Experimental model of Achilles tendon injury in rats

Forty male Rattus norvegicus albinus, Wistar lineage adult male weighing 250 to 300g were used for this experiment and thirty were surgically submitted to bilateral partial transverse section of the Achilles tendon. The right tendon was treated with radio waves (RF) whereas the left tendon served as control. On the third postoperative day, the rats were divided into four experimental groups consisting of ten rats each which were treated with monopolar RF adjusted to 650 kHz and 2w, for two minutes twice a week and a group of normal animals without any intervention, until they were sacrificed on the 7^th, 14^th and 28^th days, respectively. Tendons were weighed and collagen quantification was evaluated by hydroxyprolin content.

RESULTS:

Significant reduction in collagen content on day 7, 14 and 28 was related to control experiment to normal tendon (7 days, p<0.01; 14 e 28 days, p<0.05).

CONCLUSION:

The experimental model has been effective and available to be used to study Achilles tendon healing.

Models, Animal; Achilles Tendon; Collagen Type I; Hydroxyproline; Radio Waves; Rats

		NLT			SLT
Days	Median	Minimum	Maximum	Median	Minimum	Maximum	p
7	83.07	69.04	111	56.07	34.29	70.80	0.0003**
14	83.07	69.04	111	63.62	40.88	143.65	0.0114*
28	83.07	69.04	111	73.77	61.90	81.94	0.0218*

		NRT			SRT
Days	Median	Minimum	Maximum	Median	Minimum	Maximum	p
7	78.50	50.85	88.73	57.66	33.13	74.32	0.0003**
14	78.50	50.85	88.73	63.56	48.07	83.73	0.0114*
28	78.50	50.85	88.73	69.83	57.25	79.43	0.0218*

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

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[1] Correspondence: Carlos Augusto Real Martinez Laboratório de Investigação Médica Universidade São Francisco Avenida São Francisco de Assis, 218 12916-350 Bragança Paulista - SP Brasil carmartinez@uol.com.br

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Experimental model of Achilles tendon injury in rats1 1 Research performed at Laboratory of Medical Research-02, Division of Human Structural Topography, Faculty of Medicine of University of Sao Paulo (FMUSP), Brazil.

PURPOSE:

METHODS:

RESULTS:

CONCLUSION:

Experimental model of Achilles tendon injury in rats¹ 1 Research performed at Laboratory of Medical Research-02, Division of Human Structural Topography, Faculty of Medicine of University of Sao Paulo (FMUSP), Brazil.