The effects of enoxaparin on the liver in experimental pneumoperitoneum model

Thirty four rats were divided into three groups: Group 1 (sham) underwent insertion of Veress needle into the abdomen and 90 min of anesthesia with no gas insufflation. The animals in control and enoxaparin groups were subjected to 90 min of 14 mmHg CO₂ pneumoperitoneum. Enoxaparin (100 u/kg) was administered subcutaneously to the rats in enoxaparin group one hour before the operation. After 90 min of pneumoperitoneum, the rats were allowed for reperfusion through 60 min. Blood and liver samples were obtained for biochemical and histopathological examination.

RESULTS:

Treatment with enoxaparin decreased the histopathological abnormalities when compared with the control group. The highest levels of oxidative stress parameters were found in control group. The use of enoxaparin decreased the levels of all oxidative stress parameters, but the difference between the control and enoxaparin groups was not statistically significant.

CONCLUSION:

Enoxaparin ameliorated the harmful effects of high pressure CO₂ pneumoperitoneum on the liver.

Key words:
Laparoscopy; Pneumoperitoneum; Artificial; Carbon Dioxide; Enoxaparin; Liver; Oxidative Stress; Rats

GROUPS	CONGESTION	NECROSIS	VACUOLIZATION
SHAM	0.25±0.12	0.00±0.00	0.00±0.00
CONTROL	2.75±0.45^a,c	1.83±0.71^a,c	0.00±0.00
ENOXAPARIN	1.64±0.49^b	0.42±0.19	0.00±0.00

GROUPS	AST	ALT	ALP
SHAM	103.37±23.14	57.37±12.24	193.42±60.55
CONTROL	210.08±99.59^a	97.92±44.21^a	251.28±99.28
ENOXAPARIN	165.57±77.83^b	73.00±15.49^b	223.37±56.71

GROUPS	MDA	AOPP	DFOA
SHAM	39.82±14.33	871.67±229.43	97.61±17.21
CONTROL	73.40±42.07^a	1472.55±388.09^a	142.97±31.10^a
ENOXAPARIN	64.53±26.09^b	1343.73±313.68^b	134.06±39.02^b

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

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[1] Correspondence: Turgut Cavusoglu SB Ankara Egitim ve Arastirma Hastanesi Genel Cerrahi Klinigi, 06340, Ulucanlar Ankara, Turkey Phone: +90.312.595 3424 turgutcavusoglu@gmail.com

Grade	Sinusoidal Congestion	Vacuolization	Necrosis
0	No	No	No
1	Mild	Mild	Simple cell necrosis
2	Moderate	Moderate	0-30%
3	Severe	Severe	30-60%
4	Very severe	Very severe	More than 60%

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The effects of enoxaparin on the liver in experimental pneumoperitoneum model1 1 Research performed at Department of General Surgery, Ankara Education and Research Hospital, Turkey.

ABSTRACT

PURPOSE:

METHODS:

RESULTS:

CONCLUSION:

The effects of enoxaparin on the liver in experimental pneumoperitoneum model^¹ 1 Research performed at Department of General Surgery, Ankara Education and Research Hospital, Turkey.