Figure 8 - Double staining of COS-7 cells transfected with cDNA encoding the NT-1 receptor with fluo-NT (A) and acridine orange (B). Fluo-NT labeling was carried out for 30 min at 37oC. Cells were then rinsed and further incubated for 1 min with acridine orange. Note that both labels are co-localized within the same intracellular organelles, indicating that fluo-NT is internalized within acidic compartments. n, Nucleus. Scale bar: 10 Ám.