Figure 1 - Chromatography of the crude albumin fraction from O. ficus-indica seeds on a Sephacryl S-100-HR column. Proteins (60 mg in 4.0 ml of running buffer) were eluted with 50 mM ammonium bicarbonate at a flow rate of 30 ml/h and samples of 4.2 ml were collected. The fractions indicated by the horizontal bar were pooled and subjected to the next purification step.