Toxicological Evaluation of Anti-Scrapie Trimethoxychalcones and Oxadiazoles

An altered form of the cellular prion protein, the PrP or PrP, is implicated in the occurrence of the still untreatable transmissible spongiform encephalopathies. We have previously synthesized and characterized aromatic compounds that inhibit protease-resistant prion protein (PrP) accumulation in scrapie-infected cells. These compounds belong to different chemical classes, including acylhydrazones, chalcones and oxadiazoles. Some of the active compounds were non-toxic to neuroblastoma cells in culture and seem to possess drugable properties, since they are in agreement with the Lipinski ́s rule of 5 and present desirable pharmacokinetic profiles as predicted in silico. Before the evaluation of the in vivo efficacy of the aromatic compounds in scrapie-infected mice, safety assessment in healthy mice is needed. Here we used Swiss mice to evaluate the acute toxicity profile of the six most promising anti-prionic compounds, the 2,4,5-trimethoxychalcones (J1, J8, J20 and J35) and the 1,3,4-oxadiazoles (Y13 and Y17). One single oral administration (300 mg/kg) of J1, J8, J20, J35, Y13 and Y17 or repeated intraperitoneal administration (10 mg/kg, 3 times a week, for 4 weeks) of J1, J8 and J35, did not elicit toxicity in mice. We strongly believe that the investigated trimethoxychalcones and oxadiazoles are interesting compounds to be further analyzed in vivo against prion diseases.


INTRODUCTION
Prion diseases or transmissible spongiform encephalopathies (TSEs) are severe neurodegenerative disorders that still lack efficient treatment.An abnormal form of the cellular prion protein (PrP), the scrapie PrP (PrP Sc ), is involved in the establishment of TSEs (Prusiner 1998).PrP Sc is partially protease-resistant, poorly soluble in aqueous solvents and accumulates in its aggregated form in the brain of affected individuals.Most experimental approaches against TSEs focused either on inhibiting the conversion of PrP C to PrP Sc or on controlling the clearance of PrP Sc from infected cells and tissues (Kocisko et al. 2003, Kocisko and Caughey 2006, Rainov et al. 2007, Forloni et al. 2013, Cordeiro and Ferreira 2015).Several classes of aromatic or-CLAUDIA P. FIGUEIREDO et al. ganic compounds have been screened for these effects, such as quinolines, acridines, oxadiazoles, chalcones, 2-aminothiazoles, arylamides, among others (Doh-ura et al 2000, Korth et al. 2001, Koster et al. 2003, Cashman and Caughey 2004, Ghaemmaghami et al. 2010, Macedo et al. 2010, Sim 2012, Li et al. 2013, Ferreira et al. 2014).The majority of tests have used cultured mammalian cells infected with the scrapie form of PrP obtained from different sources (murine, hamster) (Kocisko et al. 2003, Kocisko and Caughey 2006, Sim 2012, Ferreira et al. 2014).Their ability to reduce PrP Sc (or PrP Res ) levels in vitro is usually evaluated using anti-PrP antibodies in proteinase-K treated cells.Although several of these compounds show low IC 50 values (nM range), only a few were able to cross the blood brain barrier (BBB) and/or were evaluated for their pharmacokinetic profile (Koster et al. 2003, Silber et al. 2013).Flupirtine, quinacrine, chlorpromazine, pentosan polysulfate (PPS) and doxycycline have even been tested in humans, and even though clinical improvement and marginally increase in the survival time (for PPS) were observed in some cases, none of them were able to reverse or even to halt the clinical course of the disease (Furukawa et al. 2002, Cashman and Caughey 2004, Otto et al. 2004, Martínez-Lage et al. 2005, Rainov et al. 2007, Tsuboi et al. 2009, Collinge et al. 2009, Forloni et al. 2013, Haïk et al. 2014).The investigation of drugs/phytotherapics already commercialized with other clinical indications has helped to overcome the toxicological limitations of studying new compounds and administering them to animal models and humans.However, newly developed molecules might possess better efficacy and safety compared to old ones and could represent an important step in the development of antiscrapie therapeutic strategies.Nevertheless, initial toxicological analysis and evaluation of the pharmacokinetic (PK) profile of these new substances are indeed needed.
We have recently identified a new panel of aromatic hetero and homocyclic compounds with anti-scrapie activity by screening in scrapie-infected neuroblastoma (ScN2a) cells (Ferreira et al. 2014, Cordeiro andFerreira 2015).Among ~ 50 active compounds, we identified four trimethoxychalcones (named J1, J8, J20, J35) and two oxadiazoles (Y13 and Y17) with IC 50 ~1 µM (Js) and ~10 µM (Ys), respectively, obtained after incubation with ScN2a cells for 4 days.In silico PK and physico-chemical predictions indicated that these compounds have promising drug characteristics, such as reduced mutagenicity, high permeation to the central nervous system and high oral bioavailability (Ferreira et al. 2014).One study has shown that the chalcones J8, J20 and J35 at 100 µM, reduced the viability of human K562 acute myeloid leukemia cells from 30-50 % (Costa et al. 2014).J1 inhibited the activity of cruzain from T. cruzi with an IC 50 of 100 µM (Borchhardt et al. 2010).However, even for the compounds that had different biological/ pharmacological activities, they did not match the potential anti-scrapie activity, as the IC 50 values obtained in ScN2a cells were significantly lower than those reported (Borchhardt et al. 2010, Costa et al. 2014).Therefore, we do not expect that the compounds are at a risk of presenting unwanted pharmacological side effects during their preclinical and clinical trials.
Here, we assessed the safety of compounds J1, J8, J20, J35, Y13 and Y17 (Fig. 1) by evaluating their toxic effects after acute oral administration or following repeated intraperitoneal administration in mice.The development of behavioural alterations (Hippocratic test), food and water intake, were evaluated.In addition, the effect of the compounds on lethality was investigated during the first 24 h and for 14 or 28 days in order to assess the possible alterations related to the course of treatment.Animals were sacrificed at the end of the observation period and assessment of macroscopic alterations in several organs and/or glands was performed.
None of the investigated compounds caused death of the mice at the administered dose and no macroscopic alterations were seen in the selected organs or glands following oral administration.Our results indicate that LD 50 is higher than 300 mg/kg for all studied compounds, and that oral administration of 2,4,5-trimethoxychalcones and 1,3,4-oxadiazoles is safe in doses of up to 300 mg/kg.In addition, they can also be considered safe for systemic administration, since only one mouse treated with J8 and one with J35 (both intraperitoneal administration) showed minor lung bleeding, kidney discoloration and increased relative weight of the heart, when compared with the control group.This toxicological evaluation provides preliminary information for in vivo studies in scrapie-infected murine models.

AnimAls
Acute oral toxicity experiments were conducted on 36 male and 38 female adult Swiss mice (6-8 weeks old, 22-27 g), and repeated intraperitoneal doses were conducted on 24 female adult Swiss mice (8-12 weeks old, 30-35 g).The animals were purchased from Central Biotery of Fundação Oswaldo Cruz (FIOCRUZ -Rio de Janeiro/ RJ).Animals were housed in the vivarium at the School of Pharmacy, CCS, UFRJ.Animals were housed in clear microisolator cages (40x32x17 cm) (ALESCO, Campinas, Brazil), with controlled temperature (22 ± 2 °C), humidity between 60-80 % on a 12-h light/dark cycle, with food and water ad libitum.All experiments were conducted in accordance with the ethical principles set forth by the Brazilian School of Animal Experimentation (COBEA 1991) and with the approval of the Ethics Committee for Animal Experimentation (CEUA) of the Federal University of Rio de Janeiro (Protocol FARMACIA07-04/16).Prior to experimentation, animals were individually identified in order to evaluate behaviour and body weight during the period of study, as well as to evaluate organ weight at the end of the experiments.

In silico prediCtion of toxiCologiCAl properties
Physicochemical and toxicity properties of compounds J1, J8, J20, J35, Y13 and Y17 were predicted with software modules from ACD/ Labs Percepta Platform, version 12.01, Advanced Chemistry Development, Inc., Toronto, On, Canada (www.acdlabs.com 2014) (ttp://www.acdlabs.com/products/percepta/predictors.php) from the uploaded three-dimensional structure of the compounds.Software components provided by ACD/Labs (from PhysChem Modules and Toxicity Modules) were used to predict aqueous solubility, toxicity category and LD 50 values for the selected compounds.We used the LD 50 module that predicts LD 50 values (mg/kg) for rats and mice according to various routes of administration, and the Toxicity Categories module that classifies compounds into OECD categories for acute oral toxicity.OECD assigns chemicals to one of the five Oral Acute Toxicity Hazard Categories according to the LD 50 values after oral administration to mice, which are: V, 2000 to 5000 mg/kg (may be harmful if swallowed); IV, 300 to 2000 mg/kg (harmful if swallowed); III, 50 to 300 mg/kg (toxic if swallowed); II, 5 to 50 mg/kg (fatal if swallowed); I, < 5 mg/kg (fatal if swallowed).Predictions are provided as a list of probabilities that the compound's LD 50 (rat, oral route) would exceed the cut-off values separating different categories.On the basis of these values, the software selects the most probable OECD Hazard categories for that compound.For both solubility and LD 50 calculation a reliability index (RI) value is provided that represents a quantitative evaluation of prediction confidence.In general, RI values below 0.3 indicate that the respective prediction is not reliable.
prepArAtion of the J1, J8, J20, J35, Y13 And Y17 Compounds Stocks solutions were prepared according to the proposed doses (based on in silico LD 50 prediction) and considering the estimated water solubility of the compounds.Stock solutions of compounds were prepared by solubilising the compound in 0.9 % NaCl supplemented with Tween 80 at 8 % (for i.p. administration) or 10 % (for oral administration).
orAl ACute toxiCitY In order to investigate the possible acute toxicity of the trimethoxychalcones (J1, J8, J20, J35) and oxadiazoles (Y13 and Y17), groups of 10 to 12 mice (male and female) were starved for 4 h and J1, J8, J20, J35, Y13 or Y17 (300 mg/kg) or vehicle was administered orally.Control groups received vehicle (0.9 % NaCl containing 10 % Tween 80, 10 mL/kg, p.o.).After oral administration of vehicle or the compounds, animals were arranged in groups and kept in clear microisolator cages for 14 days.Parameters indicative of toxicity, such as abdominal constriction, ptosis, piloerection, tremors, paralysis, tremor, reduction of body tonus, convulsions (seizures), corneal reflex, touch response, tail grip, and mortality were evaluated 0.25, 0.5, 1, 2, 4, 8 and 24 h after treatment (Brandão 2004, OECD 2001).The dose selection was based on OECD/ OCDE Guideline 423, which suggests that when there is no information on a substance to be tested, for animal welfare reasons, it is recommended to use the starting dose of 300 mg/kg body weight (OECD 2001).The possible influence of acute administration of compounds on food and water intake, weight gain, as well as on some vital organs was assessed.Thus, animals were kept in microisolator cages and on the 1 st , 4 th , 7 th , 9 th , 11 th and 14 th day after administration of J1, J8, J20, J35, Y13 and Y17 (300 mg/kg) or vehicle, body weight, food and water intake were evaluated.On the 14 th day of observation, animals were anesthetized and sacrificed by cervical displacement and subjected to laparotomy, which allowed macroscopic observation (general aspect, colour and weight) of organs and/or glands, such as: heart, lung, liver, kidney, spleen, stomach, testis, ovary and uterine tubes, salivary gland, adrenal gland, thymus, mesenteric lymph node and encephalon.

sYstemiC toxiCitY
To analyze the possible systemic toxicity of the trimethoxychalcones, mice were grouped in microisolator cages (n = 6), and received J1, J8 or J35 (10 mg/kg), administered via intraperitoneal (i.p.) route three times a week for four weeks.
The control group received vehicle (0.9 % NaCl containing 8 % Tween 80, 10 mL/kg, i.p.) at the same intervals.Parameters indicative of toxicity, as well as body weight and relative weight of some organs were assessed as described for the oral acute toxicity experiments.Mice were evaluated every other day and sacrificed after 28 days.Macroscopic observation of organs was performed as described for the oral acute toxicity experiments.
stAtistiCAl AnAlYses Results are expressed as mean ± standard error mean or as mean with 95 % of confidence interval.Statistical analysis of results was performed using one-or two-way analysis of variance (ANOVA) followed by post-hoc Newman-Keuls test when appropriate.For absolute body weight comparison, two-way ANOVA with repeated measures was performed.Differences from the control group were considered to be significant when P < 0.05.

RESULTS
One of the limitations to explore new compounds as possible drug candidates is the lack of pharmacokinetic and toxicological information, which limits their application in clinical studies.We have previously described novel chalcones and oxadiazoles with potent anti-scrapie activity, as seen by the clearance of protease-resistant PrP (PrP Res ) from prion scrapie infected cells (Ferreira et al. 2014).Here we describe a preliminary acute toxicity evaluation of the compounds that had low IC 50 values and suitable PK properties predicted in silico.
Prior to proceeding with the toxicological analysis, acute toxicity and aqueous solubility were predicted in silico for the compounds that: were active in the dot-blot assay (clearance of PrP Res from ScN2a cells), were non-toxic to N2a cells, had good PK characteristics and were predicted to directly interact with PrP C (Ferreira et al. 2014).CLAUDIA P. FIGUEIREDO et al.
The software modules from ACD/Labs Percepta Platform were used.All compounds were predicted to have low solubility in water, as expected from their chemical structure.The obtained results are summarized in Table I.Prediction of aqueous solubility for the oxadiazoles Y13 and Y7 was not reliable because RI values were very low (0.13 and 0.32), probably due to the lack of experimental values for compounds with similar structures.However, they were partially solubilized in Tween 80 from 8 to 10 %.We used two software components related to acute toxicity provided by ACD/Labs Acute Toxicity predictor.Compounds from the J series (2,4,5-trimethoxychalcones) were predicted as being less toxic than compounds from the Y series (1,3,4-oxadiazoles), according to the classification into OECD toxicity categories and the calculated LD 50 values (Table I).
The compounds had anti-scrapie activity in the dot-blot assay at IC 50 values ranging from 1 to 10 µM (from ~0.4 to 4.0 mg/L) (Ferreira et al. 2014); these values, along with the predicted LD 50 and solubility values reported here, guided us to select the best dosing regimen for the toxicology studies.Our toxicity studies were performed in accordance with the specifications of the Agência Nacional de Vigilância Sanitária/Brazilian Sanitary Agency (ANVISA) and The Organisation for Economic Co-operation and Development (OECD).For acute toxicity tests, ANVISA recommends the use of a dose which corresponds to at least tenfold the clinical exposure dose (ANVISA 2010).Since our compounds were effective from 1 to 10 µM, the maximum dose would be 4.0 mg/kg and the acute toxicity test should be performed using a dose of at least 40 mg/kg.However, to work with a wide safety margin and based on OECD Guideline 423 (OECD 2001) we used a single dose of 300 mg/ kg.Regarding the multiple dose toxicity study, ANVISA recommends a dose lower than 1,000 mg/ kg/day.For 14 days we investigated the acute toxicity of J1, J8, J20, J35, Y13, and Y17 (diluted in 10 % Tween 80), administered orally at a single dose of 300 mg/kg to 74 Swiss mice (36 male and 38 female, 10 to 12 animals per group).Acute treatment with all compounds (at 300 mg/kg) or vehicle, administered orally, caused 10 % of lethality in mice, which was not significantly different from the control group (Table II).In addition, acute treatment with the compounds or vehicle did not induce behavioral alterations indicative of acute toxicity in either male or female mice, such as piloerection, tremors, reduction of body tonus, abdominal constrictions, convulsions (seizures), touch response, tail grip or corneal reflex, when compared with the control group (vehicle) (Table III, IV and V).
Food and water intake were not modified significantly upon acute oral treatment with the compounds or vehicle compared with the control group (Table VI).Corroborating these findings, the treatment with J1, J8, J20, J35, Y13 and Y17 (300 mg/kg) or vehicle, administered orally to mice, did not significantly change absolute body weight of animals when compared with the control group (Fig. 2).Three animals treated     with Y17 had diarrhea in the first hour following administration.In addition, mice which received acute treatment with the compounds at 300 mg/ kg, administered orally, showed no important macroscopic alterations considering aspect and color of the selected organs and glands (heart, lung, liver, kidney, spleen, stomach, testis, ovary and uterine tubes, salivary gland, adrenal gland, thymus, mesenteric lymph node and encephalon), when compared with the control group (vehicle), all of which were evaluated at the 14 th day of study.Furthermore, mice acutely treated orally with J1, J8, J20, J35, Y13 and Y17 (300 mg/kg), did not show significant differences in either relative or absolute weight of heart, lung, kidney, spleen, stomach, salivary gland, adrenal gland, thymus and encephalon when compared with the control group, all of which were evaluated at the 14 th day of study (Table VII).Only treatment with J1 resulted in significant increase in the relative weight of the testis (Table VII).
Next, we evaluated the systemic effects of intraperitoneal administration of selected compounds from the J series (J1, J8 and J35).
Only three compounds were assayed in this set of experiments because limited amounts of the other compounds were available to complete the assay.
In this set of experiments, mice were treated with multiple doses (10 mg/kg, 3 times a week, i.p.), as this will be the probable dosing regimen for future in vivo assays with scrapie-infected mice.Moreover, at this selected dose, the compound concentration will be above the estimated IC 50 values (Ferreira et al. 2014).When evaluated for 28 days, none of the tested compounds induced mouse mortality (data not shown) and no significant alterations in body weight were observed in mice treated with J1, J8 or J35 (10 mg/kg, 3 times a week, i.p., for 28 days) when compared with control mice (Fig. 3).Macroscopic evaluation of the selected organs and glands (heart, lung, liver, kidney, spleen, stomach, adrenal gland, and encephalon) demonstrated no alterations of aspect and color in mice treated with J1 (10 mg/kg, 3 times a week, i.p.), evaluated at the 28 th day of study, when compared with the control group (vehicle).Similar results were found for J8 and J35; however, one mouse treated with J8 and one with J35 (10 mg/kg, 3 times a week, i.p.) showed minor lung bleeding, and one mouse treated with J35 presented kidney discoloration.
In addition, systemic treatment with J1, J8, or J35 (10 mg/kg, 3 times a week, i.p.) for 28 days was not capable of inducing significant alterations in absolute and relative weight of lung, liver, kidney, spleen, stomach, adrenal gland, and encephalon, when compared with the control group.However, J35 caused significant increase in the relative weight of heart of mice following i.p. treatment (10 mg/kg, 3 times a week, during 4 weeks, i.p.) (Table VIII).
In conclusion, analyses of results demonstrated that acute oral administration of J1, J8, J20, J35, Y13 and Y17, and intraperitoneal repeated doses of J1, J8 and J35 showed good tolerability and had no acute toxic effects in mice, when evaluated on several behavioral parameters.The six compounds showed low acute toxicity and did not cause alterations in body mass or food and water intake in mice.In addition, the acute oral administration of these compounds did not lead to important macroscopic alteration concerning the aspect, color and size of organs and/or glands, such as heart, lung, liver, kidney, spleen, stomach, ovary and uterine tubes, salivary gland, adrenal gland, thymus, mesenteric lymph node and encephalon, when compared with organs of control mice.J1, J8 and J35 also indicated being safe when administered systemically, with only one mouse treated with J8 and one with J35 showing minor lung bleeding, kidney discoloration and increased relative weight of the heart when compared with the control group.

DISCUSSION
Chalcones and oxadiazoles are chemical classes of compounds with several biological activities, but to date only our previous report revealed their putative anti-scrapie activity (Ferreira et al. 2014).Particularly, 1,3,4-oxadiazoles with different substituents have been assayed for antiinflammatory, anti-tumor, analgesic, anti-viral and antimicrobial activities (Omar et al. 1996, Sun et al. 2013), being considered privileged structures in medicinal chemistry (Rane et al. 2013).Acute toxicity studies of substituted 1,3,4-oxadiazoles that have anti-inflammatory and analgesic activities showed LD 50 values of ~1000 mg/kg after i.p. administration to mice (Omar et al. 1996).As we did not reach such dose in our study, we can only suppose that Y13 and Y17 will also have high LD 50 values.However, we are dealing with different oxadiazole derivatives, what can completely alter the PK profile.Recently, anti-bacterial, anti-fungal and anti-tubercular activities were described for 4-nitropyrrole-based 1,3,4-oxadiazoles.Their IC 50 values for cytotoxicity in mammalian VERO cells ranged from 100-700 µM (Rane et al. 2013), much higher than the minimum inhibitory concentrations (MIC).Our previous study also showed that Y13 and Y17 are non-toxic to cultured mammalian cells at and above the effective anti-scrapie concentration (Ferreira et al. 2014).
Besides having several known pharmacological activities, the synthesis of chalcones is rapid, low cost and normally with high yields, which renders this class of compounds promising for drug development.Other 3,4,5-trimethoxychalcone analogues presented potent activity against H. pylori (Lai et al. 2010) and inhibited both nitric oxide production and tumor cell proliferation (Rao et al. 2009).The 3,4,5-trimethoxyphenyl ring is increasingly associated with antitumor activity of several chalcone derivatives.Although differing in the methoxy position, the 2,4,5-trimethoxychalcones investigated here (J8, J20 and J35) also had anti-proliferative activity against K652 leukemia cells (Costa et al. 2014).However, J8 and J20 reduced cell viability in only 30-35 %, and J35 to 50 % at 100 µM.J1 had no significant activity (Costa el al. 2014).Cytotoxicity against non-tumoral cell lines was investigated by us, and what we found is that the chalcones were not toxic to N2a cells in concentrations of up to 25 µM (Ferreira et al. 2014).Nonetheless, it remains to be confirmed whether these compounds are safe to non-tumoral cells at higher concentrations.
Regarding the ability to cross the BBB, there is lack of in vivo PK studies with the chalcones and oxadiazoles investigated here.A trimethoxychalcone derivative was detected in implanted glioblastoma tumors and in the brain of i.p. treated mice, showing that at least this specific chalcone crosses the BBB (Boumendjel et al. 2009).Previously, we used a computational approach to predict some pharmacokinetic parameters that helped us select the most promising compounds to assay in vivo.Our in silico analysis indicated that the fraction of J1, J35, Y13 and Y17 that reaches circulation after oral administration is theoretically expected to be higher than 70 %, while for J8 and J20 it stands between 30 and 70 %.These compounds were also classified as sufficiently CNS-permeable to be active in the CNS.These ADMET predictions do not substitute in vivo evaluation, but give us preliminary information on the potential drugability of these compounds (Cordeiro and Ferreira 2015).
All compounds were predicted to have low solubility in water (Table I), what renders them prone to cross the BBB.However, we still have not evaluated (in vitro or in silico) binding to plasma proteins, a feature that compromises permeation to biological membranes, even for lipophilic compounds.Prediction of toxicity indicated that all compounds from the J series are less toxic than compounds from the Y series (Table I).We were not able to calculate LD 50 values for the compounds, as oral and i.p. administration of both Ys and Js as single or multiple doses did not cause death of the treated mice.Consequently, although the in silico toxicity prediction supported the in vivo results for the chalcones, we could not correlate the LD 50 prediction for compounds of the Y series (Table I) to the obtained in vivo results.
Sub-acute and chronic toxicity studies are needed to provide data on genotoxicity, mutagenicity, and on long-term effects in organs and glands and overall animal behavior.However, the acute toxicity results presented here show that at least the compounds are safe regarding oral and i.p. administration at the investigated doses.Previous data showing the pharmaceutical importance of the 1,3,4-oxadiazole and trimethoxychalcone scaffolds (Sun et al. 2013, Rane et al. 2013, Omar et al. 1996, Costa el al. 2014, Ferreira et al. 2014), together with our in vivo acute toxicity results strongly indicate that J1, J8, J20, J35, Y13 and Y17 are interesting compounds for further investigation as anti-scrapie drugs or for other activities.Further structural modifications might also generate novel compounds with low toxicity and high potency.

Figure 2 -
Figure 2 -Effect of acute treatment with J1, J8, J20, J35, Y13 and Y17 (300 mg/kg) or vehicle, administered orally as a single dose, on absolute body weight of (A) male and (B) female mice.Each value represents mean of 9-12 animals and vertical lines represent S.E.M. Two-way ANOVA with repeated measures.

Figure 3 -
Figure 3 -Effect of repeated treatment with J1, J8, J35 (10 mg/kg) or vehicle, administered intraperitoneally for 28 days (3 times a week), on absolute body weight of female mice.Each value represents mean of 6 animals and vertical lines represent S.E.M. Two-way ANOVA with repeated measures shows no difference between the groups.

TABLE I Prediction of aqueous solubility and acute toxicity of compounds.
a Qualitative aqueous solubility predicted with PhysChem Module of ACD/Labs.b In accordance to OECD classification of compounds into one of five OECD categories for acute oral toxicity (Acute Toxicity Prediction Module).c Prediction of LD 50 values for mice according to various routes of administration (Acute Toxicity Prediction Module); RI values for J compounds were > 0.4 and for Y > 0.3.

TABLE VI Effect of acute treatment with J1, J8, J20, J35, Y13 and Y17 (300 mg/kg) or vehicle, after oral administration in males and females, on changes in the average of food and water intake evaluated at the end of treatment.
Values represent the variation of mean solid (g/animal/day) and liquid (mL/animal/day) intake of 9-12 animals ± standard error mean.Two-way ANOVA with repeated measures shows no difference between the groups.