Influence of exogenous abscisic acid on physiological responses of Dendrobium candidum under drought stress

Tian, Feng-xia; Xu, Guang-ling; Wang, Cai-xia; Wei, Yu-qing; Zhou, Suo

doi:10.1590/1677-941X-ABB-2023-0217

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Original Article • Acta Bot. Bras. 39 • 2025 • https://doi.org/10.1590/1677-941X-ABB-2023-0217 linkcopy

Influence of exogenous abscisic acid on physiological responses of Dendrobium candidum under drought stress

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abscisic acid (ABA) is a naturally occurring plant hormone, and it is also a known stress hormone that acts in plant responses to abiotic stresses, especially drought. Dendrobium candidum production losses due to drought have prominent economic effects. The current study investigates the role of ABA in the drought stress tolerance of D. candidum. The influence of drought stress and foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) on physiological and biochemical parameters was analyzed. The stems and leaves of D. candidum were collected on Day 0, Day 6, and Day 12. The osmotic regulation ability, photosynthetic maintenance ability, and antioxidant ability of D. candidum under drought stress were measured. Additionally, the changes in growth and physiological characteristics of D. candidum under drought stress were studied after applying three concentrations of ABA. Exogenous ABA application effectively ameliorates the adverse effects of drought stress to improve drought resistance. In conclusion, 10 mg/l ABA resulted in leaf water status maintenance, photosynthetic performance, and antioxidative ability under drought stress.

Keywords:
ABA; antioxidant ability; Dendrobium candidum; drought stress; drought resistance

Authorship

person Feng-xia Tian *

conceptualization · visualization · supervision · writing original draft

0000-0001-9107-6251

person Guang-ling Xu

conceptualization · visualization

schoolNanyang Normal University, College of Life Sciences, Nanyang, Henan 473061, ChinaNanyang Normal UniversityChinaNanyang, Henan, ChinaNanyang Normal University, College of Life Sciences, Nanyang, Henan 473061, China

0009-0000-6990-0486

person Cai-xia Wang

writing review & editing

0009-0009-6906-8242

person Yu-qing Wei

writing review & editing

0009-0001-0696-6573

person Suo Zhou

conceptualization · visualization

0009-0008-6898-1438

person*Corresponding Author: Fengxia Tian:
emailtianwenxiu1984@163.com

Editor Chef:

Thais Almeida

Associate Editor:

Marina Scalon

Conflict of Interest

The author(s) declare that they have no competing interests.

SCIMAGO INSTITUTIONS RANKINGS

Nanyang Normal University, College of Life Sciences, Nanyang, Henan 473061, ChinaNanyang Normal UniversityChinaNanyang, Henan, ChinaNanyang Normal University, College of Life Sciences, Nanyang, Henan 473061, China

Figures

Figures (7)

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Fig. 1 Changes in (a) water potential, (b) relative water content in leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance.

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Fig. 2 Changes in (a) soluble protein, (b) soluble sugar, and (c) free proline contents in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance.

Thumbnail

Fig. 3 The (a) chlorophyll content (a), (b) ΦPSII, and (c) Fv/Fm in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance.

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Fig. 4 (a) Net photosynthetic rate (A), (b) intercellular CO₂ concentration (Ci), (c) transpiration rate (E, mmol H₂O/m² s), and (d) stomatal conductance (gs) in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance.

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Fig. 5 Changes in (a) malondialdehyde content and (b) relative electrical conductivity in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance.

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Fig. 6 Changes in (a) O₂ ^•¯ accumulation and H₂O₂ accumulation, (b) O₂ ^•¯ production rate, and (c) H₂O₂ contents in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates, and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance.

Thumbnail

Fig. 7 Activities of (a) SOD, (b) POD, (c) CAT, and (d) APX in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance.

imageFig. 1 Changes in (a) water potential, (b) relative water content in leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. open_in_new

imageFig. 2 Changes in (a) soluble protein, (b) soluble sugar, and (c) free proline contents in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. open_in_new

imageFig. 3 The (a) chlorophyll content (a), (b) ΦPSII, and (c) Fv/Fm in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. open_in_new

imageFig. 4 (a) Net photosynthetic rate (A), (b) intercellular CO₂ concentration (Ci), (c) transpiration rate (E, mmol H₂O/m² s), and (d) stomatal conductance (gs) in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. open_in_new

imageFig. 5 Changes in (a) malondialdehyde content and (b) relative electrical conductivity in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. open_in_new

imageFig. 6 Changes in (a) O₂ ^•¯ accumulation and H₂O₂ accumulation, (b) O₂ ^•¯ production rate, and (c) H₂O₂ contents in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates, and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. open_in_new

imageFig. 7 Activities of (a) SOD, (b) POD, (c) CAT, and (d) APX in the leaves of Dendrobium candidum seedlings under the control treatment (CK) and drought stress (DS) after foliar spray with ABA at different concentrations (5 mg/l, 10 mg/l, 15 mg/l) for 0, 6 and 12 days. Data is presented as the mean ± SE of three replicates and statistical significance was determined by the Tukey test (P < 0.05). Note: Capital letters indicate inter-group significance and lowercase letters indicate intra-group significance. open_in_new

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