Bovine meat and bone meal is an economically viable alternative in quail feeding in the initial phase

An Acad Bras Cienc aabc Anais da Academia Brasileira de Ciências An. Acad. Bras. Ciênc. 0001-3765 1678-2690 Academia Brasileira de Ciências A produção de ovos de codorna tem experimentado um aumento acentuado na última década. A nutrição é o principal fator que afeta o potencial produtivo na indústria de aves, assim como o manejo nutricional adequado é necessário para garantir a manutenção das condições físicas ideais, crescimento e produção de produtos de alta qualidade. A farinha de carne e ossos (FCO) tem sido frequentemente utilizada na indústria avícola como uma fonte alternativa e de baixo custo de proteína em substituição parcial do farelo de milho e soja. No entanto, até esta data, não houve estudos que tinham investigado o efeito da dieta FCO sobre o desempenho de codornas ou sobre os custos de produção na fase inicial de criação. Isto é particularmente importante tendo em vista que esta fase é caracterizada por grandes investimentos por parte dos produtores, sem retorno econômico imediato. Neste estudo, nós investigamos se a substituição parcial do farelo de soja (FS) por farinha de carne e ossos (FCO) na dieta de codornas japonesas durante a fase inicial de criação é uma alternativa viável que venha a manter ou melhorar o seu desempenho produtivo e econômico. Nossos resultados mostram que a inclusão da FCO na dieta de codornas reduz os custos de alimentação por até 6% sem prejudicar o desempenho produtivo. INTRODUCTION Quail egg production has experienced a steep rise in the last decade. Fast growth rates, early sexual maturity, high longevity and lower production costs compared to those of laying hens are all contributing factors to this trend (Pastore et al. 2012). Nutrition is the main factor affecting productive potential in the poultry industry, as appropriate nutritional management is necessary to ensure the maintenance of optimal physical conditions, growth and the production of high quality products (Costa et al. 2010). However, because the price of feed is also the main determinant of production costs (approximately 70% of overall production costs), the investigation of diets promoting high product quality at reduced cost has been a constant target of research (Embrapa Meio-Norte 2007). Meat and bone meal (MBM) has often been used in the poultry industry as an alternative and cost-effective source of protein in partial replacement of corn and soybean meal, the main raw materials used in bird diets. MBM (a byproduct of bovine meat production) is not only cheaper, but also a source of crude protein content (ranging from 35 to 55%) as well as of calcium and high availability phosphorous. This is particularly important considering that phosphorous is the third limiting factor relative to the cost of feed formulations (Campestrini 2005). MBM has also been shown to improve odor, texture and palatability of diets when used within appropriate nutritional thresholds (Lessire et al. 1985, Martosiswoyo and Jensen 1988, Jensen 1991, Dale 1997). Quail use corn and soybean meal energy similarly to broilers and laying hens. However, the nutritional requirements of quail are different (Silva et al. 2012), as their protein requirement is higher than that of broilers, resulting in a higher feeding cost per unit of product (Silva et al. 2012). Thus, partial replacement of corn and soybean meal by MBM in the diet of quail could be a viable mean of ensuring an appropriate diet at reduced cost. To our knowledge, however, there have been no studies to date that have investigated the effect of dietary MBM on the performance of quail or on the costs of production in the starter phase. This is particularly important considering that this phase is characterized by large investments by producers, without immediate economic return. Ensuring appropriate development and growth during the starter phase is also critical for optimal productive performance in the long-term. In this study we investigate whether partial replacement of soybean meal (SBM) by meat and bone meal (MBM) in the diet of Japanese quail during the starter phase is a viable alternative that would maintain or improve their productive and economic performance. Our results show that the inclusion of MBM in the diet of quail reduces feeding costs by up to 6% without impairing productive performance. MATERIALS AND METHODS The experiment was conducted in a poultry shed of the Agência Paulista de Tecnologia em Agronegó cios, in Brotas, São Paulo, Brazil, in order to evaluate the effect of different levels of partial replacement of soybean meal (SBM) by meat and bone meal (MBM) in the diet of Japanese quail during the starter phase (1-35 days old). Seven hundred and twenty, 1-day-old quail were housed in a shed measuring 3.0 x 12.0 m, containing 24 boxes suitable for rearing at this stage. The 1.0 m2 boxes were equipped with feeders and waterers (pressure cup type). The quail, housed 30 per box, were heated with infrared lamps until 21 days of age in order to provide the proper temperature. The quail were subjected to identical conditions of management and feeding, with water and feed ad libitum. At seven days of age, the birds were vaccinated against Newcastle disease and at 21 days against fowlpox. The lighting program used was one of 23 h of light in the first week and then natural light until 35 days of age. All procedures were carried out according to the international practices for animal use and care under the control of an internal committee of the Institute of Animal Science, São Paulo, Brazil. The experimental design was completely randomized, with six experimental treatments, four replications and 30 animals per replication (Table I). TABLE I - Distribution of the experimental diets, using six levels of addition of bovine meat and bone meal (MBM) in the diets in place of soybean meal. Experimental Treatments T1 (control) Diet on the basis of corn, soybean meal and wheat + 0% MBM T2 Diet on the basis of corn, soybean meal and wheat + 1% MBM T3 Diet on the basis of corn, soybean meal and wheat + 2% MBM T4 Diet on the basis of corn, soybean meal and wheat + 3% MBM T5 Diet on the basis of corn, soybean meal and wheat + 4% MBM T6 Diet on the basis of corn, soybean meal and wheat + 5% MBM The experimental diets (Table II) were all isonutritive and formulated on the basis of corn and soybean meal as the raw materials, with levels of protein, calcium, phosphorus, metabolizable energy and amino acids established by Rostagno and Albino (2005) in order to meet the nutrient requirements of quail. TABLE II - Percent composition of feedstuffs and calculated nutrient levels of the experimental diets for Japanese quail during the starter period. Feedstuffs (%) Experimental Diets T1 T2 T3 T4 T5 T6 Ground corn 51.93 52.67 53.41 54.15 54.89 55.63 Soybean meal (45.5%) 42.07 41.06 40.04 39.03 38.02 37.00 Meat and bone meal 0.00 1.00 2.00 3.00 4.00 5.00 Soybean oil 2.28 2.05 1.82 1.59 1.36 1.13 Calcitic limestone (38.4% Ca) 0.866 0.738 0.610 0.481 0.353 0.225 Dicalcium phosphate 1.864 1.504 1.145 0.785 0.425 0.066 Common salt 0.398 0.387 0.376 0.366 0.355 0.344 Vitamin and mineral supplement1 0.60 0.60 0.60 0.60 0.60 0.60 Total 100 100 100 100 100 100 Nutrient Levels Calculated Metabolizable energy (kcal/kg) 2930 2930 2930 2930 2930 2930 Crude protein (%) 23.50 23.50 23.50 23.50 23.50 23.50 Crude fiber (%) 3.828 3.790 3.753 3.715 3.678 3.640 Calcium (%) 0.980 0.980 0.980 0.980 0.980 0.980 Available phosphorus (%) 0.480 0.480 0.480 0.480 0.480 0.480 Methionine (%) 0.534 0.533 0.533 0.532 0.532 0.531 Methionine + Cystine (%) 0.906 0.903 0.901 0.898 0.895 0.892 Lysine (%) 1.323 1.315 1.306 1.298 1.289 1.280 1Composition per kg of feed: Pantothenic acid 5,000.000 mg; Choline 43,400.000 mg; Niacin 6,680.000 mg; Vit. A 1,660,000.00 UI; Vit. B1 500,000 mg; Vit. B12 1,680.000 mg; Vit B2 1,000.000 mg; Vit. B6 666.700 mg; Vit. D3 208.400000 UI; Vit. E 3.360.000 mg; Vit. K3 500.000 mg; Cu 1,333.300 mg; Fe.8,33300 mg; I. 200.000 mg; Mn.11,666.600 mg; Zn. 8,333.300 mg; Se 33.330mg, Growth promoter 1,166.670 mg, Antioxidant 16,680.000 mg, q.s.p. carrier 1,000.000 g. The MBM was analyzed bromatologically and contained 93.75% dry matter (DM), 45.75% crude protein (CP), 8.43% ether extract (EE), and 48.17% mineral matter (MM). MBM was added to the experimental diets according to the treatment used. The production characteristics evaluated weekly, were weight gain (g), feed intake (g), feed conversion (FC) and mortality. Statistical analysis by ANOVA and contrasts between treatment means by the Tukey test at 5% were carried out with aid of the computer package Sisvar as described by Ferreira (2011). Economic performance was evaluated by using the price per kilogram of feed of each treatment, multiplied by the average amount of feed consumed by the quail until 35 days of age and added to the price of the quail. The values ​​used were obtained by the site www.ovooline.com.br and www.iea.sp.gov.br of the Institute of Agricultural Economics (IEA) in September 2014. RESULTS AND DISCUSSION Minimum (21.1 °C) and maximum (27.8 °C) temperatures in the experimental shed were within the thermal comfort zone for Japanese quail in all treatments (Table III). There were, however, no significant differences (p > 0.05) among treatments for any of the performance variables (Table III). TABLE III - Performance of Japanese quail aged 1 to 35 days fed with diets with increasing proportion of meat and bone meal (MBM) in partial replacement of soybean meal. Performance (average) Treatments IBW1 (g) FBW2 (g) WG3 (g) FI4 (g) FC5 Mortality (%) (1 d) (35 d) (1 to 35 d) 0% MBM 7.02 132.77 122.54 426.73 3.43 00.40 1% MBM 7.28 128.91 119.18 411.84 3.39 00.30 2% MBM 7.18 131.52 122.87 427.44 3.47 00.50 3% MBM 7.14 126.70 118.86 405.19 3.40 00.20 4% MBM 7.08 128.27 116.80 416.53 3.45 00.90 5% MBM 7.13 126.47 118.18 415.12 3.50 00.20 Mean 7.14 129.11 119.74 417.14 3.44 00.40 CV (%) 3.57 002.24 002.99 004.39 4.43 79.25 1Initial body weight, 2final body weight, 3weight gain, 4feed intake, 5feed conversion. Our observation that the inclusion of MBM did not impair the performance of quail is consistent with a previous finding based on the use of this feed in partial replacement of corn and soybean meal in the diet of broilers (Bozkurt et al. 2004), where the authors observed no deleterious effect on either, performance or mortality. Similarly, Silva and Brandão (2011) have recently observed an increase in performance following the partial replacement of corn and soybean meal in the diet of quail during the starter phase. However, these authors used blood meal as the alternative feed source, as opposed to MBM. Our study is therefore the first to show that MBM can partially replace corn and soybean meal for quail during the starter phase without any impairment in performance - a finding still controversial in other bird production systems such as that of broilers (Faria Filho et al. 2002). We also analyzed differences in the cost of the experimental diets, as measured per kilogram of feed and per bird (Table IV). TABLE IV - Cost of diets (in US$) with increasing proportions of meat and bone meal (MBM) in replacement of soybean meal fed to Japanese quail aged 1 to 35 days. Individual quail prices were the same for all diets (US$ 0.192). Treatments (% MBM) 0 1 2 3 4 5 Cost of diets (US$/kg) 0.400 0.395 0.390 0.385 0.380 0.375 % of increase in cost relative to 5% MBM 6% 5% 4% 3% 1% 0% Diet cost/bird over the period (35 days) 0.169 0.161 0.165 0.157 0.157 0.157 Our results showed the existence of economic benefits in formulating feed for quail during the initial phase with MBM in partial substitution of soybean meal, compared to feed formulated only with soybean meal as the main protein source. The results emphasize the notion that a reduction in the cost of food is closely related to the use of dietary nutrients, in this case effectively replacing soybean meal and dicalcium phosphate, which are the most expensive ingredients in the formulation of diets. Incorporation of MBM in the quail diet at a level of 3% resulted in an average reduction of up to 6% in the cost of feed, allowing savings of up to US$0.00072/day/bird, or US$0.026/bird over the 35 days comprising the starter period. For production plants with 25,000 to 150,000 birds, this could represent a saving of approximately US$650 to US$3,900 during this period. It is possible to incorporate MBM as a repla cement for soybean meal in starter phase diets for quail at levels as high as 5% without loss in productive performance. Taking into account the price per bird and the individual cost of feed, this replacement represents savings of up to 6% of the diet cost during this phase. ACKNOWLEDGMENTS We would like to thanks Mr. Rocha from VICAMI, for donation of the quails and Kakimoto Farm for donation of feedstuffs and preparation of the diets used in this experiment. We are also grateful to Mr. Mizuma from Nutribastos, for donation of the vaccines and Mr. José Roberto Medina Garcia and Eduardo Piber Neto from BRNova who provided assistance with the laboratory analyses. Bozkurt M, Basmacioglu H and Ergul M. 2004. Effect of Dietary Concentration Meat and Bone Meal on Broiler Chickens Performance. 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