Identification of caffeic acid and rutin by UHPLC MS/MS and antioxidant activity of <i>Commelina erecta</i> Lineu. in cell culture

OTSUKA, FELIPE A.M.; SANTOS, RODRIGO B.; CHAVES, LARISSA F.; SANTOS, ROSANGELA S.; CHAVES FILHO, ADRIANO B.; MIYAMOTO, SAYURI; MATOS, HUMBERTO R.

doi:10.1590/0001-3765202020190491

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Health Sciences • An. Acad. Bras. Ciênc. 92 (1) • 2020 • https://doi.org/10.1590/0001-3765202020190491 copy

Identification of caffeic acid and rutin by UHPLC MS/MS and antioxidant activity of Commelina erecta Lineu. in cell culture

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The Commelina erecta L. (C. erecta) also known as erva-de-santa-luzia is reported by local population to have medical properties against some pathological conditions. In this study, two extracts of C. erecta leaves (aqueous and ethanolic) were phytochemically analysed and evaluated for their in-vitro antioxidant activities by DPPH, TBARS, NO assays and cell viability assays. The ultra-high performance liquid chromatography followed by tandem mass spectrometry analysis showed the presence of rutin and caffeic acid in aqueous and ethanolic extract. The total polyphenols in aqueous and ethanolic extracts found were 142.7 ± 3.0 and 123.1 ± 5.8 μg/mL of GAE, respectively. The ethanolic extract (5 mg/mL) inhibits TBARS by 33.8%, and the aqueous extract (5 mg/mL) exhibited scavenger property against nitric oxide derivatives to an extent of 77.8%. In cell culture, both extracts improved cell survivability under H₂O₂ induced oxidative stress. Thus, C. erecta extract is a good candidate to become a phytotherapic medicine.

Key words
Antioxidant activity; caffeic acid; Commelina erecta L.; polyphenols

Sample Concentration	Total polyphenols (μg/mL of GAE)		DPPH Inhibition Percent (%)			TBARS (μM)
Sample Concentration	Aqueous	Ethanolic	Aqueous	Ethanolic	BHT	Aqueous	Ethanolic	BHT
1 mg/mL	29.7 ± 0.6	45.4 ± 0.7	0	0	43.0	5.7 ± 0.1	3.5 ± 0.2	3.4 ± 0.1*
2 mg/mL	63.1 ± 2.6	45.4 ± 0.7	0.7 ± 0.2	0.6 ± 0.3	-	5.9 ± 0.1	3.5 ± 0.2	-
3 mg/mL	91.3 ± 14.7	77.8 ± 7.2	1.3 ± 0.4	1.2 ± 0.5	-	5.9 ± 0.1	3.3 ± 0.2	-
4 mg/mL	110.8 ± 2.0	119.7 ± 7.4	2.4 ± 0.8	2.0 ± 0.5	-	5.9 ± 0.25	3.3 ± 0.2	-
5 mg/mL	142.7 ± 3.0	123.1 ± 5.8	5.4 ± 0.7	4.6 ± 0.8	-	5.8 ± 0.1	3.0 ± 0.1	-
Linear coefficient (r)	0.993	0.932	0.935	0.933	-	-	-	-

	Aqueous extract		Ethanolic extract
Extract concentration (mg/mL)	IP	NO_X concentration (µM)	IP	NO_X concentration (µM)
0.5	51.6 ± 5.3	8.7 ± 1.9*	62.8 ± 6.6	6.4 ± 1.7
1	55.9 ± 7.6	7.9 ± 1.5*	74.3 ± 6.9	4.5 ± 1.2
2.5	64.2 ± 3.8	6.4 ± 0.7*	83.4 ± 3.3	2.9 ± 0.6**
5	77.8 ± 2.5	4.0 ± 0.5*	85.6 ± 1.9	2.5 ± 0.5**
Water	-	17.9 ± 0.7	-	-
Ethanol	-	-	52.3 ± 10.7	8.3 ± 2.5

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[1] Correspondence to: Humberto Reis Matos E-mail: hrmatos10@hotmail.com