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CHEMICAL SCIENCES • An. Acad. Bras. Ciênc. 95 (suppl 1) • 2023 • https://doi.org/10.1590/0001-3765202320220624 linkcopy

Open-access The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles

AuthorshipSCIMAGO INSTITUTIONS RANKINGS

    Abstract

    SARS-CoV-2 is a matter of concern. Here, biosensors were prepared using iron magnetic nanoparticles containing antibodies against the receptor binding domain (RBD) of the spike protein. Antibodies were adsorbed to nanoparticles in three configurations, including direct adsorption without functionalization (DANPs). Nanoparticles were added to a glassy carbon electrode and connected to an electrochemical cell. Electrochemical impedance spectroscopy and ELISA experiments indicated that antibodies were desorbed from the DANPs upon the addition of the RBD. DANPs-based biosensors produced linear curves with decreasing charge transfer resistance due to the removal of antibodies. Thus, a detection method can be based on antibody desorption.

    Key words
    Biosensor; electrochemical impedance spectroscopy; spike protein; receptor binding domain; COVID-19; SARS-CoV-2

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