Normal skin and hypertrophic scar fibroblasts differentially regulate collagen and fibronectin expression as well as mitochondrial membrane potential in response to basic fibroblast growth factor. Rui Song, Hui-Ning Bian, Wen Lai, Hua-De Chen and Ke-Seng Zhao.
Figure 5. Flow cytometry analysis of the mitochondrial membrane potential in normal and hypertrophic scar (HS) fibroblasts. Cells were grown and treated with basic fibroblast growth factor (bFGF) at concentrations of 0.1 to 1000 ng/mL for 72 h and then subjected to JC-1 staining and flow cytometry. The relative mitochondrial membrane potential was expressed as the ratio of JC-1 red fluorescence (upper quadrant) to green fluorescence (lower right quadrant) in the normal (A, top) and HS fibroblasts (A, bottom). bFGF depolarized HS fibroblasts mitochondria, with no effect on mitochondrial membrane potential in normal fibroblasts. The quantitative data (B) are reported as means ± SEM. **P < 0.01 vs untreated fibroblasts (paired t-test and one-way randomized ANOVA).