The aim of this work was standardize technical norms to obtain a model of Walker 256 tumor in animals and get the tumorous cells number needed to increase the tumorous join percentage and longevity, it makes possible the research development in several health areas. The work was realized in two stages. In first were used 120 rats to crew’s training and technicals definitions. In second stage were used 84 rats, these separated in 7 groups (G) with 12 animals each one. The tumor, in ascitic form was inoculated on subcutaneous cellular tissue on dorsal of rats with the follow number of cells : GI, 1 x 10(7); GII, 5 x 10(6); GIII, 2,5 x 10(6); GIV, 1 x 10(6); GV, 5 x 10(5); GVI, 3 x 10(5) e GVII, 2 x 10(5). Were evaluate the join percentage and longevity in groups. The animals of GI, GII, GIII e GIV obtained 100% of tumorous development, however low longevity. The two GV and GVI had tumorous development often high that 90% and satisfactory longevity. The GVII didn’t show tumorous development . The conclusion was made all procedures must be exhaustivelly trained and the number of tumorous cells passable to inoculation, in subcutaneous cellular tissue of rats, must be in the range of 5x10(5) and 3x10(5) .
Disease models animal; Neoplasms experimental