Open-access Propagation in vitro of Baptistonia pubes (Lindl.) Chiron & V.P. Castro (Oncidium pubes Lindl. - Orchidaceae)

Baptistonia pubes is an epiphyte that grows in Brazilian Atlantic Forest, in Paraguay and northern Argentina. It is considered vulnerable and threatened with extinction. Our aim was to verify seed germination efficiency and growth at two pHs, using two culture mediums: Murashige-Skoog (modified - (MS)) and banana pulp with added NPK 20-20-20 (Plant Prood®) (BAN), with varied nutrient and pH concentrations for germination and for growth. The seeds germinated on average 15 days after the inoculation, at all conditions. The percentage of germination varied between 2.6 and 11.6 %. The highest percentage was at pH=5.2 BAN and the lowest at pH=6.2 MS/2. The banana pulp was most efficient for germination. The pHs, in each environment, altered significantly the averages of the germinated seeds. Seedling growth showed better results in the banana medium, pH = 5.2

Germination; growth; pH variation; culture medium


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