Indirect hormonal evaluation and study of the follicles preservation in the ovarian transplantation tissue in rats inguinal region

PURPOSE: To analyse the effectivity technique of the subcutaneous autologous bisseccionated ovarian transplantation technique in inguinal region of the albine rats, close to the femoral vases, through the indirect study of hormonal production, made by the evaluation of the citology of the vaginal smears and the direct study of the tissue viability, carried out by histologic analysis. METHODS: 30 rats were analysed which suffered bilateral ooforectomy, being 15 of them intended for control and 15 of them suffered the ovarian transplantation. After 28 days, the grafting were recovered and evaluated. The analysis of the cytology of the vaginal smears were made in the moment 0, that is to say, pre-transplantation, and in the moment 1, post-transplantation, for all the animals and the histologic evaluation was accomplished at the moment 0 for the two groups and at the moment 1 for the animals that suffered transplantation. RESULTS: The results of the experiment demonstrated the viability and functionality of the grafts in 100% of the laminas evaluated, that were observed through the vaginal smears in estrous phase to all the rats of the experience group and by the presence of follicles in various stages of maturation, observed in the recovered grafts, indicating, directly, the viability of the ovarian tissue. CONCLUSION: Allowing to conclude that the ovarian transplantation is a viable alternative, in the conditions of the experiment, to the hormonal and follicular preservation in rats.

Ovary; Transplantation; Hormones; Estrogens; Progesterone; Rats

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