This study treats the distribution and association of the species of a bats in culverts along the BR-174 road 25-75 km north of Manaus, AM. The physical characteristics of the culverts varied, and two factors, the presence of stagnant watch, and large free internal space, were significantly correlated with large concentrations of Carollia perspicillata. This paper records the first interspecific associations between Trachops cirrhosus and Macrophyllum, and between T. cirrhosus and Anoura caudifer.