With the aim of verifying the occurence of arbuscular mycorrhizal fungi AMF, Glomales, Zygomycota), rhizosphere soil samples were collected in three areas (eucaliptus reforestation, forest and intersection) and on three occasions (March/1996, rainy and warm; June/1996, dry and cold; and January/1997, the beginning of both the rainy season and the summer) on the "Ilha dos Eucaliptos" (ca. 323.500m²) in the Guarapiranga Reservoir, São Paulo, SP, Brazil. Soil samples were processed by the wet sieving technique, followed by sucrose centrifugation (50% V:V ). The isolated spores were prepared in vouchers, with polivynil alchool lactofenol resin (PVL). We observed 21 taxa of AMF: Acaulospora excavata Ingleby, Walker & Mason, Acaulospora foveata Trappe & Janos, Acaulospora longula Spain & Schenck, Acaulospora mellea Spain & Schenck, Acaulospora morrowiae Spain & Schenck, Acaulospora spinosa Walker & Trappe, Acaulospora sp. 1, Acaulospora sp. 2, Acaulospora sp. 3, Acaulospora sp. 4, Entropliospora colombiana Spain & Schenck, Gigaspora gigantea Nicol. & Gerd., Gigaspora ramisporophora Spain, Sieverding & Schenck, Glomus aggregatum (Schenck & Smith) Koske, Glomus etunicatum Becker & Gerd., Glomus geosporum (Nicol. & Gerd.) Walker, Glomus invermayum Hall, Glomus macrocarpum Tul. & Tul., Glomus multisubstensum Mukerjii, Bhattacharjee & Tewari, Scutellospora aff. biornata Spain, Sieverding & Toro, and Scutellospora erythropa Koske & Walker. The Sõrensen Index showed 64% to 90% of similarity between the areas and the collectings, this suggesting the absence of influence of these kinds of parameters on the mycota.
AMF; diversity; similarity