Hypertonic glucose solution 10 %-25 % on the mesenterium and peritoneum of the rat : macroscopic and microscopic study

Purpose: The objective of the experimental study is to detect the macroscopic and microscopic alterations of the mesenterium and parietal peritoneum when hypertonic glucose aqueous solution 10%-25% is administrated into the peritoneal cavity of the rat. Methods: 90 Wistar females young rats adults were used weighin between 180-250 g, numbered 1 to 90, establishing unique group and divided in three groups (A, B, C) of 30 animals chosen aleatory manner. 0,9% saline solution was used called control group, or group A, 10% glucose solution named group B, and in the others 30 was used 25% glucose solution named group C, differing in the observation period, (06h, 24h and 48h), but with the same procedure. A midline abdominal wall laparotomy was made and in the animals of the control group was injected 2 ml of a 0,9% saline solution into the peritoneal cavity. After, we made a suture in mass without to include the peritoneum. For the others groups (B, C) the rats received 10% glucose solution and 25% glucose solution injected into the peritoneal cavity respectively. All groups were kept under observation and the results were submitted to statistical analysis by a longitudinal and transversal comparative study. Results: A new surgery was done in 6h, 24h and 48h, and we observed in macroscopic evaluation, the presence of fluid, serous uniforme and rosy all over the cavity. Vascular congestion was present. We dried out 90 fragments of mesenterium and 90 fragments of parietal peritonium bilateral. In the microscopic study, necrosis was not present. For the mesenterium histological study we observed 16 cases (17,8%) unspecific chronic inflammation, 30 cases (33,4%) hiperplasic linfonod, 10 cases (11,1%) high vascular congestion, 6 cases (6,6%) reaction fibrosis and 28 cases (31,1%) no alteration. For the parietal peritonium histological study we observed 6 cases (3,3%) reaction fibrosis and 174 cases (96,7%) no alteration. Giant cell was not present. In the statistical analisys statistic there is no significance between the groups (p>0,05). Conclusion: Hypertonic glucose solution and NaCl 0,9% on the mesenterium and parietal peritonium do not produce tissue necrosis in a rat and the inflammation process has the same intensity.


Introduction
The peritoneal socket is vulnerable to the chemical, bacterial and traumatic aggressions; fragile, therefore, the tissue integrity of the mesenterium and the peritoneum.It enters the illnesses of the peritoneum, the peritonitis is most frequent.The peritoneum plays important role in the protection of the abdominal socket.In the execution of this function, it makes possible the absorption, exudation and capacity to form tacks, when it is headquarters of aggressions for traumatic agent or infectious 1 .The job of sugar in the treatment of infected wounds has been used for centuries.Egyptian surgeons used honey and other boiled sugary syrups in the treatment of wounds and burns 2 .The use of the glycoside hypertonic solution 10% in the laundering of the peritoneal socket, in experimental study, the validity of a peritoniti for injury of colon, determine a faster intestinal healing and resistant comparatively to the use of the 0.9% saline solution 3 .The use of solution of dextrose diluted 10% in water distilled in the same rat for the removal of the mucinous content of the tumor in the intra-peritonei irrigation, as mucolytic agent, in the surgical treatment of pseudomyxoma, prevents the intestinal blockages 4 .This study was made for observing the use of the glycoside hypertonic solution 10% and 25% in the peritoneal socket and if the crystals contained in these chemical solutions would cause injury to the mesenterium and parietal peritoneum.Thus being, valid to point out that in consulted literature it has registered on the use of these hypertonic glycoside solutions into the peritoneal socket, however, had not been evidence references of works that detected the morphologic alterations (macroscopic and microscopic) of the mesenterim and the parietal peritoneum when submitted to the laundering with watery hypertonic glucose solution, without mentioning stories of tissue injury to the celomatic content.These facts in had motivated them to the present research.Methods 90 young rats "Wistar", adults had been selected, with weight varying of 180-250g, originating the breeding Lab Surgery of the Center of Experimental Surgery of the University Foundation of Sciences of the Health of Alagoas (UNCISAL), and the surgical procedures had obeyed the criteria, norms techniques and international laws of research in animals of the Committee of Ethics by Colégio Brasileiro de Experimentação Animal (COBEA) with approval of the Advice of Ethics of the same Institution ( The Brazilian Association for Animal Studies.

Experiment design
The sample was numbered 1 to 90, constituting on groups randomly distributed in 3 sub-groups (A, B and C) each one contains 30 animals with identical procedures, differing only in the period of observation, receiving water and ration ad libitum.The numbers 1 through 30 constitute of group A or group-control (NaCl 0.9%),numbers 31 through 60 constitute group B or group of glycoside hypertonic solution 10%; and numbers 61 through 90 constitute group C or group of glycoside hypertonic solution 25%.After weighing each animal was anesthetically induced, enclose a cotton ball with fixed weight (3,0 g), impregnated with 7 ml of diethyl ether, saturated previously, for period of 1 the 4 minutes.The induction was carried through until reaching the anesthesia level (without movements of head, extremities and body; with disappearance of the interdigital reflexes, normal breath and rosy extremities). 5Continuously maintaining the anesthetic level with anesthetic induction tube of 10 cm of length for 2,5 cm of diameter in semi closed system.The anesthetized animal was carried to the operation table with thichtomy of the coats of the abdominal area, using an arched shears (with the purpose to prevent injuries of the skin); setting of the legs in the extremities of the plate in position dorsal decubitus, and antisepsis of the skin, the same region with Iodine solution 10%.The position of the fenestrated cloth, sterilized, delimiting the area to be operated.

Dissection of the abdominal region
A laparotomy with longitudinal medium incision of theskin, two centimeters below of the Xiphoideus sterni process was made, with scalpel blade nº 23, extending itself for three centimeters caudal in the measured ventral line, understanding the cellular composition of the subcutaneous, aponeurosis of the abdominal straight muscles, exposing thus the peritoneum parietal.An opening and incision exactly of the peritoneal socket, where its inspection was fulfilled.The choice of the procedure carried through for the introduction in the peritoneal socket of the saline solution 0.9% (NaCl), hypertonic glucose 10% and hypertonic glucose 25%, as also the choice of these animals for the respective groups: Control, Glucose 10% and Glucose 25%; she was random, before the beginning of the procedures, being these groups, operated and re-operated with 6h, 24h and 48h, in accordance with the sequence that was carried out through the experiments.There were 90 cards numbered in the order of 1 to 90, each one contains the groups to be studied, group-control (NaCl 0.9%), group B or group-glucose 10% and group C or group-glucose 25%.After that, these cards had been ranks in cloudy and closed a ballot box, where an assistant, randomly, removed each card which would correspond, in data, to the animals that would compose the three sub-groups of 30 animals (A, B and C).Soon after, each sub-group of 30 rats was submitted the new random choice for subdivision of the sample in 6h, 24h and 48h.Group: The rats of the groupcontrol had received 2 ml from a solution of NaCl 0.9%, introduced directly in all the quadrants of the peritoneal socket.Disposable 3ml syringe was used.Later a synthesis in mass was done of the abdominal region measured by simple suture type, using wire of mononylon 3-0, keeping away from the parietal peritoneum.Groups B and C: The rats of the group-glucose after laparotomy, had received 2 ml from a hypertonic glucose solution 10% and 25%; introduced directly in the peritoneal socket and distributed in all the quadrants.Later a synthesis in.mass of the Regio abdominis measured following the criteria for the rats of the group-control.

Acute and chronic inflammatory reaction
The removed material was placed for setting during 36h in formaldehyde 10%, for the dehydration, had been used four alcohols in changeable degrees of dehydration: 1º 70%; 2º 90% and 2 in absolute alcohol.This material was dyed by the Hematoxilina and Eosina, in 30 second (HE).One verified presence and intensity of the reactions inflammatory and written down predominant the cellular elements: hyperplasc of linfonods, reaction fibrosis, vascular congestion and unspecific chronic inflammation.

Results
The operations proposals had been carried through adequately, not having occurred difficulty technique, as well as had not occurred trans-operative complications.The animals had presented good healing, without serous or purulent secretion elimination for the operated wounds.Microscopical findings for the mesenterium for group: Group A-The hyperplasic linfonods had appeared in 12 cases with 40% percentage; reaction fibrosis, 4 cases with 13,3% percentage; vascular congestion, 2 cases with 6,7% percentage; unspecific chronic inflammation, 4 cases with 13,3% percentage; 8 cases without alteration, with 26,7% percentage.There were a total of 30 cases with 100% percentage.Group B -The hyperplasic linfonods had appeared in 8 cases with 26,7% percentage; reaction fibrosis, 2 cases with 6,7% percentage; vascular congestion, 6 cases with 20,0% percentage; unspecific chronic inflammation, 4 cases with percentage of 13,3% and 10 cases without

Postoperative care
The animals, warmed in artificial light during the aesthetical recovery, had remained in double river steamers under the same conditions of the daily pay-operator, in the Laboratory of Experimental Surgery.All the animals had received the daily clinical evaluation in postoperative during the evolution from the experiment, being the findings registered in individual records.The clinical evolution of these animals was characterized as good in the absence of complications; to regulate when suppuration occurred and partial or total dehiscence of the skin; harm when the outcome was the death of the animal.

Variable studies
Macroscopic -After anesthesia with diethyl ether, magnifying the previous incision of the abdominal wall, in the direction skull-volume, of the Xiphoideus sterni process to the pubis, enough ample for the comments of the structures to be studied: mesenterium and parietal peritoneum.In the peritoneal socket, we observe the presence of liquid with uniformity of the serosal and the coloration was rosy in all extension of the area and studied place.We also noticed an abundant presence of vascular congestion.These fabric blocks had been struck laterally, allowing to the comment and photographic documentation.Later the section of these structures was continued.In the mesenterium, removed it of a total of 90 fragments was measuring greater 2,5 X 2,0 X 1,8 cm and the minor 1,7 X 1,2 X 1,0 cm, irregular form or oval, yellowish external surface with white traces.It was completed removed it of the surgical parts of the parietal peritoneum, bilateral, totalizing 180 fragments measuring the greater 2,3 X 1,3 X 1,0 cm and the minor 1,2 X 0,8 X 0,6 cm, all of irregular form and external surface of clear coloration chestnut and firm consistency.The parts had been analyzed following previous protocol; the animals had been submitted to euthanasia for inhalation of lethal dose of diethyl ether.Microscopy of the mesenterium -the cuts of the fragments of fabric of this region had shown to infiltrations of lynfocites and some plasmocites.One associated, presence of arterioles and capillaries presenting ecstasis and vascular congestion, characterizing histological picture of chronic inflammation process unspecific.Presence of linfonods was proven, presenting proliferation of lynfocites elements and reticular cells, designating histological picture of linfo-reticular hyperplasia.It was developed, diffuse fibroblastic proliferation, without inflammation, resulting only in fibrosis reacional.The excessively fragments ones had been presented without histological alteration (Figure 1).Microscopy of the parietal peritoneum -In the pathology of the material of the parietal peritoneum, in the cuts of the fragments of fabric, only fibroblastic proliferation with formation of collagen excess was observed constituting reacional typical histological picture of fibrosis, beyond fragments without histological alteration (Figure 2).Totalizing 30 cases and p 0,05, that is, without significance (Tables 1 and 2, Figures 3 and 4).To the microscopy of the peritoneum it disclosed to 6 cases with fibrosis (3,3%) and 174 cases (96,7%) without histological alteration reaction (Table 3, Figure 5).

Discussion
In the present work, we detected the macroscopic and microscopic alteration of the mesenterium and the parietal peritoneum when we manage the watery glucose solution 10% and 25% in the peritoneal socket of rat "Wistar", and statistical study, where a sample of 90 animals, subdivided in 3 groups of 30 animals and the comparison of the histological reactios of the mesenterium and the parietal peritoneum was analyzed.One chose adult young and females, without taking in consideration possible differences in the healing process or factor of increase 6 .the animals had been submitted the anesthesia with diethyl ether under inhalation, did not observe aesthetical complications.As chosen anatomical region, longitudinal incision of 3 cm in the regio abdominis was measured with tecidual resection until the parietal peritoneum 7 .All these animals had been re-operated in 6h, 24h and 48h, for being an animal that possess a capacity of fast tecidual regeneration 8 .Practiced synthesis in.mass, by simple suture, abstaining the parietal peritoneum "ipsi-lateral" with the purpose not to allow that the wire used for the suture intervened with the process of peritoneal healing.What it was confirmed in analyzes histological, as also to prevent  extravasation of the introduced intraperitoneal.It did not have risk of the animals to cause traumatism to the proper one abdomen or to remove the wire of suture of the operated wound, in virtue of previous comment of these rodents, to the odor of the plolivinilpirrolidona-iodine solution 10%, being applied to the wound, daily, when necessary.In literature, references of diluted watery works had been evidenced using itself solutions of hypertonic glucose in water distilled in the same ratio, for intraperitoneal laundering as mucolitic agents in the removal of the mucinous content of pseudomyxoma, thus preventing the intestinal blockages subsequently. 9 10 11In relation to the type of boarded glycoside hypertonic solution in the research, we choose the glucose solutions 10%, due to the works of evidence in the consulted literature, praising the use of the glucose solution 10% in the laundering of the peritoneal socket in experimental study, in the validity of a peritonit for injury of colon, promoting faster and resistant intestinal healing, comparatively the use of the salty solution 0.9%, 12 we remember that in literature does not have references to the tecidual necrosis in the mesenteric and the following parietal peritoneum to the use and introduction of glycoside hypertonic solution managed 10% in the peritoneal socket.We prioritize the use of the glycoside solution 10%, in reason of the mentioned authors to have if used of this hypertonic solution in the same concentration, however, with adverse purposes.The glycoside hypertonic solution was managed in the peritoneal socket 25%, in the expectation not to have tecidual necrosis, a time that the same one did not register when of the use of the glycoside solution 10%.In the clinical evolution of the postoperative and in the inspection of the abdominal wall, injuries had not been evidence that could intervene with the evolution of this work.Thus being, in our histological analysis, we noticed that the inflammation reaction becames more evident in presence of the saline solution 0,9% when of the subdivided of this study, comparing itself it the hypertonic glucose solutions 10% and 25%.However, in the global analysis, it was verified that the presented intensity of the inflammation reaction process independent of the used solution, remaining, therefore, these values as not significant.Predominant the histological findings had been: hyperplasic reaction of linfonods, fibrosis, vascular congestion and unspecific chronic inflammation, these had been studied comparing the samples between the groups and time of re-operation.
Using the accurate test of Fisher, p>0,05, was concluded that, therefore not significant in this research.We call attention for the fact that the number of hyperplasic linfonods to persist with bigger intensity in the histological study of the mesenteric; not occurring the same in the fragments of the parietal peritoneum for being the mesenteric small farm of the bigger numbers of linfonods.It is seen, no meanwhile, that the inflammatory process of the chemical irritation does not have trend to progress, but yes, to decrease in short term.Although the results of the present research be able to obvious, they will be necessary complementary studies that involve hypertonic glucose solutions in the mesenterium and the parietal peritoneum, objectifying to elucidate and to supply new given that will be able to define the mechanism of action of these substances yet not completely understood.

FIGURE 3 -
FIGURE 3 -Distribution of histological alterations of fragments of the mesenterium for group.

FIGURE 4 -
FIGURE 4 -Distribution of the histological alterations of fragment of the mesenterium for group and time of re-operation.

FIGURE 5 -
FIGURE 5 -Percentage of the global distribution of histological alterations of fragments of parietal peritoneum.

TABLE 1 -
Distribution of histological alterations of fragments of the mesenterium for group.

TABLE 2 -
Distribution of the histological alterations of the mesenterium for group and time of reoperation.

TABLE 3 -
Global distribution of histological alterations of fragments of parietal peritoneum.