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Preservation of bovine preantral follicles in 0.9% saline solution or TCM 199

The efficiency of 0.9% saline solution (SS) and TCM 199 on the preservation of bovine preantral follicles (PF) in situ at different temperatures and incubation times was investigated. Each ovarian pair was divided into 25 fragments. One fragment was taken randomly and immediately fixed (control). The other fragments were distributed in tubes containing SS or TCM 199 at 4, 20 or 39ºC for 2, 4, 12 or 24h. The histological analysis showed that the storage at 4ºC in both solutions kept the percentage of normal follicles similar to control values. Preservation in SS at 20ºC for 12 or 24h, TCM 199 at 20ºC for 24h and in both solutions at 39ºC from 2 h onward (P<0.05) increased the percentage of degenerated follicles when compared with control. In both solutions, independent of incubation time, the percentage of normal follicles observed at 39ºC was (P<0.05) lower them those observed at 4 and 20ºC. Bovine PF can be preserved efficiently at 4ºC for up to 24h in both solutions, at 20ºC for 4 and 12h in SS and TCM 199, respectively.

bovine; preantral follicle; preservation


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