Abnormal subcellular distribution of GLUT4 protein in obese and insulin-treated diabetic female dogs. A.M. Vargas, R.P.A. Barros, R.A. Zampieri, M.M. Okamoto, P. de Carvalho Papa and U.F. Machado. Brazilian Journal of Medical and Biological Research, 37 (7): 1095, 2004.
Figure 1. GLUT4 protein in control, obese and insulin-treated diabetic female dogs. The GLUT4 protein was assessed in the plasma membrane (PM) and microsome (M) subcellular fractions of white adipose tissue of control (open columns), obese (filled columns) and insulin-treated diabetic (hatched columns) female dogs. Equal amounts of total protein were immunoblotted to GLUT4. A, Top, representative autoradiograms of GLUT4 with the molecular weight of the blots; Bottom, results reported as arbitrary units (AU) per µg of total protein electrophoresed. In B, the results for the PM and M fractions are reported as AU per g tissue. In C, the sum of PM and M GLUT4 per g tissue was considered to be the total cellular GLUT4 content (100%). The percent of the total GLUT4 present in PM at the time of tissue removal is shown in C. D, The percent cellular GLUT4 in the PM of control and obese animals was divided by their insulinemia (µU/ml) at the time of tissue removal. Results are reported as the mean ± SEM for 4 animals analyzed in 4 different experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control value; #P < 0.05, ##P < 0.01, ###P < 0.001 vs obese value (ANOVA followed by the Student-Newman-Keuls post hoc test).