Hydrolysis of macauba kernel oil: ultrasound application in the substrates pre-emulsion step and effect of the process variables

The main goal of the present work was to evaluate the application of ultrasound as a previous step to promote the substrates pre-emulsion in the hydrolysis reaction of macauba kernel oil (MKO). The ultrasound effect on the substrates pre-emulsion was evaluated on the free fatty acid (FFA) content, as well as the process variables (reaction time, percentage of catalyst Lipozyme® RM IM, and buffer solution). Reactions carried out with the substrates pre-emulsion presented higher FFA production, up to a 40 wt% increase in 1 hour of reaction, yielding 80 wt% of FFAs in 8 hours. The use of catalyst in the reaction medium, from 5 to 15 wt%, favored the FFAs production in 2 hours of reaction. Addition of 25 to 100 wt% of buffer solution led to 86 wt% of FFAs in 4 hours of reaction. Enzyme recycling resulted in a slight decrease in the FFA content, although the catalyst had maintained 85% of its initial activity after 30 h of use. Therefore, the ultrasound pre-emulsion previous step allowed a more efficient hydrolysis reaction of MKO, leading to an increase of up to 40 wt% on the FFA content, when compared to the hydrolysis without such step.

Key words
enzymatic catalysis; free fatty acid; hydrolysis; ultrasound