Brazilian Dental Journalhttps://www.scielo.br/feed/bdj/2017.v28n3/2024-03-19T20:31:31.592000ZVol. 28 No. 3 - 2017WerkzeugGenetic Polymorphism in MMP9 May Be Associated With Anterior Open Bite in Children10.1590/0103-64402016009922024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZKüchler, Erika CalvanoBarreiros, DrielySilva, Raphaela Oliveira daAbreu, Júlia Guimarães Barcellos deTeixeira, Ellen CardosoSilva, Raquel Assed Bezerra daSilva, Lea Assed Bezerra daNelson, PauloRomano, Fábio LourençoGranjeiro, José MauroAntunes, Lívia Azeredo AlvesAntunes, Leonardo Santos
<em>Küchler, Erika Calvano</em>;
<em>Barreiros, Driely</em>;
<em>Silva, Raphaela Oliveira Da</em>;
<em>Abreu, Júlia Guimarães Barcellos De</em>;
<em>Teixeira, Ellen Cardoso</em>;
<em>Silva, Raquel Assed Bezerra Da</em>;
<em>Silva, Lea Assed Bezerra Da</em>;
<em>Nelson, Paulo</em>;
<em>Romano, Fábio Lourenço</em>;
<em>Granjeiro, José Mauro</em>;
<em>Antunes, Lívia Azeredo Alves</em>;
<em>Antunes, Leonardo Santos</em>;
<br/><br/>
Abstract Anterior open bite (AOB) has a multifactorial etiology caused by the interaction of sucking habits and genetic factors. The aim of this study was to evaluate the association between AOB and polymorphisms in genes that encode Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). Four hundred and seventy-two children that presented at least one sucking habit were evaluated. Children were examined clinically for the presence of AOB. Genomic DNA was extracted from saliva. Genotyping of the selected polymorphisms in MMP2, MMP3, MMP9, TIMP1 and TIMP2 was carried out by real-time PCR using the TaqMan method. Allele and genotype frequencies were compared between the groups with and without AOB using the PLINK® software in a free and in a recessive model using a chi-square test. Logistic regression analysis was implemented (p≤0.05). Two hundred nineteen children had AOB while 253 did not. The polymorphism rs17576 in MMP9 was significantly associated with AOB (p=0.009). In a recessive model GG genotype was a protective factor for AOB (p=0.014; OR 4.6, 95%CI 1.3-16.2). In the logistic regression analysis, none of the genes was associated with AOB. In conclusion, the polymorphism rs17576 (glutamine for arginine substitution) in MMP9 was a protective factor for AOB.TLR2 Signaling Pathway in Alveolar Bone Osteogenesis Induced by <i>Aloe vera</i> and Xenograft (XCB)10.1590/0103-64402016008342024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZKresnoadi, UtariRahayu, Retno PudjiRubianto, MSudarmo, Subijanto MartoBudi, Hendrik Setia
<em>Kresnoadi, Utari</em>;
<em>Rahayu, Retno Pudji</em>;
<em>Rubianto, M</em>;
<em>Sudarmo, Subijanto Marto</em>;
<em>Budi, Hendrik Setia</em>;
<br/><br/>
Abstract The aim of this study was to find the role of TLR2 signaling pathway in reducing osteoclast activity and promoting osteoblast growth by inducing a combination of Aloe vera and cancellous bovine xenograft (XCB) into dental extraction socket. Forty-eight Cavia cobayas were used. They were divided into eight groups (n=6). For control group, their mandibular incisors were extracted and filled with PEG. For treatment groups, they were extracted and filled with XCB, Aloe vera and the combination of Aloe vera and XCB. The first four groups were sacrificed after 7 days and the other groups after 30 days. Immunohistochemistry and histopathology examination were conducted to examine TLR2, TNFa, OPG, collagen-1, and the osteoblast and osteoclast expressions. The expressions of TLR2, OPG and Collagen-1, as well as the number of osteoblast were increased. Meanwhile, the expressions of TNFa and osteoclast were decreased. The study finding was that TLR2 signaling pathway influenced alveolar bone osteogenesis process by reducing osteoclast activity and stimulating osteoblast growth induced by the combination of Aloe vera and XCB.Repair of Bone Defects with Chitosan-Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement10.1590/0103-64402016014542024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZMoraes, Paola Castro deMarques, Isabela Cristina de SouzaBasso, Fernanda GonçalvesRossetto, Hebert LuisPires-de-Souza, Fernanda de Carvalho PanzeriCosta, Carlos Alberto de SouzaGarcia, Lucas da Fonseca Roberti
<em>Moraes, Paola Castro De</em>;
<em>Marques, Isabela Cristina De Souza</em>;
<em>Basso, Fernanda Gonçalves</em>;
<em>Rossetto, Hebert Luis</em>;
<em>Pires-De-Souza, Fernanda De Carvalho Panzeri</em>;
<em>Costa, Carlos Alberto De Souza</em>;
<em>Garcia, Lucas Da Fonseca Roberti</em>;
<br/><br/>
Abstract Innovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. The purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. Eighteen New Zealand white rabbits (Oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). Four bone defects were created in the rabbits calvaria, which were individually filled with the biomembrane, scaffold, blood clot (negative control) and autologous bone (positive control). Histopathological analysis was performed using optical microscope at 32´, 64´, 125´ and 320´ magnifications. Cell response to inflammation and new bone tissue formation was quantified using a score system. The biomembrane group presented greater inflammatory response at 15 days, with significant difference to autologous bone group (p<0.05). There was no statistically significant difference for foreign body type reaction among groups (p>0.05). Concerning new bone formation, linear closure of the defect area was observed more evidently in the group with autologous bone. The scaffold group presented similar results compared with the autologous bone group at 30 days (p>0.05). Both tested biomaterials presented similar biocompatibility compared with the control groups. In addition, the biomembrane and scaffold presented similar osteoinductive capacity, stimulating bone repair process in the course of the experimental time intervals.RANK/RANKL/OPG Expression in Rapid Maxillary Expansion10.1590/0103-64402016011162024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZArnez, Maya Fernanda ManfrinRibeiro, Larissa Soares NogueiraBarretto, Gabriel DessottiMonteiro, Patrícia MariaErvolino, EdilsonStuani, Maria Bernadete Sasso
<em>Arnez, Maya Fernanda Manfrin</em>;
<em>Ribeiro, Larissa Soares Nogueira</em>;
<em>Barretto, Gabriel Dessotti</em>;
<em>Monteiro, Patrícia Maria</em>;
<em>Ervolino, Edilson</em>;
<em>Stuani, Maria Bernadete Sasso</em>;
<br/><br/>
Abstract The aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (a=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME.Demographic and Clinical Profile of Oral Squamous Cell Carcinoma from a Service-Based Population10.1590/0103-64402016012572024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZAlves, Alessandro MennaCorrea, Marcos BrittoSilva, Karine Duarte daAraújo, Lenita Maria Aver deVasconcelos, Ana Carolina UchoaGomes, Ana Paula NeutzlingEtges, AdrianaTarquinio, Sandra Beatriz Chaves
<em>Alves, Alessandro Menna</em>;
<em>Correa, Marcos Britto</em>;
<em>Silva, Karine Duarte Da</em>;
<em>Araújo, Lenita Maria Aver De</em>;
<em>Vasconcelos, Ana Carolina Uchoa</em>;
<em>Gomes, Ana Paula Neutzling</em>;
<em>Etges, Adriana</em>;
<em>Tarquinio, Sandra Beatriz Chaves</em>;
<br/><br/>
Abstract The aim of this study was to evaluate the demographic and clinical profile of the oral squamous cells carcinoma (OSCC) cases registered in a center of oral diagnosis in southern Brazil. Eight hundred and six individuals with OSCC from 1959 to 2012 were included in this study. The variables recorded were: sex, age, occupation according to workplace, alcohol and tobacco consumption, skin color, tumor location, histological type, clinical appearance, size, evolution time, presence of pain and lymph node metastasis. Descriptive analysis was performed and the associations between variables were assessed using the chi-square and Fisher’s exact test, with a confidence level of 5%. OSSC was more common in males (76.6%) aged between 51 to 70 years (53.9%). The most frequent sites were lower lip vermilion (23.3%), tongue (20.2%) and gingiva/alveolar ridge (18.1%). There was a strong association between outdoor occupation and white skin color with lip squamous cell carcinoma (LSCC). OSCC intraoral lesions were commonly more painful, larger than 2 cm and presented lymphatic metastasis. In conclusion, most of the results confirm the data from literature about sex, age, tumor location and occupation. Moreover, the positive correlations between LSCC and occupation, between LSCC and white skin color, and between bigger lesions and presence of pain/cervical metastasis also corroborate the literature data.Bioactive Glass Particles in Two-Dimensional and Three-Dimensional Osteogenic Cell Cultures10.1590/0103-64402016009532024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZAlves, Luciana B.Souza, Sérgio L. S. deTaba, MarioNovaes, Arthur B.Oliveira, Paulo T. dePalioto, Daniela B.
<em>Alves, Luciana B.</em>;
<em>Souza, Sérgio L. S. De</em>;
<em>Taba, Mario</em>;
<em>Novaes, Arthur B.</em>;
<em>Oliveira, Paulo T. De</em>;
<em>Palioto, Daniela B.</em>;
<br/><br/>
Abstract This study aimed to investigate the influence of a three-dimensional cell culture model and bioactive glass (BG) particles on the expression of osteoblastic phenotypes in rat calvaria osteogenic cells culture. Cells were seeded on two-dimensional (2D) and three-dimensional (3D) collagen with BG particles for up to 14 days. Cell viability and alkaline phosphatase (ALP) activity was performed. Cell morphology and immunolabeling of noncollagenous bone matrix proteins were assessed by epifluorescence and confocal microscopy. The expressions of osteogenic markers were analyzed using RT-PCR. Mineralized bone-like nodule formation was visualized by microscopy and calcium content was assessed quantitatively by alizarin red assay. Experimental cultures produced a growing cell viability rate up to 14 days. Although ALP activity at 7 days was higher on BG cultures, cells on 3D and 3D+BG had an activity decrease of ALP at 14 days. Three-dimensional conditions favored the immunolabeling for OPN and BSP and the expression of ALP and COL I mRNAs. BG particles influenced positively the OC and OPN mRNAs expression and calcified nodule formation in vitro. The results indicated that the 3D cultures and BG particles contribute to the expression of osteoblastic phenotype and to differentiated and mineralized matrix formation.Analyses of Biofilm on Implant Abutment Surfaces Coating with Diamond-Like Carbon and Biocompatibility10.1590/0103-64402016011362024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZHuacho, Patricia Milagros MaqueraNogueira, Marianne N. MarquesBasso, Fernanda G.Jafelicci, MiguelFrancisconi, Renata S.Spolidorio, Denise M. P.
<em>Huacho, Patricia Milagros Maquera</em>;
<em>Nogueira, Marianne N. Marques</em>;
<em>Basso, Fernanda G.</em>;
<em>Jafelicci, Miguel</em>;
<em>Francisconi, Renata S.</em>;
<em>Spolidorio, Denise M. P.</em>;
<br/><br/>
Abstract The aim of this study was to evaluate the surface free energy (SFE), wetting and surface properties as well as antimicrobial, adhesion and biocompatibility properties of diamond-like carbon (DLC)-coated surfaces. In addition, the leakage of Escherichia coli through the abutment-dental implant interface was also calculated. SFE was calculated from contact angle values; R a was measured before and after DLC coating. Antimicrobial and adhesion properties against E. coli and cytotoxicity of DLC with human keratinocytes (HaCaT) were evaluated. Further, the ability of DLC-coated surfaces to prevent the migration of E. coli into the external hexagonal implant interface was also evaluated. A sterile technique was used for the semi-quantitative polymerase chain reaction (semi-quantitative PCR). The surfaces showed slight decreases in cell viability (p<0.05), while the SFE, R a, bacterial adhesion, antimicrobial, and bacterial infiltration tests showed no statistically significant differences (p>0.05). It was concluded that DLC was shown to be a biocompatible material with mild cytotoxicity that did not show changes in R a, SFE, bacterial adhesion or antimicrobial properties and did not inhibit the infiltration of E. coli into the abutment-dental implant interface.Effect of Occlusal Splints on the Stress Distribution on the Temporomandibular Joint Disc10.1590/0103-64402016014592024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZFerreira, Fabiane MariaCézar, PauloSoares, Carlos JoséRamos, António Manuel de Amaral MonteiroFernandes-Neto, Alfredo Júlio
<em>Ferreira, Fabiane Maria</em>;
<em>Cézar, Paulo</em>;
<em>Soares, Carlos José</em>;
<em>Ramos, António Manuel De Amaral Monteiro</em>;
<em>Fernandes-Neto, Alfredo Júlio</em>;
<br/><br/>
Abstract Conservative approach, including occlusal splint therapy, is the first option to treat temporomandibular disorders (TMD), because of its reversibility. The present study analyzed the effect of the articular disc position and occlusal splints use on the stress distribution on this disc. A two-dimensional (2D) finite element (FE) model of the temporomandibular joint with the articular disc at its physiologic position was constructed based on cone-beam computed tomography. Three other FE models were created changing the disc position, according to occlusal splint use and anterior disc displacement condition. Structural stress distribution analysis was performed using Marc-Mentat package. The equivalent von Mises stress was used to compare the study factor. Higher stress concentration was observed on the intermediate to anterior zone of the disc, with maximum values over 2MPa. No relevant difference was verified on the stress distribution and magnitude comparing disc positions and occlusal splint use. However, there was stress reduction arising from the use of the occlusal splints in cases of anterior disc displacement. In conclusion, based on the generated FE models and established boundary conditions, the stress increased at the intermediate zone of the TMJ disc during physiological mandible closure. The stress magnitude was similar in all tested situationsSurvival/Success of Dental Implants with Acid-Etched Surfaces: A Retrospective Evaluation After 8 to 10 Years10.1590/0103-64402016014712024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZNicoli, Lélis GustavoOliveira, Guilherme José Pimentel Lopes deLopes, Beatriz Maria ValérioMarcantonio, CláudioZandim-Barcelos, Daniela LealMarcantonio, Elcio
<em>Nicoli, Lélis Gustavo</em>;
<em>Oliveira, Guilherme José Pimentel Lopes De</em>;
<em>Lopes, Beatriz Maria Valério</em>;
<em>Marcantonio, Cláudio</em>;
<em>Zandim-Barcelos, Daniela Leal</em>;
<em>Marcantonio, Elcio</em>;
<br/><br/>
Abstract The aim of this study was to conduct a retrospective evaluation of the survival and success rates of dental implants with acid-etched surfaces after 8-10 years of function. Forty-four patients who received 183 implants 8-10 years ago were evaluated. Clinical examinations were performed around the implants and natural teeth. The following parameters were measured: visible plaque index (VPI), marginal bleeding index (MBI), probing depth (PD), bleeding on probing (BOP) and clinical attachment level (CAL). To considerer an implant as a success case, the following criteria were considered: absence of peri-implant infection and suppuration, absence of implant mobility, absence of persistent pain and dysesthesia and absence of radiolucency around the implant. Overall, 178 implants were categorized as surviving (97.3%), 155 were categorized as successful (84.7%), 5 implants (2.7%) were lost (1 in the maxilla and 4 in the mandible), and 3 implants were not under functional load (2.0 %). 20 implants were diagnosed with peri-implantitis. Thus, the survival rate was 97% and the success rate was 85%. In conclusion, implants with acid-etched surfaces showed high survival and success rates after a period of 8 to 10 years of function.The In Situ Effect of Titanium Tetrafluoride Gel on Erosion/Abrasion Progression in Human Dentin10.1590/0103-64402016012472024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZMantilla, Taís FonsecaTurssi, Cecilia PedrosoRamos-Oliveira, Thayanne MonteiroSilva, Camila Vieira daSuzuki, Luis CláudioFreitas, Patricia Moreira de
<em>Mantilla, Taís Fonseca</em>;
<em>Turssi, Cecilia Pedroso</em>;
<em>Ramos-Oliveira, Thayanne Monteiro</em>;
<em>Silva, Camila Vieira Da</em>;
<em>Suzuki, Luis Cláudio</em>;
<em>Freitas, Patricia Moreira De</em>;
<br/><br/>
Abstract Erosion incidence is increasing and its control is still a challenge in clinical practice. This study evaluated 4% TiF4-gel effects on eroded human dentin subjected to in situ erosive/abrasive episodes. Seventy-two previously eroded dentin slabs (0.05 M citric acid, pH 2.3, 20 min) were allocated to 6 groups (n=12) according to the treatment to be performed during the in situ phase and number of erosive/abrasive cycles, as follows: 4% TiF4-gel applied once (TiF41), twice (TiF42) or three times (TiF43) followed by 1, 2 and 3 erosive/abrasive cycles, respectively. Gel was applied before the beginning of the next cycle. Control groups were subjected to 1 (C1), 2 (C2) and 3 (C3) erosive/abrasive cycles only. A seventh group (n=12) comprised in vitro uneroded samples (UN) subjected to 3 erosive/abrasive cycles. Each cycle corresponded to 2 days of erosive (citric acid 0.5%, pH 2.6, 6x/day) and abrasive (electric toothbrush, 10 s/sample, 1 x/day) challenges. Samples were evaluated under profilometry and environmental scanning electron microscopy (ESEM). Atomic force microscopy images (AFM) were also made (n=3). Repeated measures 2-way ANOVA and Tukey test (p<0.001) showed that TiF42, which did not differ from TiF41 and TiF43, revealed a significant reduction in surface loss compared to all control groups. TiF41 and TiF43 showed no significant difference from C1, but both groups demonstrated significantly smaller surface loss than C2 and C3. ESEM and AFM micrographs suggested alterations on treated surfaces compared to samples from control groups, showing reduced diameters of dentinal tubules lumens. Therefore, TiF4 was able to reduce the progression of erosive/abrasive lesions.Influence of the Extended Use of Desensitizing Toothpastes on Dentin Bonding, Microhardness and Roughness10.1590/0103-64402016012922024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZAguiar, Juliana DiasMedeiros, Igor StudartSouza, Mário Honorato Silva eLoretto, Sandro Cordeiro
<em>Aguiar, Juliana Dias</em>;
<em>Medeiros, Igor Studart</em>;
<em>Souza, Mário Honorato Silva E</em>;
<em>Loretto, Sandro Cordeiro</em>;
<br/><br/>
Abstract The aim of this study was to evaluate the influence of an extended use of desensitizing toothpastes (DTs) on dentin bonding, microhardness and roughness. One hundred and twenty bovine incisor teeth were randomly divided into four groups: G1, distilled water (WATER); G2, Colgate Total 12 (CT12); G3, Colgate Sensitive Pro-Relief (CSPR); and G4, Sensodyne Repair ?αμπ; Protect (SRP). Dentin surfaces were etched with 17% EDTA and 2 years of simulated tooth brushing (20,000 cycles) was performed on their surfaces. Knoop microhardness, surface roughness and scanning electron microscopy (SEM) were performed before and after the simulated tooth brushing. For microshear bonding test, a 2-step self-etching adhesive system (Clearfil SE Bond) was used and 0.8 mm diameter composite resin (Filtek Z350 XT) cylinders were built. Microshear test was performed with an orthodontic wire and with a crosshead speed of 0.5 mm/min. The data were analyzed for: 1) bond strength (one-way ANOVA), 2) microhardness intra-group (Student’s test) and inter-group (one-way ANOVA/Tukey’s test) comparisons, 3) roughness intra-group (Student’s test/Wilcoxon’s test) and inter-group (Kruskal-Wallis/Student-Newman-Keuls test) comparisons. The extended use of both dentifrices (conventional and for sensitive teeth) did not affect the bond strength and produced a significant increase in microhardness and roughness of the dentin, except for the microhardness of the SRP group. The simulated tooth brushing technique with water produced an increase in roughness, without reducing significantly the dentin microhardness.Characterization of Bis-Acryl Composite Resins for Provisional Restorations10.1590/0103-64402016014182024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZSchwantz, Júlia K.Oliveira-Ogliari, AlineMeereis, Carine T.Leal, Fernanda B.Ogliari, Fabrício A.Moraes, Rafael R.
<em>Schwantz, Júlia K.</em>;
<em>Oliveira-Ogliari, Aline</em>;
<em>Meereis, Carine T.</em>;
<em>Leal, Fernanda B.</em>;
<em>Ogliari, Fabrício A.</em>;
<em>Moraes, Rafael R.</em>;
<br/><br/>
Abstract This study investigated the in vitro performance of the commercial bis-acryl composite resins Systemp C&B II (SYS, Ivoclar Vivadent), Protemp 4 (PT4, 3M ESPE), Structur 2C (ST2, Voco) and ProviPlast (PVP, Biodinamica). Characterization involved optical (color stability, translucency parameter, fluorescence), surface (roughness, morphology and elemental composition), physical-chemical (viscosity, polymerization kinetics) and mechanical analyses (Poisson ratio, biaxial flexural strength, flexural modulus). Most tests were carried out after 24 h, but optical and mechanical analyses were carried out after storage in water at 37 °C for 1, 15, and 30 days. Data were statistically analyzed (a=0.05). Most results were material dependent. SYS and PT4 showed stability in color and translucency over time. All materials had similar or higher fluorescence than human enamel. SC2 and PVP showed rougher surfaces than the other bis-acryl composites. Smaller filler particles were observed on the surface of PT4 and PVP compared with the coarser particles from ST2 and SYS. Viscosity readings indicated a thixotropic behavior for all tested materials. SYS had the lowest and PT4 the highest degree of C=C conversion after 10 min. In the polymerization kinetics, PT4 had the highest maximum polymerization rate and reached earlier the transition between polymerization autoacceleration and autodeceleration. PT4 and SYS had significantly higher flexural strength and modulus than ST2 and PVP for most storage times. Results for Poisson ratio varied between materials. Longer storage periods were generally associated with higher frequency of catastrophic failures in the flexural tests. In conclusion, the performance of bis-acryl composite resins varied largely among materials.An Evaluation of the Light Output from 22 Contemporary Light Curing Units10.1590/0103-64402016014662024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZSoares, Carlos JoséRodrigues, Monise de PaulaOliveira, Laís Rani SalesBraga, Stella Sueli LourençoBarcelos, Luciana MendesSilva, Gisele Rodrigues daGiannini, MarceloPrice, Richard Bengt
<em>Soares, Carlos José</em>;
<em>Rodrigues, Monise De Paula</em>;
<em>Oliveira, Laís Rani Sales</em>;
<em>Braga, Stella Sueli Lourenço</em>;
<em>Barcelos, Luciana Mendes</em>;
<em>Silva, Gisele Rodrigues Da</em>;
<em>Giannini, Marcelo</em>;
<em>Price, Richard Bengt</em>;
<br/><br/>
Abstract This study measured the radiant power (mW), irradiance (mW/cm2) and emission spectra (mW/cm2/nm) of 22 new, or almost new, light curing units (LCUs): - Alt Lux II, BioLux Standard, Bluephase G2, Curing Light XL 3000, Demetron LC, DX Turbo LED 1200, EC450, EC500, Emitter C, Emitter D, KON-LUX, LED 3M ESPE, Led Lux II, Optilight Color, Optilight Max, Optilux 501, Poly Wireless, Radii cal, Radii plus, TL-01, VALO Cordless. These LCUs were either monowave or multiple peak light emitting diode (LED) units or quartz-tungsten-halogen LCUs used in anterior and posterior teeth. The radiant power emitted by the LCUs was measured by a laboratory grade laser power meter. The tip area (cm²) of the LCUs was measured and used to calculate the irradiance from the measured radiant power source. The MARC-Patient Simulator (MARC-PS) with a laboratory grade spectrometer (USB4000, Ocean Optics) was used to measure the irradiance and emission spectrum from each LCU three times at the sensor located on the facial of the maxillary central incisors and then separately at the occlusal of a maxillary second molar. The minimum acceptable irradiance level was set as 500 mW/cm2. Irradiance data was analyzed using two-way ANOVA and the radiant power data was analyzed by one-way ANOVA followed by Tukey test (a=0.05). In general, the irradiance was reduced at the molar tooth for most LCUs. Only the Valo, Bluephase G2 and Radii Plus delivered an irradiance similar to the anterior and posterior sensors greater than 500 mW/cm2. KON-LUX, Altlux II, Biolux Standard, TL-01, Optilux 501, DX Turbo LED 1200 LCUs delivered lower irradiance values than the recommended one used in molar region, KON-LUX and Altlux II LCUs used at the maxillary incisors. Bluephase G2 and Optilight Max delivered the highest radiant power and KON-LUX, Altlux II and Biolux Standard delivered the lowest power. The emission spectrum from the various monowave LED LCUs varied greatly. The multi-peak LCUs delivered similar emission spectra to both sensors.Antimicrobial Activity and Biocompatibility of the <i>Psidium cattleianum</i> Extracts for Endodontic Purposes10.1590/0103-64402016014092024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZMassunari, LoianeNovais, Renata ZoccalOliveira, Márcio TeixeiraValentim, DiegoDezan, EloiDuque, Cristiane
<em>Massunari, Loiane</em>;
<em>Novais, Renata Zoccal</em>;
<em>Oliveira, Márcio Teixeira</em>;
<em>Valentim, Diego</em>;
<em>Dezan, Eloi</em>;
<em>Duque, Cristiane</em>;
<br/><br/>
Abstract Psidium cattleianum (PC) has been displaying inhibitory effect against a variety of microorganisms, but this effect has not yet been tested against endodontic pathogens. The aim of this study was to evaluate the antimicrobial activity and biocompatibility of the aqueous (PCAE) and hydroethanolic (PCHE) extracts from Psidium cattleianum (PC) leaves. Minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) were determined using the microdilution broth method in order to analyze the antimicrobial effect against Enterococcus faecalis, Pseudomonas aeruginosa, Actinomyces israelii and Candida albicans in planktonic conditions. Biofilm assays were conducted only with the extracts that were able to determine the MLC for microorganisms in planktonic conditions. Immediate and late tissue reactions against PC extracts were evaluated using edemogenic test and histological analysis of subcutaneous implants in Wistar rats. The results showed that the MIC and MLC values ranged between 0.25 and 4 mg/mL. The MLC obtained for PCHE inhibited 100% growth of all the tested strains, except for C. albicans. PCAE had the same effect for E. faecalis and P. aeruginosa. Both PC extracts were able to eliminate E. faecalis biofilms and only the PCHE eliminated P. aeruginosa biofilms. The positive controls inhibited the growth of all tested strains in MIC and MLC essays, but no CHX tested concentrations were able to eliminate A. israelii biofilm. PCAE caused a discrete increase in the edema over time, while PCHE caused a higher initial edema, which decreased progressively. Both PCAE and PCHE extracts were biocompatible, but PCHE showed better results with slight levels of inflammation at 28 days. In conclusion, PCHE was biocompatible and presented better antimicrobial effect against important pathogens associated with persistent endodontic infectionsInfluence of Root Canal Filling Techniques on Sealer Penetration and Bond Strength to Dentin10.1590/0103-64402016024602024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZMacedo, Luciana Martins Domingues deSilva-Sousa, YaraSilva, Silvio Rocha Corrêa daBaratto, Samantha Schaffer PugsleyBaratto-Filho, FlaresAbi, Fuad Jacob
<em>Macedo, Luciana Martins Domingues De</em>;
<em>Silva-Sousa, Yara</em>;
<em>Silva, Silvio Rocha Corrêa Da</em>;
<em>Baratto, Samantha Schaffer Pugsley</em>;
<em>Baratto-Filho, Flares</em>;
<em>Abi, Fuad Jacob</em>;
<br/><br/>
Abstract The influence of root canal filling techniques on the penetration of AH Plus into dentinal tubules was evaluated using confocal laser scanning microscopy (CLSM) and the impact on the bond strength of the filling material on the root dentin was evaluated using the push-out test. Roots of the maxillary central incisors (n=40) were prepared with ProTaper Universal and assigned to four groups: lateral condensation, vertical compaction, modified Tagger hybrid and single cone. After root canal filling with gutta-percha and AH Plus, along with the addition of 0.01% fluorescein, the roots were cut into 2-mm slices. CLSM was performed on the first slice of each root third, followed by the push-out test. The second slice was analyzed under SEM. ANOVA demonstrated a statistically significant difference between the root canal filling techniques and the root thirds (p<0.05). For percent penetration, Tukey’s test showed that the vertical compaction (91.77±13.51), lateral condensation (89.18±10.91) and modified Tagger hybrid (87.53±15.42) presented the highest values, which significantly differed (p<0.05) from those of the single cone (78.19±22.46). For bond strength (MPa), lateral condensation (3.42±0.54) and vertical compaction (3.47±0.53) had the highest values (p<0.05), while the modified Tagger hybrid (2.19±0.41) and single cone (0.54±0.21) had the lowest. In conclusion, the lateral condensation, vertical compaction and Modified Tagger’s hybrid techniques resulted in greater penetration and bond strength to the intra-radicular dentin.Surgeons’ Performance Determining the Amount of Graft Material for Sinus Floor Augmentation Using Tomography10.1590/0103-64402016014422024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZCruz, Adriana DiboPeixoto, Guilherme AlvaresAguiar, Marcelo FreitasCamargo, Gabriela Alessandra Cruz GalhardoHoms, Nicolas
<em>Cruz, Adriana Dibo</em>;
<em>Peixoto, Guilherme Alvares</em>;
<em>Aguiar, Marcelo Freitas</em>;
<em>Camargo, Gabriela Alessandra Cruz Galhardo</em>;
<em>Homs, Nicolas</em>;
<br/><br/>
Abstract This study aimed to assess the performance of surgeons in determining the amount of graft material required for maxillary sinus floor augmentation in a preoperative analysis using cone-beam computed tomography images. A convenience sample of 10 retrospective CBCT exams (i-CAT®) was selected. Scans of the posterior maxilla area with an absence of at least one tooth and residual alveolar bone with an up to 5 mm height were used. Templates (n=20) contained images of representative cross-sections in multiplanar view. Ten expert surgeons voluntarily participated as appraisers of the templates for grafting surgical planning of a 10 mm long implant. Appraisers could choose a better amount of graft material using scores: 0) when considered grafting unnecessary, 1) for 0.25 g in graft material, 2) for 0.50 g, 3) for 1.00 g and 4) for 1.50 g or more. Reliability of the response pattern was analyzed using Cronbach’s a. Wilcoxon and Mann-Whitney tests were performed to compare scores. Regression analysis was performed to evaluate whether the volume of sinuses (mm3) influenced the choose of scores. In the reliability analysis, all values were low and the score distribution was independent of the volume of the maxillary sinuses (p>0.05), which did not influence choosing the amount of graft material. Surgeons were unreliable to determine the best amount of graft material for the maxillary sinus floor augmentation using only CBCT images. Surgeons require auxiliary diagnostic tools to measure the volume associated to CBCT exams in order to perform better.Are Maternal Factors Predictors for Early Childhood Caries? Results from a Cohort in Southern Brazil10.1590/0103-64402016010472024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZPinto, Gabriela dos SantosAzevedo, Marina SousaGoettems, Marília LeãoCorrea, Marcos BrittoPinheiro, Ricardo TavaresDemarco, Flávio Fernando
<em>Pinto, Gabriela Dos Santos</em>;
<em>Azevedo, Marina Sousa</em>;
<em>Goettems, Marília Leão</em>;
<em>Correa, Marcos Britto</em>;
<em>Pinheiro, Ricardo Tavares</em>;
<em>Demarco, Flávio Fernando</em>;
<br/><br/>
Abstract This study aimed to determine the influence of maternal factors on the early development of dental caries in Brazilian preschoolers. This cross-sectional study was nested in a cohort of adolescent mothers. The current wave was performed when the children were aged 24 to 42 months. The questionnaire-based survey targeted adolescent mothers and included demographic and socioeconomic variables as well as the maternal education level. In addition, clinical examinations were performed on the mothers and their children. Mothers were assessed for decayed, missing and filled teeth in the permanent dentition (DMFT index) and gingival assessment; their children were assessed for decayed, missing and filled teeth in the deciduous dentition (dmft index). Poisson regression with robust variance was used to estimate the prevalence ratio, risk ratio and 95% confidence intervals. This data was also used to identify the maternal risk factors associated with the outcomes (prevalence and severity of childhood caries). A total 538 mother-child dyads were evaluated; the prevalence of early childhood caries was 15.1% and maternal caries was 74.4%. After the adjustment, the children that exhibited a greater incidence of dental caries were from mothers of low socioeconomic status, or from those presenting decayed teeth and higher rates of gingival bleeding. The results of this study suggest that the oral health of mothers is a potentially important risk factor for the development of early childhood dental caries. Public health planners should consider this information when planning interventions in order to prevent the occurrence of early dental caries.Clinical and Individual Variables in Children’s Dental Fear: A School-Based Investigation10.1590/0103-64402016012652024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZSilveira, Ethieli Rodrigues daGoettems, Marília LeãoDemarco, Flávio FernandoAzevedo, Marina Sousa
<em>Silveira, Ethieli Rodrigues Da</em>;
<em>Goettems, Marília Leão</em>;
<em>Demarco, Flávio Fernando</em>;
<em>Azevedo, Marina Sousa</em>;
<br/><br/>
Abstract This cross-sectional study evaluated the prevalence of dental fear and associated factors in schoolchildren aged 8 to 12 years old, in Pelotas, southern Brazil. Schoolchildren enrolled in 20 public and private schools were selected using a multi-stage sample design. Sociodemographic characteristics, children’s dental visit and oral hygiene habits were assessed by questionnaires. The Dental Anxiety Question was used to measure dental fear prevalence. Children’s clinical examination evaluated presence of dental caries (DMFT/dmft index) and gingival bleeding. Data were analyzed using Poisson regression with robust variance (prevalence ratio; 95% confidence interval). One thousand two hundred and two children were included. Dental fear prevalence was 24.6%. After the adjustment, girls [PR=1.71 (CI 95%: 1.31-2.22)], children from poorer families [PR=1.96 (CI 95%: 1.36-2.83)], those who had decayed teeth (D/d index>0)[PR=1.32 (CI 95%: 1.01-1.72), and who had never been at the dentist [PR=1.85 (CI 95%: 1.42-2.41) remained significantly associated with dental fear. The prevalence of dental fear indicates that it is a common problem among schoolchildren. Early dental care and dental caries prevention are important factors to prevent dental fear.Oral Nodular Lesions in Patients with Sjögren’s Syndrome: Unusual Oral Implications of a Systemic Disorder10.1590/0103-64402016010132024-03-19T20:31:31.592000Z2020-08-09T06:48:16.145000ZPinheiro, Juliana BarchelliTirapelli, CamilaSilva, Claudia Helena Lovato daKomesu, Marilena ChinaliPetean, Flávio CalilLouzada, PauloLeón, Jorge EsquicheMotta, Ana Carolina Fragoso
<em>Pinheiro, Juliana Barchelli</em>;
<em>Tirapelli, Camila</em>;
<em>Silva, Claudia Helena Lovato Da</em>;
<em>Komesu, Marilena Chinali</em>;
<em>Petean, Flávio Calil</em>;
<em>Louzada, Paulo</em>;
<em>León, Jorge Esquiche</em>;
<em>Motta, Ana Carolina Fragoso</em>;
<br/><br/>
Abstract Sjögren’s syndrome (SS) is a systemic chronic autoimmune disorder affecting the lacrimal and salivary glands. SS may manifest as primary SS (pSS) or secondary SS (sSS), the latter occurring in the context of another autoimmune disorder. In both cases, the dry eyes and mouth affect the patient’s quality of life. Late complications may include blindness, dental tissue destruction, oral candidiasis and lymphoma. This paper reports two cases of SS, each of them presenting unusual oral nodular lesion diagnosed as relapsed MALT lymphoma and mucocele. The importance of the diagnosis, treatment and management of the oral lesions by a dentist during the care of SS patients is emphasized, as the oral manifestations of SS may compromise the patient’s quality of life.