Hydrogel-based dressings in the treatment of partial thickness experimentally induced burn wounds in rats

Bernardes, Milton Junior Cândido; Gonçalves, Randys Caldeira; Carvalho, Carolyna de Sousa; Rosa, Luciana Martins; Ferreira, Amanda Peixoto; Vilela, Marielle Sousa; Vinaud, Marina Clare; Galdino Junior, Hélio; Lino Junior, Ruy de Souza

doi:10.1590/acb370401

Milton Junior Cândido Bernardes

PhD. Universidade Federal de Goiás – Tropical Pathology and Public Health Institute – Postgraduation Program in HostParasite Relationship – Goiânia (GO), Brazil.

http://orcid.org/0000-0001-7346-0786

Randys Caldeira Gonçalves

PhD. Universidade Federal de Goiás – Tropical Pathology and Public Health Institute – Postgraduation Program in HostParasite Relationship – Goiânia (GO), Brazil.

http://orcid.org/0000-0003-3675-9628

Carolyna de Sousa Carvalho

MSc. Universidade Federal de Goiás – Tropical Pathology and Public Health Institute – Postgraduation Program in HostParasite Relationship – Goiânia (GO), Brazil.

http://orcid.org/0000-0003-4029-7518

Luciana Martins Rosa

Graduate student. Universidade Federal de Goiás – Goiânia (GO), Brazil.

http://orcid.org/0000-0003-2780-1185

Amanda Peixoto Ferreira

Graduate student. Universidade Federal de Goiás – Goiânia (GO), Brazil.

http://orcid.org/0000-0002-3778-0819

Marielle Sousa Vilela

Nurse. Universidade Federal de Goiás – Nursing School – Goiânia (GO), Brazil.

http://orcid.org/0000-0003-4847-4056

Marina Clare Vinaud

PhD. Universidade Federal de Goiás – Tropical Pathology and Public Health Institute – Biosciences Department – Goiânia (GO), Brazil.

http://orcid.org/0000-0002-6746-9191

Hélio Galdino Junior

PhD. Universidade Federal de Goiás – Nursing School – Goiânia (GO), Brazil.

http://orcid.org/0000-0002-5570-8183

Ruy de Souza Lino Junior^*Corresponding author: ruy@ufg.br | (55 62) 3209 6163

PhD. Universidade Federal de Goiás – Tropical Pathology and Public Health Institute – Biosciences Department – Goiânia (GO), Brazil.

http://orcid.org/0000-0002-0572-5102

Conflict of interest: Nothing to declare.

Corresponding author: ruy@ufg.br | (55 62) 3209 6163

Authors’ contribution

Substantive scientific and intellectual content of the study: Bernardes MJC, Galdino Junior H, and Lino Junior RS; Conception and design the study: Junior RS; Acquisition, analysis and interpretation of data: Bernardes MJC, Gonçalves RC, and Carvalho CS; Acquisition of data: Rosa LM, Ferreira AP, and Vilela MS; Technical procedures: Bernardes MJC, Gonçalves RC, Carvalho CS, Rosa LM, Ferreira AP, and Vilela MS; Histopathological examinations: Bernardes MJC, Gonçalves RC, Carvalho CS, Rosa LM, and Ferreira AP; Statistical analysis: Bernardes MJC; Manuscript preparation: Bernardes MJC, and Vinaud MC; Manuscript writing: Vinaud MC; Critical revision: Vinaud MC, and Galdino Junior H; Final approval of the version to be published: Lino Junior RS.

DAI	G1 (n=15) Medium ± SD	G2 (n=15) Medium ± SD	G3 (n=15) Medium ± SD	G4 (n=15) Medium ± SD	G5 (n=15) Medium ± SD	G6 (n=15) Medium ± SD	p-value	Tukey
14	24.9±12.8	47.9±22.8	49.1±17.2	61.5±14.8	35.9±16.8	44.7±11.8	0.02^* * statistical difference;	G4>G1
30	91.1±3.7	86.4±3.1	85.7±4.4	86.1±6.9	92.6±10.7	91.4±10.3	0.32	G4>G1

Pathologic process	DAI	G1 (n=15) Median (min-max)	G2 (n=15) Median (min-max)	G3 (n=15) Median (min-max)	G4 (n=15) Median (min-max)	G5 (n=15) Median (min-max)	G6 (n=15) Median (min-max)	p-value	Dunn’s
Necrosis/crust	7	3 (2-3)	3 (2-3)	1 (0-2)	2 (2-3)	3 (2-3)	1 (1-2)	0.002^* * statistical difference.	G1>G3; G1>G6; G5>G3; G5>G6
	14	2 (1-3)	3 (2-3)	1 (0-1)	1 (0-1)	1 (1-2)	1 (0-1)	0.002^* * statistical difference.	G1>G3; G1>G4; G2>G3; G2>G4; G2>G5; G2>G6
	30	2.5 (2-3)	0 (0-1)	0 (0-1)	1 (0-1)	1 (1-2)	0 (0-1)	0.003^* * statistical difference.	G1>G2; G1>G3; G1>G4; G1>G6; G5>G6
Granulation tissue	7	2 (1-2)	1 (1-2)	1.5 (1-2)	3 (2-3)	1 (1-2)	3 (2-3)	0.003^* * statistical difference.	G4>G2; G4>G3; G4>G5; G6>G2; G6>G5
	14	2.5 (2-3)	2 (1-3)	2 (2-3)	2.5 (2-3)	3 (2-3)	3 (2-3)	0.512
	30	0 (0-0)	0 (0-1)	0 (0-1)	0 (0-0)	0 (0-0)	0 (0-0)	0.202
Re-epithelialization	7	0 (0-0)	0 (0-0)	0 (0-0)	0 (0-0)	0 (0-0)	0 (0-0)	1.00
	14	0.5 (0-2)	0 (0-2)	2 (1-2)	2 (2-2)	2 (2-3)	1.5 (1-2)	0.014^* * statistical difference.	G5>G1; G5>G2
	30	3 (2-3)	3 (3-3)	3 (3-3)	3 (3-3)	2 (2-3)	3 (2-3)	0.013^* * statistical difference.	G2>G5; G3>G5

Pathologic process	DAI	G1 (n=15) Median (min-max)	G2 (n=15) Median (min-max)	G3(n=15) Median (min-max))	G4 (n=15) Median (min-max)	G5 (n=15) Median (min-max)	G6 (n=15) Median (min-max)	p-value	Dunn’s
Necrosis/crust	7	2 (1-3)	1 (1-2)	0 (0-1)	1 (1-2)	1.5 (1-2)	1 (1-3)	0.041^* * statistical difference.	G1>G3; G6>G3
	14	1 (1-1)	2 (1-3)	1 (1-1)	1.5 (1-2)	1 (0-2)	0 (0-1)	0.021^* * statistical difference.	G2>G6; G4>G6
	30	0 (0-1)	1 (0-1)	0.5 (0-1)	0 (0-1)	1 (0-2)	0 (0-0)	0.134
Hemorrhage	7	1.5 (0-2)	2 (1-2)	2 (0-3)	1 (1-2)	2.5 (2-3)	3 (2-3)	0.010^* * statistical difference.	G6>G1
	14	0 (0-0)	1 (1-2)	1 (0-2)	1 (0-1)	1 (0-2)	1.5 (0-2)	0.033^* * statistical difference.	G6>G1
	30	0 (0-0)	0 (0-0)	0 (-0-0)	0 (0-0)	0 (0-0)	0 (0-0)	1.000
Fibrin	7	1 (1-2)	2 (2-2)	0 (0-2)	1 (1-2)	1 (1-2)	1 (0-2)	0.013^* * statistical difference.	G2>G3; G2>G6
	14	1 (0-1)	1 (1-2)	1 (0-3)	1 (0-2)	0 (0-1)	0 (0-1)	0.100
	30	0 (0-0)	0 (0-0)	0 (0-0)	0 (0-0)	0 (0-0)	0 (0-0)	1.000
PMN	7	1.5 (1-2)	1 (1-2)	0 (0-1)	1 (1-2)	1.5 (1-2)	1 (1-3)	0.005^* * statistical difference.	G6>G3
	14	1 (1-1)	1.5 (1-2)	1 (1-1)	2 (2-2)	1 (1-3)	0 (-01)	0.005^* * statistical difference.	G2>G6; G4>G6; G5>G6
	30	0 (0-1)	1 (0-1)	0 (0-1)	0 (0-0)	1 (0-1)	0 (0-1)	0.063
MN	7	2 (2-3)	3 (3-3)	2 (1-3)	3 (2-3)	3 (3-3)	2 (2-2-)	0.005^* * statistical difference.	G2>G6; G5>G6
	14	2 (2-3)	2 (2-2)	2 (1-3)	2.5 (1-3)	3 (2-3)	2 (2-3)	0.534
	30	1 (1-2)	2 (1-2)	1 (1-2)	1 (1-2)	1.5 (1-2)	1 (1-1)	0.321^* * statistical difference.
Angiogenesis	7	3 (3-3)	3 (3-3)	3 (3-3)	3 (3-3)	3 (3-3)	3 (3-3)	1.000
	14	3 (2-3)	2 (2-2)	3 (2-3)	3 (3-3)	3 (2-3)	3 (2-3)	0.012^* * statistical difference.	G1>G2; G4>G2; G6>G2
	30	1 (1-1)	1 (1-2)	1 (1-1)	1.5 (1-2)	2.5 (1-3)	1 (1-1)	0.040^* * statistical difference.	G5>G1; G5>G3; G5>G6
Fibroblast	7	3 (3-3)	3 (3-3)	3 (3-3)	3 (3-3)	3 (3-3)	3 (3-3)	1.000
	14	3 (3-3)	3 (3-3)	2.5 (2-3)	3 (3-3)	3 (2-3)	3 (2-3)	0.175
	30	2 (2-3)	2 (2-2)	2 (2-2)	2 (1-2)	2,5 (2-3)	1 (1-2)	0,022^* * statistical difference.	G1>G6; G5>G6

Wound closure – 30 DAI	G1	G2	G3	G4	G5	G6	p-value
Total	5	5	5	5	5	5
Yes	4	4	4	4	1	3	1.00
No	1	1	1	1	4	2

Microorganisms presence – 7 DAI	G1	G2	G3	G4	G5	G6	p-value
Total	5	5	5	5	5	5
Yes	0	0	0	0	0	3	1.00
No	5	5	5	5	5	2

DAI	G1 (n=15) Median (min-max)	G2 (n=15) Median (min-max)	G3 (n=15) Median (min-max)	G4 (n=15) Median (min-max)	G5 (n=15) Median (min-max)	G6 (n=15) Median (min-max)	p-value	Dunn’s
30	0.02 (0.00-4.25)	4.26 (0.12-18.73)	9.97 (0.13-38.27)	7.47 (0.25-30.13)	8.04 (0.71-31.70)	6.30 (0.04-20.45)	0.001^* * statistical difference.	G2>G1; G3>G1; G4>G1; G5>G1; G6>G1
7	0.03 (0.00-1.51)	0.74 (0.00-15.03)	0.28 (0.00-3.72)	1.49 (0.02-16.03)	0.20 (0.01-3.87)	0.72 (0.00-17.13)	0.001^* * statistical difference.	G2>G1; G3>G1; G4>G1; G5>G1; G3>G2; G5>G2; G4>G3; G6>G3; G6>G4
14	0.02 (0.01-5.02)	1.79 (0.02-15.62)	3.20 (0.02-17.31)	2.13 (0.09-23.32)	3.21 (0.02-19.82)	1.67 (0.03-17.59)	0.001^* * statistical difference.	G2>G1; G3>G1; G4>G1; G5>G1; G6>G1; G6>G3; G6>G5

[1] Conflict of interest: Nothing to declare.

Brasil

Brasil

Hydrogel-based dressings in the treatment of partial thickness experimentally induced burn wounds in rats

ABSTRACT

Purpose:

Methods:

Results:

Conclusions: