A study of the association of glutathione S-transferase M1/T1 polymorphisms with susceptibility to vitiligo in Egyptian patients

Aly, Dalia Gamal; Salem, Samar Abdallah; Amr, Khalda Sayed; El-Hamid, Mahmoud Fawzy Abd

doi:10.1590/abd1806-4841.20185796

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Investigation • An. Bras. Dermatol. 93 (1) • Jan-Feb 2018 • https://doi.org/10.1590/abd1806-4841.20185796 copy

A study of the association of glutathione S-transferase M1/T1 polymorphisms with susceptibility to vitiligo in Egyptian patients^* * Work performed at the Department of Dermatology and Venereology, National Research Centre - Department of Medical Molecular Genetics, National Research Centre, Giza, Egypt. Department of Dermatology, Venereology and Andrology, Ain Shams University, Abbasseya Square - Cairo, Egypt.

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract:

Background:

The association of glutathione S-transferases M1/T1 (GSTM1/T1) null polymorphisms with vitiligo was proposed in several studies including two Egyptian studies with contradictory results.

Objective:

The aim here was to assess the association between GSTM1/T1 null polymorphisms and the susceptibility to vitiligo in a larger sample of Egyptian patients with generalized vitiligo.

Methods:

This study included 122 vitiligo patients and 200 healthy controls that were age, and gender matched. Assessment of GSTM1/T1 gene polymorphisms was done using a multiplex polymerase chain reaction (PCR).

Results:

Increased odds of generalized vitiligo was observed with the null genotypes of GSTM1 and GSTT1 polymorphisms (P<0.05). Controls with GSTM1 null/GSTT1+ heterozygosis presented with a 2.97 odds protection from having generalized vitiligo (OR=2.97, 95%CI=1.1-7.7) (P=0.02) compared with patients.

Study Limitations:

Small sample size of patients.

Conclusions:

This study showed a significant trend towards an association with the combination of the GSTM1/GSTT1 double null polymorphism and generalized vitiligo. Individuals with GSTM1 null/GSTT1+ heterozygosis have a 2.97 odds protection from having generalized vitiligo compared with patients. It was is the first time, to our knowledge, that such an association has been reported.

Keywords:
Glutathione S-Transferase pi; Oxidative stress; Polymorphism, single nucleotide; Vitiligo

Genotype		Group				OR (95% CI)	P-value
		Control(n=200)		Generalized(n=122)
		N	%	N	%
GSTM1	Present	89	44.5	58	47.5	0.885(0.56-1.3)	0.59c
GSTM1	Null	111	55.5	64	52.5	0.885(0.56-1.3)	0.59c
GSTT1	Present	118	59.0	59	48.4	1.5(0.97-2.4)	0.06c
GSTT1	Null	82	41.0	63	51.6	1.5(0.97-2.4)	0.06c
NULL	Positive	8	4.0	12	9.8	0.382(0.15-0.96)	0.03c^* * Significant
NULL	Negative	192	96.0	110	90.2	0.382(0.15-0.96)	0.03c^* * Significant
GSTM1/GSTT1	+/ +	15	7.5	7	5.7	3.2(0.90-11.4)	0.07L
	-/ +	103	51.5	52	42.6	2.97(1.1-7.7)	0.02L^* * Significant
	+/ -	74	37.0	51	41.8	2.17(0.83-5.7)	0.1L
	Double Null	8	4.0%	12	9.8	1

		GSTM1			GSTT1			Null
		Present	Null	P- value	Present	Null	P-value	Present	Null	P- value
Sex	Male n(%)	17 (29.3%)	16 (25%)	0.592c	16 (27.1%)	17 (27%)	0.987c	4 (33.3%)	29 (26.4%)	0.733c
Sex	Female n(%)	41 (70.7%)	48 (75%)	0.592c	43 (72.9%)	46 (73%)	0.987c	8 (66.7%)	81 (73.6%)	0.733c
Activity	Stable n(%)	21 (36.2%)	24 (37.5%)	0.882c	22 (37.3%)	23 (36.5%)	0.929c	5 (41.7%)	40 (36.4%)	0.758F
Activity	Active n(%)	37 (63.8%)	40 (62.5%)	0.882c	37 (62.7%)	40 (63.5%)	0.929c	7 (58.3%)	70 (63.6%)	0.758F
Family History	Negative n(%)	42 (72.4%)	49 (76.6%)	0.599c	44 (74.6%)	47 (74.6%)	0.997c	11 (91.7%)	80 (72.7%)	0.292F
Family History	Positive n(%)	16 (27.6%)	15 (23.4%)	0.599c	15 (25.4%)	16 (25.4%)	0.997c	1 (8.3%)	30 (27.3%)	0.292F
Age (Mean ± SD)		30.28 ±12.77	29.38 ±12.77	0.698t	30.13 ± 13.1	29.56 ± 12.43	0.806 t	29.23 ± 12.1	35.58 ± 17.1	0.101 t
Duration (Mean ± SD)		2.83 ± 1.68	2.73 ± 1.94	0.775 t	2.45 ± 1.52	3.14 ± 2.01	0.036 t	2.84 ± 1.84	2.25 ± 1.29	0.281 t

		GSTM1+/GSTT1-	GSTM1-/GSTT1+	GSTM1+/GSTT1+	GSTM1-/GSTT1-	P- value
Sex	Male n(%)	13 (25.5%)	12 (23.1%)	4 (57.1%)	4 (33.3%)	0.255F
Sex	Female n(%)	38 (74.5%)	40 (76.9%)	3 (42.9%)	8 (66.7%)
Activity	Stable n(%)	18 (35.3%)	19 (36.5%)	3 (42.9%)	5 (41.7%)	0.944 F
Activity	Active n(%)	33 (64.7%)	33 (63.5%)	4 (57.1%)	7 (58.3%)
Family History	Negative	36 (70.6%)	38 (73.1%)	6 (85.7%)	11 (91.7%)	0.5 F
Family History	Positive	15 (29.4%)	14 (26.9%)	1 (14.3%)	1 (8.3%)
Age (Mean ± SD)		28.84 ± 11.82	29.06 ± 11.42	33.29 ± 19.15	35.58 ± 17.1	0.328A
Duration (Mean ± SD)		2.5 ± 1.58	2.96 ± 1.74	4.43 ± 3.36	2.25 ± 1.29	0.21 A

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[1] Conflict of interest: None.