Figure 1 - Distribution of female rats according to the number of oocytes on the day of estrus. Each symbol represents an animal. Intact control rats were kept undisturbed during the neonatal period; handled animals were gently manipulated for 1 min daily from the 1st to the 10th day after delivery. After collection of vaginal smears for 29 days, the animals were anesthetized at 9:00 a.m. on the day of estrus, when ovaries were removed and the number of oocytes was counted.