Figure 3. HPLC profile showing the consumption of angiotensinogen (ANGTS) and generation of angiotensin I (AI). Angiotensinogen was incubated with renin from mesangial cells for 4, 8, 12 and 24 h (panels A, B, C and D, respectively). The retention time was 19.59 min for AI and 20.23 min for ANGTS.