Compatibility in pollen-pistil interaction of interspecific crossings with <i>Passiflora</i> spp.

SOARES, WELLINGTON S.; BRUCKNER, CLAUDIO H.; OTONI, WAGNER C.; OLIVEIRA, SILVIA P. DE; SANTOS, CARLOS EDUARDO M. DOS; KOEHLER, ANDREA D.

doi:10.1590/0001-3765202020180062

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AGRARIAN SCIENCES • An. Acad. Bras. Ciênc. 92 (suppl 1) • 2020 • https://doi.org/10.1590/0001-3765202020180062 linkcopy

Compatibility in pollen-pistil interaction of interspecific crossings with Passiflora spp.

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Intraspecies or interspecies crossings transfer relevant alleles between plants. However, some interspecies crossings involving Passiflora species impede ovule fertilization and the viable development of seeds. Thus, the purpose of this study was to verify the viability of interspecific crossings and monitor pollen tube development. The experiment had six species of Passiflora in the reciprocal crossings. Histochemical tests aimed to evaluate the percentage of intraspecies or interspecies crossings that resulted in fruit development and pollen tube development. Ovule fertilization and fruit development occurred in determined directions of crossings when controlling the female or male genitor, but only one case of reciprocal crossing had success. In crossings with no fruit development, histological analysis showed that some callus developed in the stigma and style, confirming unilateral and interspecies incompatibility in the genus Passiflora to some species and some directions of crossings.

Key words
interspecific hybridization; incompatibility; plant crossings; genetic breedings

P. gibertii (♀)	P. mucronata (♂)								Average
P. gibertii (♀)	22	23	24	25	26	27	28	NP	F	(%)
7	20	0	0	60	0	0	0	35	4	11
8	0	0	0	0	0	0	0	35	0	0
9	0	0	20	60	0	0	40	35	6	17
10	0	0	0	0	0	0	20	35	1	3
61	0	0	0	0	0	0	0	35	0	0
65	0	0	0	0	0	0	0	35	0	0
69	0	0	0	0	14	0	20	35	2	5
Average	3%	0%	3%	17%	3%	0%	11%	245	13	5

	P. gibertii (♂)								Average
P. mucronata (♀)	7	8	9	10	61	65	69	NP	F	(%)
22	0	0	0	0	0	0	0	35	0	0
23	0	0	0	0	0	0	0	35	0	0
24	0	0	0	0	0	0	0	35	0	0
25	0	0	0	0	0	0	0	35	0	0
26	0	0	0	0	0	0	0	35	0	0
27	0	0	0	0	0	0	0	35	0	0
28	0	0	0	0	0	0	0	35	0	0
Average	0%	0%	0%	0%	0%	0%	0%	245	0	0

Random -pollination			Number of seeds	Standard deviation
P. cincinnata			178.5	18.12
P. mucronata			89	7.26
P. gibertii			25	4.66
P. alata			191	9.86
P. edulis			330	16.08
Controlled crossings
♀		♂	Number of seeds	Standard deviation
P. gibertii	X	P. cincinnata	17.10	4.86
P. gibertii	X	P. mucronata	27.18	2.98
P. gibertii	X	P. alata	25	6.86
P. gibertii	X	P. edulis	9	4.88
P. alata	X	P. cincinnata	122	5.61
P. alata	X	P. mucronata	28	4.36
P. alata	X	P. edulis	120	6.34
P. edulis	X	P. cincinnata	180.12	8.57
P. edulis	X	P. mucronata	44	7.06

	P. mucronata (♂)								Average
P. alata (♀)	22	23	24	25	26	27	28	NP	F	(%)
11	0	0	0	0	0	0	0	35	0	0
12	0	0	0	0	0	0	0	35	0	0
13	0	0	0	0	0	0	0	35	0	0
16	0	0	0	0	0	0	0	35	0	0
17	0	14	0	0	0	0	0	35	1	3
89	0	0	0	0	0	0	0	35	0	0
94	0	20	0	0	0	0	0	35	1	3
Average	0%	5%	0%	0%	0%	0%	0%	245	2	1

	P. alata (♂)								Average
P. mucronata (♀)	11	12	13	16	17	89	94	NP	F	(%)
22	0	0	0	0	0	0	0	35	0	0
23	0	0	0	0	0	0	0	35	0	0
24	0	0	0	0	0	0	0	35	0	0
25	0	0	0	0	0	0	0	35	0	0
26	0	0	0	0	0	0	0	35	0	0
27	0	0	0	0	0	0	0	35	0	0
28	0	0	0	0	0	0	0	35	0	0
Average	0%	0%	0%	0%	0%	0%	0%	245	0	0

	P. mucronata (♂)								Average
P. edulis (♀)	22	23	24	25	26	27	28	NP	F	(%)
102	0	0	0	0	0	0	0	35	0	0
105	0	0	0	0	0	0	0	35	0	0
110	0	0	40	0	0	0	0	35	2	6
139	0	0	0	0	0	0	0	35	0	0
143	0	0	0	0	0	0	0	35	0	0
157	0	0	0	0	0	0	0	35	0	0
169	0	0	0	0	0	0	0	35	0	0
Average	0%	0%	6%	0%	0%	0%	0%	245	2	1

	P. edulis (♂)								Average
P. mucronata (♀)	102	105	110	139	143	157	169	NP	F	(%)
22	0	0	0	0	0	0	0	35	0	0
23	0	0	0	0	0	0	0	35	0	0
24	0	0	0	0	0	0	0	35	0	0
25	0	0	0	0	0	0	0	35	0	0
26	0	0	0	0	0	0	0	35	0	0
27	0	0	0	0	0	0	0	35	0	0
28	0	0	0	0	0	0	0	35	0	0
Average	0%	0%	0%	0%	0%	0%	0%	245	0	0

	P. alata (♂)							Average
P. gibertii (♀)	11	12	13	16	17	89	94	NP	F	(%)
7	0	0	0	0	0	0	0	35	0	0
8	0	0	0	0	0	0	0	35	0	0
9	0	0	0	0	0	0	0	35	0	0
10	0	0	0	0	40	0	0	35	2	6
61	0	0	0	0	0	0	0	35	0	0
65	0	0	0	0	0	0	0	35	0	0
69	0	0	0	0	0	0	0	35	0	0
Average	0	0	0	0	5	0	0	245	2	1
	P. gibertii (♂)							Average
P. alata (♀)	7	8	9	10	61	65	69	NP	F	(%)
11	0	0	0	0	0	0	0	35	0	0
12	0	0	0	0	0	0	0	35	0	0
13	0	0	0	0	0	0	0	35	0	0
16	0	0	0	0	0	0	0	35	0	0
17	0	0	20	0	0	0	0	35	1	3
89	0	0	0	0	0	0	0	35	0	0
94	0	0	0	0	0	0	0	35	0	0
Average	0	0	3	0	0	0	0	245	1	0.41

	P. cincinnata (♂)							Average
P. gibertii (♀)	2	3	4	5	18	19	50	NP	F	(%)
7	0	0	20	40	0	0	0	35	3	9
8	0	0	20	0	0	0	0	35	1	3
9	0	0	20	20	0	80	0	35	6	17
10	0	0	0	0	0	0	0	35	0	0
61	20	0	40	0	0	0	0	35	3	9
65	0	0	40	0	0	0	0	35	2	6
69	20	0	20	0	0	0	0	35	7	20
Average	6	0	37	9	0	11	0	245	22	9

	P. gibertii (♂)							Average
P. cincinnata (♀)	7	8	9	10	61	65	69	NP	F	(%)
2	0	0	0	0	0	0	0	35	0	0
3	0	0	0	0	0	0	0	35	0	0
4	0	0	0	0	0	0	0	35	0	0
5	0	0	0	0	0	0	0	35	0	0
18	0	0	0	0	0	0	0	35	0	0
19	0	0	0	0	0	0	0	35	0	0
50	0	0	0	0	0	0	0	35	0	0
Average	0	0	0	0	0	0	0	245	0	0

	P. cincinnata (♂)							Average
P. alata (♀)	2	3	4	5	18	19	50	NP	F	(%)
11	0	0	20	0	0	0	0	35	1	3
12	0	0	0	0	0	0	0	35	0	0
13	0	0	0	0	0	0	0	35	0	0
16	0	0	0	0	0	0	0	35	0	0
17	0	0	0	0	0	0	0	35	0	0
89	0	0	0	0	0	0	0	35	0	0
94	0	0	0	0	0	0	0	35	0	0
Average	0	0	3	0	0	0	0	245	1	0.41

	P. alata (♂)							Average
P. cincinnata (♀)	11	12	13	16	17	89	94	NP	F	(%)
2	0	0	0	0	0	0	0	35	0	0
3	0	0	0	0	0	0	0	35	0	0
4	0	0	0	0	0	0	0	35	0	0
5	0	0	0	0	0	0	0	35	0	0
18	0	0	0	0	0	0	0	35	0	0
19	0	0	0	0	0	0	0	35	0	0
50	0	0	0	0	0	0	0	35	0	0
Average	0	0	0	0	0	0	0	245	0	0

	P. cincinnata (♂)								Average
P. edulis (♀)	2	3	4	5	18	19	50	NP	F	(%)
102	0	0	0	0	20	0	0	35	1	3
105	0	0	20	0	0	20	0	35	2	6
110	0	20	0	0	0	0	0	35	1	3
139	0	0	0	0	40	0	0	35	2	6
143	0	0	0	0	0	0	0	35	0	0
157	0	0	0	0	0	0	100	35	5	14
169	0	0	0	0	0	0	40	35	2	6
Average	0	3	3	0	9	3	20	245	13	5

	P. edulis (♂)								Average
P. cincinnata (♀)	102	105	110	139	143	157	169	NP	F	(%)
2	0	0	0	0	0	0	0	35	0	0
3	0	0	0	0	0	0	0	35	0	0
4	0	0	0	0	0	0	0	35	0	0
5	0	0	20	0	0	0	0	35	1	3
18	0	0	0	0	0	0	0	35	0	0
19	0	0	0	0	0	0	0	35	0	0
50	0	0	0	0	0	0	0	35	0	0
Average	0	0	3	0	0	0	0	245	1	0.41

	P. edulis (♂)								Average
P. gibertii (♀)	102	105	110	139	143	157	169	NP	F	(%)
7	0	0	20	0	0	0	0	35	1	3
8	0	0	0	0	0	0	0	35	0	0
9	0	0	0	0	0	0	0	35	0	0
10	0	0	0	0	0	0	0	35	0	0
61	0	0	0	0	0	0	0	35	0	0
65	0	0	0	0	0	0	0	35	0	0
69	0	0	0	0	0	0	0	35	0	0
Average	0	0	3	0	0	0	0	245	1	0.41

	P. gibertii (♂)								Average
P. edulis (♀)	102	105	110	139	143	157	169	NP	F	(%)
102	0	0	0	0	0	0	0	35	0	0
105	0	0	0	0	0	0	0	35	0	0
110	0	0	0	0	0	0	0	35	0	0
139	0	0	0	0	0	0	0	35	0	0
143	0	0	0	0	0	0	0	35	0	0
157	0	0	0	0	0	0	0	35	0	0
169	0	0	0	0	0	0	0	35	0	0
Average	0	0	0	0	0	0	0	245	0	0

	P. edulis (♂)							Average
P. alata (♀)	102	105	110	139	143	157	169	NP	F	(%)
11	0	0	0	0	0	0	0	35	0	0
12	0	0	0	0	0	0	0	35	0	0
13	0	40	0	0	0	0	0	35	2	6
16	0	0	0	0	0	0	0	35	0	0
17	0	0	0	0	0	0	0	35	0	0
89	0	40	0	0	0	0	0	35	2	6
94	0	80	0	0	0	0	0	35	4	11
Average	0	23	0	0	0	0	0	245	8	3

	P. alata (♂)								Average
P. edulis (♀)	11	12	13	16	17	89	94	NP	F	(%)
102	0	0	0	0	0	0	0	35	0	0
105	0	0	0	0	0	0	0	35	0	0
110	0	0	0	0	0	0	0	35	0	0
139	0	0	0	0	0	0	0	35	0	0
143	0	0	0	0	0	0	0	35	0	0
157	0	0	0	0	0	0	0	35	0	0
169	0	0	0	0	0	0	0	35	0	0
Average	0	0	0	0	0	0	0	245	0	0

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[tfnI] NP: pollination number; F: fruit production; ♀: female genitor; ♂: male genitor.