Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion 1 1 Research performed at Laboratory of Experimental Surgery, and Laboratory of Pharmacology, Universidade Federal de São Paulo (UNIFESP), Brazil. Part of PhD degree thesis, Postgraduate Program in Translational Medicine. Tutor: Itamar Souza de Oliveira Junior.

Mieko Okada Luiz Fernando Reis Falcão David Ferez José Luiz Martins Paolo Ruggero Errante Francisco Sandro Menezes Rodrigues Afonso Caricati-Neto Márcia Marinho Guilherme Fenelon Itamar Souza Oliveira-Júnior About the authors

Abstract

Purpose:

To investigate the effects of atenolol in inflammatory mediator and oxidative stress in a myocardial injury by intestinal ischemia/reperfusion in rat model.

Methods:

Adult Wistar male rats were randomly (n=8), anesthetized and divided in: Sham: submitted to operation only; group SS+IR: intravenous saline infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); group AT+IR: intravenous atenolol infusion (2 mg/kg) following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); and group AT+I+AT+R: intravenous atenolol infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and in the time 45 minutes other atenolol doses were administrated and the artery was open for 120 minutes (reperfusion), all animals were submitted to muscular relaxation for mechanical ventilation. In the end of experiment the animals were euthanized and the hearts tissue were morphology analyzed by histology and malondialdehyde by ELISA, and the plasma were analyzed for tumor necrosis factor-alpha by ELISA.

Results:

The group SS+IR demonstrated the higher malondialdehyde levels when compared with the atenolol treated-groups (p=0.001) in the heart tissue. The tumor necrosis factor-alpha level in plasma decrease in the treated groups when compared with SS+IR group (p=0.001). Histology analyses demonstrate pyknosis, edema, cellular vacuolization, presence of inflammatory infiltrate and band contraction in the heart tissue of the rats.

Conclusion:

Atenolol significantly reduce the degree of cardiac damage after intestinal ischemia-reperfusion.

Key words:
Adrenergic Antagonists; Atenolol; Ischemia; Reperfusion; Cytokines; Oxidative Stress; Rats.

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia Al. Rio Claro, 179/141, 01332-010 São Paulo SP Brazil, Tel./Fax: +55 11 3287-8814 - São Paulo - SP - Brazil
E-mail: sgolden@terra.com.br