Tracheal regeneration with acellular human amniotic membrane and 15-deoxy-∆12,14prostaglandinj2 nanoparticles in a rabbit model

MIGGIOLARO, ANNA FLAVIA R.S.; TAKEJIMA, ALINE L.; FRANCISCO, JULIO CESAR; NORONHA, LÚCIA DE; TAKEJIMA, MILKA L.; FOLTZ, KATIA; ENCK, ALOYSIO; TANNOUS, LUANA; SIMEONI, ROSSANA B.; STRICKER, PRISCILA E.F.; CARVALHO, KATHERINE A.T.; GUARITA-SOUZA, LUIZ CESAR

doi:10.1590/0001-3765202320220288

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HEALTH SCIENCES • An. Acad. Bras. Ciênc. 95 (suppl 1) • 2023 • https://doi.org/10.1590/0001-3765202320220288 linkcopy

Tracheal regeneration with acellular human amniotic membrane and 15-deoxy-∆12,14prostaglandinj2 nanoparticles in a rabbit model

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The treatment of tracheal pathologies remains challenging.Nanotechnology allows adding substances to decellularized human amniotic membrane (DHAM), such as 15-Deoxy-∆12,14ProstaglandinJ2 nanoparticles (15D-PGJ2-NC).This study performed a tracheotomy in rabbits randomized into three groups.The tissue repair process was evaluated when treated with DHAM associated or not with 15D-PGJ2-NC.The average of the area in the control group was 54.76% smaller than DHAM group and 41.98% smaller than DHAM + 15D-PGJ2-NC group (p=0.004 for both).The DHAM + 15D-PGJ2-NC group had significantly more immature cartilage (p=0.015).DHAM impregnated with 15D-PGJ2-NC could provide support for the healing of the tracheal defect and may prevent reduction of its lumen.

Key words
15D-PGJ2; amniotic membrane; healing; tracheal regeneration; tracheotomy

Authorship

person ANNA FLAVIA R.S. MIGGIOLARO

acquisition · analysis · interpretation of data

schoolPontifical Catholic University of Paraná (PUC-PR), Experimental Laboratory of the Institute of Biology and Health Sciences, R. Imaculada Conceição, 1155, Prado Velho, 80215-901 Curitiba, PR, BrazilPontifical Catholic University of Paraná (PUC-PR)BrazilCuritiba, PR, BrazilPontifical Catholic University of Paraná (PUC-PR), Experimental Laboratory of the Institute of Biology and Health Sciences, R. Imaculada Conceição, 1155, Prado Velho, 80215-901 Curitiba, PR, Brazil

0000-0002-8497-400X

person ALINE L. TAKEJIMA

acquisition · analysis · interpretation of data · drafting · revising the manuscript · final approval of the version to be published

0000-0002-2608-0171

person JULIO CESAR FRANCISCO

acquisition · analysis · interpretation of data

0000-0003-1970-6399

person LÚCIA DE NORONHA

acquisition · analysis · interpretation of data

0000-0003-0310-7164

person MILKA L. TAKEJIMA

acquisition · analysis · interpretation of data · drafting · revising the manuscript · final approval of the version to be published

schoolMackenzie Evangelical University Hospital, Plastic Surgery and Burns Department, Al. Augusto Stellfeld, 1908, Bigorrilho, 80730-150 Curitiba, PR, BrazilMackenzie Evangelical University HospitalBrazilCuritiba, PR, BrazilMackenzie Evangelical University Hospital, Plastic Surgery and Burns Department, Al. Augusto Stellfeld, 1908, Bigorrilho, 80730-150 Curitiba, PR, Brazil

0000-0002-0266-4076

person KATIA FOLTZ

acquisition · analysis · interpretation of data

0000-0002-9188-7262

person ALOYSIO ENCK

acquisition · analysis · interpretation of data

0000-0003-0458-5707

person LUANA TANNOUS

acquisition · analysis · interpretation of data

0000-0003-3559-1712

person ROSSANA B. SIMEONI

acquisition · analysis · interpretation of data

0000-0002-9976-5809

person PRISCILA E.F. STRICKER

drafting · revising the manuscript · final approval of the version to be published

schoolInstitute Pelé Pequeno Príncipe, Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department Research, Av. Silva Jardim, 1632, Água Verde, 80250-060 Curitiba, PR, BrazilInstitute Pelé Pequeno PríncipeBrazilCuritiba, PR, BrazilInstitute Pelé Pequeno Príncipe, Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department Research, Av. Silva Jardim, 1632, Água Verde, 80250-060 Curitiba, PR, Brazil

0000-0002-2582-137X

person KATHERINE A.T. CARVALHO

drafting · revising the manuscript · final approval of the version to be published

0000-0003-4860-2855

person LUIZ CESAR GUARITA-SOUZA

drafting · revising the manuscript · final approval of the version to be published

0000-0003-2781-9705

Correspondence to: Aline Luri Takejima E-mail:
alinetakejima@yahoo.com.br

SCIMAGO INSTITUTIONS RANKINGS

Pontifical Catholic University of Paraná (PUC-PR), Experimental Laboratory of the Institute of Biology and Health Sciences, R. Imaculada Conceição, 1155, Prado Velho, 80215-901 Curitiba, PR, BrazilPontifical Catholic University of Paraná (PUC-PR)BrazilCuritiba, PR, BrazilPontifical Catholic University of Paraná (PUC-PR), Experimental Laboratory of the Institute of Biology and Health Sciences, R. Imaculada Conceição, 1155, Prado Velho, 80215-901 Curitiba, PR, Brazil

Mackenzie Evangelical University Hospital, Plastic Surgery and Burns Department, Al. Augusto Stellfeld, 1908, Bigorrilho, 80730-150 Curitiba, PR, BrazilMackenzie Evangelical University HospitalBrazilCuritiba, PR, BrazilMackenzie Evangelical University Hospital, Plastic Surgery and Burns Department, Al. Augusto Stellfeld, 1908, Bigorrilho, 80730-150 Curitiba, PR, Brazil

Institute Pelé Pequeno Príncipe, Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department Research, Av. Silva Jardim, 1632, Água Verde, 80250-060 Curitiba, PR, BrazilInstitute Pelé Pequeno PríncipeBrazilCuritiba, PR, BrazilInstitute Pelé Pequeno Príncipe, Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department Research, Av. Silva Jardim, 1632, Água Verde, 80250-060 Curitiba, PR, Brazil

Figures | Tables

Figures (3)
Tables (3)

Thumbnail

Figure 1 The tracheal defect. a. Image of the surgical site, in which the arrowhead shows the tracheal defect by the tracheotomy in an animal in the control group. b. Image of the surgical site showing the tracheal defect covered by the amniotic membrane (arrowhead), in an animal in the DHAM group. c. Image of the trachea after resection of an animal in the DHAM + 15D-PGJ2-NC group. The arrowhead points to the area of the previous tracheotomy.

Thumbnail

Figure 2 Comparison of the cartilaginous tissue (aggrecan) between groups. AM, Amniotic Membrane; PGJ2, ProstaglandinJ2.

Thumbnail

Figure 3 Photographs of the performed stains and immunohistochemistry. a. Hematoxylin-Eosin (HE) staining of the lesion area (arrowhead) in a control group. b. HE Stain of the lesion in the DHAM group in which the arrowhead points to the remaining tissue of the amniotic membrane. c. HE Stain of the lesion in the DHAM + 15D-PGJ2-NC group in which the arrowhead points to the remaining tissue from the amniotic membrane added to 15D-PGJ2-NC, and the double arrowhead to the inflammatory infiltrate. d. Image of Sirius Red stain, at the region of the lesion, in the control group. The red images represent type I collagen (arrowhead), and the green images show type III collagen (double arrowhead). e. Collagen type III (arrowhead) and type I (double arrowhead) of the DHAM group. f. Collagen type III (arrowhead) and type I (double arrowhead) of the DHAM + 15D-PGJ2-NC group. g. Anti-aggrecan immunohistochemistry (40x magnification) in a control group animal. The arrowhead points to the aggrecan (ocher color) and the double arrowhead points to mature cartilage. h. Anti-aggrecan immunohistochemistry (10x magnification) in an DHAM group animal. The arrowhead points to the injury site. i. Anti-aggrecan immunohistochemistry in DHAM + 15D-PGJ2-NC group animal. The arrowhead points to the injury site.

Thumbnail

Table I Comparison of the area at the site of the tracheal defect between groups, two by two.

Thumbnail

Table II Comparison of averages indices of histological findings and characterization of the inflammatory profile.

Thumbnail

Table III Comparison of the cartilaginous tissue (aggrecan) between groups, two by two.

imageFigure 1 The tracheal defect. a. Image of the surgical site, in which the arrowhead shows the tracheal defect by the tracheotomy in an animal in the control group. b. Image of the surgical site showing the tracheal defect covered by the amniotic membrane (arrowhead), in an animal in the DHAM group. c. Image of the trachea after resection of an animal in the DHAM + 15D-PGJ2-NC group. The arrowhead points to the area of the previous tracheotomy. open_in_new

imageFigure 2 Comparison of the cartilaginous tissue (aggrecan) between groups. AM, Amniotic Membrane; PGJ2, ProstaglandinJ2. open_in_new

imageFigure 3 Photographs of the performed stains and immunohistochemistry. a. Hematoxylin-Eosin (HE) staining of the lesion area (arrowhead) in a control group. b. HE Stain of the lesion in the DHAM group in which the arrowhead points to the remaining tissue of the amniotic membrane. c. HE Stain of the lesion in the DHAM + 15D-PGJ2-NC group in which the arrowhead points to the remaining tissue from the amniotic membrane added to 15D-PGJ2-NC, and the double arrowhead to the inflammatory infiltrate. d. Image of Sirius Red stain, at the region of the lesion, in the control group. The red images represent type I collagen (arrowhead), and the green images show type III collagen (double arrowhead). e. Collagen type III (arrowhead) and type I (double arrowhead) of the DHAM group. f. Collagen type III (arrowhead) and type I (double arrowhead) of the DHAM + 15D-PGJ2-NC group. g. Anti-aggrecan immunohistochemistry (40x magnification) in a control group animal. The arrowhead points to the aggrecan (ocher color) and the double arrowhead points to mature cartilage. h. Anti-aggrecan immunohistochemistry (10x magnification) in an DHAM group animal. The arrowhead points to the injury site. i. Anti-aggrecan immunohistochemistry in DHAM + 15D-PGJ2-NC group animal. The arrowhead points to the injury site. open_in_new

table_chartTable I Comparison of the area at the site of the tracheal defect between groups, two by two.

Compared groups	p
Control x DHAM	<0.001
Control x DHAM+15D-PGJ2-NC	0.005
DHAM x DHAM+15D-PGJ2-NC	0.111

DHAM, Decellularized Human Amniotic Membrane; 15D-PGJ2-NC, 15-Deoxy-∆12,14ProstaglandinJ2 nanoparticles.

table_chartTable II Comparison of averages indices of histological findings and characterization of the inflammatory profile.

Histological Indexes	Average Index Inflammation
Control	+3.6 Chronic
DHAM	+2.8 Chronic
DHAM+15D-PGJ2-NC	+2.0 Subacute

DHAM, Decellularized Human Amniotic Membrane; 15D-PGJ2-NC, 15-Deoxy-∆12,14ProstaglandinJ2 nanoparticles.

table_chartTable III Comparison of the cartilaginous tissue (aggrecan) between groups, two by two.

Compared Groups	p
Control x DHAM	1
Control x DHAM+15D-PGJ2-NC	0.048
DHAM x DHAM+15D-PGJ2-NC	0.007

DHAM, Decellularized Human Amniotic Membrane; 15D-PGJ2-NC, 15-Deoxy-∆12,14ProstaglandinJ2 nanoparticles.

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[tfnI] DHAM, Decellularized Human Amniotic Membrane; 15D-PGJ2-NC, 15-Deoxy-∆12,14ProstaglandinJ2 nanoparticles.