New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection

Novos registros de Leptospira spp. em marsupiais e um roedor silvestres na Amazônia Oriental por detecção por PCR

Katarine de SOUZA ROCHA Gleiciane SCHUPP DE SENA MESQUITA Maeli Fernanda SILVA FERREIRA Flávia de Nazaré LEITE BARROS Renata Cecília Soares de Lima MACEDO Elane de ARAÚJO SARAIVA Ana Cristina MENDES-OLIVEIRA Valíria DUARTE CERQUEIRA Alessandra SCOFIELD Gustavo GOES CAVALCANTE Isis ABEL Carla Cristina GUIMARÃES DE MORAES About the authors

ABSTRACT

We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.

KEYWORDS:
Didelphimorphia; Rodentia; live traps; kidney; liver; 16S rRNA

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