Figure 2 - A, Chromatography of a fraction enriched with the 6.5-kDa protein on a Superdex Peptide HR 10/30 column. Proteins (1.0 mg in running buffer) were eluted with 50 mM ammonium bicarbonate at a flow rate of 0.5 ml/min and samples of 1.0 ml were collected. The fractions indicated by the horizontal bar were pooled and subjected to the next purification step. B, RP-HPLC of the fraction enriched with the 6.5-kDa protein on a -Bondapak C-18 column. Proteins (200 g) were eluted with a linear gradient of 0-60% acetonitrile in 0.1% trifluoracetic acid run for 60 min.