[Mastocitoma cutâneo em um cachorro-do-mato vinagre (Speothos venaticus) de cativeiro: aspectos patológicos e imunofenotípicos - relato de caso]

Um cachorro-vinagre (Speothos venaticus) apresentou um nódulo cutâneo pequeno na região perianal. Histologicamente havia neoplasia cutânea de células redondas e ulcerada, constituída por mastócitos bem diferenciados, com abundantes grânulos citoplasmáticos metacromáticos na coloração de Giemsa e infiltrado eosinofílico difuso. A imuno-histoquímica demonstrou que 30% das células neoplásicas eram positivas para a proteína Kit no citoplasma e na membrana celular. As células foram negativas para MAC e CD3. Menos de 10% das células neoplásicas foram positivas para Ki67. Durante a necropsia, foram identificados outros tumores primários, como adenoma intestinal, adenoma cortical da adrenal e tumor de células intersticiais do testículo.

Palavras-chave:
canídeos selvagens; imuno-histoquímica; tumores múltiplos

Autoria

D.O. Santos

Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, BrasilUniversidade Federal de Minas GeraisBrazilBelo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0002-3083-1572

A.R. Oliveira

https://orcid.org/0000-0002-9031-792X

T.P. Carvalho

https://orcid.org/0000-0002-7158-8249

N.F. Paula

https://orcid.org/0000-0002-2506-1163

H.P. Tinoco

Fundação de Parques Municipais e Zoobotânica, Jardim Zoológico, Belo Horizonte, MG, BrasilFundação de Parques Municipais e ZoobotânicaBrasilBelo Horizonte, MG, BrasilFundação de Parques Municipais e Zoobotânica, Jardim Zoológico, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0003-4270-5503

C.M. Coelho

https://orcid.org/0000-0002-5689-6198

A.T.P.G. Ribeiro

https://orcid.org/0000-0003-0339-1767

G.C. Martins

Centrovet, Belo Horizonte, MG, BrasilCentrovetBrasilBelo Horizonte, MG, BrasilCentrovet, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0002-4317-4233

E.S. Veloso

Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Belo Horizonte, MG, BrasilUniversidade Federal de Minas GeraisBrazilBelo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0003-4696-7738

E. Ferreira

https://orcid.org/0000-0002-1835-0303

F. Pierezan

https://orcid.org/0000-0002-3319-8784

R.L. Santos ^**Corresponding author: rsantos@vet.ufmg.br

https://orcid.org/0000-0002-4830-0470

*Corresponding author: rsantos@vet.ufmg.br

SCIMAGO INSTITUTIONS RANKINGS

Centrovet, Belo Horizonte, MG, BrasilCentrovetBrasilBelo Horizonte, MG, BrasilCentrovet, Belo Horizonte, MG, Brasil

Figuras | Tabelas

Figuras (3)
Tabelas (2)

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Figure 1
Cutaneous mast cell tumor in a bush dog (Speothos venaticus). a. Ulcerated, infiltrative, poorly demarcated, and non-encapsulated round cell neoplasm confined to superficial dermis, HE, 50x. b. Well differentiated neoplastic mast cells with mild nuclear and cellular pleomorphism and mitotic figures (arrows). Marked infiltration of eosinophils. HE, 400x. c. Moderate number of purple granules at the cytoplasm of the neoplastic cell, characterizing the mast cell origin, Giemsa, 400x.

Thumbnail

Figure 2
Immunohistochemistry of a cutaneous mast cell tumor in a bush dog (Speothos venaticus). a. Mild immunolabeling of KIT protein, observed both at cell membrane and cytoplasm, from > 30% neoplastic cells, DAB, 200x. b. Proliferation index using Ki67 with nuclear immunolabeling in < 10% of neoplastic cells, DAB, 200x. c. Higher magnification of a, DAB, 1000x. d. Higher magnification of b, DAB, 1000x.

Thumbnail

Figure 3
Intestinal adenoma, adrenal cortex adenoma and testicular interstitial cell tumor in a bush dog (Speothos venaticus). a,b. Intestinal adenoma. a. Intestinal mucosa with a polypoid proliferation projecting into the lumen. HE, 50x. b. Well differentiated epithelial cells organized in tubules with abundant goblet cells. HE, 100x. c,d. Adrenal cortex adenoma. c. Cortical expansive neoplasm organized in nests separated by connective tissue and with multifocal areas of mineralization. HE, 50x. d. Well differentiated neoplastic cells with minimal cellular and nuclear pleomorphism and vacuolated cytoplasm. HE, 200x. e. Testicular interstitial cell tumor. Well differentiated interstitial neoplastic cells with vacuolated cytoplasm between seminiferous tubules. HE, 200x.

Thumbnail

Table 1
Immunohistochemistry protocol for detection of MAC, CD3, c-KIT, and Ki67*

Thumbnail

Table 2
Immunohistochemistry (IHC) results for MAC, CD3, KIT protein and Ki67 expression in the cutaneous mast cell tumor neoplastic cells in a bush dog (Speothos venaticus)

Figure 1 Cutaneous mast cell tumor in a bush dog (Speothos venaticus). a. Ulcerated, infiltrative, poorly demarcated, and non-encapsulated round cell neoplasm confined to superficial dermis, HE, 50x. b. Well differentiated neoplastic mast cells with mild nuclear and cellular pleomorphism and mitotic figures (arrows). Marked infiltration of eosinophils. HE, 400x. c. Moderate number of purple granules at the cytoplasm of the neoplastic cell, characterizing the mast cell origin, Giemsa, 400x.

Figure 2 Immunohistochemistry of a cutaneous mast cell tumor in a bush dog (Speothos venaticus). a. Mild immunolabeling of KIT protein, observed both at cell membrane and cytoplasm, from > 30% neoplastic cells, DAB, 200x. b. Proliferation index using Ki67 with nuclear immunolabeling in < 10% of neoplastic cells, DAB, 200x. c. Higher magnification of a, DAB, 1000x. d. Higher magnification of b, DAB, 1000x.

Figure 3 Intestinal adenoma, adrenal cortex adenoma and testicular interstitial cell tumor in a bush dog (Speothos venaticus). a,b. Intestinal adenoma. a. Intestinal mucosa with a polypoid proliferation projecting into the lumen. HE, 50x. b. Well differentiated epithelial cells organized in tubules with abundant goblet cells. HE, 100x. c,d. Adrenal cortex adenoma. c. Cortical expansive neoplasm organized in nests separated by connective tissue and with multifocal areas of mineralization. HE, 50x. d. Well differentiated neoplastic cells with minimal cellular and nuclear pleomorphism and vacuolated cytoplasm. HE, 200x. e. Testicular interstitial cell tumor. Well differentiated interstitial neoplastic cells with vacuolated cytoplasm between seminiferous tubules. HE, 200x.

Table 1 Immunohistochemistry protocol for detection of MAC, CD3, c-KIT, and Ki67*

Antibody	Manufacturer	Clone	Dilution	Incubation time (h)	Antigen retrieval**	Amplification
MAC387	AbdSerotec	MCA874G	1:400	16	Citratebuffer+Pascal	Novolink
CD3	Dako	Polyclonal	1:300	16	Citratebuffer+Pascal	Novolink
c-KIT	Dako	Polyclonal	1:800	16	Citratebuffer+Pascal	Novolink
Ki-67	Dako	MIB-1	1:50	16	Citratebuffer+Pascal	Novolink

*MAC: anti-macrophage; CD3: anti-T lymphocyte; c-KIT: anti-KIT; Ki-67: proliferation cell marker.
** Pascal - Pressurized humid heat (125(C).

Table 2 Immunohistochemistry (IHC) results for MAC, CD3, KIT protein and Ki67 expression in the cutaneous mast cell tumor neoplastic cells in a bush dog (Speothos venaticus)

IHC antibody	Results	Localization
MAC	Negative	-
CD3	Negative	-
KIT protein	> 30%	Membrane and cytoplasm
Ki67	< 10%	Nuclei

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Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

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[1] *Corresponding author: rsantos@vet.ufmg.br