Normal skin and hypertrophic scar fibroblasts differentially regulate collagen and fibronectin expression as well as mitochondrial membrane potential in response to basic fibroblast growth factor. Rui Song, Hui-Ning Bian, Wen Lai, Hua-De Chen and Ke-Seng Zhao.
Figure 3. Effect of basic fibroblast growth factor (bFGF) on type I/III collagen and fibronectin production by normal and hypertrophic scar (HS) fibroblasts. The cells were grown and treated with bFGF at concentrations of 0.1 to 1000 ng/mL for 72 h and subjected to ELISA. bFGF decreased type I collagen production by HS fibroblasts and increased fibronectin production by normal fibroblasts. Data are reported as means ± SEM. The experiments were carried out in triplicate and repeated at least once. **P < 0.01 vs untreated fibroblasts; #P < 0.05, ##P < 0.01 vs normal skin fibroblasts (paired t-test and one-way randomized ANOVA).