Feeding and reproductive patterns of Astyanax intermedius in a headwater stream of Atlantic Rainforest

An Acad Bras Cienc aabc Anais da Academia Brasileira de Ciências An. Acad. Bras. Ciênc. 0001-3765 1678-2690 Academia Brasileira de Ciências RESUMO Neste trabalho nós determinamos a composição da dieta, periodicidade reprodutiva e fecundidade de Astyanax intermedius em um riacho de cabeceira de um Parque Estadual de Mata Atlântica. Avaliamos também a influência da chuva, temperatura da água e tamanho do peixe na amplitude e sobreposição de nicho. As amostragens foram realizadas mensalmente ao longo de um ano no riacho Ribeirão Grande, sudeste do Brasil. A dieta consistiu de 31 itens, com igual contribuição de itens alóctones e autóctones. As fêmeas foram maiores do que os machos e os comprimentos médios de primeira maturação foram de 4,44 cm e 3,92 cm, respectivamente. Com base nos 212 pares de ovários analisados, o número de ovócitos por fêmea variou de 538 a 6727 (média = 2688,7). A amplitude e a sobreposição de nicho não foram relacionadas à chuva e temperatura da água, e somente a amplitude de nicho aumentou com o tamanho do peixe, sugerindo que conforme o crescimento, mais itens são incluídos na dieta. Nossos resultados sugerem que A. intermedius apresenta um comportamento típico de espécie oportunista, o que pode explicar a prevalência desta espécie em diversos riachos de cabeceiras isolados de Mata Atlântica onde os recursos alimentares são abundantes e distribuídos ao longo do ano. INTRODUCTION In tropical riverine environments, where diversity of food is high and highly variable, there is a predominance of generalist and opportunistic fish species, reflecting the wide trophic adaptability of teleostei (Abelha et al. 2001). Research addres­sing variation in feeding habitats enables understanding how spatial and temporal changes in environmental factors affect the structure of fish populations (Esteves and Aranha 1999, Barreto and Aranha 2006). Another dimension of fish ecology is related to life history and reproductive patterns. Periodicity, reproductive effort and location of spawning habitats for example, are important for the understanding of how fish strive in specific environments, and which conservation actions are needed to preserve species and ecosystemic functions (Garutti 1989, Braga et al. 2006). Headwater streams are inhabited by small-sized fish species, in general with restrict geographical distribution and high endemism (Castro and Menezes 2001). The occurrence and distribution of fish in small streams relates to the abundance of alimentary resources (Angermeier and Karr 1983) and the riparian vegetation is an important source of energy and material, providing the necessary conditions for fish feeding (ex. terrestrial invertebrates) and reproduction (ex. shade) (Wootton 1992,Henry et al. 1994). In addition, the riparian vegetation contributes to particulate organic material that is processed by aquatic invertebrates that are ingested by several fish species (Gregory et al. 1991, Esteves and Aranha 1999, Pusey and Arthington 2003). Among small-sized fishes in Neotropic, the species within the "complexAstyanax scabripinnis" are restricted to headwaters of small streams (Britski 1972, Caramaschi 1986, Kavalco and Moreira-Filho 2003). This species complex was defined based on morphologic and karyotype variation (Moreira-Filho and Bertollo 1991) and seems to have evolved independently in different hydrographic basin, or even within a same hydrographic system, from isolated populations. Bertaco and Lucena (2006) named 15 species. Among them, A. intermedius Eigenmann, 1908 is distributed in the Paraíba do Sul River basin and coastal rivers of the state of Rio de Janeiro, Brazil (Lima et al. 2003). Most knowledge on A. intermedius relies on cytogenetic studies (Kavalco and Moreira-Filho 2003, Kavalco et al. 2004, 2007) whereas much of its life history and feeding behaviour remains to be elucidated. Besides its generalist behaviour, Souza et al. (2015) showed evidences for trade-offs between energy assimilation and reproductive effort of A. intermedius. Although males had constant reproductive effort throughout the year, females reproductive effort increased with water temperature, which resulted in a depleted body condition. Gomiero et al. (2012) also verified that part of this population had small reproductive success because some individual are parasitized by an isopod that attaches on to fish tongue. These fishes had a significant depletion on body condition and gonad development probably due to a reduced capacity of feeding. Thus, even for generalist species such as A. intermedius, the analysis of basic biological information provides insights on how environment affects population structure. In this paper, we determined diet composition ofA. intermedius in a headwater stream of a State Park of Atlantic rainforest and described information on reproductive periodicity and fecundity. We also evaluated if niche width and niche overlap are related to rainfall, water temperature and fish size. MATERIALS AND METHODS Study Region and Environmental Data Sampling was conducted monthly between January and December 2004 in the Ribeirão Grande stream (23o26' S, 45o14' W), a headwater stream at 757 m elevation located inside the environmental protection area "Parque Estadual da Serra do Mar (PESM)", Santa Virginia Unit, southeastern Brazil. The sample stretch had clear water surrounded by natural vegetation and streambed composed by sand and rocks. Ribeirão Grande is a 4th order stream (Strahler 1952) with a drainage area of 4.59 km2. It is a tributary of the Paraitinga river which originates the Paraíba do Sul river basin, one of the most impacted basins of southeast Brazil that crosses the states of São Paulo, Minas Gerais and Rio de Janeiro towards the Atlantic Ocean. Most park area is covered by pristine Atlantic rainforest with discontinuous patches of recovering native vegetation, abandoned eucalypt plantation, and small rural areas. The relief is predominantly mountainous with steep valleys characterizing the landscape. The tops of ridges are between 960 and 1160 m in the core park area, but reach a maximum of 1585 m elevation in the Ribeirão Grande stream subbasin (São Paulo 1998). Climate in the region is humid subtropical. Historical data on rainfall (RF) was extensive and available monthly from 1936 to 2010, from gauging stations in São Luiz do Paraitinga town (23o13' S, 45o19' W) 23 km distant from the sampling site. These data were used to characterize the regional climate. We also measured water temperature (WT) at each sampling in the Ribeirão Grande stream using a regular thermometer. Fish Sampling and Bionomic Data The fishes were collected with six gillnets (mesh sizes from 1.5 to 4.0 cm between adjacent knots), three funnel traps and a seine net (2.0 x 1.0 m, mesh = 0.25 cm between adjacent knots). Sampling effort was standardized to 12 h for gillnets and funnel traps. Seine net effort was standardized as 10 pulls taken during the morning along patches of the submerged macrophyte Potamogeton polygonus Cham. and Schltdl., where the fishes concentrate. Due to water clarity, the seine net was the most efficient method whereas gillnets and funnel traps had low captures. Two other sites were sampled monthly during the same period in Paraibuna and Ipiranga rivers (Gomiero and Braga 2006), but yielded only 3 individuals ofAstyanax intermedius, suggesting that the species does not have self-sustaining populations in those habitats. All procedures were approved by the Animal Ethics Committee at Santa Cecilia University (CEUA - UNISANTA), document CEUA UNISANTA 07/2015. After each sampling, the fishes were fixed in formalin 10% and preserved in alcohol 70%. Voucher specimens were deposited at the fish collection of Universidade Federal do Rio Grande do Sul (UFRGS 10821). In the laboratory we measured the standard length (SL - cm) and total weight (TW - g). Fishes were eviscerated and the sex and the stages of gonadal maturation (GM: immature/juveniles - A, maturing - B, fully mature - C and spent - D) were verified based on size, shape, and degrees of vascularization and transparency (Vazzoler 1996, Alkins-Koo 2000). The stomachs full of food were preserved in alcohol 70% and prey items identified to the lowest feasible taxonomic level using specialized literature (Borror and Delong 1969, Needham and Needham 1982, Costa et al. 2006). To quantify diet, we used the Feeding Preference Degree (FPD) index proposed by Braga (1999) which is a measure of alimentary preference for a group of individuals, and consists of attributing categories to the food items according to their relative abundance in the stomach. When there is only one food item the score of 4 is attributed. When there is more than one item the scores 3, 2 or 1 are adopted, according to the relative predominance of each item. FPD is calculated for each alimentary item through the sum of the attributed scores divided by the number of analyzed stomachs. The food items were subsequently classified as preferential (FPD ≤ 2), secondary (1 ≤ FPD < 2) and occasional (0 < FPD < 1) following Braga (1999). To estimate fecundity all mature and spent ovaries were weighed, immersed in Gilson's solution and periodically shaken to dissociate the oocytes. Once separated, oocytes were preserved in 70% alcohol, and the number of oocytes was estimated according to volumetric method (Vazzoler 1996) by the expression N = S × n/s, whereS is the volume of alcohol used to homogenize the mass of oocytes and n is the total number of oocytes counted in a sample of s volume obtained with a Stempel pipette (2 mL). A subsample of 100 oocytes was randomly selected from each ovary and measured using an ocular micrometric (at 10x magnification). The minimal diameter of mature oocytes was determined by their visual appearance (mature oocytes are opaque, West 1990) considering those greater than or equal to the size of the last mode of the distribution. Batch fecundity was then estimated as Fec = p × N, wherep is the proportion of mature oocytes estimated within each ovary. This procedure, although not as precise as histological determination (West 1990), has been useful to allow fecundity estimation in fractional spawners (Braga et al. 2007, Gomiero et al. 2007, 2008a). The spawning type was verified through the frequency distribution of oocyte sizes. The presence of a single mode of yolky oocytes is characteristic of a total spawner species whereas two or more modes characterize a fractional spawning type (Vazzoler 1996). Individuals with similar frequency distributions were grouped together and the gonadosomatic index (GSI) was calculated for each group according to the expression GSI = (WO/WT) × 100, where WO is the ovarian weight and WT the total fish weight. Analyses of Feeding We calculated the niche breadth (B') using Levins' index (Levins 1968) and the niche overlap (θjk) using Pianka index (Pianka 1973). These indices were calculated for each month to detect monthly changes related to rainfall level or water temperature, and for the size classes in order to detect ontogenetic changes in diversity and composition of alimentary items. The length of the size classes was 5 mm. Niche breadth (B') and niche overlap (θjk) were calculated according to the formula: where Pi is the proportional contribution of food item i in the diet (estimated by the FPD), θjk is the symmetrical niche overlap between months (or size classes) j and k, Pij is the proportion of the resource i used in month (or size classes) j, Pik is the proportion of resource i used in monthk, and n is the total number of food items. A multiple regression model was used to test the dependence of monthly niche breadth on rainfall and water temperature, while a simple linear regression was used to test for changes in niche breadth related to size classes. Niche overlap was modeled as a function of rainfall, water temperature and size classes using Permutational Multivariate ANOVA (Anderson 2001, McArdle and Anderson 2001). The method is based on the partitioning of the sum-of-squares (SS) in a dissimilarity matrix so that a F statistic is calculated as the ratio of among and within groups SS. The analysis was done in R software using function "adonis", which is available in Vegan package (Oksanen et al. 2013). Significance probabilities were calculated from 10000 permutations of the raw data. Analyses of Reproduction Simple ANOVA was used to compare the standard length of immature, males and females. The length of first maturation was determined for males and females with a logistic regression model. The response variable was coded as 0 for immature (GM = A) and 1 for mature individuals (GM = B, C or D). The average length of first maturation was considered the one where 50% of the individuals were mature (L50). RESULTS We collected 765 individuals of Astyanax intermedius and analyzed the food items of 86 full stomachs. The majority of fishes had empty (192) or partially full stomachs (487) and was not analyzed. Diet consisted of 31 food items. Regarding the origin, 13 items were autochthonous, 17 were allochthonous, and one of uncertain origin. Astyanax intermedius consumed mainly animal items such as insects of the Orders Coleoptera (adults), Hymenoptera (Formicidae) and Diptera (mainly Chironomidae larvae). Non-identified fragments of insects (i.e. abdomen, paws, wings, antennae) composed a high proportion of the food. Vegetal items such as algae (Batrachospermum sp. Roth, 1797), flowers, leaves, fruits and seeds occurred occasionally (Table I). TABLE I Food items found in stomachs of Astyanax intermedius and Feeding Preference Degree of each item (FPD) with the respective classification (N = 86 stomachs). Niche breadth were not explained by rainfall (βRF = -0.013, p = 0.421) or water temperature (βWT = -0.007, p = 0.983). The multiple regression model with these two variables explained only 11% of total variation in niche breadth. On the other hand, the linear regression between size classes and niche breadth were highly significant (βWT = 1.07, p = 0.001) explaining 61% of total variation in niche breadth. Niche overlap was also not explained by rainfall (p = 0.941), water temperature (p = 0.144) or size classes (p = 0.996) (Fig. 1). Figure 1 Dispersion diagrams between Levin's niche breadth and monthly rainfall (a), water temperature (oC) (b), and size classes (c). The estimated values for regression parameters in c were: β0 = -0.24, β1 = 1.07, F1,12 = 19.17, p = 0.001, r2 = 0.615 Reproduction The standard length of Astyanax intermedius ranged from 2.0 to 9.0 cm. Females were significantly larger than males and immature (females = 7.24 cm, sd = 1.14cm, males = 5.46 cm, sd = 1.23cm, immature = 2.91 cm, sd = 0.61 cm, F762,2 = 1210, p < 0.001). The mean sizes at first maturation were 4.44 cm for females (95% confidence interval from 3.46 to 5.64) and 3.92 for males (95% confidence interval from 3.26 to 4.74) (Fig. 2). Although the value was higher for females, superposition of the confidence intervals suggest no significant differences. Figure 2 Logistic regression curves for determination of the mean size of first maturation for females (a) and males (b) of Astyanax intermedius in Ribeirão Grande stream. We found mature individuals of A. intermedius throughout the year (Fig. 3) and analyzed 212 pairs of mature ovaries. The mature females ranged from 5.3 to 9.0 cm of standard length. The oocytes diameter ranged from 61.2 to 1,346.4 μm. Mature oocytes were considered those larger than 612 μm (10 d.o.m.). Based on the size frequency distribution of oocytes 3 groups was recognized: i) ovaries with predomination of oocytes in final stages of maturation (N = 42; GSImean = 7.06, sd = 2.05), ii) ovaries with mature oocytes (N = 169; GSImean = 12.58, sd = 5.00), and iii) one ovary recently spent (N = 1; GSI = 5.75) (Fig. 4). The total number of oocytes, which corresponds to those at all stages of development, ranged from 1,125 to 10,350. The potential fecundity, represented by the mature oocytes that are effectively eliminated during spawning season, ranged from 538 to 6,727 (mean = 2,688.7, sd = 1278.8). Figure 3 Percent of individuals of Astyanax intermediusaccording to the stages of gonad maturation (immature/juveniles - A, maturing - B, fully mature - C and spent - D) in Ribeirão Grande stream. Numbers above bars represent the total of individuals captured per month. Figure 4 Frequency distribution (%) of oocytes diameters (µm) inAstyanax intermedius. a - oocytes in early stages of maturation (N = 42 females); b - oocytes in last stages of maturation (N = 169 females) and c - oocyte distribution classes for a female after spawning (N = 1). DISCUSSION In the Atlantic Forest streams of southeast where seasonality is not well marked (no clear delimitation between dry and wet seasons), alimentary resources seem to be homogeneously distributed throughout the year (Gomiero et al. 2007). Although other studies indicate a seasonal variation along the year in the relative importance of autochthonousversus allochthonous items (Winemiller 1990), our results indicate that in Ribeirão Grande stream these food sources are equally important with a little preponderance of allochthonous items (55%), mainly terrestrial insects. Based on alimentary items, Astyanax intermedius was classified as omnivorous and generalists. In such species, an integration between a low alimentary preference with the availability and accessibility of food items that is provided by environment is expected (Angermeier and Karr 1983, Gerking 1994). Other fish species in the region such as Rhamdia quelen (Gomiero et al. 2007), Oligosarcus hepsetus(Botelho et al. 2007) andBrycon opalinus (Gomiero et al. 2008b), also ingested similar fractions of autochthonousand allochthonous items with predominance of Orders Coleoptera, Hymenoptera and Diptera (mainly Chironomidae larvae), suggesting that accessibility and availability play an important role in determining food items consumed. Juvenile stages of Diptera, Ephemeroptera and Odonata are important authochthnous items in diet of stream fishes, while the allochthonous items consist mainly of plant material and terrestrial insects (Luiz et al. 1998). The importance of Diptera, mainly Chironomidae larvae, is high for stream fishes (Henry et al. 1994, Uieda and Gajardo 1996, Castro and Casatti 1997, Uieda et al. 1997, Casatti and Castro 1998, Motta and Uieda 2004, Oliveira and Bennemann 2005). These larvae are aquatic or semi-aquatic, indicating that part of the material exported to the terrestrial environment, after adults emergence, returns to aquatic ecosystem as alimentary source for fishes (Motta 1996, Roque et al. 2003), highlighting the role of aquatic insects for the stream food web structure (Uieda and Pinto 2011). Rainfall and water temperature were not related to niche width or overlap agreeing with the generalist behaviour discussed so far. Nonetheless, the niche width (but not niche overlap) increased with fish size suggesting a diversification (but not a substitution) of food items consumed. This increase is probably related to a better capacity of exploring the available resources (ex. larger mouth and natatory capacity) (Truemper and Lauer 2005, Montaña et al. 2011) and were found for other Characidae species. In Mimagoniates microlepis for example, adults consumed more allochthonous items than juveniles (Barreto and Aranha 2006), and in Deuterodon langei, adults consumed more plant items (Vitule and Aranha 2002). Females were significantly larger than males but size of first maturation were similar. Within the Characidae family it is common to find larger female maturation sizes similar to those found in the present study (Rodrigues et al. 1989, Barbieri and Barbieri 1988, Barbieri 1992a,b, Gurgel 2004, Hojo et al. 2004, Orsi et al. 2004, Mazzoni et al. 2005, Mazzoni and Silva 2006, Abilhoa 2007). In species with promiscuous mating and external fertilization, larger females are expected due to the positive relationship between female size and fecundity. Early maturation of males (Stearns 1992) is also expected, although this reproductive tatic is labile and strongly related to the interaction between genotype and environment (Wootton 1992). These species also invest a large proportion of assimilated resources to reproduction, decreasing the amount available for growth and survivor (Tedesco et al. 2008). Female fecundity varied from 538 to 6,727 oocytes per female and was considered low if compared to its congeners such as A. bimaculatus with fecundity between 1,948 (Gennari Filho and Braga 1996) to 18,040 (Alkins-Koo 2000), or A. janeiroensis from 3,169 to 18,714 oocytes (Mazzoni et al. 2005). Nonetheless, fishes with mature gonad were found throughout the year suggesting an extended reproductive period for A. intermedius. The duration and time of the reproductive period are critical components of life-history strategy, and when environment allows, an extended reproduction with multiple batches of oocytes may increase the probability of recruitment throughout the year. In addition, small fishes tend to have this strategy as a result of space limitation, to produce a unique and large batch of oocytes. In addition, the extended reproductive season may reduce intraspecific competition for food in juveniles, and compensate the larger rates of eggs/larvae mortality due to unpredictable fluctuations in environmental conditions (Nikolskii 1969, Bagenal 1971, Kramer 1978, Matthews 1998, Braga et al. 2007). The patterns of frequency distributions of oocytes diameters also suggested that the spawning was fractional as evidenced by the presence of two modes in the frequency of oocytes diameter distribution and by the gonad somatic index. In small Characidae the fractional spawning is common to other species as A. schubarti(Rodrigues et al. 1995),Bryconamericus iheringii (Lampert et al. 2004) and Moenkhausia intermedia (Braga and Gennari Filho 1991, Hojo et al. 2004) and probably results from adaptive pressures in streams environments (Mazzoni and Silva 2006). All these results suggested that A. intermedius fit as a typical opportunistic strategist (Winemiller and Rose 1992) characterized by a small species, with early maturation, high mortality rates, multiple spawning, small eggs, low fecundity but high reproductive effort, rapid population turnover and capacity for rapid colonisation (Winemiller 1989, Lamouroux et al. 2002, Blanck et al. 2007). This may explain the prevalence of this species in several isolated headwater basins of vegetated Atlantic forested streams where food resources are abundant and distributed throughout the year. ACKNOWLEDGMENTS This project was partially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq process 140180/2005-2) by means of a PhD scholarship provided to the first author, and by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP process 04/12669-3) by providing funding for fieldwork. The Universidade Estadual Paulista (UNESP), Department of Biology, Rio Claro campus provided the institutional support. We thank Dr. Leandro M. Gomiero for providing logistical support during fieldwork and laboratory analyses, Dr. Heraldo A. Britski and Dr. Vinicius A. Bertaco for taxonomic identification and IBAMA (process 02027.000234/2005-05) for issuing the collection license. REFERENCES Abelha MCF, Agostinho AA and Goulart E. 2001. Plasticidade trófica em peixes de água doce. Acta Scietiarum 23: 425-434. Abelha MCF Agostinho AA Goulart E 2001 Plasticidade trófica em peixes de água doce Acta Scietiarum 23 425 434 Abilhoa V. 2007. 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