In vitro selection and characterization of probiotic properties in eight lactobacillus strains isolated from cocoa fermentation

ALMEIDA, MILENA E. DE; PESSOA, WALLACE FELIPE B.; MELGAÇO, ANA CLARA C.; RAMOS, LOUISE P.; REZENDE, RACHEL P.; ROMANO, CARLA CRISTINA

doi:10.1590/0001-3765202220220013

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MICROBIOLOGY • An. Acad. Bras. Ciênc. 94 (suppl 4) • 2022 • https://doi.org/10.1590/0001-3765202220220013 linkcopy

In vitro selection and characterization of probiotic properties in eight lactobacillus strains isolated from cocoa fermentation

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Traditionally, probiotic microorganisms are isolated from human and animal intestinal microbiota. However, the demand for diversification of biofunctional products has driven the search for new sources of probiotic candidates, such as fermented foods and vegetables. The present study found that strains isolated from the fermentation of fine cocoa from southern Bahia have biotechnological potential for use as a probiotic, since they showed capacity for self-aggregation and co-aggregation, antimicrobial activity against intestinal pathogens and resistance to gastrointestinal transits. Scores of importance for each property were established in order to more accurately assess the probiotic potential of the strains. The tests carried out contemplate the criteria previously established for the selection of probiotic candidates.

Key words
cocoa; lactic acid bacteria; technological properties; lactobacillus; probiotics

SELF - AGGREGATION	0.10
CO - AGGREGATION	0.10
HYDROPHOBICITY	0.20
ANTIMICROBIAL ACTIVITY	0.25
pH	0.20
BILE	0.15

Group	Antimicrobial					Susceptibility
Group	Name	Disk Conc. (µg)	L.P 1.1	L.P 2.1	L.P 2.2	L.P A1	L.P A2	L.F A2	L.F A5	L.F 3.2
Cell wall synthesis inhibitors
Penicillins	Amoxicillin	10	S	S	S	S	S	S	S	S
	Ampicillin	10	S	S	S	S	S	S	S	S
	Penicillin G	10	S	S	MS	S	S	S	S	S
Cephalosporins	Cephalothin	30	S	S	S	S	R	S	S	S
Glycopeptides	Vancomycin	30	R	R	R	R	R	R	R	R
Protein synthesis inhibitors
Aminoglycosides	Gentamicin	10	R	R	R	R	R	R	R	R
	Streptomycin	10	R	R	R	R	R	R	R	R
Tetracyclines	Tetracycline	30	S	S	S	S	S	MS	S	S
Aminoglycosides	Gentamicin	30	S	S	S	R	S	S	S	S
Macrolides	Erythromycin	15	S	S	S	S	S	S	S	S
Lincosamides	Clindamycin	2	S	R	S	S	S	S	S	S
Nucleic acid synthesis inhibitors
Quinolones	Ciprofloxacin	5	R	R	R	R	R	R	R	R
	Norfloxacin	10	R	R	R	R	R	R	R	R

Strains	Moderate and high hydrophobicity	Self- aggregation	Co-aggregation E. coli	Antimicrobial activity	Co-aggregation S. Enteritidis	Antimicrobial activity	Partial score
L. plantarum 2.1	12.12	2.42	1.37	2	1.56	2	21.47
L. plantarum 2.2	9.70	2.27	2.12	0	0.89	0	14.98
L. plantarum A1	11.72	2.36	0	1.5	0	2	17.58
L. plantarum A2	16.56	2.22	1.43	2	0.13	1.5	23.84
L. fermentum A2	13.42	2.25	1.14	0	0	0	16.81

Strains	pH resistance (%)	Score	Bile Resistance (%)	Score	Final score
L. plantarum 2.1	45	9	4.8	0.72	31.19
L. plantarum A2	57	11.4	0.7	0.105	35.34

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