Endophytic interaction of <i>Bacillus</i> sp. in micropropagated banana plantlets

Abstract: The banana tree is associated with different species of endophytic bacteria that can stimulate plant growth. However, further studies are needed to better understand the relationships between this group of bacteria and the host plant. The objective of this study was to investigate the localization of the EB-40 (Bacillus sp.) through anatomical and ultrastructural analyses in micropropagated banana plantlets. The results demonstrated the effective colonization of the EB-40 isolate in the intercellular and intracellular spaces, as well as in the rhizosphere region. The wall of endophytic bacteria contains calcium and nitrogen. The EB-40 isolate was also observed to associate with the plasma membrane and cell wall. These results further our understanding of the mechanisms involved in the colonization of plant cells by endophytic bacteria in micropropagated banana plantlets.

Key words
Endophytic bacteria; Musa spp.; light microscopy; transmission electron microscopy.

Authorship

JOSIELE S. ROCHA

Programa de Pós-Graduação em Produção Vegetal no Semiárido, Universidade Estadual de Montes Claros, Rua Reinaldo Viana, 2650, 39400-000 Janaúba, MG, Brazil Universidade Estadual de Montes ClarosBrazilJanaúba, MG, BrazilPrograma de Pós-Graduação em Produção Vegetal no Semiárido, Universidade Estadual de Montes Claros, Rua Reinaldo Viana, 2650, 39400-000 Janaúba, MG, Brazil

SILVIA NIETSCHE

Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Avenida Universitária, 1000, Bairro Universitário, 39404-547 Montes Claros, MG, Brazil Universidade Federal de Minas GeraisBrazilMontes Claros, MG, BrazilInstituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Avenida Universitária, 1000, Bairro Universitário, 39404-547 Montes Claros, MG, Brazil

http://orcid.org/0000-0002-8188-3057

MARLON C.T. PEREIRA

MERCADANTE-SIMÕES MARIA O.

Programa de Pós-Graduação em Biotecnologia, Universidade Estadual de Montes Claros, Campus Professor Darcy Ribeiro, s/n, 39401-089 Montes Claros, MG, Brazil Universidade Estadual de Montes ClarosBrazilMontes Claros, MG, BrazilPrograma de Pós-Graduação em Biotecnologia, Universidade Estadual de Montes Claros, Campus Professor Darcy Ribeiro, s/n, 39401-089 Montes Claros, MG, Brazil

RAYANE C. SANTOS

ADELICA A. XAVIER

Correspondence to: Silvia Nietsche E-mail: silvia.nietsche@gmail.com ORCid: https://orcid.org/0000-0002-8188-3057

AUTHOR CONTRIBUTIONS

SN, MCTP and MOMS conceived and designed research. JSR, RCS and AAX conducted experiments. MCTP, SN, RCS and MOMS analyzed data. SN, MOMS, JSR and AAX wrote the manuscript. All authors read and approved the manuscript.

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (3)

Thumbnail

Figure 1
Morphology of the root system of a banana tree plantlet (cv. Prata-Anã) cultured in vitro in association with endophytic bacteria (Bacillus sp. isolate EB-40) (a, b and c). am, apical meristem; rz, rhizome.

Thumbnail

Figure 2
Musa acuminata root (‘Prata-Anã’ AAB) cultivated in vitro and colonized by the isolate EB-40 (Bacillus sp.). Cross section of the median region. (a) Overview. (b) Detail of box B, marked in a. Unicellular, elongated root hairs with lignified walls. Biserial, pavimentous epidermis, with lignified walls. Outer layers of the cortex with small, bulky cells. Retracted protoplast. (c) Detail of box C, marked in a. Region of the aerenchymatous internal cortex. Endoderm with cells of suberized walls in all its extension. (d) Rod-shaped Bacillus sp. isolate EB-40 cells joined in rows of two to three cells. (e-f) A mucilaginous, fibrillar material in the rhizoplane and inside the root hairs. Caption: co: cortex; cp: cytoplasm; en: endoderm; ep: epidermis; fm: fibrillar material; ic: internal cortex; oc: outer cortex; tr: trichome; vs: vascular system. The arrows indicate the location of the bacteria.

Thumbnail

Figure 3
Ultrastructure of cortical cells of Musa acuminata (Prata-Anã AAB) grown in vitro and colonized by endophytic bacteria. (a) (e) (f) (i) Bacillus sp. (EB-40 Isolate) present in the intracellular medium. Cells of the protoplast collapsed, retracted and separated from the plasma membrane of the cellulosic wall; (b) Detail of box B, labeled in a. Association of the EB-40 isolate with the plasma membrane; (c) detail of box C, labeled in a. The thick wall and heterogeneous cytoplasm of the endophyte; (d) Detail of box D, labeled a. Presence of mucilaginous material involving endophytes; (g) Detail of box g, labeled in e. Wall of EB-40 isolate with calcium; (h) Detail of box h, labeled in e. Wall of isolate EB-40 with nitrogen; (j) Detail of box j, marked in i. Intimate association of the EB-40 isolate with the cellulosic wall; (k) Association of the EB-40 isolate with the cellulosic wall; (l) Detail of box l, labeled in k. The EB-40 isolate traversing the cell wall. Caption: cw: cellulosic wall; ew: endophytic wall; me: membrane; pr: protoplast.

Figure 1 Morphology of the root system of a banana tree plantlet (cv. Prata-Anã) cultured in vitro in association with endophytic bacteria (Bacillus sp. isolate EB-40) (a, b and c). am, apical meristem; rz, rhizome.

Figure 2 Musa acuminata root (‘Prata-Anã’ AAB) cultivated in vitro and colonized by the isolate EB-40 (Bacillus sp.). Cross section of the median region. (a) Overview. (b) Detail of box B, marked in a. Unicellular, elongated root hairs with lignified walls. Biserial, pavimentous epidermis, with lignified walls. Outer layers of the cortex with small, bulky cells. Retracted protoplast. (c) Detail of box C, marked in a. Region of the aerenchymatous internal cortex. Endoderm with cells of suberized walls in all its extension. (d) Rod-shaped Bacillus sp. isolate EB-40 cells joined in rows of two to three cells. (e-f) A mucilaginous, fibrillar material in the rhizoplane and inside the root hairs. Caption: co: cortex; cp: cytoplasm; en: endoderm; ep: epidermis; fm: fibrillar material; ic: internal cortex; oc: outer cortex; tr: trichome; vs: vascular system. The arrows indicate the location of the bacteria.

Figure 3 Ultrastructure of cortical cells of Musa acuminata (Prata-Anã AAB) grown in vitro and colonized by endophytic bacteria. (a) (e) (f) (i) Bacillus sp. (EB-40 Isolate) present in the intracellular medium. Cells of the protoplast collapsed, retracted and separated from the plasma membrane of the cellulosic wall; (b) Detail of box B, labeled in a. Association of the EB-40 isolate with the plasma membrane; (c) detail of box C, labeled in a. The thick wall and heterogeneous cytoplasm of the endophyte; (d) Detail of box D, labeled a. Presence of mucilaginous material involving endophytes; (g) Detail of box g, labeled in e. Wall of EB-40 isolate with calcium; (h) Detail of box h, labeled in e. Wall of isolate EB-40 with nitrogen; (j) Detail of box j, marked in i. Intimate association of the EB-40 isolate with the cellulosic wall; (k) Association of the EB-40 isolate with the cellulosic wall; (l) Detail of box l, labeled in k. The EB-40 isolate traversing the cell wall. Caption: cw: cellulosic wall; ew: endophytic wall; me: membrane; pr: protoplast.

How to cite

copy

Academia Brasileira de Ciências Rua Anfilófio de Carvalho, 29, 3º andar, 20030-060 Rio de Janeiro RJ Brasil, Tel: +55 21 3907-8100 - Rio de Janeiro - RJ - Brazil
E-mail: aabc@abc.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Endophytic interaction of <i>Bacillus</i> sp. in micropropagated banana plantlets

PlumX

Mensagem

[1] Correspondence to: Silvia Nietsche E-mail: silvia.nietsche@gmail.com ORCid: https://orcid.org/0000-0002-8188-3057