Figure 9 - Dual localization of internalized fluo-NT and immunoreactive NT-1 receptor in COS-7 cells transfected with cDNA encoding the NT-1 receptor tagged with an immunogenic epitope (VSV). Cells were incubated for 5 min with 20 nM fluo-NT, fixed with paraformaldehyde and immunolabeled for the tagged NT-1 receptor. Note that at this short time point there is complete overlap in the distribution of ligand (A) and receptor (B) confirming that internalization implicates receptor-ligand complexes. Scale bar: 5 Ám.