Acessibilidade / Reportar erro

Transforming growth factor beta1 (TGF-ß1) levels in a rat model of induced pleural empyema 1 1 Research performed at Experimental Research Center, and Division of Thoracic Pediatric Surgery, Service of Pediatric Surgery, Hospital de Clínicas of Porto Alegre (HCPA), School of Medicine, Universidade Federal do Rio Grande do Sul (UFRGS). Part of Master degree thesis, Postgraduate Program in Medicine, UFRGS. Tutor: Prof. José Carlos Fraga.

Abstract

Purpose:

To evaluate the concentration of transforming growth factor beta 1 (TGFB1) levels in a rat pleural effusion obtained by inoculation of intrapleural bacteria or turpentine through thoracentesis.

Methods:

Thirty-Nine Wistar rats were divided into three groups: Staphylococcus aureus (SA, n = 17); Streptococcus pneumoniae (SP, n = 12); and turpentine (control, n = 10). Pleural fluid was collected through ultrasound-guided thoracentesis 12 h, 24 h, and 36 h after instillation of bacteria or turpentine. Levels of TGFB1 were measured in pleural fluid.

Results:

At 12 h, mean TGFB1concentrations were 5.3450 pg/mL in the SA group, 5.3449 pg/mL in the SP group, and 5.3450 pg/mL in controls. At 24 h, they were 4.6700 pg/mL in the SA group, 4.6700 pg/mL in the SP group, and 4.6700 pg/mL in controls. At 36 h, they were 4.6699 pg/mL in the SA group and in control. No difference was observed among the groups in mean TGFB1concentration (p = 0.12); however, a significant intragroup reduction in mean TGFB1 was observed between 12 and 24 h (p < 0.01).

Conclusion:

The transforming growth factor beta 1 concentrations were not useful as a diagnostic tool or an early marker of infected pleural effusion.

Key words:
Empyema, Pleural; Cytokines; Transforming Growth Factor beta1; Rats.

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com