Acessibilidade / Reportar erro

Brasil

Acta Cirúrgica Brasileira

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Original Articles • Acta Cir. Bras. 29 (9) • Sept 2014 • https://doi.org/10.1590/S0102-8650201400150003 copy

Collared Pecary (tayassu tajacu) as a new model of renal ischemic injury induced by clamping the renal artery^I 1 Research performed at Preclinical Studies Center (CEPREC), Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Department of Veterinary Morphophysiology, Federal University of Piaui (UFPI), Teresina-PI, Brazil. Part of Master degree thesis, Postgraduate Program in Animal Science, UFPI. Tutor: Maria Acelina Martins de Carvalho.

Authorship SCIMAGO INSTITUTIONS RANKINGS

PURPOSE:

The use of the collared peccary as an experimental model for ischemic nephropathy.

METHODS:

A total of 12 collared peccary (Tayassu tajacu) was used and ischemic nephropathy was induced in six of these animals that constituted the experimental group (G1) while the other six formed the control group (G2). Ischemic nephropathy was induced surgically by partial occlusion of the left renal artery. The disease course was assessed by hematological tests, serum chemistry, urinalysis, ultrasound (US) and doppler ultrasound function of the renal artery before induction, and at five, 10, 15 and 20 days after surgery. Twenty days after the occlusion, unilateral nephrectomy and histopathological examination were performed to assess renal morphology.

RESULTS:

Statistical analysis by Fischer's test showed a significant difference (p<0.05) between the control group and the experimental group. The histopathological examination showed glomerular, tubular and interstitial lesions. In the experimental group, 83.3% (5 /6) showed moderate renal lesions and only 16.7% (1/6) were classified with no lesions. The ultrasound examination of the right kidney presented statistical difference between day 5 and day 10 post occlusion.

CONCLUSION:

The collared peccary as a good experimental model for ischemic renal disease, because it could be manipulated during the research time without death, with health conditions that permit any subsequent procedure for disease therapy.

Artiodactyla; Kidney Diseases; Ischemia; Models, Animal

Evaluated data	0 day	5 days	10 days	15 days	20 days
Urea (mg/dL)	24.20±4.55^a	23.48±2.40 ^a	26.86±3.36 ^a	37.66±13.49 ^a	31.90±4.64 ^a
Creatinine (mg/dL)	1.96±0.22 ^a	2.18±0.34 ^a	2.55±0.44 ^a	2.63±0.32 ^a	2.33±0.31 ^a
Calcium (mg/dL)	9.92±0.59 ^a	9.64±0.74 ^a	9.62±1.11 ^a	9.40±0.96 ^a	8.54±0.85 ^a
Phosphorus (mg/dL)	14.89±5.16 ^a	9.82±3.58 ^a	10.14±4.60 ^a	7.28±0.62 ^a	7.32±0.72 ^a
Potassium (mmol/L)	4.27±0.27 ^a	3.89±0.29 ^a	2.74±0.43 ^a	3.20±0.98 ^a	3.92±0.58 ^a
AF (UI/L)	222.03±60.47^a	169.48±32.26^a	143.12 ±31.65^a	167.62±39.59^a	188.17±53.04^a

Evaluated data	0 day	5 days	10 days	15 days	20 days
RBCs (x 10⁶/μL)	7.12±0.73^a	5.65±0.16 ^a	6.80±0.49 ^a	6.62±0.58 ^a	6.54±0.34 ^a
Hemoglobin (g/dL)	12.49±0.59^a	10.65±0.26 ^a	12.40±0.51 ^a	12.00±1.17 ^a	11.13±0.50 ^a
Hematocrit (%)	35.77±1.17^a	29.78±1.49 ^a	35.47±2.07 ^a	32.68±2.12 ^a	32.73±1.81 ^a
MCV (ﬂ)	51.80±3.15^a	52.93±1.11 ^a	52.83±2.75 ^a	50.05±3.31t^a	50.48±2.82 ^a
MCH (pg)	18.07±1.27^a	18.93±0.70 ^a	18.52±1.05 ^a	18.33±1.66 ^a	17.17±1.02 ^a
MCHC (%)	34.90±1.20^a	36.00±2.01 ^a	35.18±1.30 ^a	36.63±2.11 ^a	34.12±1.11 ^a
Platelets (x10³mm³)	321.17±65.94^a	358.75±45.06^a	329.00±29.76^a	341.75±56.8^a	441.66±66.44^a
Leukocytes (x103/µl)	15.43±4.77^a	14.15±2.58^a	22.47±4.63^a	18.95±3.25^a	14.22±2.49^a
Myelocytes (%)	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
Metamyelocytes (%)	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
Rods (%)	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.17±0.17^a
Segmented (%)	33.00±6.40^a	55.50±5.19 ^a	46.67±5.60^a	47.75±6.10 ^a	54.17±7.35^a
Eosinophils (%)	4.50±2.14^a	4.00±2.27 ^a	3.33±1.23^a	2.00±0.91^a	1.67±0.71^a
Basophils (%)	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
Typical Lymphocytes(%)	61.50±5.44^a	39.25±4.82 ^a	48.83±4.63^a	48.00±6.98^a	42.67±7.04^a
Monocytes (%)	1.00±0.37^a	1.25±0.25 ^a	1.17±0.40^a	1.25±0.75^a	1.33±0.61^a

Evaluated data	0 day	5 days	10 days	15 days	20 days
Density	1017±4.094^a	1012.8±2.24^a	1019.5±1.89^a	1008.66±1.76^a	1023.6±8.65^a
Ph	6.75±0.65^a	7.2±0.73^a	5.75±0.75^a	7.66±0.33^a	7.1±0.4^a
Glucose (mg / dl)	0±0^a	0±0^a	0±0^a	0±0^a	0±0^a
Ketone (mg / dl)	0±0^a	0±0^a	0±0^a	0±0^a	0±0^a
Nitrite (mg / dl)	0±0^a	0±0^a	0±0^a	0±0^a	0±0^a
Color	1.5±0.34^a	1±0^a	2±0.70^a	1±0^a	1.6±0.6^a
Aspect	0±0^a	0.4±0.4^a	0±0^a	0±0^a	0±0^a
Cells	0.5±0.22^a	0.4±0.29^a	0.5±0.28^a	0±0^a	0.2±0.2^a
RBCS	0±0^a	0±0^a	1.25±0.62^a	0±0^a	0±0^a
Leukocytes	0±0^a	0±0^a	0±0^a	0±0^a	0±0^a
Protein (mg / dl)	20±10^a	36±6^a	30±0^a	40±10^a	50±13.78^a
Cylinders	0.5±0.5^a	0.4±0.24^a	0.5±0.5ª	0.33±0.33^a	0.4±0.24^a
Mucus	0±0^a	0±0^a	0±0^a	0±0^a	0±0^a
Bacteria	0±0^a	0.6±0.6^a	0±0^a	0±0^a	0±0^a
Crystals	1.33±0.61^a	1.2±0.58^a	1.5±0.86^a	1±1^a	0.8±0.58^a

Days	Length(mm)	Diam(mm)	RI	BF (cm/s)	Length(mm)	Diam(mm)	RI	BF (cm/s)
	Left Kidney				Right Kidney
0	54.81±1.58 ^a	26.96±2.21^a	0.41±0.01^a	25.86±0.81^a	59.44±0.81^{a b c}	24.77±0.79^a	0.38±0.02^a	26.30±2.06^a
5	56.07±1.46 ^a	28.20±2.18^a	0.43±0.01^a	26.90±0.86^a	60.38±0.77^b	26.11±0.61^a	0.42±0.01^a	28.00±1.70^a
10	55.31±1.55 ^a	27.47±2.29^a	0.45±0.01^a	27.88±0.60^a	61.16±0.83^c	26.79±0.54^a	0.46±0.01^a	28.76±1.71^a
20	52.84±1.54 ^a	24.69±2.16^a	0.47±0.01^a	26.18±1.15^a	60.65±0.79^{a b c}	26.27±0.62^a	0.44±0.01^a	28.53±1.70^a

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS