Normal skin and hypertrophic scar fibroblasts differentially regulate collagen and fibronectin expression as well as mitochondrial membrane potential in response to basic fibroblast growth factor. Rui Song, Hui-Ning Bian, Wen Lai, Hua-De Chen and Ke-Seng Zhao.
Figure 4. Effect of basic fibroblast growth factor (bFGF) on the expression of type I/III collagen and fibronectin mRNA in normal and hypertrophic scar (HS) fibroblasts. The cells were grown and treated with bFGF at concentrations of 0.1 to 1000 ng/mL for 72 h and then subjected to RT-PCR analysis of type I (A), type III collagen (B), and fibronectin (C) expression. bFGF down-regulated type I collagen mRNA in HS fibroblasts and up-regulated fibronectin mRNA in normal fibroblasts. Data were normalized to a β-actin loading control. The bottom graphs showed the quantitative RT-PCR data. Data are reported as means ± SEM. *P < 0.05, **P < 0.01 vs untreated fibroblasts; #P < 0.05, ##P < 0.01 vs normal skin fibroblasts (paired t-test and one-way randomized ANOVA).