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Increase of choroidal and scleral MCP-1 expression in hypercholesterolemia experimental model

Rogil José de Almeida Torres Lucia de Noronha Antonio Marcelo Barbante Casella Thaís Isabel Lumikoski Leonardo Brandão Précoma Caroline Luzia de Almeida Torres Andréa Luchini Mario Claudio Soares Sturzeneker Dalton Bertolim Précoma About the authors

PURPOSE: The aim of this study is to experientially demonstrate that a cholesterol-enriched diet induces an increase in the MCP-1 expression in the choroid and sclera. METHOD: New Zealand rabbits were divided into two groups: GN (normal diet group) of 8 rabbits (8 eyes) was fed a standard rabbit diet for 4 weeks; GH (hypercholesterolemic group) of 13 rabbits (13 eyes) was fed a 1% cholesterol enriched diet for 8 weeks. Total serum cholesterol, triglyceride, HDL cholesterol and fasting blood glucose exams were performed at the start of the experiment and at the euthanasia time. After GH 8th week and GN 4th week animals were euthanized and their eyes underwent immunohistochemical analysis with the anti-MCP-1 antibody. RESULTS: The diet has induced a significant increase in GH total cholesterol and triglyceride levels when compared with NG (p<0.001). There was a significant increase in the MCP-1 expression in GH choroid and sclera in relation to GN (p<0.001). CONCLUSION: This study has revealed that the hypercholesterolemic diet in rabbits induces an increase in the MCP-1 expression in the choroid and sclera.

Macrophage activation; Macular degeneration; Atherosclerosis; Cholesterol; Choroid; sclera; Monocyte chemoattractant proteins; Animals; Rabbits

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