ABSTRACT This study evaluated the effects of including Moringa oleifera (moringa) leaf meal on performance, carcass yield and characteristics, and relative organ weights of broilers from 10 to 42 days of age. We distributed 420 male Cobb 500 chicks in a completely randomized design with five treatments and six replicates with 14 birds each. Treatments consisted of five experimental diets in which the moringa leaf meal was included at 0, 1.5, 3.0, 4.5, and 6.0% in the diets. The following performance variables were evaluated: weight gain, feed intake, and feed conversion; weights and yields of carcass, carcass traits, organs, and abdominal fat; and feet color. Significant differences between the treatment means were analyzed by Dunnett’s test. The meal inclusion levels did not affect performance, carcass characteristics and yield, or organs weight. However, feet pigmentation increased linearly, and abdominal fat was greater only at the 1.5% level when compared with the control group. Moringa leaf meal can be included in the diet of broilers from 10 to 42 days of age, up to the level of 6%, without compromising performance or carcass yield of these birds.

Abstract This study was undertaken to investigate the performance, carcass characteristics, and economic viability of diets including pasta waste in meal form (PWM) for quail. A total of 450 unsexed meat quail (Coturnix coturnix coturnix) were distributed into five treatments (0, 10, 20, 30, and 40% inclusion of PWM) in a completely randomized design with six replicates, each with 15 birds. The following rearing periods were evaluated: Phase 1 - 1 to 21 days; and period of 1 to 42 days. In Phase 1, the PWM levels did not influence feed intake (FI), and the PWM inclusion levels for optimal weight gain (WG) and feed conversion (FC) were estimated at 20.70% and 22.8%, respectively. In the cumulative period of 1 to 42 days, the PWM levels did not lead to differences in FI, WG, or FC. The yields of carcass and cuts were not influenced by the treatments; however, abdominal fat yield decreased as the PWM levels in the diets were increased. Economic analysis showed a reduction of the feeding cost in the studied phases. Pasta waste meal can be included up to the level of 40% without incurring losses in the performance or carcass yield of meat quail.

ABSTRACT The objective of this study was to determine the nutritional and energy compositions of cottonseed meal (CM), with or without enzyme supplementation, for broilers at different ages. A total of 672 male Cobb 500 chickens were distributed into four metabolism and ileal-digestibility trials. The experimental design was completely randomized, with four treatments and six replicates. The following treatments were tested: a corn- and soybean meal-based control diet without enzymes; control diet with enzyme addition; control diet with 25% replaced by CM; and control diet with enzyme addition and 25% replaced by CM. The following variables were investigated: apparent metabolizable energy (AME); nitrogen-corrected AME (AMEn); apparent metabolizability coefficients of dry matter, gross energy, phosphorus, and calcium; apparent ileal digestibility coefficients of dry matter, crude protein, calcium, and phosphorus; and digestible protein of CM. Enzyme supplementation did not affect the energy values of CM. The average values obtained in the pre-starter, starter, grower, and finisher phases were 2,958; 2,554; 1,676, and 1,963 kcal kg-1 for AME and 2,519; 2,282; 1,423, and 1,680 kcal kg-1 for AMEn, respectively. Enzyme addition improves the apparent digestibility coefficients of phosphorus and calcium of cottonseed meal in the grower phase. However, enzyme supplementation does not affect the ileal digestibility coefficients of these ingredients in broilers.

ABSTRACT The objective of this study was to evaluate the effect of including whole corn germ (WCG) on the performance; diet metabolizability; yields of carcass, cuts, and offal; and quality of meat of broilers. A total of 648 chicks were assigned to six treatments in a completely randomized design with six replicates, with 18 birds in each. Treatments consisted of a corn- and soybean meal-based control diet (0 g kg−1 WCG) and five test diets including WCG at the levels of 40, 80, 120, 160, and 200 g kg−1. Birds and diets were weighed at each seven days to determine feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR). The partial collection methodology was employed to determine the apparent metabolizable energy (AME), nitrogen-corrected AME (AMEn), and the apparent metabolizability coefficients of gross energy (AMCGE), dry matter (AMCDM), crude protein (AMCCP), and ether extract (AMCEE) of the diets. In the evaluation of meat quality, we analyzed the pH, cooking losses, shear force, water-holding capacity, color, and peroxide index of the meat. There was a difference for BWG and FCR in the total rearing period (1 to 42 days), for which optimum BWG was estimated as 2921 g/bird, with 118 g kg−1 inclusion of WCG. There was no difference for the AME, AMEn, and AMCCP of the diets, although AMCGE, AMCDM, and AMCEE declined as WCG was included. The increasing levels of WCG did not influence the yields of carcass and cuts or the meat quality. There was an increase in the yield of gizzard and proventriculus. Whole corn germ can be used at low levels in the diet of broilers without compromising their productive rates.

ABSTRACT The objective of this study was to evaluate levels of inclusion of an enzyme complex (EC) in corn- and soybean meal-based diets for laying hens on the digestibility of nutrients and energy. A metabolism trial was conducted using 75 Dekalb Brown laying hens at 26 weeks of age, which were distributed into five treatments with five replicates in a completely randomized design. The ingredients used in the diets received an additional (enriched) 3% methionine, lysine, cysteine, threonine, tryptophan, and metabolizable energy and 33.3% phosphorus. Treatments consisted of diets including 0, 150, 200, 250, or 300 mg.kg‒1 EC. We determined apparent metabolizable energy (AME); nitrogen-corrected AME (AMEn); apparent metabolizability coefficients of dry matter (DMAM), crude protein (CPAM), gross energy (GEAM), and phosphorus (PAM); digestible crude protein (CPD); and intake, retention, and excretion of phosphorus. No effects of EC levels were detected on the metabolizable energy values or digestibility coefficients, except for CPAM, which showed a quadratic response (maximum coefficient at 89.0 mg.kg‒1EC). A quadratic effect was also observed for CPD (minimum at 115 mg.kg‒1 EC), P excretion (maximum at 173.2 mg.kg‒1 EC), and P retention (maximum at 122.4 mg.kg‒1 EC) when EC was used. The use of the EC in corn- and soybean meal-based diets for laying hens improves their ileal digestibility of protein and apparent digestibility of phosphorus. However, EC addition to the diets does not affect AME, AMEn, or their metabolizability coefficients.

RESUMO Objetivou-se determinar a composição aminoacídica e os valores de proteína digestível de diferentes leveduras de cana-de-açúcar, bem como gerar equações de predição dos coeficientes de digestibilidade aparente da matéria seca (CDAMS) e proteína bruta (CDAPB), com base na composição química da levedura e idade dos frangos de corte. Quatro ensaios de digestilidade ileal foram realizados com 690 frangos de corte, divididos nas fases pré-inicial, inicial, crescimento e final. As aves foram alojadas em gaiolas de metabolismo e distribuídas num delineamento inteiramente casualizado constituído de cinco tratamentos e seis repetições de: oito, seis, cinco e quatro, respectivamente, para cada fase experimental. Os tratamentos foram: uma ração referência e quatro rações testes com a substituição de 30% da ração referência por quatro diferentes leveduras. As leveduras dois e quatro apresentaram maiores percentagem de aminoácidos. Entretanto, a levedura dois apresentou os maiores percentuais de CDAMS, CDAPB e de proteína digestível, estes sendo: 83,64; 81,22 e 14,66% na fase pré-inicial, 78,09; 78,28; 14,13% na inicial, 76,74; 74,75 e 13,49% na fase de crescimento e 72,98; 71,21 e 12,85% na fase final, respectivamente. Houve redução média de 0,424% no CDAMS e 0,428% no CDAPB, para cada dia de envelhecimento das aves. A partir da composição das leveduras e idade dos frangos, foram geradas as seguintes equações de predição: CDAMS = 77,4 - 0,424*(idade) - 7,51*(MS) -45,01*(EE) e - 0,424* (idade) + 3,02*(PB) + 126,93*(EE) - 0,03*(DGM) e CDAPB = 606,9 - 0,428*(idade) - 5,8*(MS) - 0,01*(DGM) e - 0,426*(idade) + 2,68(PB) + 139,7*(EE) - 0,03*(DGM).

ABSTRACT The aim of this study was to determine the amino acid composition and digestible protein values of different sugarcane yeasts, as well as to generate prediction equations for the apparent digestibility coefficient of dry matter (ADCDM) and crude protein (ADCCP), based on the chemical composition of the yeast and the age of the broilers. Four ileal digestion trials were carried out with 690 broilers, divided into the pre-starter, starter, growth and final phases. The birds were housed in metabolic cages and distributed in a completely randomised design, consisting of five treatments and six replications of eight, six, five and four birds respectively in each experimental phase. The treatments consisted of one reference feed and four test feeds, substituting 30% of the reference feed with four different yeasts. Yeasts two and four showed a higher percentage of amino acids. However, yeast two had the highest percentages of ADCDM, ADCCP and digestible protein: 83.64, 81.22 and 14.66% in the pre-starter phase; 78.09, 78.28 and 14.13% in the starter phase; 76.74, 74.75 and 13.49% in the growth phase; and 72.98, 71.21 and 12.85% in the final phase respectively. There was an average reduction of 0.424% in ADCDM and 0.428% in ADCCP for each day the birds aged. The following prediction equations were generated from the composition of the yeasts and age of the chickens: ADCDM = 77.4 - 0.424*(age) - 7.51*(DM) - 45.01*(EE) and - 0.424*(age) + 3.02*(CP) + 126.93*(EE) - 0.03*(GMD) and ADCCP = 606.9-0.428*(age) -5.8*(DM) - 0.01*(GMD) and -0.426*(age) + 2.68(CP) + 139.7*(EE) - 0.03*(GMD).

ABSTRACT The objective of this study was to determine prediction equations to estimate the nitrogen-corrected apparent metabolizable energy (AMEn) values of animal meals used in broiler diets through meta-analysis. A bibliographic review was undertaken with studies conducted in Brazil from 2000 to 2016 to catalogue information on AMEn values and the following chemical elements in the composition of the feedstuffs: crude protein (CP), ether extract (EE), gross energy (GE), mineral matter (MM), calcium (Ca), and phosphorus (P). Groups were also catalogued and formed according to sex and age of birds. Chemical correlations were analyzed, and a multiple linear regression model with the stepwise procedure was used to examine the association between the variables, which were included in the equation as a function of their importance. High and significant correlation coefficients between the independent (GE, MM, CP, EE, Ca, and P) and dependent variable (AMEn) contribute to the understanding of variations in the energy values of these feedstuffs. According to the coefficients of determination, the best equations to estimate AMEn of poultry offal meal and meat and bone meal are AMEn = 6139 − 45.5 CP + 0.356 GE − 123.5 MM ( R 2 = 0.8302 ) and AMEn = 2267 + 19.9 CP + 67.9 EE − 44.4 MM ( R 2 = 0.9021 ) , respectively.

ABSTRACT The objective of this study was to determine the energy value of pasta waste through a metabolism trial and determine the best level of its inclusion in broiler diets. In the metabolism trial, sixty 14-day-old birds were assigned to two treatments (control diet and a diet in which 30% was replaced by the byproduct) with six replicates and five birds per experimental unit. In the performance trial, 525 one-day-old chicks were assigned to treatments consisting of five levels of pasta waste (0, 100, 200, 300, and 400 g kg−1) in the diet, with seven replicates and 15 birds per experimental unit, in a completely randomized design. The phases of up to 7, 21, 35, and 42 days of age were evaluated. At the end, two broilers with average weight were selected per plot for carcass evaluation. An economic analysis was undertaken. The calculated apparent metabolizable energy (AME) value of the waste was 3812 kcal kg−1, and its nitrogen-corrected AME was 3616 kcal kg−1. In the performance trial, no significant difference was detected from 1 to 7 days. However, in the other phases, a decreasing effect was observed on feed intake, weight gain, slaughter weight, hot- and cold-carcass weights, empty- and full-gizzard weights and yields, cuts (chest, drumstick, thigh, wings, and back), and feed conversion worsened. The revenue and gross margin calculated for the diets decreased with pasta waste. Therefore, pasta waste is not a viable alternative, except in the pre-starter phase of broilers.

ABSTRACT A total of 450 one-day-old male broiler chicks were used to evaluate the effect of the sugarcane yeast on performance, body composition, and development of the intestinal mucosa. The experiment was carried out in a completely randomized design with five treatments and six replicates of 15 birds. Sugarcane yeast was included in the experimental diets at the levels of 0, 12.5, 25.0, 37.5, and 50.0 g kg−1. Body weight, weight gain, feed intake, and feed conversion were determined. At the end of the experiment, four broiler chicks were slaughtered per experimental unit: two were used for evaluation of moisture content, crude protein, fat, and body ash and the other two were used in the collection of small-intestine segments for evaluation of villus height and crypt depth. The increasing yeast levels resulted in a linear increase in feed intake and feed conversion. Inclusion of more than 14.4 g kg−1 yeast resulted in a reduction of body ash content. Villus height and crypt depth in the jejunum showed maximum values at the sugarcane yeast levels of 20.9 and 20.6 g kg−1, respectively. In the ileum, the crypt depth reduction at the level of 25.6 g kg−1 also resulted in an increase in villus:crypt ratio. Yeast inclusion increases feed intake and feed conversion, improves body mineral absorption, and increases villus height in the jejunum and the villus:crypt ratio in the ileum of broiler chicks.

RESUMO Objetivou-se avaliar o efeito do nível de substituição do gérmen integral de milho (GIM) sobre os valores energéticos obtidos pelos métodos de Matterson e da regressão. Foram utilizadas 120 aves com 45 semanas de idade, da linhagem Embrapa 051. O ensaio foi delineado inteiramente ao acaso, com cinco tratamentos e seis repetições de 4 aves. Os tratamentos foram compostos pela ração referência (RR) e quatro níveis de substituição da RR (10, 20, 30 e 40% pelo GIM). Foi utilizado o método de coleta total de excretas. As variáveis analisadas foram: energia metabolizável aparente (EMA), aparente corrigida para o balanço de nitrogênio (EMAn), tempo (TP) e taxa de passagem (TxP) do GIM. A relação entre as variáveis e os níveis de GIM foi estudada por análise de regressão. O tempo e a taxa de passagem foram afetados linearmente pelo nível de substituição do GIM. Os valores de EMA e EMAn foram reduzidos com o aumento do nível de substituição até 31,4 e 32,2% de GIM, respectivamente. O método da regressão proporcionou melhores estimativas para EMA e EMAn do GIM. Os valores de EMA e EMAn do GIM para aves de postura foram 5,161 e 5,066 kcal g-1, respectivamente.

ABSTRACT The aim of this study was to evaluate the effect of the substitution level of corn germ meal (CGM) on energy values obtained with Matterson's method and regression analysis. One hundred twenty laying hens from Embrapa 051 strain with 45 weeks of age, were used. The trial design was completely randomised, with five treatments and six replications of four birds. The treatments consisted of the reference feed (RF) and four other diets with dietary substitution levels of RF by CGM (10, 20, 30 and 40%). The method of total excreta collection was used. The variables analysed were: apparent metabolizable energy (AME), AME corrected for nitrogen balance (AMEn), time (T) and passage rate (PR) of the CGM. The relationship between the variables and levels of CGM was studied with regression analysis. The time and passage rate were linearly affected by the level of substitution of the CGM; with increases in the level of substitution. The values for AME and AMEn were reduced by 31.4% and 32.2% CGM respectively. The regression method provides better estimates for AME and AMEn of the CGM. In laying hens the CGM values for AME and AMEn were 5.161 and 5.066 kcal g-1, respectively.

<title>Resumo</title><p>Este experimento foi conduzido com o objetivo de analisar o desempenho e características de carcaça de codornas europeias alimentadas com resíduo da agroindústria da goiaba em substituição ao milho. Foram utilizadas 140 aves, distribuídas em um delineamento inteiramente casualizado. Os tratamentos consistiram em uma ração controle e quatro rações com níveis de inclusão 2,5; 5,0; 7,5 e 10,0% de farelo de goiaba em uma ração à base de milho e farelo de soja. Não foram observadas diferenças significativas (P>0,05) para as variáveis: ganho de peso, consumo de ração, conversão alimentar, eficiência alimentar, peso e rendimento de carcaça e cortes nobres (peito, coxa e sobre-coxa) asas, dorso, pescoço cabeça, pés e órgãos comestíveis (coração, fígado e moela). O farelo de goiaba pode ser utilizado como ingrediente alternativo nas dietas de codornas europeias, no período de 16 a 38 dias de idade, até o nível de 10% de inclusão, sem deprimir o desempenho produtivo e rendimento de carcaças das aves.</p>

<title>Abstract:</title><p>This experiment was conducted to analyze the performance and carcass characteristics of European quail fed agroindustry residue of guava in substitution of corn. 140 birds were used, distributed in a completely randomized design. The treatments consisted of a control diet and four diets with levels of guava waste inclusion (2.5, 5.0, 7.5 and 10.0%) to a diet based on corn and soybean meal. There were no significant differences (P> 0.05) for the variables: weight gain, feed intake, feed conversion, feed efficiency, weight and carcass yield and prime cuts (breast, drumstick and thigh) wings, back, neck, head, feet and foodstuffs organs (heart, liver and gizzard). The guava waste can be used as alternative ingredient in the diets of European quail in the period of 16-38 days of age, up to the level of 10% inclusion without depressing the performance and yield of poultry carcasses</p>

An outstanding feature of poultry production that provides animal protein yield for human feeding is its short production cycle. This characteristic has a linear relationship with waste production. Increasing the inclusion of this residue in diets in the near future is desirable in step with the growth of poultry production since it offers a better environmental and nutritional alternative to current methods. We evaluated the effects on the performance and carcass characteristics of broiler chickens produced by the inclusion of poultry offal meal (POM) in their feed. Treatments consisted of a control diet (corn, Zea mays and soybean, Glycine max) and four diets with inclusion of 30, 60, 90 and 120 g kg-1 of POM. The diets were formulated based on the level of digestible amino acid once categorized as isocalcic, isophosphoric, isosodic, isoenergetic and isonutritive for protein, methionine+cystine, lysine and threonine. The feed's electrolytes were corrected so that each diet had the same electrolytic balance. The variables analyzed were feed intake, weight gain, feed conversion ratio, body weight, carcass yield, chicken cut yield and abdominal fat. Feed intake was not affected by the quantities of POM added. The weight gain, feed conversion, carcass yield and noble cuts presented quadratic responses to the treatments. Abdominal fat increased linearly. The performance of the poultry, and carcass characteristics were maximized by the inclusion of 53 and 65 g kg-1, respectively, of POM in the diet, and the inclusion of 120 g kg-1 of POM provided greater disposition of abdominal fat.

The zoonotic potential to cause human and/or animal infections among multidrug-resistant extraintestinal pathogenic Escherichia coli from avian origin was investigated. Twenty-seven extraintestinal pathogenic E. coli isolates containing the increased survival gene (iss) were obtained from the livers of healthy and diseased poultry carcasses at two slaughterhouses in Salvador, northeastern Brazil. The antimicrobial resistance-susceptibility profiles were conducted with antibiotics of avian and/or human use by the standardized disc-diffusion method. Antimicrobial resistance was higher for levofloxacin (51.8%), amoxicillin/clavulanic acid (70.4%), ampicillin (81.5%), cefalotin (88.8%), tetracycline (100%) and streptomycin (100%). The minimum inhibitory concentrations above the resistance breakpoints of doxycycline, neomycin, oxytetracycline and enrofloxacin reached, respectively, 88.0%, 100%, 75% and 91.7% of the isolates. Strains with high and low antimicrobial resistance were i.p. administered to Swiss mice, and histopathological examination was carried out seven days after infection. Resistance to goat and human serum complement was also evaluated. The results show that Swiss mice challenged with strain 2B (resistant to 11 antimicrobials) provoked a severe degeneration of hepatocytes besides lymphocytic infiltration in the liver, whereas the spleen showed areas of degeneration of the white and red pulp. Conversely, the spleen and liver of mice challenged with strain 4A (resistant to two antimicrobials) were morphologically preserved. In addition, complement resistance to goat and human serum was high for strain 2B and low for strain 4A. Our data show that multidrug resistance and pathogenesis can be correlated in extraintestinal pathogenic E. coli strains obtained from apparently healthy poultry carcasses, increasing the risk for human public healthy.

The objective of this study was to evaluate the requirement of digestible tryptophan for white laying hens in the production stage fed diets of different digestible tryptophan:digestible lysine ratios, as well as animal performance and histological alterations in their reproductive and digestive systems. A total of 280 white laying hens at 29 weeks of age were distributed in a completely randomized design with five treatments and seven replications with eight birds in each. The treatments consisted of a base feed, formulated with corn, soybean meal and corn gluten meal, and supplemented with the synthetic amino acids L-lysine, DL-methionine, L-threonine, L-isoleucine, L-arginine, and L-valine, so as to meet the nutritional requirements for laying hens, except for digestible tryptophan. The basal diet was supplemented with 0.00; 0.017; 0.035; 0.052; and 0.069 g/kg of L-tryptophan in substitution for corn starch with the objective of reaching the levels of 0.151; 0.167; 0.183; 0.199; and 0.215 g/kg of digestible tryptophan in the feed. For the ratio between digestible amino acids and lysine, the recommendation of Brazilian Tables for Poultry and Swine was followed, except for the digestible tryptophan:digestible lysine ratios, which were 19, 21, 23, 25 and 27 for each treatment. The variation in the digestible tryptophan:digestible lysine ratio promoted changes in performance and in the histological characteristics, improving the results. The digestible tryptophan:digestible lysine ratio of 24.5% in the feed of white laying hens in production stage promotes better animal performance and histological results.

O objetivo do estudo foi avaliar a digestibilidade do farelo de algodão com ou sem adição de enzimas (fitase e protease) para suínos em crescimento. Foram utilizados 18 leitões machos castrados, alojados em gaiolas de metabolismo, distribuídos em delineamento inteiramente casualizado com padronização do peso vivo (PV) com média de 25,8 ± 3,6 kg, com três tratamentos e seis repetições. Os tratamentos consistiram em uma dieta referência à base de milho e farelo de soja, o segundo tratamento com substituição de 30% da dieta referência por farelo de algodão sem enzimas, e o terceiro com substituição de 30% da dieta referência por farelo de algodão com adição de enzimas. Foi determinada a proteína digestível, energia digestível, coeficiente de digestibilidade da matéria seca, da energia e da proteína. Também foi observado o balanço de nitrogênio e fósforo. O uso do farelo de algodão com adição de enzimas em dietas para suínos em crescimento, não afetou os coeficientes de digestibilidade da matéria seca, energia bruta e proteína bruta; porém contribuíram com a redução do fósforo excretado nas fezes e melhorou sua absorção. Não foi observado melhora do balanço de nitrogênio nas dietas contendo farelo de algodão com enzimas.

This study evaluated the digestibility of cottonseed meal with or without addition of enzymes (phytase and protease) for growing pigs. It was used 18 barrows, housed in metabolism cages, distributed in a completely randomized design, standardizing body weight (bw) with average of 25.8 ± 3.6 kg, with three treatments and six repetitions. The treatments consisted of a reference diet based on corn and soybean meal, the second treatment with replacement of 30% of the reference diet by cottonseed meal without enzymes, and the third with 30% of the reference diet replaced by cottonseed meal with added enzymes. Was determined the digestible protein, digestible energy, digestibility of dry matter, energy and protein. It was also registered the balance of nitrogen and phosphorus. The use of cottonseed meal with the addition of enzymes in diets for growing pigs has no effect on the digestibility of dry matter, gross energy and crude protein, but improved the absorption of phosphorus, consequently reducing its excretion in the feces. There was no improvement in nitrogen balance in the diets containing cottonseed meal with enzymes.