Microbial inoculation and fertilizer application on growth of cowpea and spore-based assemblages of arbuscular mycorrhizal fungi in its rhizophere

LINO, INGRID A.N.; SILVA, DANIELLE K.A. DA; MARTINS, LINDETE M.V.; MAIA, LEONOR C.; YANO-MELO, ADRIANA M.

doi:10.1590/0001-3765202220201243

Acessibilidade / Reportar erro

Brasil

Anais da Academia Brasileira de Ciências

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

MICROBIOLOGY • An. Acad. Bras. Ciênc. 94 (4) • 2022 • https://doi.org/10.1590/0001-3765202220201243 copy

Microbial inoculation and fertilizer application on growth of cowpea and spore-based assemblages of arbuscular mycorrhizal fungi in its rhizophere

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

In this study, the effect of microbial inoculants and fertilizer application on cowpea (BRS Pujante) growth and on the structure and composition of arbuscular mycorrhizal fungi (AMF) assemblages were evaluated. A completely randomized experiment was set up involving 17 treatments: four with AMF, three with nodulating bacteria, six with AMF + nodulating bacteria, two with phosphorus, one with nitrogen and one control (reference) in five replicates. Plant growth and nutritional content, mycorrhizal colonization, glomerospores number, spore-based AMF assemblages and ecological indices were evaluated. Mycorrhizal inoculants associated with Bradyrhizobium BR3267 strain were more effective than the Microvirga BR3296 strain. Multidimensional scaling analysis showed that Acaulospora longula treatments were more similar among themselves, and distinct from the other treatments. A difference was observed in the structure of AMF community assemblage between treatments with G. albida + Bradyrhizobium BR 3267 and A. longula, with greater Shannon diversity and Pielou equitability indices in the first treatment and greater dominance in the treatment with A. longula only. Long-term studies are required to determine if the successful establishment of A. longula among indigenous species persists over time and if its dominant behavior is not deleterious to the AMF native community.

Key words
Ecology; Fabaceae; mycorrhizae; nodulating bacteria

Treatments	Leaf area (cm²)	P (g kg^-1)	N (g kg^-1)	N (mg plant^-1)
A. longula (AL)	814.63 a	1.73 c	41.24 b	129.37 a
C. etunicatum (CE)	794.65 a	1.97 b	45.76 a	141.3 a
G. albida (GA)	771.29 a	1.70 c	47.24 a	141.74 a
Mix AMF (AL+CE+GA)	765.39 a	1.57 c	41.80 b	140.75 a
Bradyrhizobium BR3267	834.07 a	1.90 b	43.64 a	140.48 a
Microvirga BR3296	731.01 b	1.79 c	41.50 b	131.88 a
Rhizobia Mix (BR3267 + BR3296)	805.98 a	1.76 c	41.78 b	138.86 a
AL longula+BR3267	707.09 b	1.72 c	39.84 b	135.17 a
AL longula+BR3296	668.74 b	2.02 b	43.20 a	146.78 a
CE etunicatum+BR3267	786.12 a	2.02 b	41.42 b	125.08 a
CE etunicaum+BR3296	721.06 b	1.93 b	44.08 a	142.29 a
GA albida+BR3267	776.77 a	1.94 b	43.78 a	131.62 a
GA albida+BR3296	743.66 b	1.84 c	43.48 a	132.86 a
P (50% of the recommended dose)	701.07 b	1.92 b	41.42 b	125.44 a
N (urea)	788.40 a	1.96 b	41.84 b	129.99 a
Control	684.85 b	2.03 b	44.54 a	131.31 a
P (100% of the recommended dose)	868.32 a	2.42 a	44.10 a	144.07 a
CV (%)	11.29	10.81	6.77	10.35

	LA	SD	PH	CL a	CL b	CL total	SDB	RDB	SDB/RDB	P	N	MC	GLOM
LA
SD	0.11^ns
PH	0.20^ns	-0.05^ns
CL a	0.00^ns	-0.05^ns	-0.16^ns
CL b	-0.08^ns	-0.07 ns	-0.18^ns	0.90**
CL total	-0.03^ns	-0.06^ns	-0.16^ns	0.84**	0.92**
SDB	0.24 *	0.20^ns	0.24 *	0.12^ns	0.14^ns	0.13^ns
RDB	0.07^ns	0.10^ns	-0.42 **	0.25 *	0.35 **	0.32 **	0.06^ns
SDB/RDB	0.01^ns	-0.04^ns	0.46 **	-0.15^ns	-0.25 *	-0.23 *	0.26 *	-0.91 **
P	0.14^ns	0.12^ns	0.10^ns	0.04^ns	-0.04^ns	-0.01^ns	-0.11^ns	-0.15 ^ns	0.07^ns
N	0.08^ns	0.16^ns	-0.16^ns	-0.08^ns	-0.17^ns	-0.21 *	-0.16 ^ns	0.03 ^ns	-0.07^ns	0.33 **
MC	0.16^ns	0.03^ns	0.04 ^ns	-0.12^ns	-0.17 ^ns	-0.18^ns	0.00 ^ns	-0.21 ^ns	0.24 *	-0.36 **	0.01 ^ns
GLOM	0.01 ^ns	-0.10 ^ns	-0.03 ^ns	0.04 ^ns	-0.01^ns	0.05 ^ns	0.12 ^ns	0.19 ^ns	-0.09 ^ns	-0.18 ^ns	-0.05^ns	0.06^ns
NOD	0.10 ^ns	0.14^ns	-0.02 ^ns	-0.11^ns	0.00 ^ns	0.03 ^ns	0.02^ns	0.17 ^ns	-0.12 ^ns	0.02^ns	-0.03 ^ns	-0.17^ns	0.10^ns

Taxa of AMF	AL	CE	GA	AMF mix	BR3267	BR3296	Rhizobia Mix	AL + BR3267	AL + BR3296	CE + BR3267	CE + BR3296	GA + BR3267	GA + BR3296	P*	N	Control	P**
Acaulospora elegans	-	-	0.10	-	-	-	-	-	-	-	0.05	-	-	-	-	-	-
Acaulospora longula	11.80	-	0.10	1.52	0.05	-	-	6.71	6.96	-	-	-	-	0.10	-	-	-
Acaulospora mellea	-	-	0.10	-	-	-	0.05	-	-	-	-	-	-	0.10	-	-	-
Acaulospora morrowiae	-	-	0.10	-	-	-	0.05	0.39	-	-	-	-	-	0.24	-	-	-
Acaulospora reducta	0.05	-	0.10	-	-	-	-	-	-	-	-	-	-	0.05	-	-	-
Acaulospora scrobiculata	-	-	0.10	-	-	-	-	-	-	0.05	-	-	-	-	0.05	0.05	0.10
Acaulospora sp1	0.05	-	0.10	-	-	-	-	-	-	-	-	-	-	-	-	-	-
Acaulospora sp2	-	-	0.10	-	-	-	-	-	-	-	-	-	-	-	-	0.05	-
Acaulospora sp3	-	0.05	0.05	0.05	-	-	-	-	-	-	-	-	-	-	-	0.05	-
Acaulospora sp4	-	-	-	0.10	-	-	-	0.10	0.05	-	-	0.06	-	-	-	0.10	-
Acaulospora sp5	-	0.05	-	-	0.05	-	-	0.10	-	0.05	-	-	-	0.05	-	-	-
Acaulospora sp6	-	-	-	0.05	-	0.10	-	0.05	-	0.05	0.05	-	-	-	0.15	-	0.05
Acaulospora sp7	-	-	0.05	0.05	-	-	-	-	-	-	-	-	-	-	-	-	-
Ambispora appendicula	-	0.05	-	-	-	-	0.05	-	-	-	-	-	-	-	-	-	-
Ambispora sp.	-	-	-	-	-	-	-	0.10	-	-	-	-	-	-	-	-	-
Cetraspora pellucida	0.20	-	0.10	0.05	0.10	0.05	0.05	0.25	0.05	0.05	-	0.12	-	0.05	0.05	0.05	0.15
Claroideoglomus etunicatum	0.05	0.44	-	0.05	-	-	-	-	-	0.49	0.54	-	-	-	-	-	-
Corymbiglomus tortuosum	-	-	-	0.05	0.05	-	-	-	-	-	-	-	-	-	-	-	-
Dentiscutata cerradensis	0.05	-	-	-	-	0.05	0.10	-	-	0.20	0.14	0.12	0.05	0.10	0.10	0.05	0.05
Entrophospora infrequens	-	-	-	-	-	-	0.05	-	-	-	-	0.06	-	0.05	-	0.05	-
Gigaspora albida	-	-	0.49	-	-	-	-	-	-	-	-	0.86	1.08	-	-	-	-
Gigaspora gigantea	0.24	0.15	-	0.15	0.10	0.10	0.34	-	0.05	0.05	0.39	-	-	0.10	0.05	0.25	-
Gigaspora margarita	0.05	0.05	-	-	-	-	-	0.15	0.05	-	-	-	-	-	0.05	-	-
Gigaspora sp.	-	0.10	-	0.10	0.05	0.05	-	0.10	0.15	0.25	0.10	-	-	-	0.10	-	-
Glomus sp1	0.34	2.11	1.27	1.81	1.37	1.18	4.12	1.86	1.27	2.55	2.89	2.08	2.11	2.16	3.97	1.37	2.01
Glomus sp2	0.10	0.78	1.57	1.57	1.18	0.49	1.37	2.60	1.03	1.27	1.08	1.22	2.40	1.32	2.01	1.71	1.71
Glomus sp3	-	-	0.05	0.05	-	-	-	-	-	-	-	-	-	-	-	-	-
Racocetra coralloidea	-	-	-	-	0.05	-	0.10	-	-	-	-	-	0.10	0.05	0.05	-	0.05
Septoglomus constrictum	-	0.05	-	0.10	-	-	-	0.10	0.05	0.15	-	0.49	0.05	0.05	-	0.15	0.39
Total species richness	10	10	7	14	9	7	10	12	9	11	8	8	6	13	10	11	8

Inoculation treatment	Shannon	Dominance	Pielou
A. longula (AL)	0.50 b	0.76 a	0.37 b
C. etunicatum (CE)	0.97 ab	0.43 ab	0.72ab
G. albida (GA)	0.86 ab	0.47 ab	0.82 ab
AMF mix (AL+CE+GA)	1.14 ab	0.37 ab	0.78 ab
Bradyrhizobium BR3267	1.03 ab	0.35 ab	0.81 ab
Microvirga BR3296	0.61 ab	0.63 ab	0.78 ab
Rhizobia mix (BR3267 + BR3296)	0.95 ab	0.48 ab	0.63 ab
AL + BR3267	1.02 ab	0.44 ab	0.66 ab
AL + BR3296	0.81 ab	0.56 ab	0.59 ab
CE + BR3267	1.09 ab	0.39 ab	0.76 ab
CE + BR3296	1.15 ab	0.36 ab	0.76 ab
GA + BR3267	1.36 a	0.26 b	0.84 a
GA + BR3296	1.07 ab	0.36 ab	0.82 ab
P* (50%)	1.21 ab	0.30 b	0.81 ab
N (urea)	0.96 ab	0.43 ab	0.78 ab
Control (NI)	1.10 ab	0.39 ab	0.74 ab
P** (100%)	0.86 ab	0.45 ab	0.75 ab

Academia Brasileira de Ciências Rua Anfilófio de Carvalho, 29, 3º andar, 20030-060 Rio de Janeiro RJ Brasil, Tel: +55 21 3907-8100 - Rio de Janeiro - RJ - Brazil
E-mail: aabc@abc.org.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Correspondence to: Adriana M. Yano-Melo E-mail: amyanomelo@gmail.com