A New Species of Hershkovitzia (Diptera: Nycteribiidae) from Maranhão, Brazil

: Hershkovitzia Guimarães & D’Andretta, 1956 belongs to Nycteribiidae, composed of hematophagous species exclusively ectoparasites of bats. The new species was collected from the bat Thyroptera devivoi Gregorin, Gonçalves, Lim & Engstrom, 2006 (Chiroptera: Thyropteridae) from Barreirinhas in Maranhão State, Brazil. Herein, we proposed schematic drawings of the abdomen, legs and head. We also proposed an identifi cation key to species of Hershkovitzia .

H e rs h k o v i t z i a i s m o r p h o l o g i c a l l y characterized as follows: head laterally compressed, pigmented eyes formed by a single facet, thin pedipalps, and presence of thoracic ctenidium.The abdomen of females has seven dorsal sclerotined plates (syntergites 1+2 and fi ve tergites), seven ventral ones (sternite 1+2 and fi ve sternites), and one genital plate.Males present six dorsal plates (syntergite 1+2 and four tergites), fi ve ventrals (sternite 1+2 and three sternites), a genital plate and phallobase divided longitudinally in two (Guimarães & D'Andretta 1956).Guimarães & D'Andretta proposed Hershkovitzia in 1956 in a synopsis of American species of Nycteribiidae.The type species was designated by monotype (H.primitiva).Later, three more species were described: H. coeca, H. inaequalis and H. cabala.
In this work, a new species of the genus Hershkovitzia is described from the northeast coast of Brazil.Along with the species description, we also provide illustrations and an identification key.

MATERIALS AND METHODS
Adult flies were collected in Tabocas, B a r re i r i n h as m u n i c i pal i t y, M a ra n h ã o ( 2 º 5 8 ' 3 8 .5 7 " S / 4 3 º 1 0 ' 0 9 .9 2 " W a n d 2º59'56.92"S/43º07'55.22").The host bat specimens were captured in mist nets and the flies removed with tweezers.The bat flies were preserved in 70% ethanol.Morphological terminology follows Guimarães & D'Andretta (1956) and Graciolli (2010).The plate numbers were used in Arabic and the divisions of the legs in Roman, as proposed by Theodor (1967).
The proposed drawings serve as a likeness only for the purpose of species identification.The regions chosen for the drawings are important for species diagnosis: abdomen of female and male (ventral and dorsal), head (dorsal and lateral) and tibia II.The drawings were done freehand in graphite on graph paper with the assistance of a stereomicroscope.The finalization of the drawings was in made on vegetal paper and nanquim and later scanned with an HP Deskjet 2600.Subsequently, the drawings were vectored and edited, using Corel DRAW 2018.Drawings were saved in 1200 dpi quality.
Etymology: Specific epithet after Dr. Maria Aparecida Vulcano D'Andretta, for her contribution to the genus.
Diagnosis: Each eye with three setae, one seta located in the region between the eye and the others located before the eye.Gena with two setae; post-gena with two short setae.Three setae located anterior to meso-notopleural sutures.Female syntergites 1+2 and tergites 3 and  (2022) 94(1) e20191161 3 | 7 7 made up a whole plate; tergite 5 and 6 divided into two plates each.Sternite 1+2 consisting of a whole plate; sternites 3, 4, 5 and 6 divided into two plates each.Sternite 7 subtriangular with lateral and median portions with depressions, strongly pigmented and without setae.Male presents all tergites and sternites formed by a whole plate.
Descripton: Head.Dorsal view -eyes with one facet; one seta medial to and near hind margin of each eye, and two setae directly in front of each eye, more evident in male (Fig. 1a).Gena with three setae, two short and one long in the middle.Post-gena with two setae (Fig. 1b).Ventral view -palps on the distal and proximal distend, with 10 setae along palps length, and four longer setae at the distal margin.Thecae wider than long.
Thorax: Dorsal view -Meso-notopleural suture with three setae.Thoracic ctenidium with 10 spines.Thoracic spiracle piriform shape.Ventral view -sternal plate with setae throughout length.Angle between oblique suture greater than 90º.Coxa I as long as wide, flattened, triangular, and without setae on anterior part.Femur I longer than wide, flattened, rectangular, ring visible and with setae throughout length.Tibia I flattened, cylindrical, and with four ventrodistal rows of setae on apical portion.Basitarsus the same size as the next three tarsi.Coxa II short, triangular and with short setae.Femur II and III longer than wide, flattened, rectangular, with setae throughout length.Visible ring, with a row of setae on anterior margin.Tibia II longer than wide, cylindrical, with setae throughout length and distal posterior margin with three rows of setae on apical portion (Fig. 2).Coxa III as wide as long, triangular, with setae throughout length.Tibia III cylindrical, with five rows of setae on apical portion.
Female.Abdomen: Tergites and sternites with numerous setae throughout length.All plates with distal margin and row of setae of unequal length.Dorsal view -Syntergite 1+2, tergites 3 and 4 entire.Tergite 5 and 6 with two  plates each.Tergite 7 short and with four setae along the plate.Tergite 7 with two rectangular side lobes and 4-5 setae in each lateral prominence (Fig. 3a).Ventral view -Sternite 1+2 rectangular with round margin.Ctenidium with 35 spines.Sternites 3, 4 and 5 with two plates each.Sternites 6 with two subtriangular plates.Sternite 7 subtriangular, with posterior margin almost straight and depressions on lateral and median portion of plate (Fig. 3b).
Male.Abdomen: Tergites and sternites with numerous setae throughout length.Dorsal view -All plates with distal margin with row of setae of unequal length.Syntergite 1+2 long and triangular.Tergite 3 to 6 entire.Microtrichia on anal plate and setae only on posterior margin (Fig. 4a).Ventral view -Sternite 1+2, ctenidium with 50 spines, covers the entire back margin of the plate.Sternite 3 to 5 as whole plate.Sternite 5 with concavity on median posterior margin and with round lateral margins.Lateral margin with a set of setae more sclerotized than the other setae on lateral abdomen (Fig. 4b).-Each eye with two associated setae, both of which are located anterior to the eyes.Mesonotopleural suture with three associated setae.Tergite 6 divided.Post-gena with three short setae.Gena with three setae……………Hershkovitzia primitiva Guimarães & D'Andretta.

Identification Key to species of Hershkovitzia Guimarães & D'Andretta
4. -Each eye with four associated setae, all located anterior to the eyes.Meso-notopleural

DISCUSSION
As previously noted in the key, Hershkovitzia females can be divided into two groups.The first group is characterized by having a row of abdominal ctenidium covering the entire posterior margin of sternite 1+2, in addition to tibia II with two rows in the median portion.This group is composed of H. primitiva, H. coeca and H. cabala.Hershkovitzia coeca differs from the other two species by the absence of eyes.In addition to other characters highlighted in the key, H. cabala differs by having all tergites composed of one plate, and H. primitiva differs by having tergite 6 divided into two plates.
Hershkovitzia inaequalis and Hershkovitzia mariae sp.nov compose the second group.This group is characterized by a row of ctenidium in sternite 1 + 2 covering only one third of the posterior margin, apart from tibia II with three rows in the apical portion.H. inaequalis can be differentiated by having tergite 4 and sternites 3, 4, 5 and 6 divided into plates.On the other hand, tergite 4 of Hershkovitzia mariae sp.nov. is composed of a single plate.In H. inaequalis, the row of ctenidium has 45 thin spines, and in Hershkovitzia mariae sp.nov., we observe 35 spines, thick and dense.
Hershkovitzia inaequalis and Hershkovitzia mariae sp.nov.have characteristics that can be seen in males and females.The head in H. inaequalis features four setae in each eye, while Hershkovitzia mariae sp.nov.has three setae in each eye.In H. inaequalis, the setae are located anterior to the eye, while in Hershkovitzia mariae sp.nov., two setae are located anterior to the eye, and the other seta is located between the eyes.(Fig 1a).
Hershkovitzia males are similar to Basilia males (Guimarães & D'Andretta 1956).However, they have very subtle characteristics, making their characterization and differentiation difficult.The male of Hershkovitzia mariae sp.nov., as with the other species of the genus, has inconsistent features with respect to the abdomen.The head, thorax and legs are similar to those of the female.
Hershkovitzia mariae sp.nov. is known only to Barrerinhas, state of Maranhão.This region represents the northern limit of the Cerrado in Brazil (Santos et al. 2013).This species is the first registered to the genus Hershkovitzia for the Cerrado biome (tropical savannah), while the other species occur in the Amazon rainforest (Graciolli et al. 2007, Graciolli & Dick 2018).
GABRIELA HRYCYNA et al.NEW SPECIES OF Hershkovitzia FROM MARANHÃO, BRAZIL An Acad Bras Cienc