided.

Resumo em Inglês:

Abstract The Caatinga’s black snake Boiruna sertaneja is a Pseudoboini species, endemic of the Caatinga biome. It is rare and fits in five traits that suggest it deserve more attention in reproduction research and conservation policies. Here we provide information on reproductive biology of B. sertaneja by adding new data about court and copula, fertility, nest, eggs and hatchling morphometry and pattern of color based on captivity specimens. The court and copula, as well as oviposition of B. sertaneja in the Caatinga are associated to period of high temperature and dry season. Our findings indicate that females of B. sertaneja, in nature, could select protected places or actively build their nests. We recorded three oviposture after a single event of copula by the female of B. sertaneja. The long time gap between copula and clutches strongly suggests that female B. sertaneja can store sperm in their oviducts for long periods or do facultative parthenogenesis. Clutch size and hatchling size of B. sertaneja was high. We observed variation on the pattern of coloration among hatchlings of same litter. This study comprises the first description of important aspects of reproduction and can give us some clues about how B. sertaneja reproduce in nature.